Application of MOPS buffer in protein electrophoresis experiments

In the field of biological science, electrophoresis technology is a commonly used method for separating and identifying proteins. Among numerous electrophoresis techniques, MOPS buffer has gradually become an important role in protein electrophoresis due to its unique properties and advantages. MOPS is an organic compound containing 3-morpholinepropane sulfonic acid, which can effectively maintain the pH stability of the solution. In addition, MOPS also has a lower viscosity, which is conducive to the flow and separation of samples during the electrophoresis process.

Application in protein electrophoresis experiments

1. Stable pH value

In protein electrophoresis experiments, the electrophoresis effect is greatly affected by pH value. A stable pH environment helps to maintain the stability and activity of proteins during electrophoresis. MOPS buffer can effectively regulate and stabilize pH value, and is a commonly used buffer in protein electrophoresis experiments.

2. Improve resolution

The weak alkalinity of MOPS buffer solution gives it a high buffering ability, which can effectively resist ion exchange under the action of current. This enables the sample bands to better maintain their original shape and position during the electrophoresis process, improving the resolution of electrophoresis. Meanwhile, the lower viscosity of MOPS also helps to improve electrophoresis speed and reduce sample diffusion.

MOPS Powder

3. Protecting proteins

MOPS buffer can effectively protect proteins from the influence of acidic and alkaline environments while maintaining pH stability. This protective effect is beneficial for maintaining the natural state of proteins, making the electrophoresis results closer to the actual situation. In addition, MOPS can also stabilize the charge state of proteins through ion exchange, further improving the electrophoresis effect.

Advantages of MOPS buffer in protein electrophoresis experiments

1. High resolution: MOPS buffer can improve electrophoresis resolution while maintaining pH stability, which is beneficial for protein separation and identification.

2. Protein Protection: MOPS buffer has a protective effect on proteins, which can maintain their natural state and make the electrophoresis results closer to the actual situation.

3. Compatible with multiple detection methods: MOPS buffer has good compatibility and can be combined with multiple detection methods to provide more choices.

4. Low cost: Compared to other buffers, MOPS buffer has a moderate price and is suitable for large-scale protein research.

Product packaging

Precautions for use and storage

Preparation: When preparing the MOPS buffer required for protein electrophoresis experiments, it is necessary to prepare a suitable concentration of MOPS buffer according to the experimental requirements.

Usage: When preparing or using MOPS buffer, try to avoid getting on the skin or other organs as much as possible. If accidentally getting on the body and feeling uncomfortable, seek medical attention as soon as possible.

Storage: MOPS powder needs to be sealed and stored in a cool and dry environment to avoid exposure to air or sunlight; The configured MOPS buffer needs to be prepared and used immediately, and long-term storage is not recommended.

Waste disposal: Used MOPS buffer should be properly disposed of to avoid harm to the environment and human health.

Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer specializing in the research and production of MOPS and other biological buffering agents. Advanced production equipment and professional R&D personnel ensure product quality. The biological buffer produced by Desheng has high purity and few impurities; Stable production process with small inter batch differences; Good water solubility and affordable price. If you are interested in purchasing biological buffering agents recently, please feel free to inquire!

FAQ

Why do MOPS buffer for RNA?

MOPS buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane.

What are the properties of MOPS buffer?

MOPS Buffer or (3-(N-morpholino)propanesulfonic acid) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties.

What are the characteristics of MOPS buffer?

High water-solubility.

Low cell membrane permeability.

Consistent acid-base dissociation constants.

Low metal chelating capability.

High chemical stability.

Low absorption spectra in UV and visible regions.