CAS 1135-40-6 CAPS Good Buffer 3-(Cyclohexylamine)-1-Propanesulfonic Acid

CAS 1135-40-6 CAPS Buffer 3-(Cyclohexylamine)-1-Propanesulfonic Acid

How much do you know about the application and configuration of CAPS
The full name of CAPS is 3-cyclohexylaminopropanesulfonic acid. The appearance is white powder crystal particles, which can be dissolved in water but the dissolution rate is slow. The CAS number is 1135-40-6 and the molecular formula is C9H19NO3S. It is a biological buffer and is mostly used in biochemical diagnostic kits, PCR diagnostic kits, DNA extraction kits and RNA extraction kits. But what other applications besides these places? The following will introduce the applications of CPSA.

Brief introduction

3-(cyclohexylamine)-1-propanesulfonic acid is abbreviated as CAPS, which is a biological buffer. CAS1135-40-6, its molecular formula is C9H19NO3S, molecular weight is 221.32, it is white crystalline powder.

CAPS white crystalline powder

Buffer capacity of 3-(cyclohexylamine)-1-propanesulfonic acid buffer solution:
Adding a small amount of strong acid or alkali to the buffer solution will not change the pH value of the solution much, but if the amount of acid and alkali is large, the buffer solution will lose its buffering effect. This shows that its buffer capacity is limited.
The buffer capacity of the buffer solution is related to the concentration of the components that make up the buffer solution. The buffer solution composed of 0.1mol·L-1HAc and 0.1mol·L-1NaAc has a greater buffering capacity than the buffer solutions of 0.01mol·L-1HAc and 0.01mol·L-1NaAc. This point can be confirmed by calculation. But the concentration of the components of the buffer solution should not be too high, otherwise, the effect between ions cannot be ignored.
When the ratio of the two components of the buffer solution is not 1:1, the buffering effect is reduced and the buffering capacity is reduced. When c (salt)/c (acid) is 1:1, the △pH is the smallest and the buffering capacity is large. Whether for acid or alkali, it has a greater buffering effect. The pH value of the buffer solution can be calculated by the following formula:
At this time, the buffer capacity is large. The farther the ratio of the buffering components is from 1:1, the smaller the buffering capacity, and it may not even play a buffering role. For any buffer system, there is an effective buffer range, which is roughly within one pH unit on each side of pKaφ (or pKbφ).

1. CAPS is used as a biological buffer: CAPS is mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as a buffer for enzymatic chemistry and HPLC separation of alkaline drugs. CAPS can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at a pH of 10.5. It can also be dissolved in deionized water and adjusted to pH 11.0 for purification of fibronectin.
CAPS needs better purity when used as a biological buffer, and generally requires analytical purity. When used in industry, the purity requirements are relatively low, but different treatments must be made for different applications.
2. CAPS is used in industry: used for new coatings and new materials. CAPS is also the raw material for manufacturing welding materials, air conditioning equipment and manufacturing metal lithium.
3. Used as analytical reagent, heat exchange carrier, and also in the pharmaceutical industry. For air conditioning, it is also used to make fireworks, dry batteries and metal lithium, and also used as flux and desiccant.
4. For the coating industry, it is used as a water-based isocyanate curing agent. The water-based isocyanate curing agent can coexist stably with resins containing active groups (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time at room temperature.
The CAPS application is roughly that, the following will introduce the conventional configuration method of CAPS
Since the CAPS buffer is mainly composed of CAPS and deionized water, adjust the pH value with sodium hydroxide to avoid contact with air. Add deionized water to CAPS to 900ml, adjust the pH to 11.0 with 2mol/L NaOH, then dilute to 1L and store at 4ºC. Generally, a buffer with a high methanol concentration has a good effect on the transfer of low molecular weight proteins, while a buffer with a low methanol concentration or even no methanol is conducive to the transfer of high molecular weight proteins. The concentration of methanol in the CAPS electro-imprinting buffer is 0-20%, so the CAPS electro-imprinting buffer is more suitable for the transfer of low molecular weight proteins.

CAPS reagent

1. SDS-PAGE electrophoresis: similar to the nucleic acid hybridization method, except that the detected is the protein, the probe is the antibody, and the colored secondary antibody is labeled. Generally speaking, Tri-Glycerine or Tris-Tricine buffer system is used for low molecular weight proteins, and CAPS buffer system is better for high molecular weight proteins. Glycine in the buffer will affect sequencing, so the transfer buffer used for sequencing is 10mM/1CAPS10% methanol. The concentration of methanol in the CAPS electroimprinting buffer is 0-20%. Generally, a buffer with a high methanol concentration has a good transfer effect on low-molecular-weight proteins, while a buffer with a low methanol concentration or even no methanol is good for high-molecular-weight proteins. Transfer.

2. When PVDF membrane is processed, soak it in methanol for a few seconds, and then put it in CAPS electroimprinting buffer. Follow-up operations must prevent the membrane from drying out. If the PVDF membrane becomes dry, repeat the previous steps. The electrophoresis gel also needs to be soaked in CAPS buffer for 5-10 minutes. Some strongly basic proteins do not need this step.

3. The transfer conditions are usually 50V constant voltage, 100-170mA current intensity, and electroimprint transfer at room temperature. The bubbles between the gel and PVDF membrane must be removed. For proteins with higher molecular weight, pay attention to prolong the transfer time.

4. Before dyeing, PVDF membrane needs to be taken out and rinsed with deionized water, soaked in methanol, then dyed with 0.1% Coomassie brilliant blue for 30-50 seconds, decolorized with 50% methanol, washed with deionized water and dried.

The above introduces some matters needing attention in the protein imprinting experiment. In addition to the experiment itself, Desheng Technology reminds you that for safety and health, please wear laboratory clothes and wear disposable gloves for operation. The CAPS electro-imprinting buffer is recommended to be produced by Desheng The configuration of high-quality cyclohexylamine propanesulfonic acid.