China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Blood sample collection is an important part of modern medical diagnosis, and the selection of anticoagulants is crucial for ensuring sample quality, maintaining cell integrity, and ensuring accuracy in subsequent analysis. Among numerous anticoagulants, heparin lithium and EDTA (ethylenediaminetetraacetic acid) are the two commonly used, each with its own characteristics and suitable for different types of blood testing. This article aims to compare and study the application characteristics, advantages and disadvantages, and scope of application of lithium heparin tubes and EDTA tubes in blood sample collection, in order to provide reference for clinical practice and laboratory research. 1、 Anticoagulant mechanism and chemical properties Heparin lithium is a natural polysaccharide anticoagulant extracted from animal tissues. It mainly enhances the activity of antithrombin III, thereby inhibiting the activity of thrombin and other coagulation factors, achieving the goal of anticoagulation. Compared to other salt forms such as sodium and ammonium salts, heparin lithium has better water solubility and stability, making it more suitable for whole blood cell analysis and certain biochemical tests. Due to its ability to interact with various proteins, heparin may interfere with certain blood tests, especially those that rely on calcium ions for coagulation function testing. EDTA is a synthetic chelating agent that combines with calcium ions in the blood to form a stable complex, thereby preventing calcium ions from participating in the coagulation process and achieving anticoagulant effects. EDTA-K2 (potassium ethylenediaminetetraacetate) is a commonly used form of EDTA salt, suitable for routine hematological tests such as blood cell counting and classification, as it has a relatively small impact on the morphology and quantity of blood cells. However, the inhibitory effect of EDTA on platelet function limits its application in testing that requires evaluation of platelet function.   2、 Application scope and advantages Lithium heparin tubes are particularly suitable for blood gas analysis, hematocrit tests, and some biochemical tests due to their mild and stable anticoagulant effects. Heparin lithium can effectively maintain the original state of blood components, reducing possible cellular damage and metabolic changes during sample processing. Therefore, it is more advantageous for immediate detection (POCT) and testing items that require quick results in emergency situations. However, it should be noted that heparin has an aggregation effect on white blood cells and is not suitable for white blood cell counting and classification, and may interfere with the determination of specific enzyme activity. EDTA tubes are widely used in hematological tests, such as whole blood cell count (CBC), white blood cell classification, and red blood cell morphology, due to their ability to prevent blood clotting. The anticoagulant effect of EDTA is stable and reliable, and it has little impact on the morphology of blood cells. It is the standard anticoagulant for blood cell counting. However, the samples collected by EDTA tubes are not suitable for coagulation function testing, as the chelation of calcium ions can affect the determination of indicators such as prothrombin time (PT) and activated partial thromboplastin time (APTT). 3、 Comparison and selection When selecting anticoagulants, it is necessary to clarify the testing purpose. If the detection focuses on blood gas analysis, biochemical indicators (non calcium dependent), or needs to maintain the physiological activity of blood components, heparin lithium tubes are the preferred choice. When the focus of detection is on routine hematological indicators, especially when counting and classifying blood cells, EDTA tubes are more suitable. It is worth noting that for situations where both blood cell count and biochemical testing are required, separate sample collection or the use of dual anticoagulant tubes should be considered to avoid mutual interference between anticoagulants affecting the detection results. In summary, the applications of lithium heparin tubes and EDTA tubes in blood sample collection have their own advantages, and selecting appropriate anticoagulants is the key to ensuring the accuracy of test results. Clinical doctors and laboratory technicians need to fully understand the characteristics of various anticoagulants, select anticoagulant tubes reasonably based on specific testing needs, optimize sample processing flow, and improve testing quality and efficiency. In the future, with the development and application of new anticoagulants, blood sample collection technology will become more refined, providing more possibilities for medicine. As a raw material supplier in the biochemical market, Desheng provides high-quality heparin lithium, EDTA K2 anticoagulants, and other blood collection additives, all at a low price. If you have any relevant intentions, please click on the website to inquire about details and purchase!

In cell biology and molecular biology research, 293T cells, as a transformed human embryonic kidney cell line, have become a popular model system in scientific research due to their easy transfection and good expression of exogenous genes. To ensure that these cells can grow healthily and maintain their biological characteristics, accurate control of culture conditions is crucial, with pH stability being one of the foundations. HEPES buffer(4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) plays an irreplaceable role as a good buffer in maintaining pH stability in cell culture media. This article will delve into the appropriate pH range of HEPES and its application strategies in cell culture when cultivating 293T cells.   The role and importance of HEPES HEPES is an excellent organic buffering agent that can maintain pH stability of solutions over a wide pH range (usually 6.8 to 8.2). It does not interfere with most biochemical reactions and does not form stable complexes with metal ions, making it widely used in cell culture, enzyme activity research, and protein expression. For 293T cells, the addition of HEPES helps to reduce pH fluctuations caused by metabolic activity, ensuring that the cells are in a suitable growth environment. Ideal pH environment for 293T cells Although HEPES itself has a wide buffering capacity, the pH setting of the culture medium for 293T cells still needs to consider the physiological preferences of the cells. Research has shown that the 293 cell line (including 293T) prefers a slightly acidic environment, and pH values between 6.9 and 7.1 can promote cell adhesion and growth. However, in practical operation, considering the buffering effect of HEPES and the universality of cell culture media, it is generally recommended to adjust the pH value of the culture medium to between 7.0 and 7.2. This not only satisfies the preference of cells for a slightly acidic environment, but also ensures the effectiveness of the HEPES buffer system, avoiding cell growth inhibition or morphological abnormalities caused by alkalinity. Adjustment strategies in practice 1. The amount of HEPES added: According to the experimental requirements, adding an appropriate amount of HEPES can effectively buffer the acidic substances produced by cell metabolism during cell culture and maintain pH stability. 2. Using NaHCO3 to adjust pH: Although HEPES is the main buffering component, the addition of NaHCO3 to the culture medium is also necessary to preliminarily adjust the pH value to the target range. It should be noted that the purity and manufacturer differences of NaHCO3 from different batches may lead to slight changes in pH value. Therefore, when using a new batch of NaHCO3, a pH meter should be calibrated to ensure that the pH of the culture medium falls between 7-7.2. 3. Regular monitoring and adjustment: During the cell culture process, the pH value of the culture medium should be regularly monitored and fine tuned as needed. Although HEPES provides strong buffering ability, prolonged cell culture or high-density cell culture may cause pH to gradually shift. Timely replacement of fresh culture medium or appropriate addition of HEPES buffer is the key to maintaining a good culture state. 4. Personalized adjustment: The experimental conditions in different laboratories (such as carbon dioxide concentration, temperature, etc.) and the number of cell passages may affect the sensitivity of cells to pH, so it is essential to make personalized adjustments according to the actual situation while following basic guiding principles.   In summary, understanding and controlling the appropriate pH range of HEPES is a fundamental and important step in nurturing 293T cells. By properly allocating the use of HEPES and NaHCO3, combined with regular monitoring and adjustment, a growth environment close to the ideal state can be created for 293T cells, thereby improving the reliability of the experiment and reducing reproducibility. Remember, cell culture is both a science and an art, and subtle adjustments can often lead to significant improvements in experimental results. As an advantageous manufacturer of HEPES biological buffering agents, Desheng can supply raw materials with 99% purity for manufacturers to prepare and use, which is convenient, simple, and has stable buffering performance. If you have any relevant intentions, please feel free to inquire for details!

Blood gas analysis, as an important diagnostic tool in clinical medicine, has irreplaceable value in evaluating the respiratory function, acid-base balance, and electrolyte levels of patients. In this precise detection process, the selection of anticoagulants is crucial, and heparin lithium plays a core role in the field of blood gas analysis due to its advantages. This article will explore in detail the application and importance of heparin lithium in blood gas analysis from several aspects. Characteristics of heparin lithium Heparin lithium is a chemical substance with good chemical and physical properties. Its conductivity is low, the number of nuclear charges is small, and the number of electron layers is small, which makes it more stable and less likely to react with components in the blood. Secondly, the anticoagulant effect of heparin lithium is stable and not affected by temperature and pH value, which can ensure the accuracy of blood gas analysis. In addition, heparin lithium has a relatively small impact on calcium ion concentration, which can maintain stable calcium ion concentration in the blood and improve the reliability of blood gas detection.   The importance of blood gas analysis 1. Respiratory function assessment Blood gas analysis directly reflects the efficiency of pulmonary gas exchange. By monitoring partial pressure of oxygen (PaO2) and partial pressure of carbon dioxide (PaCO2), doctors can quickly determine whether patients have problems such as hypoxia or excessive ventilation, providing a basis for the diagnosis of respiratory failure and other diseases. 2. Acid base balance monitoring Blood gas analysis can also evaluate the acid-base balance of body fluids. It can help identify metabolic or respiratory acid-base imbalance through pH value, bicarbonate (HCO3 ^ -) concentration, residual base (BE) and other parameters, which is critical to the management of critical care, renal dysfunction, diabetes ketoacidosis and other conditions. The application of heparin lithium in blood gas analysis 1. Improve sample quality When collecting blood gas samples, adding lithium heparin as an anticoagulant can prevent platelet aggregation and red blood cell rupture, maintain the original state of the sample, and ensure the authenticity and repeatability of the test results. This is both the foundation and guarantee for blood gas analysis that requires high accuracy. 2. Promote real-time analysis Due to the excellent anticoagulant performance of heparin lithium, collected blood samples can be directly analyzed on the machine without waiting, greatly reducing diagnostic time and making it particularly crucial for emergency treatment of acute patients. This is particularly important in fast-paced environments such as ICUs and emergency departments. 3. Reduce operational complexity The use of heparin lithium pre charged blood collection vessels simplifies the pre-processing steps of blood gas analysis, reduces the operational burden and error rate of medical staff, and improves work efficiency and accuracy.     Advantages of heparin lithium in blood gas analysis 1. Reduce detection interference: Lithium heparin, as an excellent anticoagulant, is characterized by minimal interference with blood components. Compared to heparin sodium, heparin lithium has a smaller impact on the morphology and membrane integrity of red blood cells, significantly reducing the risk of hemolysis and ensuring the accuracy of blood gas analysis results. This low interference is particularly important for accurately measuring oxygen partial pressure, carbon dioxide partial pressure, and calculating other blood gas parameters. 2. Maintain sample stability: Blood gas analysis requires samples to be fresh and free of clots. Lithium heparin can quickly and continuously prevent the blood coagulation process, so that the collected blood samples maintain their biochemical characteristics unchanged before transportation and analysis. This feature is particularly crucial for quickly obtaining reliable diagnostic data in emergency situations. Conclusion In summary, the application of heparin lithium in blood gas analysis is not only based on its anticoagulant performance, but also on its ability to maintain sample stability. It serves as a bridge between clinical needs and laboratory testing, enabling rapid diagnosis of respiratory system and acid-base balance disorders. With the development of technology, the application of heparin lithium in blood gas analysis will become more widespread, contributing to improving the quality and efficiency of clinical diagnosis and treatment. Desheng Biochemical is a manufacturer specializing in the production of heparin sodium and heparin lithium for in vitro anticoagulants. Since its establishment in 2005, it has more than ten years of rich research and testing experience, and can effectively control the quality and efficacy of heparin. The manufacturer of the production source not only guarantees quality, but also has a very competitive price. Friends who need it can contact customer service on the Desheng official website for ordering!

In modern medical testing, blood analysis is an indispensable part of diagnosing diseases, and blood routine examination, as the basic blood testing item, its accuracy directly affects subsequent medical decisions. To ensure the reliability of the test results, blood samples need to be quickly processed after collection to prevent coagulation, which requires the use of anticoagulants. Among numerous anticoagulants, EDTA K2 (potassium ethylenediaminetetraacetate) is widely used in blood routine vacuum blood collection, rather than heparin or other types of anticoagulants, for scientific and specific reasons behind this.   The anticoagulant mechanism of EDTA K2 EDTA K2 is a calcium ion chelating agent, which means it can tightly bind with calcium ions in the blood to form stable complexes. Calcium ions play a crucial role in the process of blood coagulation, serving as a key cofactor in the activation of prothrombin to thrombin, which further catalyzes the conversion of fibrinogen to fibrin, forming blood clots. When EDTA K2 binds to calcium ions, it effectively reduces the concentration of free calcium ions, thereby hindering the activation of prothrombin and subsequent coagulation cascade reactions, achieving anticoagulant goals. Every 0.8mg of EDTA K2 can anticoagulate 1ml of blood, making it both economical and efficient to use. Reasons for choosing EDTA K2 instead of heparin 1. The impact on blood cell morphology is relatively small: Heparin, as another commonly used anticoagulant, although has good anticoagulant effects in many situations, may cause phenomena such as white blood cell aggregation and changes in red blood cell morphology, affecting the accuracy of blood cell counting, especially the impact on white blood cell counting and classification is more significant. EDTA K2 has a better protective effect on the morphology of blood cells, which can better maintain the natural state of cells and ensure the accuracy of blood routine test results. 2. The interference of EDTA K2 on detection items is small: EDTA K2 mainly affects detection items that rely on calcium ions, such as the determination of mineral content such as calcium and magnesium, while it hardly interferes with common detection items such as red blood cell count, white blood cell count, platelet count, and hemoglobin content in blood routine. In contrast, heparin may affect the determination of certain biochemical indicators, such as the results of certain enzyme activity measurements. Therefore, when multiple tests are required, EDTA K2 becomes a better anticoagulant. 3. Convenience of operation: Blood routine testing usually requires fast and accurate detection. The use of EDTA K2 is simple, and its vacuum blood collection vessel design can ensure accurate blood collection and instant mixing, reducing operational errors and improving detection efficiency. In summary, the reason why EDTA K2 has become an anticoagulant for blood routine vacuum blood collection is because it can effectively and specifically prevent blood coagulation, while having little impact on blood cell morphology and minimal interference with routine testing items, and has good safety and operational convenience. These characteristics together ensure the accuracy and efficiency of blood routine testing, providing reliable data support for clinical diagnosis. In the modern pursuit of accurate medicine, EDTA K2 plays an irreplaceable role in the field of blood routine testing due to its advantages. As a raw material supplier in the biochemical market, Desheng provides high-quality EDTA K2 anticoagulants and other blood collection additives, which are comprehensive and affordable. If you have any relevant intentions, please click on the website to inquire about details and purchase!

In the vast fields of biochemistry and cell biology, chemical reagents play a crucial role. They not only assist in the smooth progress of experiments, but also directly affect the accuracy and repeatability of experimental results. HEPES， The full name is 4-hydroxyethylpiperazine-1-ethanesulfonic acid, which is an indispensable chemical reagent that occupies a place in scientific research due to its buffering performance and wide applicability. Structure and properties HEPES is an organic compound with the molecular formula C8H18N2O4S, with a molecular weight of 238.3 Daltons. It belongs to the category of ionic zwitterionic buffering agents, which contain both acidic (carboxyl) and alkaline (amino) groups in the molecular structure. This characteristic enables HEPES to resist the interference of external acids and bases in certain pH ranges and maintain relative stability of the solution pH. HEPES forms a colorless and transparent solution after dissolution in water, with a pH range typically between 6.8 and 8.2. It is particularly suitable for simulating pH values under physiological conditions, making it an ideal buffer medium for cell culture, enzyme activity measurement, and various biochemical reactions. Application area 1. Cell culture In the field of cell culture, HEPES is a key component in maintaining pH stability of the culture medium. When cells are cultured in vitro, their metabolic activity produces acidic or alkaline substances, leading to pH fluctuations in the culture medium. HEPES can effectively buffer these changes, ensuring that cells grow in a pH environment close to physiological conditions, thereby improving cell health and proliferation. In addition, for cell lines that are intolerant to high concentrations of carbon dioxide, the HEPES buffer system can replace traditional bicarbonate buffer systems, reducing dependence on high CO2 incubators. 2. Biochemical experiments and reagent kits In biochemical experiments, HEPES is widely used for enzyme activity detection, protein purification, electrophoresis experiments, etc., as it can significantly improve the stability and repeatability of experiments. In clinical diagnostic test kits, HEPES, as a buffer, can ensure the stability of the reagents and the accuracy of detection results, especially in kits that require long-term storage or transportation, its buffering effect is particularly prominent. Safety and environmental protection considerations Although HEPES has broad application value in biochemical research, its use also requires attention to potential safety issues. High concentrations of HEPES may exhibit toxicity under certain cell culture conditions, affecting cell growth or causing changes in cell morphology. In summary, as an important biological buffer in biochemical research, HEPES has demonstrated its irreplaceable value in multiple fields such as cell culture and biochemical analysis. With the continuous progress of biotechnology and the increasing demand for environmental protection, research on the application efficiency and environmental impact assessment of HEPES will continue to deepen, promoting its wider and more efficient application in the field of biological science in the future. HEPES is not only a common chemical reagent in the laboratory, but also an important bridge connecting basic scientific research with clinical applications, theoretical exploration, and technological innovation. Hubei Xindesheng Material Technology Co., Ltd. was established in 2005 and is a leading manufacturer of HEPES buffering agents. It has a large-scale factory and a production research and development team. Professional personnel are available for pre-sales, during sales, and after-sales service, providing customers with full price protection throughout the entire process. The purity of the HEPES buffer currently produced can reach over 99%, with strict quality control. If you are interested, please feel free to inquire and purchase.

Luminol, also known as luminescent ammonia, is a chemical reagent that can emit light under specific conditions. In iron ion detection, its application is mainly based on its oxidation reaction and fluorescence emission phenomenon. Specifically, in alkaline media, luminol reacts with oxygen to produce an unstable intermediate product. When this intermediate product comes into contact with iron ions, it rapidly undergoes an oxidation reaction, generating excited state Luminol molecules. Excited Luminol molecules release energy upon returning to the ground state, which is emitted in the form of fluorescence. This article will elaborate on the principle, method, application advantages, and challenges faced by Luminol in detecting iron ions.   The principle of detecting iron ions with Luminol The principle of detecting iron ions with Luminol is mainly based on its specific reaction with iron ions in alkaline media. Under alkaline conditions, luminol reacts with oxygen to produce an unstable intermediate product. When this intermediate product comes into contact with iron ions, it rapidly undergoes an oxidation reaction, generating excited state luminol molecules. Excited Luminol molecules release energy upon returning to the ground state, which is emitted in the form of fluorescence.   Luminol detection method for iron ions 1. Sample preparation: It is necessary to prepare the industrial sample to be tested, ensuring that the sample does not contain any other substances that can interfere with the detection. For complex industrial samples, appropriate pretreatment may be necessary to remove impurities. 2. Preparation of Luminol solution: Dissolve an appropriate amount of Luminol powder in alkaline buffer to obtain a suitable concentration of Lumino solution. During the preparation process, excessive addition of luminol or alkaline substances should be avoided to avoid affecting the accuracy of the test results. 3. Mixing sample with luminol solution: Mix the test sample with luminol solution and measure the fluorescence signal intensity in the mixed solution using a fluorescence meter or other suitable instrument. 4. Data analysis: Measure the iron ions in the sample based on the intensity of the fluorescence signal. Standard curve method or other suitable data processing methods can be used for data analysis.   Application advantages of Luminol in detecting iron ions 1. High sensitivity: Lumino fluorescence reaction has high sensitivity and can detect extremely low concentrations of iron ions. This makes the method of trace monitoring of iron ions important in industrial production. 2. Good selectivity: Luminol fluorescence reaction has specific recognition ability for iron ions and can accurately detect the content of iron ions in complex samples. Compared with other metal ions, luminol has stronger selectivity towards iron ions, reducing the influence of interference factors. 3. Easy to operate: Luminol fluorescence method does not require complex sample pretreatment and instrument operation, and can complete the detection process in a relatively short time. This makes the method highly practical and convenient in industrial production. 4. Low cost: As a commonly used chemiluminescent reagent, luminol has a relatively low price and a simple preparation process. This makes the Luminol fluorescence method highly cost-effective in practical applications.   Matters needing attention 1. Sample processing: The sample to be tested may contain other metal ions, organic compounds, or impurities, which may react with luminol and interfere with the detection of iron ions. 2. Reaction conditions: Appropriate pH value, temperature, and reaction time are key factors affecting the luminescence effect. Therefore, before conducting the detection, it is necessary to carefully adjust these conditions to achieve better luminescence effects. 3. Reagent storage: Luminol reagents should be stored in a dry, cool, and dark environment to prevent them from becoming ineffective due to moisture, heat, or exposure to light. Luminol has broad application prospects and significant advantages in detecting iron ions in industry. By understanding its detection principle, methods, and precautions, use luminol for the detection and analysis of iron ions. As a manufacturer of chemiluminescent reagents such as Luminol, Desheng can supply analytical pure raw material powders. The solution preparation method is simple, and the sensitivity is high, with stable luminescence. It can assist you in quickly completing experiments in a short period of time. Due to the manufacturer's sale, the price is cheap. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!  

In medical laboratories, the collection and processing of serum is a crucial task. Serum, as an important component of blood, contains a large amount of biological information and has important guiding significance for the diagnosis and treatment of diseases and pains. However, in traditional serum collection methods, there are often problems such as low serum collection rates and cumbersome operations. In recent years, with the continuous development of separation gel technology, it has shown significant advantages in improving serum yield. This article will explore the principle and application of separation gel, as well as its impact on serum yield. The principle of separating adhesive Separation gel is a gel like substance with special physical and chemical properties that can form a stable isolation layer between serum and blood clots under the action of centrifugal force. This isolation layer can effectively separate serum from blood cell components such as red blood cells and white blood cells, thereby increasing the recovery rate of serum. The composition and properties of the separation gel determine its good stability and biocompatibility, without affecting the biochemical characteristics of the serum.   The application of separation gel in serum collection In medical laboratories, separation gel is usually added to blood collection vessels and used together with anticoagulants. When blood collection vessels containing separation gel and anticoagulants are used to collect blood samples, the separation gel rapidly forms an isolation layer between the serum and blood clots under the action of centrifugal force. This isolation layer can effectively block the entry of hemostatic cell components into the serum layer, thereby ensuring the purity and yield of the serum. Meanwhile, the separation gel can also protect the serum from external pollution and oxidation, maintaining its biological activity. The effect of separation gel on serum recovery rate Compared with traditional serum collection methods, separation gel technology can significantly improve the yield of serum. This is mainly due to the following aspects: 1. Good stability: The separation gel can quickly form a stable isolation layer under the action of centrifugal force, preventing blood cell components from entering the serum layer. This characteristic ensures the purity and yield of the serum. 2. Good biocompatibility: The separation gel has good biocompatibility and will not affect the biochemical characteristics of the serum. This enables the separation gel technology to be widely applied in various clinical tests and research. 3. Easy to operate: The blood collection tube using separation gel technology does not require complex pre-treatment operations, only the blood collection tube needs to be placed in a centrifuge for centrifugation. This feature simplifies the serum collection process and improves work efficiency. The advantages of separation adhesive technology In addition to improving serum yield, separation gel technology also has the following advantages: 1. Increased purity and stability of serum: Separation gel technology can prevent blood cell components from entering the serum layer, ensuring the purity and stability of serum. This makes the biological information in serum more accurate and reliable, which is beneficial for the diagnosis and treatment of diseases and pains. 2. Simplified clinical testing process: The use of separation gel technology for blood collection does not require complex preprocessing operations, simplifying the clinical testing process. At the same time, the separation gel can also protect the serum from external pollution and oxidation, prolonging the storage time of the serum. 3. Improved work efficiency: The separation gel technology simplifies the serum collection process and improves work efficiency. This is of great significance for the diagnosis and treatment of emergency patients. In summary, separation gel technology, as an advanced serum collection method, has shown significant advantages in improving serum yield. With the continuous development of medical technology, separation gel technology will play a more important role in clinical testing and research. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of blood vessel additives. Since its establishment in 2005, it has been engaged in the research and production of separation gels for 19 years with rich experience. And it has its own production plant and laboratory, and the indicators used are strictly formulated by R&D personnel according to experimental requirements. With high daily production and stable performance, it is a priority choice for domestic and foreign customers. If needed, please feel free to contact us.

In the field of hematological testing, blood type identification is a fundamental and crucial test, which has profound significance for blood transfusion, organ transplantation matching, and genetic research. Among the many available anticoagulants, ethylenediaminetetraacetic acid dipotassium salt (EDTA K2) is widely used as the standard anticoagulant for blood type identification, which contains profound scientific considerations and practical advantages. This article will explore why EDTA K2 has become an anticoagulant for blood type identification from multiple dimensions, including its anticoagulant mechanism, impact on blood cell morphology and blood type antigens, ease of operation, comparison with other anticoagulants, and its clinical and scientific applications.   1、 The anticoagulant mechanism of EDTA K2 EDTA K2 belongs to the salt of ethylenediaminetetraacetic acid (EDTA) and is known for its strong chelating ability, especially its ability to effectively bind calcium ions (Ca ²+) in the blood. Calcium ions play a core role in the process of blood coagulation, participating in key steps such as activation of prothrombin and conversion of fibrinogen to fibrin. EDTA K2 forms a stable complex with calcium ions, blocking the calcium dependent coagulation pathway and preventing blood clotting. This feature ensures that the blood sample remains liquid after collection, facilitating subsequent detection and analysis. 2、 Effects on blood cell morphology and blood type antigens Maintaining the natural morphology of blood cells and the integrity of blood type antigens is crucial in blood type identification. EDTA K2 has a relatively small impact on the integrity of the red blood cell membrane while chelating calcium ions, avoiding false positive or false negative results caused by changes in cell morphology. In addition, it does not affect the expression of blood type antigens, ensuring the accuracy of blood type identification. In contrast, certain anticoagulants such as heparin may affect platelet function and interfere with the recognition of blood type antigens, while sodium citrate may alter the volume and morphology of red blood cells, affecting blood type determination. 3、 Convenience of operation and sample stability A significant advantage of EDTA K2 lies in its ease of use. It can be attached to the inner wall of the blood collection vessel through the spray drying technology to ensure that the anticoagulant and blood are quickly and evenly mixed during the blood collection process, without additional operation steps. In addition, EDTA K2 anticoagulant blood samples can maintain stability for a long time under refrigeration conditions, facilitating transportation and storage, which is particularly important for cross regional blood type identification. This stability also allows the laboratory enough time to conduct detailed testing after receiving the sample, without worrying about the timeliness of the blood sample.   4、 Comparison with other anticoagulants Compared with commonly used heparin and sodium citrate, EDTA K2 has a more significant specificity advantage in blood type identification. Although heparin can effectively anticoagulate, it may interfere with the activity of certain enzymes, affect platelet function, and is not suitable for measuring platelet count and enzyme activity. Sodium citrate is mainly used for extracorporeal circulation and emergency anticoagulation. Its anticoagulation mechanism depends on the chelation of calcium ions and alkalization of blood, which may cause red blood cell swelling and affect the accuracy of blood type identification. EDTA K2 is relatively mild and has less interference with blood cells, making it more suitable for blood type identification, which is highly sensitive to cell morphology and antigen expression.   In summary, the reason why EDTA K2 has become the preferred choice for anticoagulants in blood type identification is because its anticoagulant mechanism can effectively prevent blood coagulation without damaging blood cell structure, maintain the true expression of blood type antigens, and demonstrate comprehensive advantages in ease of operation, sample stability, and comparison with other anticoagulants. These characteristics ensure the accuracy and reliability of blood type identification, and play an irreplaceable role in promoting relevant scientific research progress. Desheng stands out in the fiercely competitive market with its advantages and characteristics of high quality, strong research and development capabilities, and customized services. Choosing Desheng as your supplier of ethylenediaminetetraacetic acid dipotassium salt will provide you with high-quality products, professional services, and reliable guarantees. If you have any purchasing intentions, please click on the website to inquire about details!  

In the fields of chemistry and biochemistry, fluorescence labeling technology is a widely used tool that allows researchers to track and analyze biomolecules by detecting specific fluorescence signals. Among them, Acridinium Ester, as a commonly used fluorescent marker, has good chemical properties and is widely used in biological detection and imaging technology. The luminescence process of acridine ester involves the use of pre excitation solution and excitation solution, which play crucial roles in fluorescence labeling technology. This article will explore in detail their chemical principles and their applications in fluorescence detection. 1、 Pre excitation solution: hydrogen peroxide solution The pre excitation solution plays a crucial role in the luminescence process of acridine ester. Here, the pre excitation solution is usually hydrogen peroxide (H ₂ O ₂) solution. Hydrogen peroxide is a commonly used oxidant, which contains two oxygen atoms in its molecular structure. One oxygen atom forms a covalent bond with the other two hydrogen atoms, while the other oxygen atom exists in a single electron form, making hydrogen peroxide highly oxidizing. In the luminescence reaction of acridine ester, hydrogen peroxide acts as an oxidant and can react with the reducing groups in the acridine ester molecule, thereby triggering the generation of fluorescence signals. This reaction process is commonly referred to as "chemiluminescence" or "chemical excitation". In the reaction, the single electron in the hydrogen peroxide molecule undergoes electron transfer with the reducing group in the acridine ester molecule, causing the acridine ester molecule to transition from the ground state to the excited state. When the excited state of the acridine ester molecule returns to the ground state, energy is released and manifested in the form of photons, which is what we call a fluorescence signal. The concentration and purity of hydrogen peroxide solution have a significant impact on the effectiveness of fluorescence detection. An appropriate concentration can ensure sufficient oxidation, thereby effectively activating the acridine ester molecule; High purity hydrogen peroxide can reduce the interference of impurities on fluorescence signals, improve the sensitivity and accuracy of detection.   2、 Exciting solution: sodium hydroxide solution The excitation solution also plays an important role in the luminescence process of acridine esters. Here, the excitation solution is usually sodium hydroxide (NaOH) solution. Sodium hydroxide is a strong base with a molecular structure containing hydroxide ions (OH ⁻), which gives sodium hydroxide solutions a strong alkalinity. In the luminescence reaction of acridine ester, sodium hydroxide solution mainly plays a role in regulating the pH value of the reaction system. The intensity and stability of fluorescence signals are influenced by the pH value of the reaction system. Therefore, by adding an appropriate amount of sodium hydroxide solution, the pH value of the reaction system can be adjusted to be in the optimal range conducive to the generation of fluorescence signals. In addition, sodium hydroxide solution can also promote the reaction between hydrogen peroxide and acridine ester molecules. In alkaline environments, the oxidation of hydrogen peroxide is enhanced, making it easier to react with acridine ester molecules. This promoting effect helps to accelerate the generation of fluorescence signals and improve detection efficiency. 3、 The synergistic effect of pre excitation solution and excitation solution In practical applications, the synergistic effect of pre excitation solution (hydrogen peroxide solution) and excitation solution (sodium hydroxide solution) is the key to the successful chemiluminescence reaction of acridine ester. Hydrogen peroxide, as an oxidant, provides the energy required to excite acridone ester, promoting acridone ester molecules to enter the excited state. The alkaline environment created by sodium hydroxide not only optimizes this oxidation process, but also promotes the reaction between acridone ester and hydrogen peroxide, ensuring the efficiency and stability of the reaction. 1. pH regulation: Sodium hydroxide not only directly participates in promoting the oxidation reaction of hydrogen peroxide by adjusting the system pH to alkalinity, but also stabilizes the excited intermediate of acridine ester, helping to maintain an efficient luminescence process. An appropriate pH value can maximize luminescence efficiency while reducing the occurrence of side reactions, maintaining signal purity and intensity. 2. Reaction rate and efficiency: Under alkaline conditions, the oxidation ability of hydrogen peroxide is enhanced, and the reaction rate with acridine ester is accelerated, which shortens the induction period of the luminescence reaction, allowing the luminescence signal to be quickly generated and detected in a timely manner. This is particularly important for fields that require rapid detection results. 3. Signal strength and stability: By finely adjusting the ratio and concentration of pre excitation solution and excitation solution, the intensity and duration of the luminescence signal can be optimized, thereby improving the sensitivity and accuracy of detection. For example, a high concentration of hydrogen peroxide may cause the signal to quickly reach its peak and then rapidly decay, while a low concentration may not be sufficient to effectively excite luminescence; The concentration of sodium hydroxide needs to balance pH optimization and avoid potential damage to biological samples. 4. Application scope: Due to its direct chemiluminescence properties, acridine ester labeling technology combined with pre excitation and excitation solutions is widely used in clinical diagnosis, biological research, food safety testing and other fields such as immune analysis, nucleic acid hybridization detection, especially in real-time detection technologies that require high sensitivity and rapid detection. In summary, the appropriate ratio and use of pre excitation solution and excitation solution are crucial for achieving the optimization of acridine ester chemiluminescence reaction. Together, they ensure the efficiency, stability, and reliability of the luminescence reaction, providing powerful tools for scientific research and clinical detection. As an advantageous manufacturer of luminescent reagents, Desheng can provide stable luminescence and simple preparation. If you are interested, please click on the Desheng official website to learn more details!

 In the rapidly developing field of medical diagnostic technology today, the new Trinder's reagent MAOS has become an indispensable part of in vitro diagnostic reagents due to its chemical properties and performance. MAOS， N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt monohydrate has shown special value in clinical detection, disease monitoring, and research applications due to its significant advantages. Here are the six significant advantages of MAOS in the field of in vitro diagnostic reagents, and an in-depth analysis of how it promotes the advancement of diagnostic technology. 1、 Highly water-soluble: improving reaction efficiency and stability The highlight of MAOS lies in its water solubility, which makes its dispersion more uniform in aqueous media, significantly enhancing the stability and efficiency of the reaction system. Compared to traditional Trinder's reagents, the water solubility of MAOS reduces the precipitation and crystallization that may occur in the solution, ensuring the consistency and reliability of test results. This is particularly important for continuous automated testing processes, ensuring smooth operation of experiments and accuracy of data. 2、 High color sensitivity: achieving accurate detection of trace substances The molar absorbance of MAOS in color reactions is much higher than that of similar reagents, which means that even extremely low concentrations of analytes can be accurately detected. This is crucial for trace detection of biochemical indicators such as uric acid (UA), blood sugar, cholesterol, etc., providing the possibility for early detection and intervention of diseases. The high sensitivity of the in vitro diagnostic kit enables a more precise assessment of the patient's health status, providing doctors with more reliable diagnostic evidence. 3、 Strong stability: ensuring the long-term reliability of testing results MAOS exhibits good stability, which is a great blessing for in vitro diagnostic reagents that require long-term storage or use in different environments. Strong stability means that reagents are not easily degraded during transportation, storage, and use, maintaining consistency and repeatability of test results, and reducing the risk of misdiagnosis caused by reagent deterioration. 4、 Wide application range: adaptable to various detection needs MAOS is not only suitable for enzymatic photometric determination of hydrogen peroxide, but also serves as a companion for 4-AA coupled substrates, widely used for quantitative detection of various biomarkers such as uric acid, cholesterol, glucose, etc. This broad applicability provides more possibilities for the development of in vitro diagnostic reagents, meets different needs, and enhances the development of personalized medicine. 5、 Purity and quality control: ensuring the accuracy and reliability of data As a key component of in vitro diagnostic reagents, the purity of MAOS directly affects the accuracy of detection results. High quality MAOS products on the market are tested for purity using HPLC analysis method, ensuring a purity of over 99%, effectively removing impurities that may interfere with the detection, and ensuring the accuracy and credibility of each experiment. This strict quality control standard is the cornerstone of ensuring the reliability of diagnostic reagents. 6、 Innovation and Customization Potential: Promoting the Sustainable Development of Diagnostic Technology The emergence of MAOS not only brings upgrades to existing detection technologies, but also opens up new paths for future diagnostic reagent design. The particularity of its structure allows researchers to modify or functionalize it according to specific testing needs, and develop more efficient and specific diagnostic reagents. The potential for customization has profound implications for the diagnosis of emerging and rare diseases, as well as the development of medicine.     In summary, the application of MAOS in the field of in vitro diagnostic reagents is a microcosm of the progress of modern medical diagnostic technology. With high water solubility, high sensitivity, stable chemical properties, wide application range, strict quality control, and potential innovative customization capabilities, it has promoted the transformation of diagnostic reagents from traditional to convenient and efficient. With the deepening of research and technological progress, MAOS reagents and its derivatives will continue to play a crucial role in improving medical diagnosis and human health. Desheng is a manufacturer of new Trinder's reagents and can provide a series of reagents such as MAOS. Independently developed and produced, with small inter batch differences and sensitive reactions, it has important value in clinical diagnosis. If you need to make a purchase, please feel free to contact us for consultation and ordering!

In the research of biochemistry and cell biology, maintaining the pH stability of the experimental system is a crucial task, which is not only related to the accuracy and repeatability of experimental results, but also the fundamental guarantee for cell survival and normal physiological activities. In this context, HEPES (4-hydroxyethylpiperazine ethanesulfonic acid), as a non-ionic biological buffer, stands out among numerous buffer systems due to its unique chemical structure and physicochemical properties, becoming an indispensable "balance master" in scientific research.   The non-ionic properties of HEPES The reason why HEPES can occupy a place among many buffering agents is largely attributed to its non-ionic properties. As a zwitterionic molecule, HEPES contains both a weakly acidic carboxyl group (- COOH) and a weakly basic amino group (- NH2), which allows it to exist in varying degrees of dissociation in different pH environments, thereby achieving pH buffering. However, compared to some ionic buffering agents, HEPES mainly exists in an undissociated molecular form within the physiological pH range, i.e. a "non ionic" state, which gives it several special advantages: 1. Low interference: HEPES in a non ionic state hardly binds non-specific to biological molecules such as proteins and nucleic acids, greatly reducing potential interference with biochemical reactions, making it particularly suitable for biochemical analysis, enzymatic research, and cell culture that require strict experimental conditions. 2. High solubility and stability: HEPES can maintain good solubility even in high ion strength solutions, which means it can still play a buffering role well in complex biological systems. In addition, HEPES is not easily affected by temperature, light, and chemical substances, has strong stability, and is suitable for long-term experiments and storage. 3. Membrane permeability and selectivity: HEPES has membrane impermeability, which means its distribution inside and outside the cell does not significantly change the osmotic pressure of the cell, avoiding adverse effects on cell morphology and function. Meanwhile, this characteristic is particularly valuable in studying experiments such as transmembrane transport and ion channel function, as it allows scientists to more accurately control the extracellular pH without directly affecting the intracellular environment.   The application of non ionic properties of HEPES in multiple biomedical research fields 1. Guardian of cell culture: In cell culture, HEPES is the preferred buffer for maintaining the pH stability of the culture medium, especially in situations where operations outside the CO2 incubator, such as cell observation and transfection, are required. HEPES ensures that cells grow in a stable acid-base environment. 2. The ideal companion for biochemical experiments: In biochemical experiments such as enzyme activity determination, protein purification, DNA/RNA manipulation, etc., the low interference of HEPES ensures the conformation and activity of biomolecules, improving the accuracy and success rate of experiments. 3. Explorers of cutting-edge research: In fields such as protein structure analysis and cell signal transduction research, the non-ionic buffering ability of HEPES provides researchers with a stable and reliable experimental platform, driving the boundaries of life science to continuously expand.   HEPES， This seemingly simple chemical molecule plays a crucial role in the exploration of life sciences through its non-ionic buffering charm. It is not only an engineer who maintains the stability of the experimental system, but also an invisible wing that drives scientific progress. In the future, with the continuous innovation of biotechnology, the application scenarios of HEPES will further expand, continuing to use its subtle and powerful balance to safeguard every key step in unlocking the mysteries of life. In this pursuit of accuracy and harmony in the scientific symphony movement, HEPES is undoubtedly a beautiful melody that cannot be ignored. Hubei Xindesheng Material Technology Co., Ltd. was established in 2005 and is a leading manufacturer of HEPES buffering agents. It has a large-scale factory and a production research and development team. Professional personnel are available for pre-sales, during sales, and after-sales service, providing customers with full price protection throughout the entire process. The purity of the HEPES buffer currently produced can reach over 99%, with strict quality control. If you are interested, please feel free to inquire and purchase.

With the increasing awareness of environmental protection and the development of green chemistry, water-based curing agents have gradually replaced traditional solvent based curing agents and become important materials in modern industry due to their environmentally friendly, low volatile organic compound (VOC) emissions, and easy operation characteristics. CAPS (3-cyclohexylaminopropanesulfonic acid) buffer, as a compound with excellent performance, its application in water-based curing agents is particularly important. This article will discuss in detail why CAPS buffering agents can be used as water-based curing agents from the aspects of their characteristics, functions, and specific examples. 1、 Characteristics of CAPS buffer Good buffering performance: CAPS can provide a stable pH value in the neutral to alkaline range, with a pKa value of approximately 10.4. This feature enables CAPS to accurately adjust and control the acidity and alkalinity of the water-based curing agent reaction system, thereby optimizing the conditions for the generation of curing agents. High solubility: CAPS has good solubility in water, and can quickly dissolve and form a stable buffer when using deionized water. This allows CAPS to be easily mixed with water-based curing agents, reducing preparation steps. Temperature stability: CAPS has good temperature stability and can be used at higher temperatures without reducing its buffering performance. This characteristic allows the CAPS buffer to maintain its stability during the high-temperature curing process, ensuring the performance of the curing agent. 2、 The role of CAPS buffer in water-based curing agents CAPS buffer plays a crucial role in water-based curing agents, mainly reflected in the following aspects: Optimizing reaction conditions: By adjusting the acidity and alkalinity of the reaction system, CAPS buffer can reduce reaction temperature and pressure, reduce reaction time, and improve production efficiency. Meanwhile, it can also reduce the occurrence of side reactions and improve the purity and performance of the curing agent. Improving the performance of curing agents: The introduction of CAPS buffer can effectively improve the various properties of water-based curing agents. For example, by adjusting the crosslinking density and distribution of curing agent molecules, CAPS can improve the hardness, wear resistance, and weather resistance of the curing agent. In addition, it can also improve the drying speed and adhesion of the curing agent, making the coating smoother and smoother. Enhancing the stability of curing agents: CAPS buffer can stabilize the pH value of water-based curing agents, ensuring their stable performance during storage and use. This helps to extend the service life of the curing agent, reduce production costs, and improve its cost-effectiveness. 3、 The role of CAPS buffer in water-based curing agents CAPS buffer plays a crucial role in water-based curing agents, mainly reflected in the following aspects: Optimizing reaction conditions: By adjusting the acidity and alkalinity of the reaction system, CAPS buffer can reduce reaction temperature and pressure, reduce reaction time, and improve production efficiency. Meanwhile, it can also reduce the occurrence of side reactions and improve the purity and performance of the curing agent. Improving the performance of curing agents: The introduction of CAPS buffer can effectively improve the various properties of water-based curing agents. For example, by adjusting the crosslinking density and distribution of curing agent molecules, CAPS can improve the hardness, wear resistance, and weather resistance of the curing agent. In addition, it can also improve the drying speed and adhesion of the curing agent, making the coating smoother and smoother. Enhancing the stability of curing agents: CAPS buffer can stabilize the pH value of water-based curing agents, ensuring their stable performance during storage and use. This helps to extend the service life of the curing agent, reduce production costs, and improve its cost-effectiveness.   4、Summary and induction In summary, CAPS buffer plays an important role in water-based curing agents due to its excellent buffering performance, high solubility, temperature stability, and non toxicity. It can not only optimize reaction conditions and improve the performance of curing agents, but also enhance the stability of curing agents. With the increasingly strict environmental regulations and continuous technological progress, the application of CAPS buffer in the field of water-based curing agents will be more extensive. As an advantageous manufacturer of biological buffering agents such as CAPS, Desheng not only provides high-purity products, but also has stable supply and favorable prices. If you are interested, please feel free to contact us for purchase at any time!