CAS # 1132-61-2 99% Mops Good Buffer Solutions 3-Morpholine Propane Sulfonic Acid

Basic information

Product description

 

Product packaging

 

Configuration of MOPS solution (for reference )

 

The MOPS buffer is 10 × concentrated and the working concentration is 1 ×. It can be directly diluted by 10 times with RNase-free distilled water (DEPC treated water).

 

Usage

As a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of thin-layer chloroplast preparation. It can protect oxyhemoglobin from oxidizing to methemoglobin during freeze-drying process, and can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.

Advantage

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

Characteristics and application of Good's buffer MOPS

MOPS (3-morpholine propane sulfonic acid) is a kind of zwitterionic buffer, namely Good's buffer, is a series of N-substituted amino sulfonic acid, has good pH stability, high polarity, and is suitable for many chemical reagents. And enzymes are inert, do not participate in or interfere with the process of biochemical reactions, and have no inhibitory effect on enzymatic chemical reactions, so it can be specially used for the research of organelles and extremely variable, pH-sensitive proteins and enzymes.

Basic information

In addition, MOPS buffer also has the following characteristics common to Good's buffer system:

1. The pKa value is between 6-8;

2. High solubility in water;

3. It is not easy to penetrate the biofilm;

4. The salt effect is small;

5. Ion concentration, solution composition and temperature have little effect on dissociation;

6. Does not form complexes or precipitates with metal ions;

7. The buffer is chemically stable;

8. For the absorption of 0.1mol·L aqueous solution in the ultraviolet region, the absorbance at 260 and 280nm wavelengths is as small as possible;

9. Easy to make high-purity salt.