China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Carbomer is a white, loose powder with strong hygroscopic properties, soluble in water, ethanol and glycerin. It can be used as topical lotion, gel, cream. The overall carbomer gel system of neutral Ph under 7 environment is an excellent gel matrix, with crystal clear appearance, smooth and moisturizing touch, suitable for cream or gel preparation. At the same time, due to its simple addition process, it is easy to use. It feels comfortable afterwards, and is used in partial administration, especially in skin and eye gels. It has a wide range of applications. Although it can be combined with water to form a gel-like transparent milky alternation, it is not directly soluble in water like traditional inorganic salts. Today, we will introduce an alkaline preparation method to prepare an ideal carbomer gel solution. .   First weigh 1g of Carbomer 940 dry powder in a 50ml dry beaker. If you encounter agglomerates, please use a spoon to crush it. Then add 25ml of purified water, stir while adding, so that Carbomer 940 fully absorbs water and swells. Then you have to let the Carbomer 940 fully absorb water. Generally, after adding water and stirring, leave it for a few hours before the Carbomer 940 can fully swell. If you want to go faster, you can heat it in a water bath. While heating and stirring, it will form faster. Even slow heating can accelerate the water absorption and swelling process of Carbomer 940, and it takes 10 to 30 minutes. . Then use a dropper to add the lye, stirring while adding, and measuring the pH value. When the pH is adjusted to 7, the gel is well prepared. (Triethanolamine can be used for lye). At the same time make up the remaining amount of water. The total volume is maintained at 50ML. The formulated gel base can be directly or indirectly mixed with other raw materials to prepare various gels, as long as the carbo concentration is controlled to be no more than 0.8%, and it is more suitable to be about 0.5%. Only 0.5% ingredients are needed to make a gel product with good appearance and texture. It is like making a 100ml gel. Take 25g of a pre-prepared 2% polymer gel and add it to a 75ml formula. 100ml of 2% transparent gel contains the following components, decomposed as follows: 2gm gel powder + 98ml pure water + 0.5ml antibacterial agent + appropriate amount of neutralizer. DIY Carbomer 940 gel.   Need special reminder: Don't add anything indiscriminately! Such as soluble salts, very acidic solutions (lemonade, vinegar). The carbomer series 940 gel system becomes thin immediately. Although hyaluronic acid is also acidic, the added amount is small, which has little effect on the stability of the carbomer 940 gel system, so feel free to add it. Pure dew is slightly acidic, and the effect is not significant after adding.   Carbomer 940 itself has no nutrition, does not support the growth of bacteria and molds, but does not prevent bacteria and molds, and uses the nutrients present in the gel system to promote their growth. So don't mix too much at one time, add preservatives or essential oils as needed. )Ultraviolet rays have a certain effect on the Carbomer 940 gel system, so please keep it away from light. When formulating skin care products, it is appropriate to control the final concentration of Carbomer 940 not to exceed 0.8%. It is recommended that the concentration be around 0.5%. If the concentration exceeds 0.8%, it is easy to form film. Personally, I think the recommendation is about 0.7 is more beautiful, and the concentration is too thin, which belongs to another category-a thicker essence.   Desheng Biochemical was founded in 2005 and has been specialized in the R&D, production and sales of Carbomer 940/980 for many years. On the basis of many years of practice, independent intellectual property rights and professional R&D capabilities have been formed and produced. Provide products and raw material solutions for domestic and foreign daily-use washing and cosmetics manufacturers.

When it comes to carbomer, most people don't talk about it often, but carbomer is used in many cosmetics. Even though they may benefit from carbomer every day, most people don't know what carbomer is. Carbomer is not like an active ingredient that provides a result-oriented product, but an inert ingredient that can help these active ingredients play an outstanding role. Carbomer describes a series of polymers made of acrylic acid. It is a white fluffy powder, but it is often used as a gel in various cosmetics and personal care products. These cosmetics and personal care products are used in skin, hair, nails and makeup products and dentifrice. Carbomer is also commonly listed as Carbomer 934, Carbomer 934P, Carbomer 941, Carbomer 910 and Carbomer 910. Thicken or homogenize the consistency of the product Carbomer is a thickening agent that helps control the consistency or viscosity and fluidity of many cosmetics. For the hands that need to be disinfected during the journey, the natural hand sanitizer gel is 99.9% effective in fighting common bacteria, and its formula will not make your hands feel sticky or dry.   Prevent product separation In addition, they help to disperse and suspend insoluble solids in liquids and prevent oil and liquid parts from separating in the solution. This useful property is what makes thinner moisturizers such as emulsions work.   Improve the texture of the product Carbomer can easily absorb and retain water, and can greatly expand when suspended in water, up to 1000 times the original volume. By adding carbomer to shampoo, conditioner, cream and lotion, these The formula will appear richer, smoother and creamy.   Delay aging and reduce skin cracking Peptide eye gel, due to the presence of carbomer, it provides the moisturizing properties of squalane and the anti-aging effect of peptides in the gel formulation. Use a gel-like consistency. Use Aloe Vera in Gel Aloe 95 to help moisturize your skin. Aloe vera has many skin healing properties, such as reducing inflammatory acne, preventing fine lines and wrinkles, and when dandruff is used on the scalp, it can also Help solve hair problems such as dandruff.   Desheng Biochemical is a manufacturer specializing in carbomer, supplying carbomer 940/980 all year round. Welcome to consult

Accurately locate the single product of biological buffer you need   Biological buffers have a very wide range of applications in the field of biochemical engineering, but we don't know much about it. Now we Desheng take you to understand the biological buffers we produce: Tris, BICINE, HEPES, CAPS, MOPS, etc. are used where and where are their advantages, so that you can clearly position when buying products. To the product you need, and help you get a general understanding of the product.     NO.1 tris (Tris(hydroxymethylaminomethane) or tromethamine) Used in the preparation of buffers in biochemistry and molecular biology experiments; pharmaceutical intermediates; used in the preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as coating dispersants; indoor formaldehyde adsorption Preparation of materials, etc.     NO.2 Bicine (N,N-dihydroxyethyl glycine) Bicine is a very important buffer suitable for low-temperature biochemical environments. It can be used to prepare stable substrate solutions. It is one of the few buffers that can be used in special environments.     NO.3 HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) A non-toxic and harmless biological buffer, which can maintain a constant pH for a long time, and is loved by cell culturers.     NO.4 CAPS (3-(cyclohexylamine)-1-propanesulfonic acid) In the application of nucleic acid hybridization, CAPS can reduce the yield of non-specific hybridization products and increase the specificity of nucleic acid hybridization; at the same time, it can maintain the yield of target hybridization products; it is useful in the identification of specific pathogens, the diagnosis of genetic diseases, and the analysis of gene sequences. Important value. It is commonly used as a buffer in detection kits, such as biochemical diagnostic kits and DNA/RNA extraction kits. It can also be used in a variety of environmentally friendly high-quality water-based two-component polyurethane coatings, and can be used in water-based isohydrogen ester curing agents. It can be combined with active groups (hydroxyl, carboxyl, amino, epoxy, etc.) Resin coexists stably for a long time.     NO.5 MOPS (3-morpholinopropane sulfonic acid) MOPS does not form complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. It can be commonly used in the culture medium of bacteria, yeast and mammalian cells. It is also used as a running buffer in electrophoresis and a buffer in protein purification chromatography.

Do all vacuum blood collection tubes need additives?   Vacuum blood collection tubes are an important tool for virus detection in hospitals and play a pivotal role in the field of medical and health care. She is an important blood test equipment and apparatus. Additives for blood collection tubes are a key factor affecting the function of blood collection tubes. Commonly used additives are anticoagulants, coagulants, buffers, protective agents, separating gels, etc. This article will introduce several common blood collection tube anticoagulants and their effects. Anticoagulant, as the name implies, is a chemical agent that prevents blood from clotting. Commonly used anticoagulants for blood collection tubes are heparin sodium, heparin lithium, sodium citrate, potassium oxalate and ethylenediaminetetraacetic acid (EDTA).     Heparin is considered to be the best anticoagulant in the determination of blood chemical composition. Sodium and lithium salts are commonly used in clinical blood tests and have unique application value. Heparin is mainly found in blood collection tubes with green caps, and is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, blood rheology and emergency biochemical determination.     Lithium heparin has the least possibility of interference when detecting non-lithium ions, and has better anticoagulant activity than heparin sodium. At present, heparin lithium is gradually replacing heparin sodium in blood tests. Sodium citrate, citrate can form a soluble chelate with calcium ions in the blood, thereby preventing blood clotting. It is generally used for checking blood coagulation and blood cell deposition rate.     Sodium citrate is used as an anticoagulant in anticoagulant tubes with light blue caps and ESR tubes with black caps. Potassium oxalate is an anticoagulant with high dissolution concentration and strong anticoagulant effect. The principle of action is that oxalate and blood calcium ions form calcium oxalate precipitation and tissue coagulation. It is often used for anticoagulation of blood samples for testing. Potassium oxalate is often mixed with sodium fluoride, and it is present in the gray cap anticoagulation blood collection tube.     Ethylenediaminetetraacetic acid (EDTA) is one of the most commonly used and most important anticoagulants and reagents in clinical examinations. EDTA can combine with calcium ions in the blood to form a chelate, and Ca2+ loses the coagulation effect, thereby preventing blood coagulation. It is suitable for a number of hematological examinations. The commonly used EDTA anticoagulant is EDTA-2K. The EDTA purple tube cap anticoagulant blood collection tube is suitable for whole blood and plasma testing. It is the first choice for blood routine, glycosylated hemoglobin, and blood group testing.     In summary, anticoagulants play an important role in blood vessel detection, but not all blood collection tubes contain anticoagulants. In blood collection tubes of different colors, green, blue, black, gray and purple caps are used in blood collection tubes. Contains anticoagulant, EDTA-2K is mainly used in purple anticoagulant tube.     Desheng Biotech specializes in the production of blood collection tube additives and has 15 years of production and research and development experience. Committed to providing complete blood testing solutions for hospitals, clinics and scientific research institutions across the country. Contains a series of products such as EDTA, lithium heparin, and anti-irradiation gel.

What is the significance of the stability study of in vitro diagnostic reagents   In vitro diagnostic reagents are widely used in medical research and clinical testing, and are an important indicator of the quality of clinical diagnostic information. In-vitro diagnostic reagents generally refer to products and services that are outside the human body, and obtain relevant clinical diagnostic information by testing the body, including blood, body fluids, and tissue samples, which can help judge diseases or body functions. The stability of in vitro diagnostics is an indicator that guarantees its quality and accuracy of test results. It is an essential attribute of reagents and an important reference for the production, transportation, storage and use of in vitro diagnostic reagents.     In vitro diagnostic reagent stability tests usually include real-time and actual stability, accelerated stability, bottle opening or unsealing stability, reconstitution stability, sample stability, transportation and stability, and reagent and sample storage stability. The purpose of these stability studies is to determine the transportation, storage and storage conditions of reagent products after opening, and to determine the shelf life of the product and the shelf life after opening. In addition, it can also verify that the stability of the product changes when the storage conditions and shelf life are changed, so as to evaluate and adjust the product's composition, process, and packaging materials based on the results.     Take the index of actual and sample storage stability as an example. This index is one of the important factors that affect the effect of in vitro diagnostic reagents. The storage of reagents should be placed and stored in strict accordance with the instructions. For example, for freeze-dried powder reagents containing peptides, the water content and oxygen content in the storage environment have a great influence on the stability of the reagents. Therefore, unopened freeze-dried powders should be stored in a refrigerator in a refrigerator as much as possible.     Samples to be tested, such as samples processed by medical institutions after collection, should be stored as required according to their performance and risk factors. During routine blood tests, the blood and anticoagulant should be shaken and the samples should be placed at room temperature (about 20°C). ) After 30 minutes, 3h, and 6h, the machine will be tested. Some special samples, such as nasopharyngeal swab samples collected during the nucleic acid test of the new coronavirus, need to use a virus sampling tube containing a virus preservation solution, and the specimens used for virus isolation and nucleic acid testing should be tested as soon as possible, within 24 hours The tested specimens can be stored at 4°C; the specimens that cannot be tested within 24 hours should be stored at -70°C or below (if there is no storage condition of -70°C, they should be temporarily stored in the refrigerator at -20°C).     Hubei New Desheng Materials specializes in the production and development of in vitro diagnostic reagents, chemiluminescence reagents, luminescent substrates, biological buffers, blood collection tube additives and enzyme preparations. Desheng has been established for decades and has its own R&D team. It has been researching and developing in vitro diagnostic reagents for many years. It has many kinds of chemical reagent products under its umbrella. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Gold nanostars, there may be many people who don’t know what it is. Gold nanostars refer to gold particles that are shaped into stars. The gold particles can be sent to a tumor, and then manipulated by a laser or magnetic field, and ultimately destroy them by heating the malignant tumor cells. Nano Venus makes the burning hotter. Nano-Venus is a new material for attacking cancer. Now, researchers point out that gold particles can burn even hotter if they are shaped into stars.   Among them, the common method for synthesizing gold nanostars is the non-nuclear and surfactant-free strategy using Good’s buffers HEPES, EPPS and MOPS. These reagents can be used as metal reducing agents, shape directing agents and stabilizers. Nanostars of different sizes and structures can be obtained by adjusting the buffer concentration and the pH of the solution. And what is the connection between HEPES and gold nanostars? Although HEPES is conducive to the formation and structural stability of gold nanostars, we know very little about the interaction between HEPES and metals. Recently, the remodeling of gold nanostars induced by acidification has been studied, and it was found that its morphology depends on pH and acid composition, and is also affected by the protonation state of HEPES. The zeta potential and DFT method were used to measure the change of the molecular proton state. Surface enhanced Raman (SERS) revealed that the change of pH induces the reversible activation of the amine and sulfonic acid groups of HEPES, and the redistribution of electrons weakens the affinity of HEPES to metals, thereby facilitating adsorption.   Experimental results show that the reconstruction of gold nanostars depends on the protons in the solution, which is consistent with the decomposition behavior of nanospheres with a diameter of about 6 nanometers under acid induction. In addition, the strength and reconstruction rate of gold nanostars have a certain relationship with the affinity of anions and gold. Through research on the related effects of gold nanostars and HEPES, it is found that HEPES is partially substituted by benzene. When benzene adsorption or the chemical enhancement related to the sulfonic acid group of HEPES weakens, HEPES will reorient. The study speculated that the partial desorption of HEPES and the change of electron distribution caused the reorientation.   Hubei New Desheng Materials Co., Ltd. specializes in biochemical raw material manufacturers. It can provide various specifications of biological buffers HEPES, Tris, BICINE, CAPS, TAPS, etc., chemiluminescence reagents, blood collection tube additives, chromogen substrates, enzyme preparations, antigens Antibodies, carbomers, etc., please call for more details!

Bis-Tris, Tricine, TES, TAPS are all buffers frequently used in biochemistry experiments and molecular biology experiments, and their molecular structures all contain the molecular structure of Tris. So what are the differences between these buffers and Tris in use? What are the similarities or differences between them? Here is a summary of these buffers.   Tris Tris (Tris) has a pKa of 8.06 at 25°C and a buffer range of 7.0~9.0. The commonly used experiments are as follows: (1) Commonly used in gel electrophoresis buffer, such as TAE or TBE; (2) Commonly used to prepare Laemmli buffer; (3) Often prepare running buffer and loading buffer together with glycine and SDS; (4) It can be used as binding buffer and eluent in anion exchange chromatography; (5) It is used as a buffer for RNA hybridization and DNA lysis.   In the use of Tris, you need to pay attention: the pH value of Tris buffer is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process; the primary amino group of Tris reacts with various molecules to a certain extent, including RNA Enzyme inhibitors, aldehydes, common metals such as Cr3+, Fe3+, Ni2+, Co2+ and Cu2+, enzymes, and DNA. In addition, Tris is not suitable for bicinchoninic acid (BCA) determination.   Bis-Tris Bis-Tris, bis(2-hydroxyethyl)amino(trihydroxymethyl)methane, zwitterionic buffer, pKa of 6.46 at 25°C, pH buffer range of 5.8~7.2. Its application areas are as follows: (1) As sample buffer, gel buffer and running buffer for various types of electrophoresis; (2) As a buffer in anion exchange chromatography; (3) X-ray crystallographic studies used in the crystallization process of haloalkane dehalogenase; (4) With low conductivity and high sensitivity, it is an effective buffer for NMR spectroscopy; (5) Interact with human liver fatty acid binding protein (FAB) and affect protein dynamics.   Bis-Tris can form strong complexes with Cu2+ and Pb2+, and form weak complexes with many common metals. Therefore, when using in solutions containing metal ions, its complex constant should be considered. In addition, in systems with pressure changes, a Bis-Tris buffer solution can be used. Bis-Tris can replace highly toxic dimethylarsine buffer, but it is also not suitable for bicinchoninic acid (BCA) determination.   Desheng Biochemical is a high-tech enterprise focusing on the fields of life science and biotechnology. Based on the tenet of serving customers and serving scientific research, the company provides Chinese experts and scholars with a complete range of high-quality products. Tris, Tris-HCL, Bicine, CAPS, MOPS, TAPSHEPES, etc. have been widely used in life science basic research, development and application, pharmacy, disease diagnosis and control, population and health, biotechnology and many other fields. Customers are located in universities, research institutes, hospitals, health and epidemic prevention, commodity inspection and quarantine, pharmaceutical companies, biotechnology companies, and food industry units.

In order to resist the influence of a small amount of strong acid and based on biochemical research work, we often use an important reagent-buffer solution. Tris(hydroxymethyl)aminomethane, CAS corresponding number 77-86-1, is an important member of the buffer family, and is also widely used in the preparation of buffers in biochemistry and molecular biology experiments. It is also used for the preparation of surfactants and vulcanization accelerators; and the preparation of water-soluble polymers using Tris structural units as coating dispersants.   The advantages of TRIS buffers are also obvious. Because Tris base has a certain alkalinity, it can be used to prepare buffers with a wide range of pH from acidic to alkaline. Tris has little interference to the biochemical process and will not precipitate with calcium, non-heavy metal magnesium and heavy metal ions. There are two ways to prepare Tri-HCl buffer: use 0.05 mol/L Tris and 0.05 mol/L HCl solution, and then mix according to the volume listed in the common table. However, the standard concentration of dilute hydrochloric acid is not easy to obtain, so another method is usually used: Take 1 liter of 0.1 mol/L Tri-HCl buffer solution as an example: first, use 12.11 grams of Tris base to dissolve it in 950 mL~970 mL of deionized water, then add 4 N HCl while stirring. Use a pH meter to measure the pH of the solution to the desired pH, and then add 1L of water.   Tris buffer is a buffer widely used in biochemical research. The common effective pH range is in the "neutral" range, for example: Tris-HCl buffer: pH = 7.5~8.5 Tris-phosphate buffer: pH = 5.0~9.0 In the electrophoresis buffer solution, glycine can form a stable pH Value buffer system.   The Tris-HCl buffer system is used to stabilize the pH of the gel. It is also widely used as a solvent for nucleic acids and proteins. The synthesis of nematode intermediate fibers also uses the low ionic strength of Tris buffer. Add EDTA to Tris hydrochloric acid buffer to prepare TE buffer. This buffer can be used for DNA structure stabilization research and storage. The "TAE buffer" is obtained by substituting acetic acid for the pH adjusting acid solution, and the TBE buffer is obtained by substituting boric acid. Two commonly used buffers are used for nucleic acid electrophoresis experiments.   Desheng Biochemical is a high-tech enterprise specializing in biotechnology and life fields. The company serves customers and takes scientific research services as its purpose. Its high-quality products have been adopted by scholars and experts from all over the world. The production of TRIS, TRIS-HCl, BICINE/CAPS/MOPS, Taps/hepes and other products has been widely used in basic research, epidemic diagnosis, control, and basic research in the development or application of many fields such as biotechnology. Customers are all over universities, frozen commodity quarantine and inspection, pharmaceutical production companies, biotechnology companies, food industry and other departments.

Biological buffers are often used in biochemical research experiments and are of extremely important significance. They are a kind of conditioning fluid that can resist the influence of a small amount of strong acid and alkali from the outside and maintain the pH value basically unchanged. Among the commonly used biological buffers are Tris buffer solution, PBS buffer solution, CAPS buffer, MOPS buffer, TAPS buffer and EPPS buffer. This article will compare and introduce the most commonly used Tris buffer solution and PBS buffer solution from several aspects.   1. Buffer range Tris is a weak base with a molecular formula of C4H11NO3, a molecular weight of 121.14, and a pKa of 8.1 at 25 °C. The effective buffer range of its buffer is between pH 7.0 and 9.2. The pH values ​​commonly used in biochemical experiments are 6.8, 7.4, 8.0, and 8.8. Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments, and even has a tendency to exceed phosphate buffers.   Phosphate buffered saline (PBS) is a salt solution with phosphate as the pH buffer and sodium chloride as the osmotic pressure balance. Commonly used are sodium phosphate buffer and potassium phosphate buffer. Because of their secondary dissociation and a wide range of buffering pH values, they are the most widely used buffer solutions in biochemical research.   2. Configuration method There are two ways to prepare Tris-HCl buffer: prepare Tris and HCl solutions separately, and then mix according to the volumes listed in the common table. However, because standard concentration of dilute hydrochloric acid is not easy to prepare, another method is commonly used: Take the configuration of Tris-HCl buffer as an example: first dissolve Tris base in 950 mL～970 mL deionized water, add HCl dropwise while stirring, and use The pH meter measures the pH value of the solution to the required pH value, and then adds water to it.   The preparation method of phosphate buffer solution: Weigh NaCl, KCl, KH2PO4 and K2HPO4, dissolve them in 800 mL of distilled water, adjust the pH of the solution to 7.4 with HCl, and finally add distilled water to make the volume to 1 L. It can be stored in a refrigerator at 4 ℃. It should be noted that the usual concentration refers to the concentration of all phosphates in the buffer solution, not the concentration of Na+ or K+. Na+ and K+ are only used to adjust the osmotic pressure. Potassium salt is better than sodium salt when configuring phosphate buffer solution. Sodium salt is not easy to dissolve at low temperature, while potassium salt has relatively higher solubility.   3. Field of Use The Tris buffer solution forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; the Tris-HCl buffer system is used in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer is also It can be used to form the intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer" , Change to boric acid to get TBE buffer. These two buffers are often used in nucleic acid electrophoresis experiments.   Phosphate buffer solution Generally active biological preparations must be diluted with phosphate buffer solution. The reason is that it has a salt balance and a buffering effect that can adjust the appropriate pH value. Distilled water has no salt balance effect, which will destroy the structure and biological properties of biological proteins; physiological saline does not have the effect of adjusting pH, and it cannot ensure that it participates in biological reactions under optimal conditions for complete and active substances, so the use of PBS is Preferred. Of course, PBS is not a panacea. Some biologically active substances require relatively high conditions, and more ingredients need to be added to the balance buffer to maintain the best conditions to ensure that the biologically active substances maintain their most complete characteristics. Therefore, PBS is mainly used for cell experiments, and its buffer range is most suitable for neutral.   In the biochemical experiment, the buffer solution should be carefully selected, not only the buffer range of the buffer solution, but also the use environment of the buffer solution should be considered comprehensively. Hubei New Desheng Materials has been specializing in the production and development of biological buffers for many years. Desheng has an independent R&D team and has a wealth of practical experience in product development and production. At present, the biological buffers and other products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many foreign customers. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

At present, the domestic chemiluminescence immunoassay technology has been advancing by leaps and bounds, and is gradually in line with the level of international developed countries. Among them, chemiluminescence reagents are mainly developed around acridinium ester luminescence reagents and luminol reagents, so who will become the core C position of chemiluminescence reagents What?   Acridine ester chemiluminescence reagent The difference between luminol and acridine esters: 1. Acridine esters and luminol are both very widely used luminescent reagents in the chemiluminescence immunoassay CLIA. There is a big difference between the two. Compared with luminol, the most intuitive thing is the sensitivity. Much higher.   2. The price of acridine esters is much higher than that of luminol. The price of acridine esters luminescent reagents is usually priced in milligrams or grams, with an average of several hundred yuan per milligram; while luminol is priced in grams or kilograms, gram price It ranges from tens to hundreds, so the spread is still relatively large.   3. Luminol, isoluminol and their derivatives are the earliest types of chemiluminescent substances used, which require the use of catalyst peroxidase POD and enhancers, which will increase the background luminescence and increase the measurement background. This limits the sensitivity of this technology and its application and development.   4. Acridine ester has high luminous efficiency, simple luminous system, no need to add catalyst, low background, simple marking. Because the thermal stability of the traditional acridinium ester AE-NHS is not very good, after that, the acridinium ester derivative which is more stable than AE-NHS has been synthesized and applied to CLIA. For example, DMAE-NHS has been proven to have good thermal stability and luminescence properties.   The reaction sensitivity of acridine-based luminescent reagents is very high. The addition of the excitation solution causes the reaction system to immediately release photons of about 430nm. The protein concentration can be detected by counting the number of photons with a standard luminometer. Because this light-emitting process is very short (the whole process is completed within 2 seconds), the sample must be placed directly in front of the photon detector inside the photometer. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridinium esters. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons.   Acridine esters are obviously superior to luminol in all aspects of CLIA, but its price and equipment cost are higher. In some cases where the detection requirements are relatively low, it is not necessary to use acridine esters.

As a chemiluminescent reagent, there are many different models of acridinium ester. Among them, there is an acridinium ester NSP-DMAE-NHS, which has a labeling effect on nucleic acids. I believe many people may not know it. We will introduce this Details of the acridine esters.   Acridinium ester NSP-DMAE-NHS, CAS number 194357-64-7, appearance is yellow powder, it is a very important chemiluminescence reagent. It has the advantages of mild reaction conditions, good reproducibility, high luminous efficiency, and strong luminous intensity. It is widely used in the fields of inorganic and organic compounds, environmental monitoring, biological and pharmaceutical analysis, and it is also widely used in sensitive detection of various types of diseases. And diagnosis.   In terms of in vitro diagnosis, acridinium ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Therefore, a method of how to label nucleic acids with acridinium esters will be introduced below.   Nucleic acid is the most important substance in all biological molecules. It is widely present in all animal and plant cells and microorganisms. Nucleic acid is divided into two categories: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, germs or parasites in the environment. Therefore, analysis of virus specific sequence DNA Conducive to the control of the epidemic.   In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation.   This method for labeling nucleic acids with acridinium esters mainly includes three steps. Firstly, the 5'and 3'ends of the DNA probes are protected respectively; then the acridinium ester labeling is carried out, and finally the DNA is purified and separated by HPLC. Among them, acridinium ester labeling is the most important. Dissolve 25mM acridinium ester in dimethyl sulfoxide (DMSO), configure 1M HEPES buffer (PH=8.0), and follow the molar ratio of nucleic acid probe: acridinium ester=1:5 Add to HEPES buffer and react at 37°C for 1h.   This method creatively labeled acridinium esters on both ends of the DNA, further enhancing the sensitivity of detection. At Hubei New Desheng Materials Co., Ltd., we have many years of experience in the production and development of acridinium esters. A lot of energy has been invested in the research and development of acridine esters. At present, the company's products have been sold to more than 100 countries around the world, and most of them have received good reviews for repurchase. The product quality is excellent and the price is favorable. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

There are many types of blood collection tube additives, and after these additives are added to blood collection tubes, they are generally distinguished according to the color of the blood collection tube cap. Vacuum blood collection tubes are important blood testing equipment and instruments. The use of additives in blood collection tubes is a key factor affecting the function of blood collection tubes. Commonly used additives include anticoagulants, coagulants, buffers, protective agents, separating gels, etc. And what are the anticoagulant products, and how much do you know?   As the name suggests, anticoagulants are chemical reagents that prevent blood from clotting. Commonly used anticoagulants for blood collection tubes are sodium heparin, lithium heparin, sodium citrate, potassium oxalate and EDTA.   Heparin is considered to be the best anticoagulant in the determination of blood chemical composition. Sodium and lithium salts are commonly used in clinical blood tests and have unique application value. Heparin is mainly found in blood collection tubes with green caps, and is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, blood rheology and emergency biochemical determination.   Lithium heparin has the least possibility of interference when detecting non-lithium ions, and has better anticoagulant activity than heparin sodium. At present, heparin lithium is gradually replacing heparin sodium in blood tests.   Sodium citrate is also known as: sodium citrate. Citrate can form soluble chelate with calcium ions in the blood to prevent blood clotting. It is generally used for checking blood coagulation and blood cell deposition rate.   Sodium citrate is used as an anticoagulant in anticoagulant tubes with light blue caps and ESR tubes with black caps.   Potassium oxalate is an anticoagulant with high dissolution concentration and strong anticoagulant effect. The principle of action is that oxalate and blood calcium ions form calcium oxalate precipitation and tissue coagulation. It is often used for anticoagulation of blood samples for testing.   Potassium oxalate is often mixed with sodium fluoride, and it is present in the gray cap anticoagulation blood collection tube.   EDTA is one of the most commonly used and most important anticoagulants and reagents in clinical examinations. EDTA can combine with calcium ions in the blood to form a chelate, and Ca2+ loses the coagulation effect, thereby preventing blood coagulation. It is suitable for a number of hematological examinations. The commonly used EDTA anticoagulant is EDTA-2K.   The EDTA purple tube cap anticoagulant blood collection tube is suitable for whole blood and plasma testing. It is the first choice for blood routine, glycosylated hemoglobin, and blood group testing.   It has been learned from the above that anticoagulants play a vital role in blood collection tubes, but in addition to anticoagulants, there are many other types of blood collection tube additives. And our company is a manufacturer specializing in the production of blood collection tube additives. The products we produce blood collection tube additives include: heparin sodium, heparin lithium, serum separation gel, EDTA-2K (3K), EDTA-2NA, coagulant, and high-efficiency coagulation accelerator Powder, water-soluble siliconizing agent, oleosilicon, potassium oxalate, trisodium citrate, sodium fluoride, etc. If you want to know about the products, you can call for consultation. Hubei Xindesheng Material Technology Co., Ltd. welcomes your calls.