China Wuhan Desheng Biochemical Technology Co., Ltd Company News

At present, the domestic chemiluminescence immunoassay technology has been advancing by leaps and bounds, and is gradually in line with the level of international developed countries. Among them, chemiluminescence reagents are mainly developed around acridinium ester luminescence reagents and luminol reagents, so who will become the core C position of chemiluminescence reagents What?   Acridine ester chemiluminescence reagent The difference between luminol and acridine esters: 1. Acridine esters and luminol are both very widely used luminescent reagents in the chemiluminescence immunoassay CLIA. There is a big difference between the two. Compared with luminol, the most intuitive thing is the sensitivity. Much higher.   2. The price of acridine esters is much higher than that of luminol. The price of acridine esters luminescent reagents is usually priced in milligrams or grams, with an average of several hundred yuan per milligram; while luminol is priced in grams or kilograms, gram price It ranges from tens to hundreds, so the spread is still relatively large.   3. Luminol, isoluminol and their derivatives are the earliest types of chemiluminescent substances used, which require the use of catalyst peroxidase POD and enhancers, which will increase the background luminescence and increase the measurement background. This limits the sensitivity of this technology and its application and development.   4. Acridine ester has high luminous efficiency, simple luminous system, no need to add catalyst, low background, simple marking. Because the thermal stability of the traditional acridinium ester AE-NHS is not very good, after that, the acridinium ester derivative which is more stable than AE-NHS has been synthesized and applied to CLIA. For example, DMAE-NHS has been proven to have good thermal stability and luminescence properties.   The reaction sensitivity of acridine-based luminescent reagents is very high. The addition of the excitation solution causes the reaction system to immediately release photons of about 430nm. The protein concentration can be detected by counting the number of photons with a standard luminometer. Because this light-emitting process is very short (the whole process is completed within 2 seconds), the sample must be placed directly in front of the photon detector inside the photometer. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridinium esters. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons.   Acridine esters are obviously superior to luminol in all aspects of CLIA, but its price and equipment cost are higher. In some cases where the detection requirements are relatively low, it is not necessary to use acridine esters.

As a chemiluminescent reagent, there are many different models of acridinium ester. Among them, there is an acridinium ester NSP-DMAE-NHS, which has a labeling effect on nucleic acids. I believe many people may not know it. We will introduce this Details of the acridine esters.   Acridinium ester NSP-DMAE-NHS, CAS number 194357-64-7, appearance is yellow powder, it is a very important chemiluminescence reagent. It has the advantages of mild reaction conditions, good reproducibility, high luminous efficiency, and strong luminous intensity. It is widely used in the fields of inorganic and organic compounds, environmental monitoring, biological and pharmaceutical analysis, and it is also widely used in sensitive detection of various types of diseases. And diagnosis.   In terms of in vitro diagnosis, acridinium ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Therefore, a method of how to label nucleic acids with acridinium esters will be introduced below.   Nucleic acid is the most important substance in all biological molecules. It is widely present in all animal and plant cells and microorganisms. Nucleic acid is divided into two categories: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, germs or parasites in the environment. Therefore, analysis of virus specific sequence DNA Conducive to the control of the epidemic.   In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation.   This method for labeling nucleic acids with acridinium esters mainly includes three steps. Firstly, the 5'and 3'ends of the DNA probes are protected respectively; then the acridinium ester labeling is carried out, and finally the DNA is purified and separated by HPLC. Among them, acridinium ester labeling is the most important. Dissolve 25mM acridinium ester in dimethyl sulfoxide (DMSO), configure 1M HEPES buffer (PH=8.0), and follow the molar ratio of nucleic acid probe: acridinium ester=1:5 Add to HEPES buffer and react at 37°C for 1h.   This method creatively labeled acridinium esters on both ends of the DNA, further enhancing the sensitivity of detection. At Hubei New Desheng Materials Co., Ltd., we have many years of experience in the production and development of acridinium esters. A lot of energy has been invested in the research and development of acridine esters. At present, the company's products have been sold to more than 100 countries around the world, and most of them have received good reviews for repurchase. The product quality is excellent and the price is favorable. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

There are many types of blood collection tube additives, and after these additives are added to blood collection tubes, they are generally distinguished according to the color of the blood collection tube cap. Vacuum blood collection tubes are important blood testing equipment and instruments. The use of additives in blood collection tubes is a key factor affecting the function of blood collection tubes. Commonly used additives include anticoagulants, coagulants, buffers, protective agents, separating gels, etc. And what are the anticoagulant products, and how much do you know?   As the name suggests, anticoagulants are chemical reagents that prevent blood from clotting. Commonly used anticoagulants for blood collection tubes are sodium heparin, lithium heparin, sodium citrate, potassium oxalate and EDTA.   Heparin is considered to be the best anticoagulant in the determination of blood chemical composition. Sodium and lithium salts are commonly used in clinical blood tests and have unique application value. Heparin is mainly found in blood collection tubes with green caps, and is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, blood rheology and emergency biochemical determination.   Lithium heparin has the least possibility of interference when detecting non-lithium ions, and has better anticoagulant activity than heparin sodium. At present, heparin lithium is gradually replacing heparin sodium in blood tests.   Sodium citrate is also known as: sodium citrate. Citrate can form soluble chelate with calcium ions in the blood to prevent blood clotting. It is generally used for checking blood coagulation and blood cell deposition rate.   Sodium citrate is used as an anticoagulant in anticoagulant tubes with light blue caps and ESR tubes with black caps.   Potassium oxalate is an anticoagulant with high dissolution concentration and strong anticoagulant effect. The principle of action is that oxalate and blood calcium ions form calcium oxalate precipitation and tissue coagulation. It is often used for anticoagulation of blood samples for testing.   Potassium oxalate is often mixed with sodium fluoride, and it is present in the gray cap anticoagulation blood collection tube.   EDTA is one of the most commonly used and most important anticoagulants and reagents in clinical examinations. EDTA can combine with calcium ions in the blood to form a chelate, and Ca2+ loses the coagulation effect, thereby preventing blood coagulation. It is suitable for a number of hematological examinations. The commonly used EDTA anticoagulant is EDTA-2K.   The EDTA purple tube cap anticoagulant blood collection tube is suitable for whole blood and plasma testing. It is the first choice for blood routine, glycosylated hemoglobin, and blood group testing.   It has been learned from the above that anticoagulants play a vital role in blood collection tubes, but in addition to anticoagulants, there are many other types of blood collection tube additives. And our company is a manufacturer specializing in the production of blood collection tube additives. The products we produce blood collection tube additives include: heparin sodium, heparin lithium, serum separation gel, EDTA-2K (3K), EDTA-2NA, coagulant, and high-efficiency coagulation accelerator Powder, water-soluble siliconizing agent, oleosilicon, potassium oxalate, trisodium citrate, sodium fluoride, etc. If you want to know about the products, you can call for consultation. Hubei Xindesheng Material Technology Co., Ltd. welcomes your calls.

The global population base continues to grow, and the incidence of chronic diseases and tumors continues to increase. For the diagnosis of diseases, there are usually online testing and in vitro testing. In vitro diagnosis is widely used due to the accuracy of detection and the better experience of patients. Commonly used additives for blood collection tubes: serum separating gel, heparin sodium, lithium heparin, dipotassium EDTA, tripotassium EDTA, coagulant, siliconizer, sodium citrate, potassium oxalate. When it comes to the classification of in vitro diagnostics, there are also different standards for the classification of in vitro diagnostics. In vitro diagnosis is based on different test principles or test methods.   According to the technical level, the entire IVD market can be roughly divided into three technical levels: high, medium, and low. The low-end market is for manual or semi-automatic common enzyme-linked immunoassay products. The mid-range market can be divided into low-end (biochemical, blood testing, urine testing, etc.) and mid-to-high-end (chemiluminescence immune products, fluorescent quantitative PCR molecules). Diagnosis, etc.), and the high-end market mainly includes flow cytometry, high-quantity genetic equipment, and so on.   In terms of development trends, the industry has a trend of continuous platform upgrades, and each technology platform upgrade is brought about by changes in market segment growth/share. For example, in recent years, the improvement of the immunology technology platform has brought about the replacement of ordinary enzyme immunity products by chemiluminescence immune products is a good example.   According to the way of matching reagents, the in vitro diagnostic equipment is an open system and a closed system. The open system means that the diagnostic instrument can be used with multiple reagents, while the closed system means that the instruments and reagents of the same partner must be used together.   From this perspective, chemiluminescence corresponds to gene chips and gene sequencing, which are closed, and other general types are open. According to the different testing environment and conditions, in vitro diagnosis includes laboratory diagnosis and bedside diagnosis. POCT refers to a way to use portable analytical instruments or supporting reagents to quickly detect results at the sampling site. It has been rapidly developed and applied with its advantages of "portability, ease of operation, and timeliness of results".   Desheng Bio is mainly engaged in the research and development, sales and production of various biological diagnostic reagent-related products and raw and auxiliary materials. Its quality system has passed the German TUV demonstration and obtained CE and ISO13485 certificates. The company’s main products blood collection tube additives: serum separation gel, heparin sodium, lithium heparin, dipotassium EDTA, tripotassium EDTA, coagulant, siliconizer, sodium citrate, potassium oxalate reagents are widely used in medical diagnosis, quarantine, disease prevention In the fields of control and public health. The company has established long-term business contacts with companies in the European and African biodiagnostics industry, and has established long-term cooperative relationships with some domestic scientific research and academic research institutions.

With the development of science and technology and the emergence of various advanced instruments, clinical biochemical projects have put forward higher requirements for blood collection and separation. It not only requires rapid results, but also requires us to ensure the accuracy of the results of each project. . In the past, hospitals used ordinary drying tubes to separate blood samples. Recently, due to the development of related technologies, many hospitals have begun to use lithium heparin anticoagulation tubes and separation gel accelerator tubes to separate blood samples, and analyze their plasma (serum) after centrifugation. What are the advantages of these two types of vacuum blood collection tubes?   Blood is collected in the morning and tested in the laboratory. Generally, it should be placed at room temperature for 2-3 hours. During this period, the metabolic activity of the cells does not stop. As the storage time increases, the intracellular and extracellular substances transfer. Serum separation gel is a viscous fluid with a specific gravity of 1.05, which is between serum (1.02) and blood clot (1.08). Its structure contains a large number of hydrogen bonds. The association of hydrogen bonds forms a network structure. The viscous liquid of the network structure is thixotropic. When the separating gel and the coagulated blood are centrifuged in the same test tube, under the action of centrifugal force, the network structure is destroyed and becomes a low-viscosity fluid, causing a rotation phenomenon. The blood clot heavy by the separating gel is transferred to the bottom of the test tube, and the separating gel forms a gel-like isolation layer between the serum and the blood clot, blocking and reducing the passage of blood cells to affect the serum components, thereby stabilizing the substances in the serum.   The anticoagulation of the lithium heparin tube can maintain the integrity of cells, delay the precipitation of the myocardial enzyme spectrum in the red blood cells, and the separation rubber tube can quickly accelerate the coagulation to release a part of the myocardial enzyme spectrum from the blood cells. After centrifugation, the separation of blood cells and serum has a certain relationship.   Therefore, the determination of myocardial enzyme spectroscopy is greatly affected by the precipitation of substances in red blood cells. The separation hose and the lithium heparin tube can precipitate serum or plasma quickly, reducing errors caused by sample placement, less hemolysis, less impact on the results, and more realistic results, etc. advantage. Relatively speaking, the separation hose separates blood cells and serum so that the result is closer to the situation at the time of blood collection. Therefore, when conditions permit, we should use separation hoses or lithium heparin tubes to dispense myocardial zymogram specimens as much as possible. If ordinary drying tubes are used to dispense myocardial zymogram specimens, serum should be separated as soon as possible after blood sampling.

At present, the new crown virus has swept the world and has caused serious impacts on human daily life. Nucleic acid detection is a detection method for the new coronavirus. In the specific process of nucleic acid detection, nucleic acid extraction is a very critical link. According to public reports, reagents such as TRIS and EDTA play an important role in nucleic acid extraction.   Nucleic acid is a biological macromolecular compound formed by the polymerization of many nucleotides, including deoxyribonucleic acid and ribonucleic acid. It is one of the most basic substances of life. Nucleic acid extraction refers to the process of separating nucleic acids from samples using physical and chemical methods. It is the prerequisite technology for a series of molecular biological analysis such as nucleic acid amplification, DNA fragment connection, vector construction, and high-throughput sequencing. It is a life science It is a very important technology in research, biological timely application and genetic diagnosis. Therefore, the extraction of nucleic acid is of great significance.   Generally speaking, nucleic acid extraction is a multi-step work. First, biological sample materials such as cells and tissue materials need to be crushed to inactivate nuclease and release nucleic acid. The role of TRIS, EDTA and other reagents is mainly reflected in the release of nucleic acid. .   Nucleic acids are easily hydrolyzed in acidic solutions and are more stable in neutral or weakly alkaline solutions. And TRIS is Tris, pH buffer range: 7.0-9.0, can maintain the stability of the nucleic acid released after the lysis of the sample to be extracted, to avoid the degradation of the nucleic acid, and improve the concentration and purity of the nucleic acid.   EDTA is ethylenediaminetetraacetic acid, which can combine with metal ions such as Mg2+, Ca2+, Mn2+, Fe2+, and can prevent metal ions from activating proteases, thereby reducing the impact of metal ions on nucleic acid quality.   In summary, TRIS, EDTA and other reagents can fully and effectively lyse cells during the nucleic acid extraction process, so that the nucleic acid in the cell can be fully released, and a higher concentration of nucleic acid can be obtained, which is beneficial to improve the quality of nucleic acid and ensure the accuracy of subsequent operations. .   Hubei New Desheng Materials has been established for decades, specializing in the production and development of biological buffers, enzyme preparations, blood collection tube additives, chemiluminescence reagents, carbomers and other products. At present, Desheng's products have been sold to many countries around the world. The product quality is reliable and can meet the needs of customers in different industries. The price is low and can be discussed in detail, and the price can be controlled according to the required quantity. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Desheng Technology is a new high-tech enterprise of new materials. It is located in the famous "City of Hundred Lakes" and "Hometown of Fish and Rice" Hubei-Ezhou. The company has a group of high-quality production personnel, a strong technical team with strong development capabilities, and Coping with and solving various complex technical problems, has always been known for its good business reputation. It mainly produces several major types of products such as biological buffers, chemiluminescence reagents, display reagents, and blood collection tube additives. Among them, Tris (TRIS) is one of Desheng's core products.   In fact, there are many Tris (TRIS) on the market. As long as you search on the search engine, you can find a lot of business information about TRIS. But why does Desheng still make their main products in this seemingly saturated product? This has to start with their advantages in all aspects.   1. Desheng is a direct manufacturer of Tris The production unit of Tris is mainly based on factories. Although some laboratories also synthesize some, but the output is very limited and can only be self-sufficient and cannot meet the market demand. If the demand is relatively large or a large number of exports, look for production The manufacturer is relatively good. As a professional tris manufacturer, Desheng has high daily output and guaranteed quality. In addition to the price and cost advantage, it also has sufficient inventory as support.   2. Desheng has a strong R&D and production team Desheng Technology was founded in 2015 and is located in the famous "city of hundreds of lakes" and "land of fish and rice"-Ezhou City. The company has a group of high-quality production personnel, a strong technical team with strong development capabilities, and can deal with and solve various problems. Complex technical issues have always been known for their good business reputation.   3. Tris price concessions provided by Desheng You must have repeatedly compared the prices when you are looking for a Tris supplier. Here are some advices. It’s not good to only look for cheap. When choosing a merchant, we try to consider buying cost-effective products as much as possible. Although the TRIS produced by Shengsheng is not low-priced, it must be cost-effective. The price-performance ratio emphasizes performance and quality. Desheng can provide trial equipment for free, and you will know the cost-effectiveness.   4. Desheng provides good after-sales service Regardless of the purchase of any product, after-sales service must be taken into account, so you don’t have to worry about product quality problems. All Desheng products can be returned and exchanged if they encounter quality problems. This is something non-manufacturers cannot promise. As for There is no need to worry about the delivery date and the like. Desheng is also very advantageous in terms of long-term service and maintenance.   Under various advantages, our TRIS products have always been the first choice of customers. Good products, preferential prices, good after-sales and the provision of samples will all be the basis for us to take TRIS as our main product. Don't be attracted by all kinds of advertisements, but believe that the eyes of the people are discerning. The products that everyone is choosing are definitely worth buying. Please remember that the Hubei Tris (TRIS) supplier is here.

The chemiluminescence assay is becoming more and more popular due to its superb detection sensitivity. Commonly used are luminol, isoluminol, and acridine esters. In chemical analysis, chemiluminescence reactions are used to detect analytes directly covalently labeled with chemiluminescence compounds. Triggering a chemiluminescent label for a luminescent reaction generates a signal for the detection of the analyte. The advantage of this method is that it is simpler and clearer, and avoids the need for larger, relatively "sticky" labels. However, the factors for selecting the detection reaction, whether it is an enzyme label or a direct label, and how to determine the light intensity must be considered before designing the reaction. Enzyme labeling or direct labeling? The common method of generating chemiluminescence is to use labeled enzymes to catalyze the chemiluminescence reaction. Each label can initiate multiple chemiluminescence reactions, so usually only one or a few enzyme labels/analytes need to be incorporated. The chemiluminescent compound is supplied in excess as an enzyme substrate to ensure saturation kinetics. The light intensity is a linear function of the amount of labeled enzyme. Intensity, the emission rate of photons per second, is the product of the catalytic conversion of the substrate and the lifetime of the luminescent compound.   The chemiluminescence intensity/time distribution graph includes an initial rising period and extending the luminescence until the plateau or false plateau. If there is no stable plateau value, it indicates that the substrate is exhausted or the enzyme is inactivated. The detection of chemiluminescence produced by enzymes provides great flexibility in the measurement process. The light intensity at any point in time passing through the high point may be related to the amount of enzyme. If the speed is a single-point or multi-point slope type measurement problem, you can measure it in the ascending part. In order to obtain the highest sensitivity, however, there are not many suitable chemiluminescent compounds. Appropriate candidates must first have certain functions to allow connection with other molecules. More importantly, once the tag is triggered, it should emit all the light in the shortest possible time.   When chemiluminescence is gradually emitted over a period of time, the signal intensity (photons/second) decreases, and it is best to determine the optimal number of chemiluminescence labels on the species to be detected based on experience. Even if theoretically more tags should provide more photons, if the tags are spaced too close together, a quenching effect will occur. The surface area and the number of derivatizable groups (usually amino or mercapto groups) provide further restrictions. In practice, up to 10-20 labels can be bound to one macromolecule. In addition, the more labels on the surface of the binding molecule, the more likely the label will interfere with its binding properties.   Desheng Bio is a high-tech enterprise specializing in the production and research and development of chemiluminescence reagents. It provides stable supply of luminol, isoluminol, and acridine esters, including DMAE-NHS/Me DMAE-NHS/NSP-DMAE- NHS /NSP-SA/ NSP-SA-NHS /NSP-SA-ADH/, with exclusive core patents, has been unanimously approved by domestic and international friends.

The principle and classification of chemiluminescence reagents, there are three types of luminescence in nature: physical luminescence, chemiluminescence and bioluminescence. The narrow sense of luminescence immunoassay mainly refers to chemiluminescence. Chemiluminescence is an oxidation chemical reaction. After the electron is excited, it returns to the ground state and releases energy in the form of photons. The number of released photons is used to reflect the intensity of the chemical reaction. The commonly used luminescent agent is aminophthalic hydrazide (isoluminol and luminol). Connaught), acridine esters. Chemiluminescence immunoassay is a non-radioactive immunoassay method that has developed rapidly in the past 30 years, and it is a kind of ultra-high-sensitivity micro-assay technology. It combines with the immune response through the chemiluminescence system, and uses chemiluminescence-related substances to label antibodies or antigens. After reacting with the antigen or antibody to be tested, the free chemiluminescence markers are separated, and other related substances of the chemiluminescence system are added. Chemiluminescence, quantitative or qualitative detection of antigen or antibody.   Advantages of chemiluminescence immunoassay: (1) High sensitivity: theoretically the highest sensitivity of chemiluminescence can reach 10-18Mol/L. (2) Short detection time: The time for measuring the optical signal of each sample is no more than a few seconds, and it can be completed within 9-60 minutes from sample addition to analysis result. (3) Wide detection range: Theoretically, the linear range can be up to 105 relative luminous units (RLU), which is 5 orders of magnitude. (4) The analytical method is simple and fast: most analytical determinations are in a one-step mode in which only one reagent (or a composite reagent) is added. (5) High sensitivity: more than RIA. (6) Good safety and long service life: exempt the use of radioactive materials. So far, its hazards have not been discovered; the reagents are stable, and the shelf life can be up to six months to more than one year.   Disadvantages of chemiluminescence immunoassay: (1) The light-emitting process is short (2) High background (3) High instrument failure rate (4) Poor reagent stability (5) The detection accuracy is not high   Application of chemiluminescence immunoassay: Chemiluminescence immunoassay has unique characteristics of high sensitivity, high specificity, speed, accuracy, specificity, and automation. It plays an important role in clinical testing, drug analysis, environmental monitoring and other fields. Especially the determination of various hormones, tumor markers, drug concentration and other trace biologically active substances.   The clinical application of chemiluminescence immunoassay is mainly reflected in the treatment of thyroid hormones, adrenal/pituitary hormones, anemia factors, tumor markers, infectious diseases, allergic diseases, therapeutic drug monitoring, etc., to ensure the stability of the data results reliable. Among them, the detection of tumor markers has certain application value in the early detection of tumors, the development of the disease, the evaluation after treatment, and the monitoring of recurrence and metastasis.   Desheng Biotechnology has been doing chemiluminescence reagents for many years. In addition to chemiluminescence reagents, there are also other series of products such as blood collection tube additives, biological buffers, and chromogen substrates. Chemiluminescence reagents have been developed and produced by Desheng Biotechnology for many years. The products can fully meet the requirements of various manufacturers, with reliable quality and preferential prices. If you are interested, you can call to discuss in detail.

Chemiluminescence is molecular electromagnetic radiation caused by light produced by a chemical reaction. Because of its high sensitivity, good specificity, wide application range, simple equipment and wide linear range, chemiluminescence reactions have received extensive attention in various fields of life sciences, detective detection, food safety, and drug analysis. A good chemiluminescence reaction is determined by the intensity and sensitivity of the reaction. The following is a brief analysis of the influencing factors. Chemiluminescence labeling chemistry and labeling quantitative chemistry require high specific activity, and therefore require high detection sensitivity of the label material itself. The detection limit of conventional enzyme labels or fluorescent derivatives is about 1014 mol, which has been successfully applied to low-sensitivity measurement systems. The lower detection limit of luminol and acridine ester salts is about 10-18 mol of the available luminometer, so it matches or even exceeds the sensitivity of 1251.   Luminol and protein will greatly affect the quantum yield of the reaction. In particular, aminobutylethyl isoluminol can be combined with low molecular weight compounds without reducing the quantum yield. The compulsory method of first dissociation of the complex is to use the luminescence method. Quantum yield is an important indicator of the observation results when isoluminol is used to label hepatitis B antibodies.   When designing a detection scheme, there are many known chemiluminescence reactions to choose from. However, only a small number of known reactions have entered large-scale clinical diagnostic testing methods. There are several factors that influence the suitability of a particular chemiluminescence technique for automated immunoassays. First, the reaction should show sufficient chemiluminescence quantum yield.   All aspects of detection chemistry, including labeling methods, triggers, chemiluminescent reagents, are durable and reproducible. The method using enzyme labeling also faces another requirement, that is, the enzyme must be quite stable and easy to conjugate without reducing its catalytic activity. The ideal chemiluminescence reagent has an easy-to-measure signal through effective chemiluminescence reaction and predictable time course of luminescence.   Desheng Bio is committed to providing a variety of high-quality chemiluminescence reagents for the life science industry, including luminol, isoluminol, DMAE-NHS/Me DMAE-NHS/NSP-DMAE-NHS /NSP-SA/ NSP- SA-NHS /NSP-SA-ADH/. In order to further promote the development of innovative biomarkers in different fields to meet your basic, application and clinical research needs.

In recent years, chemiluminescence immunoassay methods have been increasingly favored by people due to their high sensitivity, strong specificity, wide application range, simple equipment required, and wide linear range. They are used in life sciences, clinical medicine, environment, and food. , Medicine and other fields have been widely used. Chemiluminescence immunoassay is an analysis technique that combines highly sensitive chemiluminescence detection with highly specific immune response to detect various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs.   What is chemiluminescence immunoassay? Chemiluminescence analysis is an analysis method that determines the content of a substance based on the intensity of the radiant light produced by a chemical reaction. Chemiluminescence immunoassay is to combine the chemiluminescence system with the immune response, label antibodies or antigens with chemiluminescence-related substances, and after reacting with the antigen or antibody to be tested, the free chemiluminescence markers are separated and added to the chemiluminescence system. Other related substances produce chemiluminescence for quantitative or qualitative detection of antigens or antibodies. The four most commonly used markers in chemiluminescence immunoassays are luminol, isoluminol and their derivatives, acridinium ester derivatives, peroxidase and alkaline phosphatase.   Classification of chemiluminescence immunoassay: According to the different labels and luminescence principles used in chemiluminescence, it can generally be divided into three categories: direct chemiluminescence immunoassay, enzymatic chemiluminescence immunoassay and electrochemiluminescence immunoassay. Different from the former two, electrochemiluminescence generates light signal by electrochemical reaction of electrochemiluminescence agent on the surface of the electrically activated electrode. Co-reactants are often needed to improve the luminous efficiency, such as tripropylamine (TPA) as ruthenium terpyridine ([ Rb(bpy)3]2+) electron donor co-reactant. Commonly used light-emitting systems are: acridine esters, luminol (luminol), terpyridine ruthenium, peroxy oxalate (TCPO, DNPO) and strong oxidant potassium permanganate, Ce (SO4) 2 etc. (see Chart 1 below). Some luminescent agents react slowly with oxidants, and they also need the role of catalysts and enhancers to improve luminous intensity and stability.   Application of chemiluminescence immunoassay: The chemiluminescence immunoassay method not only has the high sensitivity of chemiluminescence analysis, but also has the high specificity of immune response. It has a wide range of applications and development prospects in the fields of clinical diagnosis, food safety and environmental monitoring.   1. Fast chemiluminescence immunoassay method Traditional immunoassay methods often take several hours to complete. The long incubation time also leads to the adsorption of immunoreagents on the inner wall of the pipeline, which increases the signal crossover between different assays, thus limiting the detection throughput of samples and the effectiveness of immunoassay methods. Value. In order to overcome these shortcomings and achieve high throughput of immunoassays, the external field drive or effective solution mixing strategy can be used to accelerate the mass transfer rate of antigens, antibodies and other biological macromolecules, or increase the temperature of the reaction system to accelerate the kinetics of the immune response. , So as to achieve rapid immune detection.   2. Multi-component chemiluminescence immunoassay method The simultaneous realization of multi-component detection in a single analysis process has outstanding advantages such as high analysis throughput, short time required, less sample consumption, and low analysis cost. It is the long-term goal pursued by immunoassay and is also the research of immunoassay in recent years Hot spot.   3. Highly sensitive chemiluminescence immunoassay method In actual detection, the sensitivity and specificity of the existing detection methods are not ideal, and high-sensitivity and high-selectivity detection methods are increasingly showing their importance. The development of nanotechnology provides an opportunity for the development of highly sensitive chemiluminescence immunoassay methods. Nanoparticles have been widely used in biomarker analysis, and there have been a variety of signal amplification methods used in the construction of highly sensitive immunoassay methods.

Luminol, also known as luminol. The chemical name is 3-amino-phthalic hydrazide. It is a pale yellow powder at room temperature, which is a relatively stable synthetic organic compound. Luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract. Luminol is a chemiluminescent reagent, which can be converted into excited amino phthalic acid in the presence of hydrogen peroxide molecules, and then emits strong fluorescence.   Under normal circumstances, the chemiluminescence reaction of luminol and hydrogen peroxide is quite slow, but when there is a catalyst, the reaction is very rapid. The most commonly used catalysts are metal ions. Within a wide range of concentrations, the concentration of metal ions is proportional to the luminescence intensity, which enables chemiluminescence analysis of certain metal ions. This reaction can be used to analyze organic compounds containing metal ions. Achieve high sensitivity.     The second is to use certain compounds to determine the enhancement and inhibition of luminol on chemiluminescence. The initial application of luminol reagent was to detect proteins. In western blotting, an enzyme-linked antibody is often used to bind the protein to be detected, and the combination of luminol chemiluminescence reagent and enzyme (peroxidase) can make the local luminescence and reduce the protein The position is displayed. The luminescence in the picture is very bright, probably because of the chemiluminescence enhancer added to the reagent. Of course, the luminescence is far less bright when actually detecting proteins.   In addition, luminol derivatives such as isoluminol (ABEI) are labeled on carboxylic acid and ammonia compounds, and then separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then under alkaline conditions It reacts with hydrogen peroxide-potassium ferricyanide to perform chemiluminescence detection, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection. Desheng's current chemiluminescence reagents include acridinium ester, luminol, etc., and the quality is guaranteed and the supply is sufficient.