China Wuhan Desheng Biochemical Technology Co., Ltd Company News

How to measure heparin sodium As an anticoagulant substance, heparin sodium usually has a greater anticoagulant effect after being formulated into a solution, but sometimes it cannot be added at will, and heparin sodium needs to be determined according to the calculation formula to ensure the accuracy of dosage sex. According to the experimental requirements, the adsorption rate, absorbance, solution concentration and titer of heparin sodium are generally measured. The following will focus on introducing it to you.   1. Determination of the adsorption rate of heparin sodium The potency of the heparin sodium solution after adsorption is detected after a certain interval of time, and the total potency remaining in the solution is calculated at the same time, and the adsorption capacity is characterized by the total potency remaining. The titer of heparin sodium solution determined before adsorption was set as D0, and the titer of the experimental solution determined after adsorption was set as D1. (D0-D1) is the D value of resin-adsorbed heparin sodium. The average value of three parallel experiments is taken, and the determination formula is M=(D0-D1)/D0.   2. Determination of absorbance of heparin sodium Determination of the absorbance value of heparin sodium can adopt ultraviolet spectrophotometer, when the monochromatic light radiation passes through the measured substance solution, it is directly proportional to the amount absorbed by the substance and the thickness and concentration of the liquid layer, and its determination formula is A=lg(1 /T)=E×C×L.   3. Determination of the concentration of heparin sodium solution Prepare the test solution and the control sample solution during the preparation process. The amount of the tested components contained in the reference solution should be 100% ± 10%, and the reagents used should be consistent. Measure the test solution and the reference substance at the specified wavelength. After measuring the absorbance of the solution, the concentration of the solution can be calculated according to the determination formula.   4. Determination of Heparin Sodium Potency Usually, the sheep plasma method is used for determination. The water temperature is adjusted to about 37 degrees, and the frozen sheep plasma is taken out, melted and filtered, and set aside. Then two groups of test tubes with different ul were taken respectively, and 8% sodium citrate solution, 0.25% calcium chloride and filtered sheep plasma were added into the tubes. Cover the test tube, invert and mix slowly 3-4 times to make the inner wall wet, put it vertically in a water bath with a constant temperature of 37°C, take out the test tube after 1 hour, check the degree of coagulation, and record and calculate.   Desheng is a manufacturer of heparin sodium in China. It can provide professional testing reports. The potency of heparin sodium for different purposes can be customized according to customer requirements, and it is equipped with one-to-one after-sales service. Any problems will be solved immediately to ensure customer purchase experience. . If you have relevant needs, welcome to click on the website or call for details!

How to use heparin sodium       As a naturally occurring anticoagulant, heparin sodium is hidden in the liver organs of mammals, such as cattle, sheep and pigs, and then prepared into anticoagulants in vitro, which is conducive to reducing the blood coagulation ability and preventing the formation of harmful clots in blood vessels, so it is favored by various medical institutions, and its application field is gradually extensive.       However, it should be noted that heparin sodium has its own use methods and requirements. If you do not know how to use it, it is likely to lead to poor anticoagulation effect and deviation in the test results. So let's introduce how to use heparin sodium in detail. You can also pay attention to it when using it.       1. As an anticoagulant for blood in vitro, crude heparin cannot be used directly. The refined heparin sodium needs to be used to ensure the purity of the appearance color. It is dissolved in water to prepare a solution and then directly sprayed on the inner wall of the test tube. The conventional dosage of 1 ml of blood corresponds to 20 IU of heparin sodium.       2. Use after adding solvent to test tube γ Irradiation sterilization is carried out by irradiation with a dose of 8-25 kGy. Generally, the irradiation dose can also be determined according to the initial number of colonies.       3. Heparin sodium can be used at the same time with serum separation gel, and its anticoagulation and centrifugation effects can reach the best state, which is conducive to more accurate detection results.       4. When using heparin sodium anticoagulant tube, the blood sample should be inverted and mixed for at least 8 times as soon as possible after being collected, especially when the ambient temperature of blood collection is too high, if the mixing is not timely, half of the coagulation and half of the non-coagulation phenomenon will occur, pay attention to the use of shaking should not be too strong, just gently inverted.       5. When heparin sodium is used for anticoagulation, because it can participate in cell metabolism, the amount of heparin sodium used will interfere with the test results, so the amount of heparin sodium used should fully ensure the complete anticoagulation of the sample, so as to ensure the accuracy of the value.       The above five aspects briefly introduce how to use heparin sodium and the points for attention. As an anticoagulant in vitro, its requirements must be stricter. Knowing how to use it can effectively avoid problems in the results.       Desheng, as an old brand manufacturer of blood collection additives in China, has not only rich experience and strong strength, but also has always implemented the standard of "strict control" in product quality, and domestic enterprises that have cooperated with it have been praised in succession. At the same time, because it is directly supplied by the manufacturer, a large independent warehouse has been set up to meet a large number of customers' needs, and there is no shortage of goods. If you have any intention, please click the website or call for details!

What is the price of EDTA tri-potassium?       In recent years, with the progress of medicine, in order to save time and improve efficiency, many hospitals will generally use some reagents to assist in the detection, such as EDTA tri-potassium, which is frequently used in vacuum blood collection as an anticoagulant in vitro. Due to the increase in demand, the price of EDTA tri-potassium produced by various manufacturers in the market is rising. Many customers will pay special attention to the price. So, let's introduce it to you in detail.       Desheng has also received many calls from customers recently, all of which are sighing that "our price is cheaper than that of other manufacturers" and "the delivery speed is also very efficient". However, it is not that the price of Desheng Desheng has changed, but that of other manufacturers in the market has increased.       As the manufacturer of EDTA potassium trioxide, Desheng has always followed the principle of customer first in terms of price. As long as the price range of raw materials is not large, the price we give customers will not change easily. If it is a long-term customer, it will also enjoy additional preferential prices. So many people will basically choose Desheng after asking the price in the market. In addition, the company will provide sample tests to save customers the time to choose to buy.       So, what is the price of EDTA tri-potassium? In fact, this may ask different manufacturers, and the answer is completely different. Because there are many manufacturers of EDTA tri-potassium, but few of them produce dihydrate, and if equipped with professional technical teams, it is rare. So there will be many "manufacturers" with slogans on the market, but they are actually traders. It is not that the traders are not good, but that the traders will make different quotations on cost accounting. Most of the prices will be on the high side, and the manufacturers will have some advantages and lower prices.       How much is the price of Tri-potassium EDTA in Desheng? In fact, Desheng advocates batch quotation, just like the price of buying a thing is different from the price of bulk purchase. The price will be high if you order a small amount, but the goods above the ton level will give customers a favorable price. Of course, it should be noted that the products under the same level, EDTA tripotassium, have pharmaceutical level and industrial level, and the prices of these two levels are also different. When you are consulting, you should express your needs clearly so that you can get more accurate quotations. If you have any need for EDTA potassium salt, please click the website or call to purchase!

Dipotassium ethylenediaminetetraacetic acid, also known as EDTA-K2, is often used as an anticoagulant and added to a vacuum blood collection tube, usually a test tube with a purple cap. In addition, some nucleic acid detection tubes use EDTA-K2 as an anticoagulant, mainly because EDTA has an affinity with calcium ions in the blood and complexes, thereby inhibiting blood coagulation. However, in addition to being used for blood collection tubes, it is also widely used and is often used in detergents, liquid soaps, shampoos, and antidotes.   The dipotassium EDTA produced by Desheng is a white crystalline powder, odorless and easily soluble in water. Its product performance is closely related to the production process. During the entire production operation, personnel must be specially trained, strictly abide by the operating regulations, and pay special attention to the following issues during production: 1. Operators wear masks, work clothes, gloves and safety glasses during the production process to ensure their own safety while keeping the product does not contain impurities. 2. Smoking is strictly prohibited in the production site, keep away from all kinds of fire and heat sources. 3. Avoid contact with oxidants and place them in the same site. 4. Strictly abide by the production index of dipotassium EDTA, and meet the main content, pH value, solubility and other data requirements of the product. 5. After production , store it in a cool and ventilated warehouse, do not mix it with oxidants, and equip corresponding utensils to contain the leakage. 6. When sub packaging, handle with care to prevent damage to packaging and containers and avoid quality problems. 7. The quality department will conduct quality inspection on the same batch of goods, and only after passing the inspection can they be packaged and sold.   Dipotassium EDTA, as an important integrator, can chelate metal ions. It can be used in blood collection tubes to inhibit blood coagulation, and it can also be used in the industry to prepare metal polishing agents, deodorants, and surfaces. Dipotassium EDTA occupies a pivotal position in the market, attracts many merchants producing with different quality. So it makes buyers not easy to find a good manufacturer.   Desheng Biochemical is recommended here, which is an established manufacturer with a professional team of production,sales, and one-stop service. At present, various products of blood collection tube reagents produced through strict control have been affirmed and praised by home and abroad.If you have needs in this regard, you can click the website to consult details!

As one of the important raw materials in the field of biochemistry, heparin plays its role in many aspects, such as the preparation of medicine, testing, cosmetics, etc. Among them, the most widely used is used as an in vitro blood anticoagulant, whether it is heparin sodium or heparin Lithium is an anticoagulant reagent favored by medical institutions.   Although heparin sodium and heparin lithium are both used as in vitro blood anticoagulants, they are both white powder in appearance, have no peculiar smell, and are soluble in water, but this does not mean that they are exactly the same, and there are still many differences. Let me introduce the difference between heparin sodium and heparin lithium.   1. The difference of binding ions Even though heparin sodium and heparin lithium exist in another form of heparin, the ionic structure is very different, which makes heparin sodium and heparin lithium look the same in appearance, but they are essentially different , and their degree of dissociation is also different. Generally speaking, lithium heparin is replaced from sodium heparin. Second, the difference in the amount of additives in blood collection tubes   2. heparin and lithium heparin are widely used in vacuum blood collection tubes, but they are not added blindly. They need to be added according to industry standards. There is a fundamental difference in the amount of heparin added. The standard dosage range of heparin lithium in the industry is 9.41-281U unit/per milliliter, while heparin sodium is 121U~301U per milliliter of blood. Sodium is 151U-201U.   3. Differences in white blood cell count results In the detection experiment, it can be known that heparin sodium and heparin lithium have different results on the white blood cell count of the same specimen, and the law of heparin sodium is more obvious, and it can change with time. Hours later, it gradually decreased, and the platelets also gradually decreased. However, heparin lithium is different. The detected white blood cell count and platelet value drop not much, and are relatively slow and stable. In the presentation of the results, the results of heparin sodium and EDTA dipotassium may be similar.   From the above three aspects, we can see the difference between the two, and you should not confuse them in the future. However, in addition to the two anticoagulants of heparin sodium and heparin lithium, the commonly used anticoagulant is EDTA potassium salt , sodium citrate, etc. As an established manufacturer in China, Desheng has a complete range of anticoagulant products and its quality is widely recognized. At present, Desheng has cooperated with many foreign companies. If you have relevant needs, please click the website for consultation!

What effect does heparin sodium have？       As a natural ideal anticoagulant, heparin is stored in liver organs of mammals, especially in pig intestinal mucosa, and then extracted into desired heparin products, such as heparin sodium. Speaking of its role, because of its special performance, it can be widely used in various fields, such as medicine and cosmetics. The following is a specific introduction to the role of heparin sodium in various fields.       1、 Used for vacuum blood collection       As an anticoagulant, heparin sodium can be added to the vacuum tube as an extracorporeal anticoagulant. When blood collection is completed, it plays a role in preventing blood coagulation within a certain period of time, which not only brings convenience to medical personnel, but also improves the overall blood detection efficiency, which is also a preferred method in many medical institutions.       2、 For anticoagulants       In addition to being used as an external anticoagulant, heparin sodium can also be used as an anticoagulant in vivo. However, this kind of heparin sodium has a higher level and is generally used as an anticoagulant to treat thrombotic or cardiovascular and cerebrovascular diseases. In addition, injection grade drugs are usually suitable for patients who need rapid anticoagulation in clinical emergency and play a role in preventing major vascular blockages.       3、 For cosmetics       Used in cosmetics, it appeared in the form of cream in the early stage, which can play a role in recovering skin lesions, and later it gradually extended to the cosmetics field. It has been found that when used in cosmetic raw materials, it can play the role of anti inflammation, anti allergy, improving skin permeability, moistening and promoting cell proliferation, as well as good health care and maintenance.       Heparin sodium has a wide range of functions. As a manufacturer of heparin sodium in China, Desheng has always produced heparin sodium of non injection grade, which is mainly used as an extracorporeal blood anticoagulant in blood collection vessels. This needs to be clear. In addition, Desheng has always been pursuing excellence and excellence in the quality of heparin sodium products, and strict requirements and specifications are required for various indicators of the products, so that major enterprises can purchase with confidence and specify specific needs when purchasing, so that customer service can provide you with more accurate pricing and services. Welcome to purchase!

  When we enter the hospital to check the disease, the doctor will first conduct blood tests, and then judge the condition according to the blood test results. As a routine examination in medical institutions, it not only depends on supporting analytical instruments, but also needs to be supplemented by some additives to improve the overall efficiency. Heparin sodium is a common anticoagulant in blood analysis. Because of its good anticoagulant effect and no effect on blood cells, it is widely used in clinical detection.   Heparin is mostly extracted from pig intestinal mucosa, and then purified, filtered and dried to obtain a powder like substance. When using, the powder needs to be prepared into a solution, and then sprayed on the inner wall of the test tube, which can play an anticoagulant effect. Then, how to dissolve the solution prepared from powder? The following is a detailed introduction to the dissolution methods and steps of heparin sodium salt. You can refer to the completion of dissolution.   Dissolution method and steps:   1. Prepare the balance, select the required volumetric flask, beaker and deionized water according to the requirements, and then weigh them. Weigh the heparin sodium powder determined in advance, and pay attention to the accuracy of 1/10000. Then pour the powder into the volumetric flask to ensure that the volumetric flask is clean and free of impurities.   2. Use distilled water or deionized water for dissolution. First add a certain amount of deionized water into the beaker, slowly stir with a glass rod while adding water, and wait for full dissolution. During dissolution, pay attention to the control of water consumption, and leave a certain volume for subsequent cleaning containers, otherwise the concentration of heparin will decrease.   3. After the heparin sodium is completely dissolved, the volume shall be fixed, the line of sight shall be kept at the same level with the scale line of the volumetric flask, and the deionized water shall be slowly added along the neck wall of the volumetric flask with the washing flask until the scale line is reached.   4. Evenly mix, press the cork with your hand until the volumetric flask is sealed, hold the bottom, and turn it upside down slowly for 5-8 times.   The above is the dissolution method of heparin sodium salt, which is mainly to use deionized water for dissolution, and then prepare a solution with a certain concentration and content to add to the blood collection tube. Pay attention to the prepared solution, and do not store it for a long time to avoid breeding bacteria and affecting the experimental effect.    Desheng Biochemical is a Chinese manufacturer specializing in the production of heparin sodium salt. The process is stable and does not contain any impurities. It is very popular in the domestic market. Now it is expanding abroad, and has gradually been sold to many countries, and has reached the intention of long-term cooperation. If you have this demand, you can click the official website for details!

What is the composition of serum separating gel?       Serum separating gel is an additive often used in vacuum blood collection vessels to quickly and accurately separate blood clots and serum. It is widely used in major hospitals due to its simple, clean, safe, accurate and reliable operation. What is the composition of serum separating gel? Blood samples are often sensitive to PH. What is the pH value of the gel? In fact, the serum separating gel is composed of macromolecular compounds. At present, the separating gel produced by Hubei Xindesheng has many systems, and the difference of systems determines the difference of composition.  

  Why are EDTA K2 and EDTA K3 anticoagulants of choice in routine hematology？       In the routine blood testing, various medical institutions mostly use vascular additives and supporting equipment and instruments for analysis, more common additives are anticoagulant, coagulant promoting agent, serum separation glue, etc. In terms of market application, there are many types of anticoagulant products, such as EDTA K2 and EDTA K3, which are widely used. Why are these two reagents so popular? The following details for you to introduce.       What are EDTA K2 and EDTA K3?     EDTA K2 is a strong anticoagulant commonly used to store blood in blood collection tubes, which can stop blood clotting for a certain amount of time. Its mechanism is because of its chelation property, after full contact with blood in test tubes, it can achieve the purpose of trapping metal ions, thus reducing reactivity at the root and preventing blood clotting. Speaking of EDTA K3, in fact, it is another form of EDTA K2. Its performance and action mechanism are the same, that is, it has one more potassium ion than EDTA K2 in the number of ions.       Why are EDTA K2 and EDTA K3 commonly used anticoagulants?     The main reasons for the use of EDTA K2 and EDTA K3 as anticoagulants in routine blood tests are:     1. Both of them are anticoagulant by chelating potassium ions;     2, both play a role in preventing the blood stored in the tube from clotting, both can be achieved by chelating the calcium ions of the blood;     3, Both are approved by the International Blood Committee in vitro blood anticoagulants, suitable for common routine blood test analysis, thus complete blood count;       Use points when selecting EDTA K2 and EDTA K3     1. First of all, it is important to determine the blood collection volume and the dosage of EDTA K2 or EDTA K3. Usually, the blood collection volume is 2mL and the anticoagulant dosage is 20uL.     2. Since the produced EDTA K2 and EDTA K3 are in powder form, they need to be configured as solution. It should be noted that the storage condition of the solvent should be kept at 0-4℃ and the storage time should not exceed one week.     3. When using EDTA K2 or EDTA K3 anticoagulant tubes for blood collection, it should be noted that the anticoagulation of EDTA salt is not irreversible anticoagulation, and the detection should be completed within 24 hours after blood collection.     Desheng Biochem is the main supplier of vascular additives, producing a series of products, which can meet the public one-stop shopping needs. And product quality by professional R & D personnel layer by layer check, sampling and repeated testing, testing qualified, can be sold to countries. If you have related needs, please click on the website for details!

    Is the blood anticoagulant function of CAS25102-12-9 EDTA 2K continuous?       EDTA 2K, known as dipotassium ethylenediamine tetraacetic acid in Chinese, CAS No. 25102-12-9, is an important anticoagulant added to vacuum collection vessels, compared to other types of anticoagulants, such as: Heparin, sodium citrate, potassium oxalate, etc., are used more frequently, so here to give you a detailed introduction, to help you understand the details of it, to help use in clinical testing.       EDTA 2K Blood anticoagulant Time     As a widely used anticoagulant, EDTA 2K is sufficient to demonstrate its good anticoagulant effect and stable performance. However, it should be noted that its blood anticoagulant function is not continuous, which means its anticoagulant time is limited. Because EDTA is a non-irreversible anticoagulant, medical personnel usually complete the test within 24 hours after blood collection to avoid errors in test results.       Type of sampling vessel for EDTA 2K applications     The amount of anticoagulant added to different types of anticoagulant tubes varies. EDTA 2K is usually added to a vacuum tube used to test blood routine. The tube cap is purple. In addition, it can also be used in nucleic acid detection tubes. The main mechanism is that EDTA complexing magnesium ions, so that it will have an inhibitory effect on nucleases requiring the presence of magnesium ions to play a role, and can prevent nucleic acids from being degraded by nucleases.       The amount of EDTA 2K     The dosage of EDTA 2K in the collection vessel is determined by the amount of blood collected. The content of EDTA 2K per ml of blood ranges from 1.5 to 2.2mg. The median value of EdTA 2K is 1.8mg/ml when preparing reagents. It should be noted that the dosage of EDTA 2K is separate from the amount of heparin anticoagulant. The dosage of EDTA 2K is by weight and heparin is by titer. It should not be confused.       EDTA 2K solution preparation     EDTA 2K is white crystalline powder, which should be prepared into solution in the preparation of anticoagulant tube. The concentration of the solution should not be too low or too high. When increasing the dosage of EDTA 2K, the concentration should be increased first and then the spraying amount. The reason why the concentration should not be too high is that when the temperature drops, the solubility will also change, resulting in the precipitation of EDTA 2K crystals, sometimes causing the risk of hemolysis. Usually, 20 microliters of EDTA 2K solution is added to 2mL or 3mL of blood collection.       Desheng Biochemical focuses on the research, development, production and sales of vascular additives. As a source manufacturer, Desheng Biochemical is not only experienced, but also supervised and controlled by professionals. There is no middleman profit margin, and there is no inferior products. At present, the series of vascular collection products produced, such as serum separation glue, heparin sodium, heparin lithium, EDTA potassium salt, have been exported to overseas, many customers long-term buyback, praise constantly. If you have vascular reagent needs, welcome to click on the website consultation!

Why does the serum separating gel produce bubbles?       Nowadays, with the improvement and development of the medical industry, many hospitals or institutions do not only focus on quality, but also have high requirements for beauty. If there are bubbles in a perfect transparent gel, it will not only damage the external image, but also the purchaser will question whether the transparent gel has a quality problem? This will affect the purchase volume. So today we will carefully analyze the bubble problem of serum separating gel.      

As an in vitro blood anticoagulant, EDTA-3K is widely used in vacuum test tubes. With the promotion of blood routine projects, the demand for EDTA-3K will only increase, and the sales of EDTA-3K in the market are also very optimistic.At this time, the number of manufacturers producing EDTA-3K also involuntarily increased, such as Hubei New Desheng, Shandong Suihua, Jiangsu Haolong, Hubei Chengfeng, etc., among so many manufacturers, is there any one with excellent production quality?   The answer is definitely yes. Here I would like to recommend a manufacturer of ethylenediaminetetraacetic acid tripotassium salt with better quality in China - Hubei New Desheng. Its EDTA-3K has stable performance and good anticoagulant effect. It is quite popular in the domestic market. Why do you say that? Let me introduce it to you in detail. 1. We produce EDTA-3K with high purity As an in vitro anticoagulant, EDTA-3K not only has high requirements on key values, such as solubility, pH value, appearance, etc., but also must ensure a purity of over 99%, and Desheng has achieved this. The purity is even as high as 100.5%, mainly because Desheng produces dihydrate EDTA-3K, which has a more stable moisture absorption capacity than anhydrous EDTA-3K.   2. Desheng is a Chinese manufacturer Although there are many merchants selling EDTA-3K, few are genuine manufacturers. Many people will encounter middlemen. Not only the price is high, but the product quality has also become a headache. And Desheng is a real Chinese manufacturer that can be used for on-site inspections. Once there is a problem with the goods, it is guaranteed to be returned without worry, and the price is always given to the customer with the manufacturer's discount, and there is no situation of making a difference.   3. Guaranteed delivery time and sufficient goods As a wholesale manufacturer, it has a large warehouse and advanced production equipment, and its production capacity can basically reach the ton level or even higher. There is also stock in the warehouse, which guarantees delivery on the same day or the next day, and can fully satisfy customers with large orders. Especially for foreign customers, Desheng will also keep separate inventory, although the distance is far away, it is guaranteed that customers will not be out of stock.   Desheng has always believed in "quality first", so since its establishment in 2005, it has been concentrating on various products to ensure that the products are of high quality and can stand the test of the market.We’ve fully established a good cooperative relationship with customers by its own brand.If you also have the demand for EDTA-3K or other blood collection tube additives, you may wish to use Desheng as a key partener, and we won’t fail your trust!