China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The full name of Tris-Hcl in Chinese is Tris Hydrochloride, another name is Tris Hydrochloride, and the English name is TRIS hydrochloride. I believe that many people are familiar with Tris. Tris has a wide range of applications. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses. Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fibers of the nematode nuclear laminin. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tris is not suitable. Try to replace it with a buffer that can be tolerated by other mass spectrometers.   Tris-HCl is derived from the reaction of Tris as the main raw material. Its solution is acidic and is mainly used as a biological buffer to adjust the pH of the virus preservation solution. If you need to adjust to neutral or alkaline, you can add Tris or NaOH to achieve. In addition to this information, what are the configuration methods for Tris-Hcl? How much do you know? The configuration method of Tris-Hcl will be introduced below.   Preparation method of Tris-HCl buffer: 1. Tris-Hcl (0.05mol/L, 25℃) After mixing 50ml 0.1mol/L tris (Tris) solution with x ml 0.1mol/L hydrochloric acid, add water to dilute to 100ml PH    x/ml     PH     x/ml 7.1    45.7     8.1    26.2 7.2    44.7     8.2    22.9 7.3    43.4     8.3    19.9 7.4    42.0     8.4    17.2 7.5    40.3     8.5     14.7 7.6    38.5     8.6      12.4 7.7    36.6     8.7     10.3 7.8     34.5    8.8     8.5 7.9     32.0    8.9     7.0   Tris molecular weight=121.14: 0.1mol/L solution is 12.114g/L. Tris solution can absorb carbon dioxide from the air, so the bottle cap should be tightly closed when using. When configuring Tris-Hcl solutions with different concentrations and different pH values, you can first prepare a 0.05mol/L solution with a certain pH value, and then dilute to the required concentration.   2. 1M Tris-Hcl (PH7.4, PH7.6, PH8.0) Component concentration 1M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 121.1g Tris and place it in a 1 L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Add the required PH value for the adjustment of the concentrated HCL amount according to the following value; PH value              Concentrated HCL 7.4                        Approximately 70ml 7.6                        about 60ml 8.0                        about 42ml 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving. 3. 1.5M Tris-Hcl (PH8.8) Component concentration 1.5M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 181.7g Tris in a 1L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Adjust the PH value to 8.8 with concentrated Hcl; 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving.   It should be noted that the solution should be cooled to room temperature before adjusting the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.   The above is the configuration method of Tris-Hcl provided by Hubei Xindesheng Material Co., Ltd. In addition to the production of Tris-Hcl, Desheng also produces other chemicals, such as blood collection tube additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer products. At present, Desheng company has been established for decades, has its own R&D team, and has invested a lot of energy in biological buffers. The quality of the products is reliable, the price is favorable, and if you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Since the COVID-19 pandemic, COVID-19 reagents have been on fire, and a little brother, the COVID-19 preservation solution, has been brought along with him. "Suddenly like a spring breeze coming all night", the preservation liquid also springed up like bamboo shoots after a rain. However, "squandering flowers are gradually becoming charming." Faced with hundreds of products, there is really no way to choose. Which points should I look at? 1. Are the raw materials of the virus transport medium qualified? Highly responsible virus preservation liquid manufacturers can not only fulfill their promises, but also ensure that the facts are described without exaggeration, such as the strict selection of raw materials to prevent fraud. The irresponsible manufacturers will only exaggerate in order to promote the order. In this process, customers should keep their eyes open and distinguish right from wrong.   2. Is it easy to grow bacteria? After the preservation solution is stored for a period of time, it is easy to spoil. The clear preservation solution becomes muddy, moldy, and bacteria grow, and it is embarrassing to be unsafe at night. The bacteria that grow out are not bad, I know they can't be used. Those who have been contaminated but will not grow long, hide in which box, which patient they are used on, and what impact they will have on the results, is known to all. Preventing pollution is undoubtedly a big challenge in the production and quality control process. Of course, it is not difficult to deal with, and measures such as cleaning the workshop and adding antibiotics can all be dealt with calmly.   3. Is it easy to leak? Because the virus preservation liquid is liquid, some preservation liquids are prone to leakage when they encounter rough handling or the negative pressure of air transportation during transportation, so the entire box and the whole box have to be scrapped. If the packaging is not in place and leaks, it will be very troublesome. Therefore, the selection of packaging materials, the design of the structure, and the manufacturing process must be checked.   4. Is the price reasonable? The price of any product is a very critical factor. Because of the epidemic, many companies’ conventional products are not selling well. Therefore, many companies in the market will sell shoddy products at low prices in order to survive. The price is low, but the quality is not guaranteed. The price and value are often corresponding. If the price of the virus transport medium is much lower than the market average price, then you must seriously consider whether its quality is reliable. Therefore, when choosing a virus transport medium manufacturer, be careful not to blindly pursue low prices.

Since the beginning of the new coronavirus pandemic, the unprecedented global demand for virus diagnostic tests (PCR tests) has led to a severe shortage in the supply chain. One of the most important links is the virus preservation medium (VTM) used to preserve and transport samples. liquid. Due to the shortage of virus transport mediums, many countries are forced to use alternative media for virus transport mediums, such as sterile saline, liquid Amis and other inactivated preservation media. However, the gap between these alternative media and the virus transport medium is also obvious, and they are not as good as the virus transport medium in terms of functionality, stability and inhibition.   The diagnostic test of the new coronavirus is to detect the viral nucleic acid by amplifying the specific sequence of SARS-CoV-2. SARS-CoV-2 is composed of extremely unstable and easily degraded RNA. Storing virus samples in qualified environmental conditions will lead to false negative results, and the presence of microorganisms will also contaminate virus samples. For these reasons, the Centers for Disease Control and Prevention in various countries regard the correct collection and storage of the new coronavirus COVID-19 as the most important step in diagnosis.   The preservation solution for other viruses has been optimized for decades. It was originally designed to preserve the viability of the culture, and later used for nucleic acid-based tests. It is a buffer solution, virus lysate, virus nucleic acid preservation agent, and antibacterial agent. The harmful mixture can protect the virus while eliminating the contaminating microbial flora that may interfere with the test. The general ingredients are: EDTA chelating agent, guanidine salt, anionic surfactant and cationic surfactant.   Virus transport medium is divided into two types: inactivated preservation solution and non-inactivation preservation solution. The inactivation preservation solution can inactivate the collected virus, while ensuring the integrity of the viral nucleic acid in the sample, and the collected virus sample can be Transport and long-term storage under normal temperature conditions; the non-inactivated storage solution can keep the virus active and will not inhibit subsequent nucleic acid amplification, but it needs to be stored in cold storage and tested as soon as possible.   Our company can provide inactivated and non-inactivated virus preservation liquid products with excellent quality and reasonable price. For more information, please contact us: Hubei Xindesheng Material Technology Co., Ltd. hosmakesmehappy@outlook.com

Acridine ester, a yellow powder, CAS No.: 194357-64-7, has high quantum yield and high chemiluminescence efficiency, which is five times or more than that of luminol. The chemiluminescence process of acridine ester is rapid, with low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. It is one of the most widely used chemiluminescence markers and plays an important role in chemiluminescence immunoassay.   Acridine ester Chemiluminescence immunoassay (CLIA) is a combination of highly sensitive chemiluminescence assay and highly specific immune reaction, which is used for the detection and analysis of various antigens, hapten, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. There are many kinds of chemiluminescence systems used in clinical examination, the most important are luminol and its derivatives, acridine ester, spiroamantane-1,2-dioxane and its derivatives, and electrochemiluminescence system.     Among various chemiluminescence systems, acridine esters are considered to be ideal substrates. What are the unique advantages of acridine esters compared with luminol system?   1. The luminescence system of acridine ester is simple and no catalyst is needed. Under alkaline conditions, acridine ester molecules can be attacked by hydrogen peroxide to produce dioxane, which is unstable and decomposes into CO2 and n-methylacridone in electronic excited state. When it returns to the ground state, it emits light with a wavelength of 430nm without catalyst, and the luminescence system is simple.   2. Acridine ester has high luminous efficiency and intensity. Acridine ester chemiluminescence is a flash type, which has advantages over other techniques in the field of chemiluminescence immunoassay. The intensity of the chemiluminescence reaches the maximum after 0.4 s, the half-life is 0.9 s, and the luminescence basically ends within 2 s, which can realize rapid detection. Acridine ester has high quantum yield and high chemiluminescence efficiency, It's usually five times or more than luminol.   3. Acridine ester luminescence system has few interference factors, low background and high signal-to-noise ratio. The characteristic of acridine esters from the perspective of luminescence mechanism is that the non luminescent substituents attached to the acridine ring are separated from the acridine ring before the formation of electron excited state intermediates, that is, the non luminescent part is separated from the luminescent part, so the luminescence efficiency is not affected by the substituent structure.   In addition, acridine ester has small molecular weight, easy to bind with protein through chemical bond (i.e. labeling), little influence on the conformation of antibody after conjugation (thus ensuring its good reactivity in subsequent immune detection), and good stability of conjugates.   Desheng has six kinds of acridine ester products with different groups: acridine ester dmae-nhs, acridine ester nsp-dmae-nhs, acridine salt nsp-sa-nhs, acridine salt nsp-sa-nhs, acridine hydrazide nsp-sa-adh, acridine ester me-dmae-nhs.   Desheng is an old chemical reagent company specializing in the R & D and production of blood test reagents. Up to now, it has 14 years of R & D and production experience. Luminescent reagent substrate has always been the top priority of the company's research. Acridine ester series has high luminous efficiency and belongs to direct luminescence. Need acridine ester series of business friends can directly contact 18971041571 (wechat number), looking forward to your call!

Carbomer 980 is a commonly used carbomer material. Carbomer is a high molecular polymer of acrylic acid allyl sucrose or pentaerythritol allyl ether. It is usually loose white micro acidic powder. It can produce high efficiency thickening under low dosage, thus producing wide viscosity range and rheological properties of emulsion, cream, gel and transdermal preparation.   The properties of different carbomer are slightly different, and different models represent different viscosities, which are called short rheological or long rheological. For example, Carbomer 940 has short rheological properties, and its viscosity reaches 63000 MPa. S at 0.5%, which is suitable for the application For high viscosity products, Carbopol 941 has long rheological properties, reaching a viscosity of 7500mpa. S at 0.5% Suitable for low viscosity products, Carbomer's corresponding models are ion resistant and ion resistant.   Carbomer 980 has good thickening property, high transparency, high viscosity and strong suspension ability. Carbomer 980 is widely used in daily chemical products, such as skin care emulsion, cream, transparent gel products, transparent skin care gel, hair styling gel, shampoo, shower gel and disinfectant free gel.     Dissolution method in water or homogenizer: First, add carbomer into deionized water according to the actual production requirements to dissolve it without stirring. After the carbomer absorbs water naturally, take it as the criterion that there is no white powder on the surface and no white agglomerate in the solution. Stir it evenly, and then add neutralizing agent (carbomer: neutralizing agent = 1:1) 1. Kapo 5% neutralizing agent solution = 1.4.5) adjust the pH value at about 7 to reach thickening state. Use a round tool or a disperser and stir evenly at low speed. By adding homogenizer, the white group can not be seen in the actual production proportion, and neutralizer is added to form the gel, then the vacuum emulsification pot is used to remove the air from the gel.   matters needing attention: 1. If the solution is dissolved by common methods, Carbomer can not be stirred in water, while stirring, Carbomer will form a white water ball, and bubbles are difficult to eliminate after neutralization; 2. After carbomer neutralization, long time stirring or high shear stirring will cause viscosity loss; 3. The existence of electrolyte will reduce the thickening efficiency of kapok resin; 4. Long term UV irradiation can reduce the viscosity of kapok resin.   Desheng Technology specializes in acrylic series of blood vessel separation adhesive, Carbomer 980, caps, mops and other surfactants and biological buffers. Customers are welcome to consult.

The full name of Tris is tris(hydroxymethyl)aminomethane, which can also be called tromethamine, CAS77-86-1. Tris (Tris) is often used as a biological buffer, and TAE and TBE buffers (used for the dissolution of nucleic acids) commonly used in biochemical experiments are required. What other applications does it have besides this? Let's take a look next.   In the field of medicine, Tris is not only an important pharmaceutical intermediate, it is also a kind of drug itself, suitable for metabolic acidemia, respiratory acidemia and other diseases, especially in hyperuricemia. In terms of treatment, the effect is relatively satisfactory.   Hyperuricemia is divided into two categories: primary hyperuricemia and secondary hyperuricemia, mainly due to excessive sources of uric acid or (and) insufficient excretion. Under normal circumstances, if the two fasting blood on the same day is not the same, the uric acid level of men is higher than 420μmol/L, and women's uric acid level is higher than 360μmol/L, which is hyperuricemia. According to social understanding, the age of high incidence of hyperuricemia is middle-aged and old men and postmenopausal women. In recent years, there is a trend of getting younger.     The current prevention and treatment strategies for hyperuricemia include reducing intake, inhibiting endogenous synthesis, and promoting renal excretion. The promotion of renal excretion is mainly achieved by alkalizing urine and inhibiting uric acid transporters. Inorganic metal cations in urine such as Ca2+, Mg2+, NH4+, etc. can inhibit the dissolution and excretion of uric acid, and alkaline factors can promote the dissolution of uric acid, so tris and sodium bicarbonate are often used in this process. But in the function of promoting uric acid dissolution and excretion, the effect of tris is better than sodium bicarbonate.   After taking sodium bicarbonate, the increased cation Na+ will further resist HCO3- alkalizing urine and blood, and promote the dissolution and excretion of uric acid. Sodium bicarbonate will even reduce the solubility of uric acid after a certain dose. However, sodium bicarbonate can be produced endogenously. There is sodium bicarbonate in the human body, and there are large fluctuations in the urine, so the concentration of sodium bicarbonate is difficult to control. If the amount of sodium bicarbonate is small, uric acid will be excreted. The effect is not good. If you take more, it may increase the deposition of uric acid in the urinary system, and the intake of sodium salt will also increase the burden on the cardiovascular and kidneys.   Tris is an organic amino base. There are no inorganic cations, which can avoid or weaken the inhibitory effect of inorganic cations. In addition, Tris is a foreign substance and the body will not produce tris. As a methane substance, the concentration of tris in the body is better controlled than sodium bicarbonate in the process of medication. In summary, it can be concluded that tris is better than sodium bicarbonate in promoting the dissolution of uric acid.   Desheng has been doing in-depth research on biological buffers for a long time, and can provide high-quality raw materials such as Tris, Bicine, Hepes, etc. Desheng adheres to the company philosophy of "Quality First, Technology Leading", and provides customers with high-quality products.

N-Tris(Hydroxymethyl)Methyl-3-Aminopropanesulfonic Acid (TAPS Buffer) is a zwitterionic buffer widely used in the fields of biochemistry and molecular biology, CAS number: 29915-38-6, and the pH buffer range of TAPS is 7.7 -9.1, soluble in water (25g/50ml).   In clinical diagnosis, TAPS Buffer is used as a biological buffer in biochemical diagnostic kits. So what factors can affect the yield and purity of TAPS Buffer? The reaction time, different alcohol solvents and the recycling of reaction solvents have a certain degree of influence on various types of biological buffers, and the same is true for TAPS Buffer. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, and the yield will decrease slightly; for the investigated ethanol, n-propanol, isopropanol and n-butanol reaction solvents, n-butanol As the reaction solvent, the yield and purity of TAPS are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS Buffer.   TAPS Buffer reagent TAPS Buffer is a commonly used buffer system in DNA screening systems, and also as a buffer component of RNA samples. It is suitable for electron transport and phosphorylation studies of chloroplast thin-layer preparations, and protects oxygenated hemoglobin from oxidation during freeze-drying. Methemoglobin can also be used as a background electrolyte for protein microanalysis in capillary zone electrophoresis.   The quality and service of TAPS Buffer produced and developed by Desheng are guaranteed. The manufacturer provides stable supply and can provide one-stop purchase of raw materials, saving your purchase time and cost. Desheng owns biological Buffer TAPS Buffer has complete qualifications in all aspects, including test reports and so on.

The commonly used biological buffers on the market: Tris, Tris HCl, bicine, caps, mops, taps, EPPs, HEPES, PEP, pipes, MOPSO, etc. why do we choose bicine? First, let's look at what bicine is? What are its special advantages?   Bicine is a white crystalline powder with the chemical name of N, N-bis (2-hydroxyethyl) glycine (CAS: 150-25-4) and English name of N, N-bis (2-hydroxyethyl) glycine. Bicine white crystalline powder It is soluble in water and insoluble in acetone, DMF, DMSO, DMAC and other organic solvents. N. N-dihydroxyethyl glycine is a pH buffer suitable for biochemical and medical research. The pH buffer range is 7.6-9.0, and the pKa value is 8.3 at 25 ℃. This property makes it often used as a buffer solution for capillary electrophoresis. Its molecular structure is similar to glycine.   It is a widely used buffer. It is not only used to prepare stable substrate solution for the determination of guaninase, but also used as electrophoresis buffer. The working concentration is 3-100ml. Electrophoretic buffer is an important component of the gel electrophoresis system of nucleic acid and protein. It is also a conductor in the electrophoresis field and a necessary condition for maintaining the constant pH value of the electrophoresis system.   Buffer solution refers to the mixed solution composed of weak acid and its salt, weak base and its salt, which can offset and reduce the influence of strong acid or strong base on the pH value of solution to a certain extent, so as to keep the pH value of solution relatively stable. In biochemical research, buffer solution is often used to maintain the pH of the experimental system.   The change of pH value of solution system in research work often directly affects the effect of research work. If the pH value of the "extraction enzyme" experimental system changes or greatly changes, the enzyme activity will decline or even completely lose. Therefore, the preparation of buffer solution is an indispensable key step.   Buffer solution is an important concept in Inorganic Chemistry and analytical chemistry. Buffer solution is a solution that can maintain the relative stability of pH. In a certain range, the pH value does not change significantly due to dilution or addition of a small amount of acid or base. The buffer solution has different pH value and buffer capacity according to the amount of conjugated acid-base pairs and their substances.   Desheng technology will continue to develop and research advanced biochemical reagents to meet the needs of the development of global medical laboratory technology and strive for human health. The company customized a variety of ready to use solution, welcome to consult, pH can be customized.

Dark and light, often more favored by human light, invisible things always give people a sense of unknown, in order to more intuitive check whether the detection material contains a substance, chemiluminescence series reagents came into being. Among them, luminol is widely used, and acridine ester is the best choice for many kit manufacturers in recent years.   Conventional acridine ester series include acridine ester dmae-nhs, nsp-dmae-nhs, nsp-sa-nhs, nsp-sa-nhs, acridine hydrazide nsp-sa-adh, me-dmae-nhs, while cas194357-64-7 acridine ester nsp-dmae-nhs has been modified in structure, increased steric hindrance, enhanced anti hydrolysis, modified in hydrophilicity, introduced propanesulfonic acid inner salt, increased its solubility in water, and made it compatible with antibody, anti-dmae-nhs The markers formed by nucleic acid are water-soluble, and the subsequent luminescence detection can be carried out in water.   Chemiluminescence reagent acridine ester Acridine ester nsp-dmae-nhs is naturally welcomed by the market because of its distinctive characteristics. However, the market is full of variety. Traders and middlemen are confused. Even if they find the right manufacturer, the quality of the product can not be judged at the beginning. Here to recommend a more reliable acridine ester manufacturer - Hubei xindesheng Material Technology Co., Ltd.   Hubei new Desheng materials was established in 2017, its predecessor was Wuhan Desheng Biochemical Technology Co., Ltd., and it began to establish its own R & D team in 2005. In the blood detection industry has a great influence, and as a chemiluminescence reagent acridine ester has been one of Desheng's more important research projects.   Dedicated to acridine ester research team, laboratory professional production, supporting production and testing equipment, thoughtful service, all make Desheng acridine ester nsp-dmae-nhs better than other brands. Desheng acridine ester products have 6 different groups, for all kinds of different needs of enterprises to choose, choose high-quality acridine ester, choose Hubei xindesheng Materials Co., Ltd!

Tops sodium salt is a kind of white crystal powder, which is also called tops sodium salt by many people. It can be used for the colorimetric determination of uric acid and cholesterol. It is also a common color reagent in biochemical test kit. Packaging of tops sodium salt reagent There was once a customer who did biochemical research in the laboratory. After a friend's introduction, he came to our company and bought tops sodium salt products. At the beginning, the customer bought only a small amount of products. He was also trying to see how the products were. However, when he received the package, he was surprised to find that Desheng's service was excellent. A small brown bottle was packed with three layers inside and outside, The carton on the outermost layer is the thickest, and the logo of Desheng is printed on it. You should know that this customer not only bought the product for trial in Desheng, but also bought the same product from other manufacturers. Finally, the comparison shows that Desheng's service is the most intimate, the delivery speed is the fastest, and the product quality is the best, There is no doubt that this customer finally became Desheng's long-term buyback customer. Every month, we have to order more than one kilogram of tops sodium salt reagent in our company. The development of the enterprise cannot do without details. Doing well in details and good service will get more customers' recognition.   Tops sodium salt reagent research team Desheng produces and develops more than ten kinds of color reagents, each of which has a professional scientific research team of more than ten people. Top sodium salt has 11 scientific research teams, including 1 Professor, 4 associate professors, 2 associate researchers, 1 doctoral candidate and 3 master candidates.   Only an enterprise focusing on product research and development can give customers the greatest degree of guarantee. The most important factor for any enterprise to become bigger and stronger is the quality. No one has the same color reagent. The color reagent products developed by Desheng are impeccable in terms of purity, sensitivity, stability and appearance, The quality inspection department strictly controls all links of the raw materials of color reagent from warehousing to production, and also provides substantial guarantee for products. At present, the company has successfully become the first batch of science and technology enterprises entering 2020, and Desheng will become more and more powerful in the future.   Desheng has a wide range of chromogenic reagents, and its best-selling products include toos, Maos, tops, ADOS, ADPS, Alps, Daos, hdaos, MADB. Not only it has good water solubility and no difference in approval documents, many products also have patent certificates, which are well received in the industry and have 50 + loyal customers. At present, tops has sufficient reagent stocks. Manufacturers are welcome to inquire and purchase. If necessary, they can also come to our company for field investigation.

The history of immunology began with the study of Microbiology, established in the 18th century, and entered the classic development period from the 19th century to the mid-20th century. During this period, people's understanding of immune function entered the period of scientific experiment from the observation of human body phenomenon.   From the early to the middle of the 20th century, it entered the period of modern immunology. From the middle of the 20th century, it really entered the period of modern immunology. The detection of modern immunology has gone through the following processes.   The specific reaction of antigen and antibody is used to detect, and isotope, enzyme and chemiluminescence are used to amplify and display the detection signal. It is often used to detect protein, hormone and other trace substances. Immunodiagnosis plays a very important role in clinical diagnosis. The common immunotechnologies include radioimmunoassay, enzyme-linked immunosorbent assay, chemiluminescence, electrochemiluminescence and chemiluminescence of magnetic nanoparticles.     1. Principles of radioimmunoassay The radiolabeled antigen and unlabeled antigen (to be tested) were competitively combined with insufficient specific antibodies, and the amount of unlabeled antigen was obtained by separating and measuring the radioactivity after reaction.   2. Principle of enzyme linked immunosorbent assay Referred to as ELISA, its center is to let the antibody and enzyme complex binding, and then through the color to detect. The antigen or antibody can be bound to the surface of a solid carrier and keep its immune activity. To make an antigen or antibody linked with an enzyme to form an enzyme-linked antigen or antibody. The enzyme-linked antigen or antibody retains both its immune activity and the activity of the enzyme. Because of the high catalytic frequency of enzyme, it can greatly enlarge the reaction effect and make the determination method reach high sensitivity. ELISA can be used to determine antigen and antibody.   3. Principle of chemiluminescence Technology The free energy released by the chemical reaction is used to excite the intermediate and make it return to the ground state from the excited state. When the intermediate returns to the ground state from the excited state, it will release equal level photons. Chemiluminescence has the specificity of fluorescence, at the same time, it does not need to excite luminescence, which avoids the influence of stray light of excitation light in fluorescence analysis, so as to improve the sensitivity, and avoid the environmental pollution and health hazards caused by radiation analysis. It is a very excellent quantitative analysis method.   4. Principle of electrochemiluminescence Technology Electrochemiluminescence (ECL) is the result of the participation of electric field in chemiluminescence, which refers to the electrochemical reaction by applying a certain voltage: the TPA on the electrode surface releases electrons, and then releases protons to become free radical TPA *, at the same time, the divalent Ru (bpy) 3] 2 + releases electrons to become trivalent Ru (bpy) 3 + 3 +. There are still divalent Ru (bpy) 3] 2 + and tPA in the reaction system, so that the electrochemical reaction on the electrode surface can continue. In this way, the whole reaction process can be continuously cycled, and the detection signal is continuously amplified, so the detection sensitivity is greatly improved, so the ECL determination has the characteristics of high sensitivity.   5. Principle of chemiluminescence immunoassay with magnetic particles It is a new analytical method that combines magnetic separation technology, chemiluminescence technology and immunoassay technology. The technology makes full use of the rapidity and automaticity of magnetic separation technology, the high sensitivity of chemiluminescence technology and the specificity of immunoassay. It plays an irreplaceable role in the field of biological analysis. At present, magnetic particle chemiluminescence immunoassay has been used in tubular chemiluminescence immunoassay and electrochemiluminescence immunoassay.

Taps name n-tri (hydroxymethyl) methyl-3-aminopropane sulfonic acid, CAS No. 29915-38-6, molecular formula c7h17no6s, from the appearance, belongs to white crystal, its content is very high, more than 99%. Trimethylolmethylamino propanesulfonic acid (TAPs) is also a kind of biological buffer. It has good water solubility. The concentration is usually 25g / 50ml. The solution is colorless and transparent, and very clear. The pH value is between 7.7 and 9.1, and the pKa value is 8.4.   Taps is a kind of zwitterionic buffer widely used in biochemistry and molecular biology. It can be used not only as a buffer system in DNA screening system, but also as a buffer component of RNA sample; It can also be used as an important pH stabilizing reagent, which is usually mixed with weak acid and its conjugated base to obtain appropriate pH value.   He is used for electron transfer and phosphorylation of chloroplast thin layer preparation; During the freeze-drying process, oxyhemoglobin can be protected from oxidation to methemoglobin; It can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.   In biochemical experiment projects, taps reacts with most organisms under neutral conditions. The pH value should be between 6 and 8, and the effective buffer range of buffer should also be between 6 and 8. Moreover, it is necessary to ensure that the pH form of buffer reagent cannot chelate with some metal chemical ions. Taps, a buffer used in biochemical detection and molecular diagnosis, has high requirements for reagent purity and impurity ion content.   In the field of clinical diagnosis, taps is also commonly used as a biological buffer. It is usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA / RNA extraction kits.   Desheng is skilled in technology. Taps buffer is made of high purity and high quality taps. After strict filtration, sterilization and quality testing, it can be directly added to the culture medium. It should be noted that taps buffer, buffer range is ph7.7-9.1, PKA (ion concentration set) is 8.4.   Taps buffer is directly added to the sterile medium or added to the instrument to filter and sterilize the medium. Desheng technology has done a lot of research and improvement in this aspect, and many kinds of biological buffers produced by the company have been recognized by a large number of biochemical testing enterprises and pharmaceutical equipment enterprises.