China Wuhan Desheng Biochemical Technology Co., Ltd Company News

TRIS is a commonly used biological buffer. It is widely used in the field of biochemistry and is also an important chemical raw material. For example, common Tris-HCL, TAE, TES, Bis-Tris, etc. are synthesized from it. . This article will introduce the TRIS derivative TES and Bis-Tris in detail. 1. TES (Tris ethanesulfonic acid) TES Chinese name: Tris ethanesulfonic acid, CAS number: 7365-44-8, TES has a structure similar to Trizma, and is one of the ethanesulfonic acid series physiological buffers developed by Good et al. It meets various standards of "Good" buffers , although the structure is a neutral molecule, it still behaves as a zwitterion in solution. TES has a pKa of 7.4 (physiological pH), making it a very versatile biological buffer. TES is suitable for use in a variety of cell culture systems that require divalent metal ions, whereas many other buffers (such as citrate or phosphate) cause chelation or precipitation reactions that make this requirement unsatisfactory. In addition, TES is extremely beneficial to study the succinic acid oxidation reaction. 2. Bis-Tris (bis-trimethane hydrochloride) Bis-Tris full name in Chinese: Bis (2-hydroxyhexyl) amino (trimethylol) methane, CAS number: 6976-37-0, is an inert zwitterionic buffer, its solution varies with temperature There are only slight changes from the constant. Bis-Tris can ionize hydrogen ions in a small amount, has weak acidity, and is suitable for weakly acidic buffer systems. Due to the introduction of two hydroxyethyl groups in Tris, there are many atoms providing lone pairs of electrons, and it has complexing ability for some metal ions. EDTA or Bicine are similar. Bis-Tris is suitable for the separation of hemoglobin, and can protect the stability of hemoglobin during freeze-dried storage. Useful as an effective buffer for assays of muscle enzyme activity. 2-D gel electrophoresis is used as the catholyte in isoelectric focusing electrophoresis (IEF). Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of raw materials for diagnostic reagents. It can provide a variety of biological buffers, including Tris, Tris-HCl, Bis-Tris, Bicine, TAPS and other reagents. If you need to buy, please feel free to contact us ! For more information ,please visit our website:http://www.whdsbio.cn/product/8/

There are many different types of carbomer series products. Different carbomers have different performances, but the same generality. Appearance is loose white, slightly acidic powder, pH 2.5-3.0 (1% aqueous dispersion). Mainly used for short rheology, high viscosity, high clarity, low ion resistance and shear resistance, suitable for skin care lotions, creams, ethanol-containing transparent gel products, transparent skin care gels, etc. Here are 7 commonly used carbomer series products and their nuances in application and recommended dosage. Commonly used carbomer series Carbopol940: Recommended dosage: 0.2-1.0% Short rheology, high viscosity, high clarity, low ionic resistance and shear resistance, suitable for gel and skin care lotion, cream, transparent gel products containing alcohol, transparent skin care gel, hair styling gel gel, shampoo, shower gel Carbopol941: Recommended dosage: 0.1-1.5% Long rheology, low viscosity, high clarity, moderate ionic resistance and shear resistance, suitable for gels and lotions Skin care lotions, creams, clear skin care gels, hair styling gels (for medium ionic formulations) . Carbopol934: Recommended dosage: 0.2-1.0% Cross-linked polyacrylic resin, topical system, stable at high viscosity, for concentrated gels, emulsions, suspensions. Skin care lotions, creams, opaque gels, shampoos, body washes. Carbpol971: Recommended dosage: 0.2-1.0% Electrolyte-containing skin lotions, creams, ethanol-containing clear gels, clear skin care gels, hair styling gels, shampoos, baths. Carbopol974: Recommended dosage: 0.2-1.0% Oral products include gels, anti-allergies, whitening toothpastes and denture adhesives, clear skin lotions, creams, clear skin lotions, creams, gels, clear antiseptic gels with ethanol. Carbopol980: Recommended dosage: 0.2-1.0% Cross-linked polyacrylic resin, topical delivery system, crystal clear gel, water or alcohol solvent. Skin care lotion, cream, ethanol-containing transparent gel products, transparent skin care gel, hair styling gel, shampoo, shower gel. Carbopol1342: Recommended dosage: 0.2-1.0% Topical system, excellent rheology modifier in the presence of electrolytes, polymeric emulsification. There are so many commonly used series of Carbomer. The products most used by customers are Carbomer 940 and Carbomer 980, while the more common uses are hand-free gel, facial mask, shampoo, shower gel, skin care lotion, etc. Many customers have slightly different requirements for product transparency and viscosity. The carbomer produced by our Desheng company can be used in different products, and the customer response has always been very good. Desheng has been committed to providing high-service, high-quality products for the field of biomedical research, professional, efficient, safe, reliable, and high-speed direct access. The carbomer developed and produced has good gelation, viscosity, thickening, emulsifying, suspending and film-forming properties, and its chemical properties are stable and safe, without irritation and allergic reactions, and there are sufficient price concessions. Samples are available, welcome your inquiry!

Heparin is a class of naturally occurring anticoagulants present in all mammals, hence its name because it was first isolated from liver tissue in 1916. Heparin is synthesized in mast cells and basophils and stored in the secretory granules of these cells. Since mast cells are present in many tissue types, heparin can be derived from this range of extrahepatic tissues. Currently, heparin preparations are often derived from the mucosal intima of pig intestines. Heparin prevents blood clotting due to the unique pentasaccharide sequence contained in its structure that binds tightly to antithrombin 3. Antithrombin 3 is a plasma protein that inhibits blood clotting by binding to several activated coagulation factors, including factors such as XIa, Xa, IXa, and IIa (thrombin), which inhibit their enzymatic action. Heparin blocks the fibrin formed by the coagulation cascade, which is required for blood clot formation. This anticoagulant effect of heparin can occur in vitro and in vivo. Heparin sodium is a natural heparin salt used in medicine and laboratories. Lithium heparin is a laboratory-specific in vitro anticoagulant prepared from sodium heparin by cation exchange chromatography. Heparin is the only anticoagulant used to prepare samples for blood gas analysis. Heparin can interfere with the results in two ways. The first is high heparin concentrations in the blood, and the second is heparin thinning the blood if liquid rather than dried (lyophilized) heparin is used. Traditional blood gas analytes (pH, pCO2, and pO2) are less affected than electrolytes (especially ionized calcium), which are also measured by modern blood gas analyzers. Therefore, if only pH, pCO2, and pO2 are measured, the sample requirements are less stringent in the case of heparin. For these analytes, it is still important that the heparin (sodium or lithium) concentration is less than 200 IU/mL blood and the blood dilution does not exceed 5%. The electrolytes included in the test track preclude the use of sodium heparin and the use of lithium heparin. One of the common practical problems associated with blood gas analysis is insufficient anticoagulation and the formation of small blood clots that can block the sample path of the blood gas analyzer and invalidate the results. Inadequate mixing of the sample with heparin is often the problem. The lower the heparin concentration, the greater the risk of insufficient anticoagulation and related problems due to poor mixing techniques. Desheng Biochemical is a manufacturer specializing in the production of heparin sodium and heparin lithium for in vitro anticoagulants. Since its establishment in 2005, it has 17 years of rich R&D and inspection experience, and can accurately control the quality and potency of heparin. The conventional packaging of heparin produced by Desheng comes in 10g and 50g bottles, and the shelf life is 3 years. Since it is the source manufacturer, not only the quality is guaranteed, but the price is also very advantageous. Friends in need can contact customer service on the official website of Desheng to order.  For more information ,please visit our website http://www.whdsbio.cn/product/15.html

Carbomers are effective at increasing viscosity in aqueous systems at relatively low levels, with most carbomers capable of producing viscosities of 10,000–60,000 cP when used at only 0.5% w/w. Therefore, carbomers are commonly used as aqueous thickeners in various products. However, the real use of carbomer lies in its ability to impart high yield values ​​to formulations. Yield value is the resistance of a fluid to initial flow when stress is applied, and at rest, a tightly packed carbomer microgel network behaves as an elastic solid that resists deformation. The network does not begin to flow until a critical level of shear stress (i.e. yield stress) is applied, at which point the microgels can slide past each other, resulting in fluid flow. When heterogeneous phases, such as emulsion droplets, are dispersed in a carbomer-thickened formulation, the microgel network captures and stabilizes them against fluids at rest, ie, below the yield stress. However, when a stress in excess of the yield stress is applied, the fluid flows smoothly, enabling product dispensing and application. For this reason, carbomer is used to formulate countless products. Carbomer is easily dispersed in water and mixtures of polar organic solvents and water, such as 70% w/w ethanol-water. When initially prepared, aqueous dispersions of hydrated carbomer particles are acidic and typically exhibit a pH of 2.5-3.5, depending on the polymer concentration. These dispersions do not have significant viscosity and may be cloudy prior to neutralization with alkaline pH adjusters such as sodium hydroxide or triethanolamine. After neutralization of the carboxylate groups, the carbomer becomes ionized and expands to hundreds of its original volume due to electrostatic repulsion between the negatively charged carboxylate groups and osmotic expansion due to trapped counterions times to obtain a microgel dispersion. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of carbomer. It has a professional carbomer R&D and production team with 17 years of experience and rich product knowledge. There are 940 and 980 models of Carbomer currently produced. The product has stable performance and good use effect. Free samples can be provided for a small test, if you need to buy, please feel free to inquire and buy!

As the cumulative number of confirmed cases in China Jilin, Guangdong, Shandong and other places continues to increase, the nationwide nucleic acid detection has become the norm. The virus preservation solution is an important liquid medium added to the nucleic acid detection sampling tube. The commonly used virus preservation Liquids can be divided into VTM, UTM, MTM types. The standard UTM and VTM preservation solutions are designed to transport intact viable microbial or virus samples and culture them, so there are still biological hazards, which will be introduced in detail today. These three types of preservation solutions. MTM type virus preservation solution MTM is a molecular transport medium that provides users with a timely "snapshot" of that sample by preserving and stabilizing DNA and RNA. MTM (Molecular Transport Medium) is designed and optimized for molecular detection, allowing safe and efficient collection, transport and processing of pathogenic samples. Including qPCR and next-generation sequencing, it uses a unique patented technology to safely collect samples from patients with highly infectious diseases. It was first launched in 2006 in preparation for a global pandemic and has been used to detect many infectious and high-consequence pathogens, including influenza, RSV, tuberculosis, HIV and coronavirus. VTM and UTM type virus preservation solution In some foreign countries, both VTM and UTM refer to virus preservation solution. The full name of UTM is universal transport media, which is literally translated as universal transport medium. In virus sampling tubes, it refers to virus preservation solution. The VTM and UTM here usually refer to non-inactivated preservation solutions. Some manufacturers, VTM is more targeted than UTM, specifically for the preservation and transportation of virus samples. Although VTM or UTM cannot inactivate the virus, it can also be used for virus surface antigen or antibody detection in addition to nucleic acid detection because it preserves the integrity of the virus. In fact, the inactivated and non-inactivated virus preservation solutions do not say which one is better. Desheng can provide both kinds of preservation solutions according to the requirements of different manufacturers' products and testing environment. Advantages of MTM type virus preservation solution 1. Another major advantage of MTM is that samples can be safely stored for up to 7 days at ambient temperature or 28 days at 2 to 8°C and can be reused multiple times. In addition to reducing the risk of handling live pathogen samples, this also reduces costs by eliminating cold chain requirements and the need for Category 3 facilities as testing can be performed outside of the container 2. Once the swab or biological fluid is added to the MTM, the user gets a "snapshot" of the inactivated microbial sample in time. The microbial DNA, and more importantly, the labile RNA, is immediately stabilized and preserved at the sampling point. Therefore, samples in MTM do not have to be pre-treated to BLS-III or even BLS-II safety levels, only safe routine laboratory testing practices are required. These three virus preservation solutions have been introduced, and I believe that everyone's doubts can be answered. Desheng is a professional virus preservation solution manufacturer, and its quality has been recognized by many. Although the epidemic situation in many places is still severe recently, as long as everyone is united and united, I believe that we will soon see the dawn of the dawn, and everything will eventually return to the original beauty and tranquility.

http://www.whdsbio.cn/product/8/#c_product_list-15553982220823234-2HEPES and CHAPS buffers are both commonly used buffers in biochemical testing. The full chemical name of HEPES is 4-hydroxyethylpiperazine propanesulfonic acid, which is a zwitterionic buffer. It is a white powdery solid at room temperature. High water and water solubility. The chemical name of CHAPS is 3-[(3-chloroaminopropyl)dimethylammonium]-1-propanesulfonate, which is also a white powder and has good water solubility, but also hydrophobicity at the molecular level . When CHAPS is dissolved in water, the appearance of its aqueous solution will be a colorless to pale yellow transparent color. Application of HEPES buffer Since the growth and metabolism of cells in culture can cause dramatic changes in the environmental pH of the medium, it is necessary to add an appropriate amount of biological buffer to maintain an environment in which cells can be stably cultured. HEPES buffers in the pH 6.8-pH 8.2 range, which is beneficial for cell culture. So HEPES is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological and biochemical studies. Application of CHAPS Buffer Several studies have shown that, compared with NP-40 and Triton X-100, CHAPS can gently and effectively disrupt the nuclear membrane, solubilize membrane proteins, and disrupt protein-protein interactions (PPIs). In addition, CHAPS can preserve the native state or conformation of the protein, and can maintain the activity and function of the protein. Therefore, CHAPS is widely used in biological science research fields, such as cell lysis and protein purification. It is important to note that when preparing any solution using CHAPS, try not to shake or agitate vigorously. Desheng Company is a manufacturer engaged in the research, development and production of biological buffers. The HEPES and CHAPS products currently produced have stable performance, good use effect and a purity of 99%. If you need to buy, welcome to inquire and order

This article lets you understand the similarities and differences between MOPS and MOPSO MOPS (3-(N-morpholino)propanesulfonic acid) and MOPSO (3-(N-morpholinyl)-2-hydroxypropanesulfonic acid) in Good’s buffer It is two kinds of buffers that are widely used. From the English abbreviation, they only differ by one letter, so it is easy for the unfamiliar to confuse the two. In fact, there are both differences and similarities between MOPS and MOPSO. Next, I will briefly introduce their differences in application and configuration, as well as the common advantages of these two products. Differences between MOPS and MOPSO applications MOPS can be used in the following experiments: (1) As a running buffer in electrophoresis and for protein purification in chromatography; (2) It is formulated into a variety of agar media for the cultivation of bacteria, yeast and mammalian cells. However, concentrations higher than 20mM are not recommended for mammalian cells; (3) It can be used as a lysis buffer for E. coli cells; (4) as an eluent in gel filtration chromatography; (5) Applied to Northern hybridization as a buffer for RNA isolation and membrane transfer; (6) It is suitable for the determination of bisquinolinecarboxylic acid (BCA); (7) Electron transport and phosphorylation studies for chloroplast thin layer preparation. MOPSO can be used in the following experiments: (1) Used as a carrier electrolyte in capillary electrophoresis, and a crystallization buffer for glutathione synthase; (2) Used in fluorescence spectroscopy, spectrophotometry and isothermal titration calorimetry; (3) Interact with the peptide backbone of bovine serum albumin (BSA) to prevent thermal denaturation of BSA; (4) As one of the buffer components for copper-related analysis; (5) It is used as a component of buffered charcoal yeast extract medium; (6) Prepare MOPSO-ethanol buffer system to fix urine-derived cells; (7) It is used as a buffer system for the determination of by-products of biological treatment of marine crude oil. The difference between MOPS and MOPSO configuration How to configure MOPSO 1. Prepare 800 mL of distilled water (dH2O) in a beaker; 2. Put 112.63 grams of MOPSO powder into a beaker filled with distilled water (dH2O); 3. Adjust the solution in the beaker to the desired pH value with (10N NaOH); 4. Add distilled water (dH2O) until the volume is 1L and the configuration is complete. How to configure MOPS 1. Weigh 41.8g MOPS and place it in a 1L beaker; 2. Add about 700ml of DEPC treated water, stir to dissolve; 3. Use 2N NaOH to adjust the pH to 7.0, then add 20 ml of 1M NaOAc (DEPC treatment) and 20 ml of 0.5M EDTA (pH 8.0, DEPC treatment) to the solution; 4. Dilute the solution to 1L with DEPC-treated water; Filter with a 0.45um filter to remove impurities, and store at room temperature in the dark. Common advantages of Desheng MOPSO and MOPS: 1. The purity of Desheng MOPSO and MOPS both reach ≥99%, good water solubility, stable process, and can control a constant pH range for a long time. A full range of product testing and special testing services can be provided according to your application needs. 2. Desheng has a professional R&D and production team with a daily output of 1-2 tons and advanced production technology and equipment. There will be no long supply cycle or supply interruption. 3. For customers, we enjoy the advantage of trial packs. 4. The conventional packaging is 20KG/drum, but as a manufacturer, we can pack according to the needs of customers. 5. With strong logistics capabilities and rich export experience, we have exported to more than 100 countries and have established long-term cooperation intentions.http://www.whdsbio.cn/product/8/#c_product_list-15553982220823234-2

  Carbomer is a thickening agent that helps control the viscosity and flow of cosmetic products. They also help distribute and suspend insoluble solids into liquids and prevent separation of the oily and liquid portions of the solution. They have the ability to absorb and retain moisture and can expand up to 1000 times their original volume when dispersed in water. Typically, this type of ingredient is used in gel-like formulations because it forms a colloidal, mucus-like consistency when mixed with water. In cosmetic ingredient lists, the name carbomer is sometimes associated with numbers, such as 910, 934, 940, 941, and 934P. These numbers represent only the specific molecular weight and composition of the polymer. This ingredient is included in a variety of personal care products, such as styling gels, facial moisturizers, sunscreens, shampoos, and here are a few of the properties of carbomers. emulsification Additionally, carbomers are capable of dispersing or suspending insoluble solids in liquids. Therefore, carbomers are used to prevent separation of emulsions into oil and liquid components, as well as to control the consistency of cosmetic and other skin care products. By adding carbomer to products like shampoos, conditioners, creams and lotions, formulas will appear richer, smoother and creamier. texture By varying the amount of carbomer used in a product, formulators can create anything from a firm hairspray that stays exactly where it is placed, a lotion that has body but still flows easily, or a rich cream that retains its shape. Large amounts of carbomer in the gel may cause the product to roll or clump on the skin, but this phenomenon depends on other formulation steps taken to minimize this effect. skin benefits Interestingly, carbomer doesn't seem to have any effect on the skin. Once you apply carbomer on your skin and it dries, you won't even notice it. Carbomer does not make the skin sticky, dry or tight. Carbomer polymers are not in the product to promote any benefit, only to improve the texture of the product. Other uses In addition to being used in personal care products, carbomer polymers have a wide range of commercial and industrial uses. Some examples include use as fragrance carriers, medical bandages, wound dressings, and flame retardant gels, to name a few. Is carbomer safe? The safety of Carbomer has been assessed by the Cosmetic Ingredient Review Panel of Experts, which evaluates the safety of skin care and cosmetic products. Carbomer polymers exhibit low phototoxicity, light contact sensitization, skin irritation and sensitization at concentrations up to 100%. After evaluating the scientific data, a CIR panel of experts concluded that carbomer polymers are safe as ingredients in cosmetic and personal care products.  

EDTA dipotassium is an anticoagulant commonly added in clinical collection vessels, which is commonly used as blood routine in blood testing items. So does it apply to biochemical detection items? Today we are going to talk about common biochemical tests. Biochemical tests have many indicators, such as liver function test, renal function test, myocardial enzyme test, blood lipid and blood glucose test, etc. These tests seem to have little relationship with EDTA dipotassium, but they also add raw materials such as chromogens and enzyme preparations. EDTA dipotassium products are needed for hemoglobin and whole blood tests alone. In clinical biochemical examination items, if EDTA dipotassium blood collection anticoagulation tube is used for biochemical examination, high potassium, low calcium, low magnesium, low ALP and other effects will occur. Why do you say that? They are listed as follows: 1. EDTA dipotassium itself contains potassium ions, resulting in high blood potassium. 2. EDTA can chelate calcium ions in blood samples resulting in low calcium. 3. EDTA chelates magnesium ions in blood samples, resulting in a decrease in blood magnesium. Note: In addition to calcium ions and magnesium ions, EDTA also chelates other divalent metal ions, such as manganese ions, iron ions and so on. 4. Magnesium ion is the activator of ALP activation center, so EDTA dipotassium also reduces ALP activity. In conclusion, due to the infiltration and dilution effects of EDTA dipotassium, it may have an uncertain impact on the data accuracy of biochemical examination items. In order to avoid these effects, correct anticoagulants should be selected in different tests. Desheng Biochemical develops and manufactures various vascularized anticoagulants, including heparin, EDTA potassium sodium salt, potassium oxalate, trisodium citrate, etc. If necessary, please contact Desheng, and the sales manager will recommend appropriate anticoagulants to you according to your needs.  

There are two kinds of blood collection additives: coagulant and anticoagulant, among which heparin series and EDTA series are widely used in the market. In addition to these anticoagulants, there is also an anticoagulant reagent "potassium oxalate". How many people know the anticoagulant function principle of potassium oxalate? The following will lead you to know potassium oxalate.   Potassium oxalate as an anticoagulant has the advantage of high solubility, and its action mechanism is easy to understand. The dissolved oxalic acid reacts with calcium ions in the blood to form calcium oxalate precipitation. Therefore, the coagulation function of calcium ion itself is invalid, which prevents the coagulation process of calcium ion. The concentration of potassium oxalate anticoagulant is usually mixed with blood in the ratio of 1:9, and the concentration is 0.1mol/l. Not too high concentration of potassium ion can easily lead to dehydration and contraction of blood cells. Therefore, in order not to affect the volume and morphology of red blood cells, the mixing proportion of anticoagulants must be quite appropriate when measuring hematocrit.   Although potassium oxalate is used in many aspects of blood biochemical determination, some determinations are not appropriate. For example, the determination of potassium and calcium ions, blood smear examination, etc. In addition, potassium oxalate cannot be used for differential counting of platelets in leukocytes and blood cells, because potassium oxalate can cause platelet aggregation and affect the basic morphology of leukocytes.   It can be concluded that potassium oxalate, as a popular product in anticoagulant, is affected by these specific factors. At present, there are few manufacturers of potassium oxalate in China, and our company is a professional manufacturer of blood collection additives. At present, the company's blood collection additives include heparin potassium, heparin lithium, EDTA dipotassium / sodium, EDTA Tripotassium, coagulant, high-efficiency coagulant powder, water-soluble silicide, oil silicon, potassium oxalate, trisodium citrate, sodium fluoride and other products. In addition to exploiting vascular additives, our company also produces biological buffer series, chemiluminescence reagent series, chromogen substrate series, enzyme preparation series, Carbomer and virus preservation solution.   Hubei xindesheng Material Co., Ltd., as a professional manufacturer of blood collection additives, has decades of R & D and production experience. It has rich professional knowledge and ability in blood collection additives and provides products for many enterprises in the world. If you are willing to cooperate with our products, you can contact relevant personnel on our official website for consultation. Desheng welcomes your visit.

Enzyme is an active substance existing in nature. These substances have a very far-reaching impact on our life and health. Enzyme manufacturers can prepare various types of enzymes in different ways to meet different production needs, such as the needs of biochemical detection, food processing and so on. So how do these manufacturers choose cost-effective enzyme manufacturers?   1. Select manufacturers with rich varieties There are many kinds of enzymes in nature, each of which can play different roles. When used in production, sometimes a variety of enzymes may need to be added due to the needs of process technology. Therefore, in order to achieve one-stop procurement, manufacturers should try to choose enzyme manufacturers with a wide variety of enzyme preparations, so as to ensure that the purchased enzyme preparations have the same quality, Desheng has more than ten years of experience in the production and R & D of enzyme preparations. There are dozens of enzyme products, which can meet the needs of various production enterprises. 2. Select manufacturers with excellent raw material quality The production of various enzyme preparations needs to use different molds for fermentation. In this process, whether the quality of raw materials is excellent or not can determine the quality of enzyme preparations. In order to ensure the purchase of the best enzyme preparations, the manufacturer should choose the enzyme preparation manufacturer with high requirements for the quality of raw materials, which can not only maintain the reliable quality of enzyme preparations, At the same time, it can also ensure that the product quality processed by the enterprise is better. Desheng's enzyme preparation is specially used in biochemical detection and biochemical experiment, which has better purity and activity than industrial enzyme preparation.   3. Select manufacturers with good technology Although most enzyme preparations are refined after fermentation, with the continuous development of science, technology and industry, the processes of various enzyme preparations are also improving. The use effect of enzyme products produced by enzyme preparation manufacturers with better processes will be better. Therefore, the production process should also be considered when selecting enzyme preparation manufacturers.   The above three points are the methods for Desheng Xiaobian to select enzyme preparation manufacturers. No matter what type of enzyme preparation the enterprise needs or the purpose of using enzyme preparation, it is very important to select an enzyme preparation manufacturer with high cost performance in order to purchase high-quality and effective products. Therefore, the purchaser should have a comprehensive understanding of the enterprise, Select the best manufacturer after comprehensive comparison.

Chemiluminescence immunoassay is not only the fastest developing and popularizing immunoassay method, but also the most advanced labeled immunoassay technology. Its sensitivity and accuracy are several orders of magnitude higher than enzyme immunoassay and fluorescence method. It can completely replace radioimmunoassay and completely eliminate enzyme-linked immunoassay. It mainly has the advantages of high sensitivity, strong specificity, low reagent price, stable reagent and long validity period (6-18 months), stable and rapid method, wide detection range, simple operation and high degree of automation.   The chemiluminescent reagent used as a marker shall meet the following conditions: Luminol and acridine ester luminescent agents are commonly used labeling luminescent agents, which meet the following conditions: 1. Can participate in chemiluminescence reaction. 2. It can form a stable conjugate reagent after coupling with antigen or antibody. 3. After coupling, high quantum effect and reaction kinetics are still retained. 4. The physicochemical properties, especially immune activity, of the labeled substances should not be changed or rarely changed. Classification of markers used in chemiluminescence immunoassay 1 Direct chemiluminescent agent Direct chemiluminescent agent refers to the direct labeling of antigen or antibody with chemiluminescent agent, which mainly includes the following types.   ① When acridine ester is oxidized by H2O2 under alkaline conditions, it emits light with a wavelength of 470nm and has high luminous efficiency. Its excited state product N-methyl acridone is the illuminant of the luminous reaction system. Acridine ester luminescent agents can be directly used to label haptens and proteins. When used to label antibodies, the binding is stable, the production of light quanta is not reduced after binding, and high specific activity can be obtained, which is conducive to the establishment of two site chemiluminescence immunoassay. Acridine ester is an ideal luminescent substrate.   ②Tripyridine ruthenium tripyridine ruthenium is an electrochemiluminescence agent. It and electron donor tripropylamine (TPA) can lose an electron on the surface of the anode electrode at the same time.   2 Enzymatic reaction luminescent agent: the luminescent agent (substrate) emits light by the catalysis of labeled enzymes (horseradish peroxidase and alkaline phosphatase).   ①The luminescent agent catalyzed by horseradish peroxidase is luminol and its derivatives; In addition, isoluminol and its derivatives have chemiluminescence properties. Luminol is the first synthesized luminescent substance.   ②The luminescent substrate catalyzed by alkaline phosphatase is amppd, amppd It is a new chemiluminescent agent. There are two important parts in its molecular structure. One is the dioxygen ring connecting benzene ring and adamantane, which can break and emit photons; The other is the phosphate group, which maintains the stability of the whole molecular structure.