CAS# 77-86-1 Good's Buffer TRIS Base Tris Hydroxymethyl Aminomethane Biological Buffer
All you want to know about TRIS buffer is here
1. What is TRIS buffer?
Tris(hydroxymethyl)aminomethane, or TRIS for short, is called tromethamine or THAM in medical applications. It is an organic compound with the molecular formula (HOCH2)3CNH2. It is widely used in biochemistry and molecular biology as a component of buffer solutions, and its derivatives include TAE and TBE buffers.
TRIS buffer powder
|PH value required(25℃)
|0.1mol/L HCI volume
2. What is the pH value of TRIS buffer?
Tris buffer is an ideal choice for most biological systems because its pKa value at 25°C is about 8.1 making it an effective buffer in the pH range of 7-9. This pH range is suitable for most biological experiments. This is also the reason why TRIS buffer is widely used.
3. Are Tris buffer and Tris base the same?
The pH value of Tris-base 1M is about 11, and the pH value can be reduced to 7 by adding HCL. But in Tris-Hcl, if you want to lower the pH to 7 you must add NaOH, because tris-Hcl contains hydrochloric acid, so it has a higher ionic strength (HCl + NaOH). But in Tris-base, you only need to add HCl.
4. Why use TRIS for DNA extraction?
Tris is a common biological buffer that can be used in the entire DNA extraction process. Because it is sensitive to pH during DNA extraction. Therefore, in the process of cell lysis, removal of unwanted cell components and precipitation, tris buffer is needed to maintain a stable pH value.
5. What is the difference between TAE and TBE?
TAE buffer is a solution composed of Tris base, acetic acid and EDTA. TBE buffer is a solution composed of Tris base, boric acid and EDTA. TAE has no effect on subsequent experiments, but the resolution is relatively low. The presence of boron ions in TBE will slightly affect the activity of some enzymes, but the resolution is high. Because TAE contains acetic acid, the buffering capacity is not as good as that of TBE.
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Advantages of Tris buffer:
① Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values ranging from acidic to alkaline;
② It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions.
Disadvantages of Tris buffer:
① The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1;
② The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution, △pKa/℃＝-0.031, for example: the pH of the buffer solution at 4℃=8.4, then the pH=7.4 at 37℃, so it must be Prepare at the temperature of use. Tris-HCl buffer prepared at room temperature cannot be used at 0℃～4℃;
③ It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
④ This buffer has a certain interference effect on some pH electrodes, so an electrode compatible with Tris solution should be used.
Tris buffer configuration method:
There are two ways to prepare Tris-HCl buffer: prepare 0.05 mol/L Tris and 0.05 mol/L HCl solutions respectively, and then mix according to the volumes listed in the common table. However, because standard concentration of dilute hydrochloric acid is not easy to prepare, another method is commonly used: Take the configuration of 1 L 0.1 mol/L Tris-HCl buffer as an example: first weigh 12.11 g Tris base and dissolve in 950 mL～970 mL deionized water , While stirring, add 4 N HCl dropwise, use a pH meter to measure the pH value of the solution to the desired pH value, and then add water to make up to 1 L.
Tris buffer pH range:
Tris buffer is a widely used buffer in biochemical research. Its commonly used effective pH range is in the "neutral" range, such as:
Tris-HCl buffer: pH=7.5～8.5
Tris-phosphate buffer: pH=5.0～9.0