China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic and biochemical tests. The principle is that the tested substances react with enzyme,produce hydrogen peroxide (H2O2) which synthesized red Quinoneimine compound with the help of 4-aminotipyrine (4-AAP) and peroxidase (POD).Initially, phenol was used as a chromogen agent, and later, there were many kinds of compounds to replace phenol, which greatly improved the sensitivity of the reaction. There are many principles of in vitro diagnostic reagents or kits. For example: Enzyme-Linked ImmunoSorbent Assay (ELISA), enzymatic method, colloidal gold method, western blotting method, etc. Each methods can be applied to the detection of multiple substances. For example, based on the principle of Trinder reaction (also called enzymatic method), it can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglyceride, etc. There are several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder's reagents are stable enough to be used in both solution and experimental pipeline detection systems. With the help of hydrogen peroxide and peroxidase in oxidative coupling reaction, the new Trinder's reagent reacts with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfonehydrazone (MBTH),and forms very stable purple or blue dyes. The molar absorbance of the dye coupled with MBTH was 1.5-2 times higher than that coupled with 4-AA.However, the solution of 4-AA was more stable than the MBTH.The substrate is oxidized by oxidase and produce hydrogen peroxide. The hydrogen peroxide concentration corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled by the new Trinder's reagent and 4-AA. There are 10 new Trinder's reagents available in Desheng. TOOS is the most commonly used among them. However, for a specific substrate, testing different types of Trinder's reagents is necessary to develop the optimal detection system.

Compared with traditional techniques such as radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA) is a new technology based on chemiluminescence (CL) reaction, which has attracted much attention in the past decade.Due to the associated wide dynamic range and high sensitivity, this technique quickly become the predominant method for clinical immunological analysis. It is widely used in tumor, infectious disease, metabolic disease and pregnancy detection. There are three kinds of chemiluminescence immunoassay (CLIA). First one is chemiluminescence immunoassay (CLIA) Commonly used labeling substances are isoluminol, acridine esters, these labels can generate special luminescent groups and directly participate in the reaction during the analysis process. Directly labeled chemiluminescence immunoassay with acridinium ester: After taking immune reaction with the corresponding antigen (antibody) in the sample to be tested, a solid-phase coated antibody-antigen acridinium ester-labeled antibody complex substance. The oxidant (H2O2) and NaOH make the solvent to be alkaline, and the acridine ester decomposes and glow without catalyst.Substances like luminol for Chemiluminescence immunoassay: The luminescence of these substances is oxidation reaction. In alkaline solution, luminol can be oxidized by many oxidants, among which hydrogen peroxide is the most commonly used. Due to the slow reaction of luminescence, some enzymes or inorganic catalysts need to be added. Enzymes are mainly horseradish peroxidase (HRP), inorganic types include ozone, halogen,Fe₃+,Cu₂+,Co₂ and their complexes. Second is indirect luminescence immunoassay Commonly used markers are horseradish peroxidase (HRP, the common substrate is luminol or its derivatives, such as isoluminol) and alkaline phosphatase (ALP, the common substrate is 1,2-dioxane Ethane derivatives, AMPPD), such markers act as catalysts or energy transfer receptors and participate for luminescence reactions. Third one is electrochemiluminescence immunoassay technology A commonly used marker is ruthenium terpyridine with tripropylamine as a commonly used substrate. The three luminescence technologies have their own merits and are widely used in the diagnosis of different diseases. Chemiluminescence immunoassay technology is of great significance in the field of clinical diagnosis, and chemiluminescence reagents play a huge role. The isoluminol and acridine ester series products produced by Desheng Biochemical have stable quality and are supported by excellent scientific researchers and after-sales teams. 

Viral Transportation Medium for Covid-19 detection Nucleic acid testing is the standard for diagnosing Covid-19, and it is also an effective measure to accurately prevent and control the pneumonia. The mixed detection work in various places to further improve testing capacity and efficiency. At present, the laboratory adopts "10-in-1 mixed detection" technology for the Covid-19. But there are still a lot of people who don't understand the meaning of single and mixed detection. I will introduce them in detail next.   What is the meaning of single and mixed detection? Single detection: As the name suggests, one person's collection swab is placed in a collection tube at one time. Mixed detection: The collection swabs of 5 or 10 individuals are put into a collection tube at same time.When the test result is negative, all the mixed samples are considered negative, which means that the 10 people in the mixed test are all safe. If it is positive, the relevant departments will immediately isolate the 10 subjects in the mixed collection tube separately, and re-collect a single-tube swab for review, and then determine the positive one.   Which VTM (virus transportation Medium) should be selected for mixed collection? The virus transportation Medium is mainly used for the preservation and transfer of upper respiratory tract virus nucleic acid samples such as nasal swabs and throat swabs. In 2020, with the orderly work of fighting against the COVID-19 nationwide,the epidemic has been effectively controlled. In order to further improve the capacity and efficiency of testing,and effectively meet the needs of normalized epidemic prevention and control, the website of the National Health Commission issued the "Notice on Printing and Distributing the Technical Specifications for 10-in-1 Mixed Detection of COVID-19" on Aug 19. In order to prevent secondary infection, it is stipulated that 6ml of inactivated preservation solution should be used for mixed sampling, while single sampling only requires 3ml.   How accurate is the mix detection? The size of the single collection tube and the mixed collection tube are not the same, and the volume of virus preservation solution inside is also different. Based on the results of a large number of basic experimental researches in the early stage and confirmation of practical operations, the increase in the volume of the mixed preservation solution has no effect on the detection results of weakly positive specimens, so the accuracy is not a problem.   Under what circumstances are single and mixed detection used? Single detection: First, it is applied to key areas (confirmed cases and asymptomatic infections in communities with recent outbreaks, old communities, densely populated communities, floating population gathering areas, and people who have recently left the province), and others not suitable for mixed testing.Second,patients in medical institutions are used with single testing. Mixed detection: It is used for large population samples testing and the overall positive rate of the group is less than 0.1%.   As a professional manufacturer for virus transportation Medium, Desheng can provide two kinds of preservation solutions, inactivated and non-inactivated. Of course, whether it is a single or mixed collection tube, we can recommend the appropriate quantity according to the amount of detection people. Competitive price offered, welcome to inquire.http://www.whdsbio.cn/product/13.html

How To Get Refined Heparin?     For many people, heparin is a medicine. In fact, in addition to medicinal purposes, heparin has many other usages, such as made into additives for in vitro diagnosis,biochemical testing, blood collection and storage, or used as raw material of cosmetics. These heparins are purified and processed to refined heparin before using.   Sourcing of Heparin     The name of heparin is called because it was originally found in the liver. It is a natural anticoagulant substance exist in many organs of mammals, with the highest content in the lung and intestinal mucosa. Heparin is a kind of aminodextran sulfate extracted and refined from pig intestinal mucosa or bovine lung. It is a mixture with a molecular weight range of 3000-30000KD and an average of about 15000KD. Unfractionated heparin fission into aminodextran sulfate fragment,witch are low molecular weight heparin calcium enoxaparin and dalteparin with average molecular weight range of 3000-8000KD.   Prepare Crude Heparin     After fresh pig intestines (or after the natural thawing of frozen pig intestines) are carefully washed with water, enzymolysis is carried out: first,the raw materials are carefully adjusted to the pH value of 8-9 with a small amount of dilute lye under full stirring. Second,enzymatic hydrolysis at 37-40 degrees for 3-4 hours. Third,add about 5% of the total weight of the liquid (containing NaCL 95% min, calcium magnesium calcium salt 0.5% max) to mix evenly, then warming up to 90 degrees and keep for 30 minutes, finely filter. After adjusting the filtrate’s PH around 9.0-9.5, ion exchange adsorption treatment is carried out. Forth,elution, suction filtration, combined supernatant and filtrate, then adjusting PH range to 6.0-6.5, added 1.5 times the amount of 95% ethanol to precipitate overnight. the next day, carefully dried and sucked out the supernatant, collected the lower sediment, suction filtered to dryness (The original liquor can be used in the eluent before adjusting the pH value). After vacuum drying, rough heparin is obtained.   Configure Refined Heparin     Completely dissolving the crude heparin in 2% sodium chloride solution to make a solvent with a solubility of about 8%. The temperature can be appropriately raised to help dissolve during this process. Adjusting the PH of above solvent to 8.0-8.2 with 5mol/L sodium hydroxide solution, and raising temperature to 78-80 degrees, then adding 0.15-0.2mol/L potassium permanganate solution for oxidation. Finely filtrated by sterilizing filter, precipitated with 3-4 times of 95% ethanol.Last but not least, dehydrate with acetone, grind to fine, and dry in far-infrared vacuum (50-60 degrees) to obtain fine heparin sodium.         It can be seen from the above that the fined heparin sodium can be obtained from crude heparin after further refining.Hubei New Desheng Material Technology Co.,ltd is specialized in heparin used for blood tube collection, enoxaparin and cosmetic.For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html  

What Should Be Noticed When Titrate Disodium EDTA Standard Solution?   Disodium EDTA dihydrat is used as an anticoagulant in blood collection tubes. It is a white powder needs to be configured into a solution before used. The standard solution needs to be titrated. Two indicators should be used for calibration, because in complexometric titration, the acidity range of EDTA with different metal ions to form different stable complexes. Eliminate base effects to reduce errors.   When configuring disodium EDTA titration,the following points need to be paid attention: 1. Phenolphthalein cannot be used to replace methyl red when neutralizing HCL in the standard. Ammonia neutralizes hydrochloric acid, the product NH4cl is a strong acid and weak base salt,the PH is acidity, while phenolphthalein is an alkaline indicator, so it cannot be used as an indicator,and the discoloration range of methyl red is 4.4-6.2, so it can be used as an indicator. 2. Mg2+-EDTA can improve endpoint acuity Mordant Black can form a stable complex with Mg2+, and the hromogenic is very sensitive, but the complex formed with Ca2+ is unstable and has low color sensitivity. Therefore, when Ca2+ is titrated with EDRA in a pH=10 solution, a small amount of MgY is first added to the solution to make displacement reaction to replace Mg2+. 3. The titration must be carried out in a buffer solution In the process of complexometric titration,H+ is continuously released with the formation of complexes, so the acidity of the solution continues to increase.And reducing the stability of the complexes and destroys the optimum acidity range of the indicator discoloration, resulting in a large error. Therefore, in complexometric titration, it is necessary to add a buffer solution to control the PH of the solvent.   Hubei New Desheng Material Co.,ltd is a professional manufacturer of EDTA, witch quality is stable and reliable.For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

The responsibility of purchasing is to be able to buy good enough products at an effective cost. It is not so easy to buy high-quality TRIS base in the chemical industry. The news for TRIS in stock on the Internet is overwhelming, but the truth is the seller with small batches of reagents, or it’s out of stock and need to wait with weeks, and even worse is the price and delivery time can not be sure. How to find the real manufacturer of TRIS in the market? Procurement can make effort into search. Searching on google with some keywords such as "TRIS factory",or "TRIS manufacturer". Direct manufacturers can be quickly sorted out. Of course, there are also many traders with such words, which requires deep selection. In addition,try to collect some chemical platforms. Although there are many advertise for promotion,the products with TRIS are relatively concentrated. Generally, buyers in the chemical industry have several fixed platforms for inquiries. It will be more easier if the platform with selection of product supplies. However, there are still many manufacturers who only sell on part of the platforms or even without any promotion. They just rely on their frequent clients, that leads to poor information flow. In that way, it is a little bit difficult for buyers to find these factories with high-quality trimethylol Aminomethane.Desheng Biochemical is also a manufacturer of TRIS, and has taken many detours in the process of promotion. Using right keywords and industry websites, basically all the manufacturers of TRIS can be found. If Linkedin included,then more choices for purchasing.There are many kinds of buffers,and it is also a good method to add some information about the manufacturer of buffers.Companies with R&D capabilities and production capabilities can solve more problems for you to a certain extent!

The full name of TOOS reagent in Chinese is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, which is a chromogenic substrate reagent. Chromogenic substrates, also known as new Trinder's reagents witch are relatively common reagents in clinical biochemical testing projects, and TOOS is one of the most widely used chromogenic substrates. TOOS can be used in blood glucose monitoring, routine liver function detection, triglyceride and other blood lipid detection projects. The TOOS developed and produced by Desheng is a white powdery substance, which has obvious advantages compared with other manufacturers in terms of appearance and performance.Our TOOS has higher molar absorbance and more sensitivity than conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity, and the color of product is more stable.It not only shows good stability in some small measurement,the shape and color of the crystal powder are also purer, and the purity has reached 99%. Due to the special properties of TOOS, the prepared solution should be used at once. The solution will be oxidized and discolored when it exposed the air for a long time.This powdery substance is also more convenient to store, and its high solubility in water makes it easy to use even if it is configure before using. As a original manufacturer of TOOS, Desheng has been improving in terms of quality, price and service, adhering to the business philosophy of customer first. We are constantly making our services and products better for getting more affirmation from clients. As the saying goes,as long as you try very hard, your effort is rewarded.For 20 years harder working,Desheng has also successively gained the recognition and affirmation of more than 400 customers at home and abroad. We will continue to forge ahead.

HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic buffer, CAS No. 7365-45-9, this buffer is generally used in biochemical experiments to adjust the PH of the reaction system, such as virus preservation solution or cell preservation solution. It has no toxic on cells,and maintain PH for a long time, It is always applied in cell culture medium and cosmetics. HEPES buffer is a non-ionic amphoteric buffer with good buffering capacity in the pH range of 7.2-7.4. Its major feature is to maintain a relatively stable PH value in culture medium or cell observation. Under these standards, the caps of cell culture flasks should be tightened tightly to avoid required carbonate escaping into the air. The way to prepare the HEPES buffer. Hepes buffer can be added directly into the prepared culture medium with required concentration, followed by filter sterilization.Add 2.38 grams of HEPES powders to every 1000ml of culture solution, adjust the PH to 7.2 with 1NNaOH after dissolving, filter and sterilize before applying, at this time, the concentration of HEPES is 10mmol/L. Adding 99ml cell culture medium solvent into 1ml stock solution,the concentration of HEPES is also 10mol/L.Below is the way to prepare HEPES stock solution with 1mol/L. First, dissolving 23.8g HEPES into 90ml double-distilled water;Second,adjusting the pH to 7.5-8.0 with 1N NaOH; Third,making up to 100ml with water titrant;Forth,filtering and sterilizing before packed into 2ml bottle;At last stored at 4℃ or -20℃. With high temperature resistant and melting point up to 200°C,HEPES powder won’t be degraded by autoclave. According to relevant literature, if the HEPES aqueous solution is exposed to ambient light for three hours, toxic hydrogen peroxide (H2O2) will be generated. Therefore,2 points need to be paid attention to,one is the HEPES aqueous solution must be kept away from light so as not to affect the experimental results. After being configured as a solvent, it should be stored at 4 °C and used in a short period of time for better results.Another is generally placed in a dry room and cannot be directly exposed to sunlight for a long time. Hubei New Desheng Material Technology Co.,Ltd is specialized in producing biological buffers over years.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises. For more information, pls contact visit our website.

Disodium (EDTA-Na2), dipotassium (EDTA-K2) and tripotassium (EDTA-K3) are three kinds of Ethylenediaminetetraacetic acid (EDTA) salt，which commonly used as anticoagulant for testing. In order to ensure that blood does not coagulate, blood collection tubes containing anticoagulant are widely used in normal Routine blood test. EDTA dipotassium (EDTA-K2) is always the best choice with better anticoagulant effect,and little influence on the shape of blood cells. Because the solubility of potassium salt is better than EDTA-Na2,so EDTA-K2 and EDTA-K3 are more widely used than EDTA-Na2.Compared to EDTA-K3,EDTA-K2 can compensate for cell wrinkles caused by osmotic pressure with lower PH. EDTA-K2 pollution often causes abnormally high concentration of Potassium in patients, which is inconsistent with the clinical manifestations of patients.   However, EDTA-K2 pollution may also affect other biochemical indicators, but how much EDTA-K2 concentration effects, and what kind of conventional biochemical indicators affected ? This article by the lippi.g team in Biochem Med (Zagreb) systematically explores the effect of increased concentrations of EDTA-K2 on the results of routine biochemical assays.   Materials and methods: This paper recruited 15 volunteers from the laboratory staffs. Two vials of lithium heparin and one vial of EDTAK2 samples were collected from each study subject. EDTA blood sample was diluted with lithium heparin blood into different lithium heparin concentrations of 0%, 5%, 13%, 29% and 43% EDTA.Then,separating samples and testing biochemical indicators including ALT,bilirubin,cholesterol,creatinine,iron,LDH,lipase, potassium,sodium,chloride,magnesium and phosphorus.   Results indicate as EDTA2K concentration (from 5% concentration) increased, the amount of calcium, cholesterol, iron, lactate dehydrogenase, magnesium decreased,but potassium increased. The EDTA concentrations that significantly increased the results of the potassium were 13% and 29%. With the increase of the concentration of EDTA, the amount of ALT bilirubin and lipase did not change. Low concentrations of EDTA2K (from 5%) have greater effects on calcium, cholesterol, lactate dehydrogenase, magnesium and potassium. But for For sodium,phosphorus and iron,higher EDTA2K (from 29%) concentration needed.   Hubei Xindesheng Material Technology Co., Ltd. is a specialist of blood collection tube reagent manufacturers. The unit packaging volume of its anticoagulant EDTA dipotassium is 500g per bottle, and the shelf life is 3 years. It’s sold out to domestic and foreign customers. We have professional R&D team of blood collection tube anticoagulants with 19 years of experience.

Chromatography is often used for the separation and purification of proteins in laboratory.In this process, the PH variations is related to the separation capability of the system. If the PH of solvent is near to pKa, for example, small changes in PH can strongly affect retention, and consequently the separation results.A buffer should be added to the system in order to control the mobile phase PH, Since the retention of ionizable compounds is especially sensitive to the PH variable. According to relevant literature in chromatography,we found that although the Tris base and MES are often the best choices for this technique, other buffers have also been used in cation exchange chromatography, anion exchange chromatography, high-performance liquid chromatography (HPLC) and other similar techniques.Below are the 10 best buffers for your reference. 1) BIS-TRIS buffer Suitable PH range: 5.8 - 7.2 PKA (25°C): 6.46 Molecular weight: 209.2g/mol Used as a buffer during anion exchange chromatography (Concerns: several components in a chromatographic system may be competing for metal binding with this buffer) 2) BES buffer Suitable PH range: 6.4 - 7.8 PKA (25°C): 7.09 Molecular weight: 213.2g/mol Used as a binding buffer and eluent in cation exchange chromatography / as a buffer in gel filtration chromatography 3) Bicine buffer Suitable PH range: 7.6 - 9.0 PKA (25°C): 8.26 Molecular weight: 163.2 g/mol Used as a mobile phase buffer and eluent in cation exchange chromatography 4) CAPS buffer Suitable PH range: 9.7 - 11.1 PKA (25°C): 10.40 Molecular weight: 221.32g/mol Used as a binding buffer and eluent in cation exchange chromatography Read more about Hopax CAPS 5) HEPES buffer Suitable PH range: 6.8 - 8.2 PKA (25°C): 7.48 Molecular weight: 238.3g/mol Used as a binding buffer and eluent in cation exchange chromatography in a two-stage reverse dialysis method for in vitro release testing 6) MES buffer Suitable PH range: 5.5 - 6.7 PKA (25°C): 6.10 Molecular weight: 195.2g/mol Used as a buffer in capillary electrochromatography,gel-filtration chromatography,phosphocellulose column chromatography,hydrophobic interaction chromatography,and cation exchange chromatography 7) MOPS buffer Suitable PH range: 6.5 - 7.9 PKA (25°C): 7.14 Molecular weight: 209.3g/mol Used as a running buffer for protein purification in chromatography 8) PIPES buffer Suitable PH range: 6.1 - 7.5 PKA (25°C): 6.76 Molecular weight: 302.37g/mol Used as a buffer in cation exchange chromatography,it should be used in lower concentrations due to its large ionic strength and dependence of concentration on pKa.And a buffer in phosphocellulose chromatography to purify microtubule proteins 9) TAPS buffer Suitable PH range: 7.7 - 9.1 PKA (25°C): 8.40 Molecular weight: 243.28g/mol Used as a buffer in planar chromatography to separate dyes,and in ion exchange chromatography for enzyme purification14 10) Tris buffer Suitable PH range: 7.5 - 9.0 PKA (25°C): 8.06 Molecular weight: 121.14g/mol Used as a buffer and eluent in anion exchange chromatography,and in capillary electrochromatography due to its low ionic mobility Hubei New Desheng Material Technology Co.,Ltd is specialized in producing biological buffer over years.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises. For more information, pls contact visit our website.

About TRIS Buffer and TRIS-derived buffers     Buffer solution is usually a solution composed of weak acid and its conjugate base or weak base and its conjugate acid , which can change PH when a certain amount of other substances are added. The function of Buffer is to adjust the PH of the solution within a limited range so that the acidity of the solution to be tested conforms to the specified range by the analysis. In biochemical experiments and related research work, a large amount of buffer is required to maintain the PH of the testing system. Among them, TRIS and its derivative buffers are widely used in organic synthesis, coatings, fireproof materials, and biochemical and pharmaceutical fields. So, what are the advantages and disadvantages in practical applications? What is TRIS buffers?     Tris, also known as Tris (Hydroxymethyl) Aminomethane, Tris buffer is widely used, especially in nucleic acid and research experiments related to DNA, it has much more advantages than PBS phosphate buffer. Tris is also an important raw material for the synthesis of another biological buffer, TAPS.The advantages of Tris are very obvious. The PH range of tris buffer is 7-9, and the alkalinity is strong. Using this one buffer can configure PH buffers ranging from acid to alkali. At the same time, because it does not precipitate with metals,and with little interference with biochemical processes,tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many other important uses. With low ionic strength of Tris buffer ,it can be used for the formation of intermediate fibers of lamin in C. elegans.Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis.       There are disadvantages of TRIS buffer need to be noted:     First, the PH of TRIS buffer is greatly affected by concentration;     Second, the effect of TRIS buffer is changed by temperature,for example, at room temperature of 25°C, the PH of TRIS buffer is 7.8,but 8.4 when it’s reached at 4 °C and 7.4 at 37°C. Therefore, when the buffer is deployed at 4°C,but the test temperature is at 37°C, the results of its hydrogen ion concentration will be increase tenfold. At last, the buffering capacity is poor when the PH is lower than 7.5.   Several normal TRIS-derived buffers     TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservatives, etc.The PH of TBS buffer is 7.4. It is an isotonic buffered saline solution commonly used in biology, such as experiments in immunohistochemistry, situ hybridization, enzyme-linked immunosorbent assay. And washing non-specifically bound antibodies in western blot testing. TBS buffer is a salt buffer made by isotonic salt solution and TRIS-HCL buffer,mainly composed of TRIS-HCL, NaCl, tween20.The suitable PH range is 7.2-7.5.       TE buffer is a kind of electrophoresis buffer,prepared by TRIS and EDTA. The PH is 8.0. It is mainly used to dissolve nucleic acid, and store DNA and RNA stably.       TAE buffer is a buffer composed of Tris, acetic acid and EDTA. The PH range is 7.2-8.4. It’s a buffer widely used for short-term electrophoresis of large fragments of DNA.       TBE buffer is a solution composed of Tris base, boric acid and EDTA (ethylenediaminetetraacetic acid). TBE buffer is commonly applied in agarose gel electrophoresis to analyze DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning. Hubei Xindesheng Material Co.,ltd is a high-tech company specializing in the research and development, production and sales of biological buffers, blood collection tube additives, chemiluminescence reagents and luminescent substrates.       With seventeen years of rapid development, Desheng has a large R&D team and experimental base.We’ve developed a series of biological buffers including TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PIPES and so on. It’s not only have a large market share in the domestic market, but also have been sold to dozens of countries around the world. We’ve established long-term and stable cooperation with many large-scale biomedical technology enterprises.     For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html

3-(N-ethyl-m-toluidino)-2-hydroxypropane-sulfonic acid sodium salt (abbreviation is TOOS) is also named Sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate, which is a white powder with molecular formula of C12H19NO4S.Na and molecular weight is 331.36. TOOS is also called EHSPT, is often used in the following test kits: adenosine dehydrogenase detection kits for liver function testing, 5'-nucleotidase detection kits, glucose detection kits in blood glucose metabolism, glycation Albumin detection kit, 1,5-anhydroglucitol detection kit.TOOS with good characteristics water-solubility, high sensitivity and strong stability. The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic assays and biochemical tests. They have several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity.The new Trinder's reagents are stable enough to be used with both in solution and experimental pipeline detection systems. With hydrogen peroxide and peroxidase, the new Trinder's reagent react with 4-aminoantipyrine (4-AA) or 3-Methylbenzothiazolesulfonehydrazone,and form a very stable purple or blue dye.The molar absorbance of dye formed by new trinders’ reagent reacting with MBTH is 1.5-2 times higher than with 4-AA; But MBTH solution is more stable. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of this hydrogen peroxide corresponds to the concentration of the substrate. Therefore, the amount of the substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol , acyl-CoA, and cholesterol can be used to detect those substrates coupled to the Trinder's reagents and 4-AA.TOOS is the most commonly used among 10 novel trinder’s reagent.However, it is necessary to develop more kinds of trinder’s reagent to match the specific substrates.     The chromogenic reagents produced by Desheng are impeccable in terms of purity, sensitivity, stability and appearance. The strict control of raw materials by quality inspection department from storage to production,guarantee the quality of chromogenic reagent.Only an enterprise that focuses on product research and development can provide customers with assurance. pls contact visit our website.