CAS 1135-40-6 CAPS Buffer 3-Cyclohexylaminopropanesulfonic Acid

application and configuration of CAPS；CAS 1135-40-6 CAPS;Good buffer soluton

How much do you know about the application and configuration of CAPS
The full name of CAPS is 3-cyclohexylaminopropanesulfonic acid. The appearance is white powder crystal particles, which can be dissolved in water but the dissolution rate is slow. The CAS number is 1135-40-6 and the molecular formula is C9H19NO3S. It is a biological buffer and is mostly used in biochemical diagnostic kits, PCR diagnostic kits, DNA extraction kits and RNA extraction kits. But what other applications besides these places? The following will introduce the applications of CPSA.

Brief introduction

1. CAPS is used as a biological buffer: CAPS is mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as a buffer for enzymatic chemistry and HPLC separation of alkaline drugs. CAPS can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at a pH of 10.5. It can also be dissolved in deionized water and adjusted to pH 11.0 for purification of fibronectin.
CAPS needs better purity when used as a biological buffer, and generally requires analytical purity. When used in industry, the purity requirements are relatively low, but different treatments must be made for different applications.
2. CAPS is used in industry: used for new coatings and new materials. CAPS is also the raw material for manufacturing welding materials, air conditioning equipment and manufacturing metal lithium.
3. Used as analytical reagent, heat exchange carrier, and also in the pharmaceutical industry. For air conditioning, it is also used to make fireworks, dry batteries and metal lithium, and also used as flux and desiccant.
4. For the coating industry, it is used as a water-based isocyanate curing agent. The water-based isocyanate curing agent can coexist stably with resins containing active groups (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time at room temperature.
The CAPS application is roughly that, the following will introduce the conventional configuration method of CAPS
Since the CAPS buffer is mainly composed of CAPS and deionized water, adjust the pH value with sodium hydroxide to avoid contact with air. Add deionized water to CAPS to 900ml, adjust the pH to 11.0 with 2mol/L NaOH, then dilute to 1L and store at 4ºC. Generally, a buffer with a high methanol concentration has a good effect on the transfer of low molecular weight proteins, while a buffer with a low methanol concentration or even no methanol is conducive to the transfer of high molecular weight proteins. The concentration of methanol in the CAPS electro-imprinting buffer is 0-20%, so the CAPS electro-imprinting buffer is more suitable for the transfer of low molecular weight proteins.
Hubei Xindesheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents, luminescent substrates and other products. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many enterprises. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.