China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The Application of HEPES Buffer       HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic buffer, CAS No. 7365-45-9, this buffer is generally used in biochemical experiments to adjust the PH of the reaction system, such as virus preservation solution or cell preservation solution. It has no toxic on cells,and maintain PH for a long time, It is always applied in cell culture medium and cosmetics. HEPES is a non-ionic amphoteric buffer with good buffering capacity in the pH range of 7.2-7.4. Its major feature is to maintain a relatively stable PH value in culture medium or cell observation. Under these standards, the caps of cell culture flasks should be tightened tightly to avoid required carbonate escaping into the air.   The way to prepare the HEPES buffer.     Hepes buffer can be added directly into the prepared culture medium with required concentration, followed by filter sterilization.Add 2.38 grams of HEPES powders to every 1000ml of culture solution, adjust the PH to 7.2 with 1NNaOH after dissolving, filter and sterilize before applying, at this time, the concentration of HEPES is 10mmol/L.     Adding 99ml cell culture medium solvent into 1ml stock solution,the concentration of HEPES is also 10mol/L.Below is the way to prepare HEPES stock solution with 1mol/L. First, dissolving 23.8g HEPES into 90ml double-distilled water;Second,adjusting the pH to 7.5-8.0 with 1N NaOH; Third,making up to 100ml with water titrant;Forth,filtering and sterilizing before packed into 2ml bottle;At last stored at 4℃ or -20℃. With high temperature resistant and melting point up to 200°C,HEPES powder won’t be degraded by autoclave. According to relevant literature, if the HEPES aqueous solution is exposed to ambient light for three hours, toxic hydrogen peroxide (H2O2) will be generated. Therefore,2 points need to be paid attention to,one is the HEPES aqueous solution must be kept away from light so as not to affect the experimental results. After being configured as a solvent, it should be stored at 4 °C and used in a short period of time for better results.Another is generally placed in a dry room and cannot be directly exposed to sunlight for a long time.         Hubei New Desheng Material Technology Co.,Ltd is specialized in producing biological Buffers over years.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises. For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

The Influence of EDTA-K2 pollution on heparin blood Biological testing   Disodium (EDTA-Na2), dipotassium (EDTA-K2) and tripotassium (EDTA-K3) are three kinds of Ethylenediaminetetraacetic acid (EDTA) salt，which commonly used as anticoagulant for testing.   In order to ensure that blood does not coagulate, blood collection tubes containing anticoagulant are widely used in normal Routine blood test. EDTA dipotassium (EDTA-K2) is always the best choice with better anticoagulant effect,and little influence on the shape of blood cells.   Because the solubility of potassium salt is better than EDTA-Na2,so EDTA-K2 and EDTA-K3 are more widely used than EDTA-Na2.Compared to EDTA-K3,EDTA-K2 can compensate for cell wrinkles caused by osmotic pressure with lower PH. EDTA-K2 pollution often causes abnormally high concentration of Potassium in patients, which is inconsistent with the clinical manifestations of patients.     However, EDTA-K2 pollution may also affect other biochemical indicators, but how much EDTA-K2 concentration effects, and what kind of conventional biochemical indicators affected ? This article by the lippi.g team in Biochem Med (Zagreb) systematically explores the effect of increased concentrations of EDTA-K2 on the results of routine biochemical assays.     Materials and methods: This paper recruited 15 volunteers from the laboratory staffs. Two vials of lithium heparin and one vial of EDTAK2 samples were collected from each study subject. EDTA blood sample was diluted with lithium heparin blood into different lithium heparin concentrations of 0%, 5%, 13%, 29% and 43% EDTA.Then,separating samples and testing biochemical indicators including ALT,bilirubin,cholesterol,creatinine,iron,LDH,lipase, potassium,sodium,chloride,magnesium and phosphorus.     Results indicate as EDTA2K concentration (from 5% concentration) increased, the amount of calcium, cholesterol, iron, lactate dehydrogenase, magnesium decreased,but potassium increased. The EDTA concentrations that significantly increased the results of the potassium were 13% and 29%. With the increase of the concentration of EDTA, the amount of ALT bilirubin and lipase did not change. Low concentrations of EDTA2K (from 5%) have greater effects on calcium, cholesterol, lactate dehydrogenase, magnesium and potassium. But for For sodium,phosphorus and iron,higher EDTA2K (from 29%) concentration needed.     Hubei Xindesheng Material Technology Co., Ltd. is a specialist of blood collection tube reagent manufacturers. The unit packaging volume of its anticoagulant EDTA dipotassium is 500g per bottle, and the shelf life is 3 years. It’s sold out to domestic and foreign customers. We have professional R&D team of blood collection tube anticoagulants with 17 years of experience. For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

How do you Choose the biological buffers for chromatography？     Chromatography is often used for the separation and purification of proteins in laboratory.In this process, the PH variations is related to the separation capability of the system. If the PH of solvent is near to pKa, for example, small changes in PH can strongly affect retention, and consequently the separation results.A buffer should be added to the system in order to control the mobile phase PH, Since the retention of ionizable compounds is especially sensitive to the PH variable.     According to relevant literature in chromatography,we found that although the Tris and MES are often the best choices for this technique, other buffers have also been used in cation exchange chromatography, anion exchange chromatography, high-performance liquid chromatography (HPLC) and other similar techniques.Below are the 10 best buffers for your reference. 1) BIS-TRIS buffer Suitable PH range: 5.8 - 7.2 PKA (25°C): 6.46 Molecular weight: 209.2g/mol Used as a buffer during anion exchange chromatography (Concerns: several components in a chromatographic system may be competing for metal binding with this buffer) 2) BES buffer Suitable PH range: 6.4 - 7.8 PKA (25°C): 7.09 Molecular weight: 213.2g/mol Used as a binding buffer and eluent in cation exchange chromatography / as a buffer in gel filtration chromatography 3) Bicine buffer Suitable PH range: 7.6 - 9.0 PKA (25°C): 8.26 Molecular weight: 163.2 g/mol Used as a mobile phase buffer and eluent in cation exchange chromatography 4) CAPS buffer Suitable PH range: 9.7 - 11.1 PKA (25°C): 10.40 Molecular weight: 221.32g/mol Used as a binding buffer and eluent in cation exchange chromatography Read more about Hopax CAPS 5) HEPES buffer Suitable PH range: 6.8 - 8.2 PKA (25°C): 7.48 Molecular weight: 238.3g/mol Used as a binding buffer and eluent in cation exchange chromatography in a two-stage reverse dialysis method for in vitro release testing 6) MES buffer Suitable PH range: 5.5 - 6.7 PKA (25°C): 6.10 Molecular weight: 195.2g/mol Used as a buffer in capillary electrochromatography,gel-filtration chromatography,phosphocellulose column chromatography,hydrophobic interaction chromatography,and cation exchange chromatography 7) MOPS buffer Suitable PH range: 6.5 - 7.9 PKA (25°C): 7.14 Molecular weight: 209.3g/mol Used as a running buffer for protein purification in chromatography 8) PIPES buffer Suitable PH range: 6.1 - 7.5 PKA (25°C): 6.76 Molecular weight: 302.37g/mol Used as a buffer in cation exchange chromatography,it should be used in lower concentrations due to its large ionic strength and dependence of concentration on pKa.And a buffer in phosphocellulose chromatography to purify microtubule proteins 9) TAPS buffer Suitable PH range: 7.7 - 9.1 PKA (25°C): 8.40 Molecular weight: 243.28g/mol Used as a buffer in planar chromatography to separate dyes,and in ion exchange chromatography for enzyme purification14 10) Tris buffer Suitable PH range: 7.5 - 9.0 PKA (25°C): 8.06 Molecular weight: 121.14g/mol Used as a buffer and eluent in anion exchange chromatography,and in capillary electrochromatography due to its low ionic mobility       Hubei New Desheng Material Technology Co.,Ltd is specialized in producing biological Buffers over years.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises. For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

About TRIS Buffer and TRIS-derived buffers     Buffer solution is usually a solution composed of weak acid and its conjugate base or weak base and its conjugate acid , which can change PH when a certain amount of other substances are added. The function of Buffer is to adjust the PH of the solution within a limited range so that the acidity of the solution to be tested conforms to the specified range by the analysis. In biochemical experiments and related research work, a large amount of buffer is required to maintain the PH of the testing system. Among them, TRIS and its derivative buffers are widely used in organic synthesis, coatings, fireproof materials, and biochemical and pharmaceutical fields. So, what are the advantages and disadvantages in practical applications? What is TRIS buffers?     Tris, also known as Tris (Hydroxymethyl) Aminomethane, Tris buffer is widely used, especially in nucleic acid and research experiments related to DNA, it has much more advantages than PBS phosphate buffer. Tris is also an important raw material for the synthesis of another biological buffer, TAPS.The advantages of Tris are very obvious. The PH range of tris buffer is 7-9, and the alkalinity is strong. Using this one buffer can configure PH buffers ranging from acid to alkali. At the same time, because it does not precipitate with metals,and with little interference with biochemical processes,tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many other important uses. With low ionic strength of Tris buffer ,it can be used for the formation of intermediate fibers of lamin in C. elegans.Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis.       There are disadvantages of TRIS buffer need to be noted:     First, the PH of TRIS buffer is greatly affected by concentration;     Second, the effect of TRIS buffer is changed by temperature,for example, at room temperature of 25°C, the PH of TRIS buffer is 7.8,but 8.4 when it’s reached at 4 °C and 7.4 at 37°C. Therefore, when the buffer is deployed at 4°C,but the test temperature is at 37°C, the results of its hydrogen ion concentration will be increase tenfold. At last, the buffering capacity is poor when the PH is lower than 7.5.   Several normal TRIS-derived buffers     TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservatives, etc.The PH of TBS buffer is 7.4. It is an isotonic buffered saline solution commonly used in biology, such as experiments in immunohistochemistry, situ hybridization, enzyme-linked immunosorbent assay. And washing non-specifically bound antibodies in western blot testing. TBS buffer is a salt buffer made by isotonic salt solution and TRIS-HCL buffer,mainly composed of TRIS-HCL, NaCl, tween20.The suitable PH range is 7.2-7.5.       TE buffer is a kind of electrophoresis buffer,prepared by TRIS and EDTA. The PH is 8.0. It is mainly used to dissolve nucleic acid, and store DNA and RNA stably.       TAE buffer is a buffer composed of Tris, acetic acid and EDTA. The PH range is 7.2-8.4. It’s a buffer widely used for short-term electrophoresis of large fragments of DNA.       TBE buffer is a solution composed of Tris base, boric acid and EDTA (ethylenediaminetetraacetic acid). TBE buffer is commonly applied in agarose gel electrophoresis to analyze DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning. Hubei Xindesheng Material Co.,ltd is a high-tech company specializing in the research and development, production and sales of biological buffers, blood collection tube additives, chemiluminescence reagents and luminescent substrates.       With seventeen years of rapid development, Desheng has a large R&D team and experimental base.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises.     For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

Application of Chromogenic Reagent,TOOS       3-(N-ethyl-m-toluidino)-2-hydroxypropane-sulfonic acid sodium salt (abbreviation is TOOS) is also named Sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate, which is a white powder with molecular formula of C12H19NO4S.Na and molecular weight is 331.36. TOOS is also called EHSPT, is often used in the following test kits: adenosine dehydrogenase detection kits for liver function testing, 5'-nucleotidase detection kits, glucose detection kits in blood glucose metabolism, glycation Albumin detection kit, 1,5-anhydroglucitol detection kit.TOOS with good characteristics water-solubility, high sensitivity and strong stability.       The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic assays and biochemical tests. They have several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity.The new Trinder's reagents are stable enough to be used with both in solution and experimental pipeline detection systems. With hydrogen peroxide and peroxidase, the new Trinder's reagent react with 4-aminoantipyrine (4-AA) or 3-Methylbenzothiazolesulfonehydrazone,and form a very stable purple or blue dye.The molar absorbance of dye formed by new trinders’ reagent reacting with MBTH is 1.5-2 times higher than with 4-AA; But MBTH solution is more stable. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of this hydrogen peroxide corresponds to the concentration of the substrate. Therefore, the amount of the substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol , acyl-CoA, and cholesterol can be used to detect those substrates coupled to the Trinder's reagents and 4-AA.TOOS is the most commonly used among 10 novel trinder’s reagent.However, it is necessary to develop more kinds of trinder’s reagent to match the specific substrates.         The chromogenic reagents produced by Desheng are impeccable in terms of purity, sensitivity, stability and appearance. The strict control of raw materials by quality inspection department from storage to production,guarantee the quality of chromogenic reagent.Only an enterprise that focuses on product research and development can provide customers with assurance. pls contact visit our website.http://www.whdsbio.cn/product/13.html

These Points About Tris Buffer You Should Know       Tris buffer is a zwitterionic buffer and one of the most commonly biological buffers applied in biotechnology research and manufacturing. In the pharmaceutical field, Tris buffer can be used for vaccines and antibiotics; in cosmetics, it is a buffer in the formulation of skin sunscreen products. In addition, Tris is also applied to prepare buffer solutions TAE and TBE and Laemmli.Tris can be dissolved in a variety of substances, including ethylene glycol and methanol. Below is the solubility of tris buffer in each solvent for your reference.   Solvent Solubility water 400 mg/mL Ethylene glycol 79.1 mg/mL Methanol 26 mg/mL 95% ethanol 22 mg/mL Acetone 20 mg/mL Anhydrous ethanol 14.6 mg/mL Dimethyl formamide 14 mg/mL Ethyl acetate 0.5 mg/mL Olive oil 0.4 mg/mL Cyclohexane 0.1 mg/mL Chloroform 0.05 mg/mL       Based on the characteristic and suitability for different application of biotechnological such as electrophoresis and chromatography,the main usage of tris buffer as follows: 1. commonly used in gel electrophoresis buffer solution TAE or TBE; 2. preparing Laemmli buffer; 3. making anion exchange chromatography; 4. evaluating bacterial endotoxin; 5. used in capillary electrochromatography (CEC) for its low ion mobility         Then what should be considered before choosing a TRIS buffer for research? First,the PH of buffer must be kept in 7-9; Second, tris buffer can get through cell membrane in specific condition, so it’s not suitable for many cells; Third,it is toxic to many mammalian cells; Forth,it is not suitable for use with bicinchoninic acid (BCA); Fifth,the temperature has big effect on the ph of tris buffer, so it’s quite important to prepare tris buffer solution under right temperature. Sixth,it reacts with various enzymes and inhibits their activity. Hubei New Desheng Material Co.,ltd is specialized in the production of tris buffer. For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

What is the Effect of Heparin in Making Sample of Blood-gas Analysis     Heparin is an anticoagulants naturally exists in all mammals, and named it when it’s isolated from liver tissue in 1916. Heparin is synthesized in mast cells and basophils and stored in the secretory granules of these cells. Since mast cells are present in many tissue cells, heparin can be derived from these kind of extrahepatic tissues. Currently, heparin preparations are often derived from the mucosal intima of pig intestines. Heparin prevents blood clotting due to the unique pentasaccharide sequence contained in its structure that binds tightly to antithrombin 3, which is a plasma protein that inhibits blood clotting by binding to several activated coagulation contents, such as XIa, Xa, IXa, and IIa (thrombin), which inhibit the activity of enzyme. Heparin blocks the fibrin formed by the coagulation cascade, which is required for blood clotting. This anticoagulant effect of heparin can occur in vitro and in vivo.     Heparin sodium is a natural heparin salt used in medicine and laboratories. Lithium heparin is a vitro anticoagulant used in laboratory, made from sodium heparin by cation exchange chromatography. Heparin is the only anticoagulant used to prepare samples for blood-gas analysis. There are two ways for heparin sodium to interfere results in analysis,one is the high heparin concentrations in the blood, and another is heparin can dilute the blood if liquid rather than dried (lyophilized) heparin is used. Traditional blood-gas analytes (such as PH, PCO2, and PO2) are less affected than electrolytes (especially ionized calcium), which are also measured by modern blood-gas analyzers. Therefore, if only PH, PCO2, and PO2 are measured, the requirements of blood samples are not too strict for heparin. For that analytes, it is still important that the concentration of heparin (sodium or lithium) is less than 200 IU/mL and the blood dilution can not exceed 5%.     One of the common practical problems related to blood-gas analysis is insufficient anticoagulation and formation of small blood clots, that can block the blood-gas analyzer and invalidate the results. The main reason is inadequate mixing of the sample with heparin. The lower the concentration of heparin, the greater the risk of insufficient anticoagulation with poor mixing techniques Hubei New Desheng Material Co.,ltd is specialized in making heparin sodium and heparin lithium which is used for anticoagulanting since 2005. With rich experience in researching and inspecting for 17 years, we can make sure the high quality and efficiency of heparin. The normal packages for our heparin are 10g/50g one bottle, and shelf life is 3 years. Pls contact us for high grade and competitive price for heparin. Our website is http://www.whdsbio.cn/product/13.html

Common Problems for Sodium Heparin in the Application of Blood Collection Tubes 1. Heparin sodium blood tubes are usually used in blood test, what kind of test suitable for?     Clinically, heparin sodium is mainly used for the prevention and treatment of thrombosis or embolic diseases, disseminated intravascular coagulation caused by various reasons,and hemodialysis, extracorporeal circulation, catheterization, microvascular surgery, etc. In the same time, it’s also used as anti-coagulant in blood testing and instrument. So the tubes with heparin sodium are used for anti-coagulation of blood. 2. How to use heparin sodium in tubes when doing research?     Usually put the heparin sodium on pipe, and dry it in oven within 10-300s.The time is based on volume of heparin sodium. 3. What is the purpose of drying? Can it be used directly without drying?     The purpose of drying is mainly to reduce the effect of heparin sodium on concentration of drug in plasma, and it can be used directly without drying. 4. Is heparin sodium resistant to high temperature?     Yes, sodium heparin is generally resistant to high temperature, and can be dried in an oven at 80 to 100 °C. It’s better to use the dried sodium heparin within one week. 5. Why is it best to use serum as the specimen when using sodium heparin to measure the content of protein?     When add heparin sodium in blood, it blocks the generation of thromboplastin and prevents blood coagulation, resulting in the inability of fibrinogen to be hydrolyzed and remains in plasma. When blood coagulated,the fibrinogen hydrolyzed into fibrin monomers and consumed,but the serum is separated out along with blood cells after centrifuging. In that case, the total protein content in plasma contains all proteins including fibrinogen, while the total protein content in serum does not contain fibrinogen, which should theoretically be lower than the total protein content in plasma. Therefore, it is best to use serum as the specimen when using sodium heparin to measure the content of protein.For some emergency specimens, or some patients who need to be measured urgently for other reasons,and plasma samples are needed to measure total protein, the content of fibrinogen should be deducted (generally 3% to 5%) after the total protein is measured. Only in this way can it be consistent with the total protein content measured by serum samples, and truly reflect the actual total protein content in the patient's body.       Hubei New Desheng Material Co.,ltd is specialized in the production of sodium heparin, lithium heparin products and other additives for blood collection tubes. For more information, pls visit our website.http://www.whdsbio.cn/product/13.html

The Series Of Virus Preservation Additives You Should Know     Hubei New Desheng Material Co.,ltd is specialized in making Viral Transport Media over years, and we developed several series as Normal Viral Transport Media,Inactivated and Non-inactivated viral transport media.   Products Name Characteristics Normal Viral TransportMedia 1.Color: Colorless or pink without guanidine salt 2.DNA and RNA of virus are not degraded under room temperature within 3 days. Inactivated virus preservation solution withguanidine salt 1.Without guanidine salt 2.DNA and RNA of virus are not degraded under room temperature within 3 days. Non-inactivated viral transport Media DNA and RNA of virus are not degraded under room temperature within 3 days.   Non-inactivated viral transport Media     To make sure the purification of water, the processes of multiple membrane purification, EDI purification,distillation, and membrane filtration are made by machine at one time, without the help of workers.In that case, there won’t be RMA enzyme and Bacterial endotoxin. It is good for storage of blood sample without degradation and keeping original feature. Inactivated viral transport Media     Inactivated viral transport Media is harmless,and inactivate respiratory pathogens instant.It used to prevent nucleic acid from degrading. Storage     Non-inactivated version and Inactivated one can be stored at 2-35 ℃ for 12 months. After sampling, it should be transported to the laboratory within 48 hours below 2-8°C, or stored below -70°C. And shelf life are one year. After the product is opened, the viral transport media can be stored at room temperature for 1 week. If you need to store it for a longer time, please store it at -80°C. Instructions 1. Clean your hands before sampling, and tear up the outer packaging of the sampling swab, then take out the swab. Please do not to touch the swab tip. 2. According to sampling requirements, use swabs to right part. 3. Put the swab into the sampling tube with preservation solution immediately after sampling,in case touching other parts. 4. Discard the extra parts and tighten the cap to complete the sampling. 5. Store the sampling tube properly, and deliver it to test within 1 week. 6. The specific sampling methods mainly include as following: Throat swab: Pass the swap over the base of the tongue, and wipe the bilateral pharyngeal tonsils and posterior pharyngeal. Nasal swab: Gently insert the swab into the nasal palate, stay for a while and then slowly take it out.     For more information ,please visit our website.http://www.whdsbio.cn/product/13.html

Influence of dipotassium EDTA pollution on the detection of heparin blood biochemical items Ethylenediaminetetraacetic acid (EDTA) is a commonly used anticoagulant for testing, there are three kinds of disodium (EDTA-Na2), dipotassium (EDTA-K2) and tripotassium (EDTA-K3). Routine blood is a common blood test item. In order to ensure that blood does not coagulate when blood is drawn, blood collection tubes containing anticoagulant are currently used. The commonly used anticoagulant is EDTA dipotassium (EDTA-K2). That is, its anticoagulant effect is very good, and it has little effect on the shape of blood cells. Because potassium salt is more soluble than sodium salt, EDTA-K2 and EDTA-K3 are better than EDTA-Na2; and because the pH of EDTA-K2 is lower than that of EDTA-K3, it can compensate for cell wrinkles caused by osmotic pressure. shrink, so come out. EDTA-K2 pollution often causes abnormally high K concentration in patients, which is inconsistent with the clinical manifestations of patients. However, EDTA-K2 pollution may also affect other biochemical indicators, but how much EDTA-K2 concentration affects which conventional biochemical indicators What about the impact? This article by the lippi.g team in Biochem Med (Zagreb) systematically explores the effect of increased concentrations of adherent EDTA-K2 on the results of routine biochemical assays. Materials and methods: This paper recruited 15 volunteers from the laboratory staff to be included in the study. Two vials of lithium heparin and one vial of EDTAK2 samples were collected from each study subject. EDTA whole blood was diluted with lithium heparin blood into samples containing lithium heparin at concentrations of 0%, 5%, 13%, 29% and 43% EDTA. Next, the samples were separated and tested for biochemical indicators: ALT, total bilirubin, total cholesterol, creatinine, iron, LDH, lipase, potassium, sodium, chloride, magnesium, phosphorus. Results: As EDTA2K concentration (from 5% concentration) increased, calcium, cholesterol, iron, lactate dehydrogenase, magnesium test results decreased, but potassium increased. The EDTA concentrations that significantly increased the results of the sodium phosphate assay were 13% and 29%, respectively. With the increase of the concentration of EDTA, the results of ALT, total bilirubin and lipase did not change. Low concentrations of EDTA2K (from 5%) have greater effects on calcium, cholesterol, lactate dehydrogenase, magnesium and potassium. For sodium phosphorus iron, it is necessary to have a high EDTA2K (from 29%) to have an effect. Hubei Xindesheng Material Technology Co., Ltd. is a relatively old brand of blood collection tube reagent manufacturers. The unit packaging volume of its anticoagulant EDTA dipotassium is 500g/bottle, and the shelf life is 3 years. It has served many domestic and foreign customers and has professional The R&D and production team of blood collection tube anticoagulants have accumulated 17 years of experience, rich product knowledge, stable product performance and good use effect. If you have any purchase needs, please feel free to call! For more product information ,please visit our website .http://www.whdsbio.cn/product/13.html

Carbomers usually come in the form of a fluffy, white, hygroscopic powder and may have a slight acetic acid odor. There are many types of carbomers available on the market, and the main uses are basically the same, the difference is the type of process solvent used ( benzene or non-benzene), the type and amount of crosslinking agent used, and the addition of optional additives to improve wetting and dispersion. Carbomer is a non-toxic substance with little or no potential to irritate skin and eyes at concentrations used in cosmetics and personal care products, so it is an important ingredient in cosmetics and pharmaceuticals. In its powder form, it needs to be used after preparation, and it is mainly used in these fields in the form of gel. Each carbomer particle is actually a macromolecule containing many linear PAA chains that are cross-linked together. After carbomer cross-linking, the result is that these macromolecules are not truly water-soluble. Instead, cross-linked hydrophilic PAA The mass of the chain is only water dispersible and water swellable. Unlike non-crosslinked PAA, which dissolves in solution to form polymer coils, overlapping and entanglement with increasing concentration, carbomers disperse in water and swell upon neutralization, forming a microgel solution. Our company ,Hubei Niew desheng Materials Technology Co., Ltd. is a professional manufacturer of carbomer. It has a professional carbomer R&D and production team with 17 years of experience and rich product knowledge. There are 940 and 980 models of Carbomer currently produced. The product has stable performance and good use effect. Free samples can be provided for a small test, if you need to buy, please feel free to inquire and buy! For more information about Carbomer ,please visit our website:http://www.whdsbio.cn/product/135.html

Foreign vacuum blood collection tubes were used during World War II as early as the 1930s, while my country is relatively backward. From the early 1990s, a very small number of companies began to develop and produce vacuum blood collection tubes. Subject to raw materials and technology, the development was relatively slow. . After more than ten years of development, my country's blood collection tube enterprises not only meet the domestic demand for blood collection tubes, but also export their products to foreign markets. The vacuum blood collection tube has the characteristics of accurate and safe blood collection, good effect of separating serum and plasma, easy to use, and can collect blood samples from multiple tubes with one needle. Therefore, we often see it when collecting blood in the hospital. Common blood collection tubes include PST tube, SST tube, EDTA tube, PRP tube, and CPT tube. What is the function of each? PST tube, the full name of Plasma Separator Tube in English, we often use it to represent the plasma separation rubber tube. Generally, a light green head cover tube is used. It combines serum separation gel and heparin sodium or heparin lithium anticoagulant to separate blood cells from serum and plasma to improve its output. , to ensure that the composition of plasma is stable, it is often used for electrolyte testing, and is often used for plasma biochemical testing in intensive care and emergency care in hospitals. SST tube, English full name Serum Separator Tube, inert separation and coagulation tube, generally used as yellow head cover tube. The coagulant in the tube can accelerate blood coagulation. After centrifugation, the serum separation gel in the tube can separate the liquid components (mainly serum and plasma) and solid components (mainly blood cells, proteins) in the blood, and form a stable in the tube. Barrier, sample specimens can be stored for 48 hours. This tube is mainly used for emergency serum biochemical and immunological detection. EDTA tubes, also known as EDTA anticoagulant tubes, generally use purple capped tubes. EDTA anticoagulants will use EDTA salts (dipotassium, tripotassium, disodium), because they have good chelation of calcium ions, thereby inhibiting the effect of thrombin to prevent blood coagulation. Since this coagulation is reversible, it needs to be detected within a certain period of time (generally no more than 24 hours). Compared with other anticoagulants, the EDTA salt has little effect on the coagulation of blood cells and the morphology of blood cells. It is generally used for blood routine testing, and is also suitable for the collection, transportation and storage of venous blood samples for nucleic acid testing. The serum separation gel in the tube can It can well block the interference of hemoglobin in red blood cells to nucleic acid detection experiments. PRP tube, English full name Platelet Rich Plasma, also known as PRP rapid extraction tube, is a high-end blood collection tube, which is often used in medical beauty projects. High-concentration plasma, and then inject it into the required parts, so as to achieve the effect of cosmetic repair and regeneration or treatment. CPT tube, full name in English, Cell Preparation Tube, vacuum mononuclear cell preparation tube, separates lymphocytes and mononuclear cells from whole blood through serum separation gel of different specific gravity, used in clinical medical testing, mainly to check HLA or residual leukemia genes Testing, TB testing, HIV testing, etc. Although different vacuum blood collection tubes are used in different fields, their purpose is to use the different specific gravity of serum separation gel to separate the blood components that need to be detected or extracted. At present, there are very few manufacturers on the market that support customized separation gels. Desheng Biochemical has continuously developed through 16 years of technical team. After four generations, the separation gel has the characteristics of anti-irradiation, good separation effect, stable quality and long storage time. Between 1.045-1.065g/cm3. Desheng separation glue can be divided into acrylate system and resin system according to different raw materials. The appearance includes transparent, translucent and opaque, and can be customized according to customer appearance requirements. The products produced are favored by more than 400 enterprises at home and abroad, and sell well in domestic and overseas markets. For more product information .please visit our website :http://www.whdsbio.cn/product/6/