China Wuhan Desheng Biochemical Technology Co., Ltd Company News

According to news reports, autumn and winter is the season of rapid spread of the new coronavirus, and the epidemic may continue to spread rapidly in this winter and next spring. In the special period of epidemic prevention and control, rapid and efficient detection, diagnosis and isolation of virus infected patients are the key to overcome the epidemic.   New Coronavirus pneumonia was detected by real-time fluorescence RT-PCR kit and virus gene sequencing in two ways. Among them, nucleic acid detection (fluorescent PCR) has become the main means of new coronavirus detection because it can get the detection results faster, has greater flexibility and universality, and is more suitable for epidemic prevention and control and management.     The principle of New Coronavirus nucleic acid detection kit is basically: by extracting RNA from patient samples, and performing reverse transcription polymerase chain reaction (RT-PCR), amplifying the virus information in the sample to amplify it, and finally reading the signal in fluorescence. If the signal is positive after PCR, it can be said that there is virus in the sample (already infected), otherwise it means that there is no infection.   Affected by the epidemic, the rapid increase in the production capacity of nucleic acid detection kits has also brought a sharp increase in the demand for reagent raw materials. Overnight, the virus preservation solution has become a hot product. According to the published reports, the reagents used in the coronavirus nucleic acid detection kit include Tris, HEPES, Tris HC, guanidine hydrochloride, BSA, guanidine isothiocyanate, EDTA, edta-2na, etc.   Among them, Tris, Tris HC and HEPES are similar in their functions as buffers, which are used to form PCR reaction solution / RT-PCR reaction solution / thermostatic amplification buffer, etc.; BSA is mostly used as a stabilizer in the preservation solution and reaction solution of restriction enzyme or modified enzyme;   Guanidine hydrochloride and guanidine isothiocyanate are commonly used reagents for nucleic acid extraction. Guanidine hydrochloride is a strong inhibitor of nuclease, which is conducive to extracting complete RNA from RNase rich tissues. Guanidine isothiocyanate is a powerful protein denaturant, which can quickly separate nuclear protein from nucleic acid; EDTA and edta-2na are mainly used as chelating agents to prevent metal ions such as Mg and Ca from activating protease. In order to prevent the rapid degradation of RNA, ensure the integrity of RNA and improve the accuracy of detection, virus preservation solution plays an important role.   The novel coronavirus pneumonia and the virus preservation solution play an important role in the diagnosis of diseases. In the very period of epidemic prevention and control, it is necessary to diagnose the new crown pneumonia.   Desheng is a new scientific and technological enterprise with "in vitro diagnostic reagent raw materials", "blood vessel additive", "biological buffer" and "pharmaceutical intermediates" as the leading, integrating R & D, production, sales and service.   At present, Desheng can provide nearly 100 kinds of in vitro diagnostic reagent materials, including Tris, Tris HC, HEPES, EDTA, acridine ester and other reagents, as well as virus preservation solution, the core material for detection. The average daily production of Tris can reach 1 ton, hoping to provide basic raw materials for the development of new crown vaccine, neutralizing antibody and rapid immunodiagnostic reagent.

Desheng, as an old manufacturer of vascular additive, has developed from a small workshop to a large factory. In the world of ups and downs, it can keep its progress all the time, which only means that it has been keeping up with the pace of the times, striving to do well in every product, so that all the individuals and enterprises who have contacted their products applaud. I think the success of an enterprise in the final analysis is inseparable from the quality of products and services.   Since it is an old manufacturer of vascular additive, what are their vascular additive products? Serum gel, heparin sodium, heparin lithium, dipotassium EDTA, Tripotassium EDTA, coagulant, silicide, sodium citrate, potassium oxalate, etc. Every product has its own characteristics and uses, but who is one of them? From the use, sales and production point of view, serum separation gel is worthy of the title.     Serum gel is a substance used to separate serum (plasma) and blood cells (blood cells). The separation gel can be used not only with accelerator, but also with anticoagulants such as heparin salt, EDTA salt and sodium citrate. The purpose of using separation glue is to prepare high-quality specimens and to store and transport specimens.   If separation gel is not used in coagulant blood collection vessel, although it can also accelerate blood coagulation and form blood clot and serum separation, there will still be material exchange when blood clot contacts with serum for a long time, and blood cells are very fragile and easy to hemolysis and interfere with serum samples. Using separation gel separation layer can solve the problem. In general, 0.8-1.2g separation glue is added to each blood collection vessel to form a separation layer of at least 5mm between different blood components, so as to ensure the high quality of blood samples.   The pure EDTA additive is the blood routine tube, while the EDTA dipotassium and serum gel are used together for the collection, transportation and storage of venous blood samples for nucleic acid detection. It is aimed at the DNA amplification detection of HBV, HCV and HIV. The serum gel can well block the interference of hemoglobin in red blood cells to the nucleic acid detection experiment.   We invest a lot of manpower and material resources in the serum separation gel every year. Last year, we obtained a patent due to the improvement of the separation gel technology, which increased our output from 500 tons to 800 tons per year in the early stage. The sales volume is also greatly increased, and it is sold at home and abroad. No matter from which aspect, serum separation gel is one of the blood vessel additives. And our other products are only relative to the separation glue, may not be so brilliant, but the market share is also praiseworthy.

Carbomer wins the hearts of many people because of its powerful application functions. However, in the process of using, there are always problems like this and occasionally irritate you and make you crazy. If you encounter the following problems, that's great. I hope these can help you solve the problems you have encountered and liberate your hair.   Solubility issues Due to the special structure of carbomer, it is easy to swell in contact with water, and it has strong hydrophilicity. The carbomer of dry powder is very hygroscopic. Like other hygroscopic powders, it should be treated improperly. When thrown into water or other polar solvents, it tends to form agglomerates or is not completely wetted. Other powders will eventually decrease and dissolve after forming agglomerates, but the carbomer will not dissolve easily after forming agglomerates, because once the outer layer of the agglomerates is completely infiltrated, the water will not easily penetrate into the internal dry part.   Gel viscosity problem Temperature has a certain effect on carbomer gel. As the temperature rises, the kinetic energy of molecular motion increases, and the speed of motion increases. Due to the difference in the volume of gel molecules and water molecules, the movement frequency of the two is inconsistent. As the temperature increases, the hydrogen bond between the gel molecules and the water molecules weakens, and some of the hydrogen bonds are broken, which leads to the system’s failure. The viscosity is reduced. However, this effect is reversible, heating within 100 degrees and then returning to room temperature will restore the viscosity; if heated to 260 degrees, it will decompose completely in half an hour.   Color problem There are residual polymerization inhibitors, the type and amount of initiator, and the drying temperature. If the drying temperature is too high, the product is easy to change color. Studies have found that if the drying temperature exceeds 120°C, the product is more or less slightly yellowish. Therefore, drying should be dried under reduced pressure below 80°C.   Transparency issues In some gel products, such as disinfectant and disposable gels, ethanol is often added as an additive in order to increase permeability, corrosion protection, and solubilization, and the content may be as high as about 75%. Carbomer gel is essentially a polymer solution. The reason why the polymer solution is stable is that there is a hydration film on the surface of the particle. The addition of a strong hydrophilic non-electrolyte (such as ethanol) will cause the polymer solution to flocculate. Carbomer ethanol gel is prepared by different operation processes, and the ethanol concentration of the finished product is different. When the ethanol concentration is too high, the finished gel will produce a little opalescence, which reduces the transparency of the gel.   Stability issues When carbomer dissolves in water to prepare a gel, it is best to soak it in deionized water for 24 hours, and then mechanically stir it for half an hour. Neutralizing carbomer usually uses triethanolamine and EDTA-2Na as gel stabilizers. The presence of the hydration film on the surface of the carbomer gel makes the gel relatively stable. The greater the degree of dissociation of the carboxyl group, the less free water content in the gel, and the less prone to bacteria growth. The degree of hydration of the gel can be judged by the sliding time of the gel on the wall of the beaker. Products with the same viscosity but different hydration speeds indicate that the stability of the gel is different. The stability of the gel can be determined by the degree of hydration. Judge, adjust and control.

With the outbreak of the epidemic, Carbomer raw material has always been an important raw material in short supply. It is mainly used in daily chemical products. Because of the need of epidemic prevention, hand sanitizer is widely used, and carbomer is an important raw material of the product. Therefore, the shortage of carbomer once led to the shortage of hand sanitizers. At that time, cabom went crazy overnight. There are people everywhere asking about cabom. It is conceivable that the price has soared all the way, but it is not satisfactory. There is an unprecedented phenomenon that there is no goods at all. This also led to the behavior of some unscrupulous businessmen shoddy. The quality of carbomer's products was once suspected, and the price difference of middlemen was raised, which caused great losses to those enterprises that really needed carbomer's raw materials.   In this severe situation, it is very difficult to find a reliable and quality guaranteed manufacturer of carbomer. Because people's habitual thinking always feel that the wolf is not worth believing, even if it is true to meet you, there will be all kinds of distrust and suspicion. Just in this case, we met it -- Desheng technology, an enterprise that depends on products. It doesn't talk nonsense with you. It sends samples directly for testing. If it's true or not, you can tell by a try. No matter how much exquisite language there is no real product effect to experience, people are convinced.     Carbomer 940 of Desheng [character] white loose powder; characteristic slight odor; hygroscopicity. [identification] take this product 0.1g, dispersed in 20ml water, add 10% hydrogen Sodium Chloride Solution 0.4ml, that is gel form. [inspection] Acidity: take 0.10g of this product, evenly disperse and swell in 10ml water, and check according to law, the pH value should be 2.5-3.5.   Viscosity: take 0.5g of this product, evenly disperse it in 98ml water, after full swelling, mix it well, adjust the pH value to 7.3-7.8 with 15% sodium hydroxide solution (test with precision pH test paper), add water to 100ml, mix it well (avoid bubbles), and determine according to law, the dynamic viscosity at 25 ℃ should be 15-30pa · s.   Benzene: take about 1g of this product, weigh it accurately, put it in a tube with plug, add 10.0ml p-xylene, shake it to disperse, add 10.0ml 0.1mol/l sodium hydroxide solution, shake it tightly for 1 hour, and take the supernatant as the test solution. Take an appropriate amount of benzene, weigh it precisely, add p-xylene to make a solution of 10 μ g per 1ml as the reference solution. 1 μ l of the test solution and 1 μ l of the control solution were measured by gas chromatography (Appendix V E). A 3M glass chromatographic column with 201 red support (60-80 mesh) was used. Dinonyl phthalate and organic bentonite were mixed as the fixed solution. The coating concentration was 10%. The column temperature was 100 ℃. The benzene content should not exceed 0.01%.   Weight loss on drying: take this product and dry it under reduced pressure at 80 ℃ for 1 hour. The weight loss shall not exceed 2.0%. Burning residue: take 1.0g of this product and check it according to the law. The residue shall not exceed 2.0%. Heavy metal: the residue left under the item of burning residue shall be inspected according to law, and the content of heavy metal shall not exceed 20 parts per million.   [content determination] take about 0.4g of this product, accurately weigh it, evenly disperse it in 400ml water, stir to dissolve it, titrate it with sodium hydroxide titrant (0.25mol/l) according to potentiometric titration (Appendix VII a) (at the end point, stir at least 2 minutes after each drop). Every 1 ml sodium hydroxide titrant (0.25 mol / L) is equivalent to 11.25 Mg - COOH.   In the production process, De Sheng has passed through the numerous checkpoints and tests, and now has the quality of carbomer 940. The powder is white and flawless, and the gel liquid is crystal clear.

Acridine ester DMAE-NHS is a luminescent chemical reagent with a yellow solid powder appearance. Because no catalyst is needed, the reaction conditions are mild, and the reproducibility is good, the application field is very wide. Acridinium Ester-DMAE-NHS is mainly used for: chemiluminescence and immunoassay, receptor analysis, nucleic acid and peptide detection, etc. It also plays an important role in the TSH screening test items and can also detect the thyroid. Understand its four main characteristics.   Luminescence properties of acridinium ester-DMAE-NHS The luminescence of acridinium ester-DMAE-NHS is flash type, the luminous intensity reaches the maximum at 0.4s, the luminous intensity decreases rapidly in the first 2s, and the luminous intensity decreases slowly after that. The luminous half life is about 0.9s. Changing the concentration of acridinium ester-DMAE-NHS only affects the size of the luminous intensity, but does not affect the time and half-life of the maximum luminous intensity. Thermal stability of acridinium ester-DMAE-NHS The thermal stability of acridinium ester-DMAE-NHS decreases with increasing pH and temperature. At room temperature, DMAE-NHS is relatively stable in PB buffer with pH of 5.8, 7.0, and 8.0. After 16 days, the luminescence activity decreased by 1.6%, 3.6%, and 8.3%, respectively. At 37℃, the luminescence activity of DMAE-NHS in PB buffer with pH 5.8, 7.0 and 8.0 decreased by 8.9%, 22% and 42% respectively after 16 days.   Hydrolysis rate of acridinium ester-DMAE-NHS The reason why the luminescence activity of DMAE-NHS in PB buffer decreases with time is due to hydrolysis, which is beneficial to hydrolysis in an alkaline environment. Increasing temperature is also beneficial to hydrolysis, that is, the hydrolysis rate of DMAE-NHS varies with the pH of the solution. Increase and speed up with temperature rise.   Advantages of Desheng Acridine Ester DMAE-NHS Compared with traditional acridine esters, DMAE-NHS has higher luminous efficiency, better thermal stability, and stronger hydrophilicity. The chemiluminescence reagent has high photon yield and low background. It is compatible with proteins, antigens, and antibodies. After coupling, the luminous efficiency is almost unaffected, making it an excellent chemiluminescence immunoassay labeling reagent.   Desheng acridine ester DMAE-NHS is a golden yellow powder under normal conditions. The texture is very fine and bulky. The purity of the HPLC method is greater than 98%, no peculiar smell, high sensitivity, high luminous efficiency, and strong stability. .

Pharmaceutical intermediates refer to chemical raw materials or chemical products used in the process of pharmaceutical synthesis. From the development history of China's pharmaceutical intermediates industry, after nearly 30 years of development, pharmaceutical intermediates have developed from a small branch of the chemical industry to a new industry with an output value of billions of yuan, and its market competition is becoming increasingly fierce. Now Desheng Xiaobian will introduce several common pharmaceutical intermediates for you.   Tris (trimethylol aminomethane) Tris actually has another name, tromethamine, which is also related to its role in the field of medicine. In the field of medicine, Tris is also widely used in medicine. Tris is the intermediate of fosfomycin tromethamine. Fosfomycin tromethamine is a kind of broad-spectrum antibacterial drug which was launched in the late 1980s. Its bioavailability is 3 ~ 6 times of that of fosfomycin calcium, which is more suitable for the treatment of urinary tract infection and other diseases. Tris, as an organic amine, can form water-soluble double salt with insoluble substances with acidic groups. The most common eye drops containing Tris are ketorolac tromethamine eye drops.     Methyl 4,4 '- dimethoxyacetoacetate Methyl 4,4 '- dimethoxyacetoacetate is an important intermediate of nifedipine for the treatment of cardiovascular and cerebrovascular diseases. It is the leading drug for the treatment of cardiovascular and cerebrovascular diseases in the international market, but it has not been produced in China. Methyl 4,4 '- dimethoxyacetoacetate was synthesized from glyoxylic acid and trimethyl orthoformate in the presence of concentrated sulfuric acid. The latter reacted with methyl acetate and sodium methoxide to obtain methyl 4,4' - dimethoxyacetoacetate.   Pazopanib Pazopanib is a new oral angiogenesis inhibitor developed by GlaxoSmithKline company, which can interfere with the angiogenesis required for the survival and growth of refractory tumors. It targets vascular endothelial growth factor receptor (VEGFR) and works by inhibiting the angiogenesis of tumor blood supply. It is suitable for the treatment of advanced renal cell carcinoma (a type of renal carcinoma with cancer cells found in renal tubules), soft tissue sarcoma (STS), epithelial ovarian cancer and non-small cell lung cancer (NSCLC).   To sum up, Tris can be used as pharmaceutical intermediates. In addition to pharmaceutical applications, it can also be widely used in in vitro diagnosis, biopharmaceutical, cosmetics, paint and other industries. Desheng is a professional Tris reagent manufacturer, and its customers are the most in the application of buffers. Due to its high buffering efficiency and guaranteed quality, there are more than 50 stable cooperation customers at home and abroad .

Various exogenous and endogenous factors, such as environmental pollutants, ionizing radiation, drug chemotherapy, and cell metabolism, can cause various forms of DNA damage. Among them, DNA double-strand breaks have the most serious damage to genome stability and can threaten the survival of cells. Establishing a simple, rapid and sensitive method for detecting DNA hybridization and genotoxicity effects is a very meaningful research topic. Here is a brief introduction to the method of DNA damage detection.   What are the types of DNA damage detection DNA damage detection methods include gel electrophoresis, ultraviolet spectrophotometry, fluorescence and electrochemistry, and chemiluminescence analysis. Chemiluminescence analysis (CL) has been widely used in the fields of environment and life sciences due to its high sensitivity, wide linear range, convenient operation, fast analysis, and easy automation. Formaldehyde and acetaldehyde are both environmental pollutants. To a certain extent, it can cause DNA damage. Study the amount of acridinium esters embedded in DNA before and after the damage of formaldehyde and acetaldehyde, causing changes in the chemiluminescence intensity of acridinium esters, and study the damage behavior of formaldehyde and acetaldehyde on DNA. Establish a simple chemiluminescence analysis method to evaluate the degree of DNA damage caused by formaldehyde and acetaldehyde. Acridine esters method for detecting environmental damage to DNA 1. First, soak the glass luminescence cell used in a certain amount of concentrated nitric acid for several hours, acidify, and then rinse with water. Add 20 μL of 1mg/mL calf thymus DNA to the acidified luminescence cell, and place it for 1 hour to make The DNA is adsorbed on the bottom of the light-emitting pool, and then the unadsorbed calf thymus DNA is washed with secondary water to obtain the light-emitting pool with DNA adsorbed.   2. Add 50μL of 9.6×10-7g/mL acridinium ester to the luminescent cell, and the chimerization reaction is for 1h to embed the AE in the DNA double-stranded structure, and wash away the unchimeric AE with secondary water. Finally, in the luminescent cell Add luminescence initiation reagents in sequence to detect the chemiluminescence generated by AE chimerized in DNA. When detecting the damage of calf thymus DNA by formaldehyde and acetaldehyde, add damage reagent to the DNA after AE chimerization, and use it after a certain period of time. The second water washes away the AE released after the DNA is damaged, and the luminescence initiation reagent is added to detect the chemiluminescence intensity of the AE chimeric in the DNA.   Studies have shown that acridinium ester can be used as a chemiluminescence indicator with a good structure of intercalating DNA double helix. This detection method is feasible for DNA break damage and chemiluminescence detection method. It has the advantages of simplicity and sensitivity. Damage studies provide a simple research method. Desheng acridine ester luminescent markers have the properties of good hydrolytic stability, thermal stability and high signal-to-noise ratio, and the labeling conditions are mild, the labeling rate is high, and the separation does not affect the separation after labeling. , So it is considered to be an ideal chemical marker.

When it comes to acridine ester, many friends who want to buy it will have a headache, because it is too difficult to find a real manufacturer. Generally, they are dealers. Desheng biochemical is one of the few professional manufacturers of chemiluminescence reagents, especially acridine ester series. There are six different groups, different characteristics, and the characteristics of various groups are also different, which meet the needs of all kinds of testing.   Nsp-dmae-nhs belongs to acridine ester, acridine salt and acridine hydrazide. The characteristic of nsp-dmae-nhs is to increase the steric hindrance and enhance the hydrolysis resistance. Nsp-dmae-nhs is more suitable for some luminescent reagents that can not be changed to detect solvents.   After experimental comparison, nsp-dmae-nhs at room temperature, pH 7.0, luminous efficiency is also very slow to reduce, with NHS active ester, conventional coupling reaction. In the market, the sales of this model is also much higher than other models.     Although the luminous efficiency of acridine ester is much higher than other luminous reagents, many manufacturers still choose other luminous reagents. The reason is that the price of acridine ester is much higher than other reagents. Of course, if the buyer finds a dealer, then the price is more likely to double.   Cas194357-64-7, acridine ester nsp-dmae-nhs purity > 98%, yellow powder, Desheng biochemical was established in 2005, focusing on blood test reagent manufacturers, products now include many fields, but the more advantages have to mention acridine ester series, from research and development to detection to production, Desheng a professional research and development team to operate, laboratory production, not only batch difference Small size, low background and high luminous efficiency.   Acridine ester series has been used in major domestic enterprises, and exported to foreign countries to form stable sales, establish cooperative relations, good product quality, price concessions, hope to let more friends in need see contact us.

Acridine ester chemiluminescence reagent is a commonly used detection reagent in the field of in vitro diagnostics. Its chemiluminescence is not like the substrate of HRP or ALP and can directly chemiluminescence. So what is the difference between the chemiluminescence of acridinium ester and the fluorescence in fluorescence immunity? What's the difference?   Chemiluminescence is a molecular luminescence phenomenon in which the product returns from the excited state to the ground state when a substance produces a chemical reaction. In fact, there are three types of molecular luminescence: chemiluminescence, fluorescence, and phosphorescence. Their luminescence principles are different: Chemiluminescence in nature 1. Principles of Chemiluminescence For example, the chemical reaction between acridine ester and hydrogen peroxide produces another acridinium ester derivative. The energy released by the reaction is absorbed by the molecules of this product and transitions to an excited state, and the excited molecule returns to the ground state. Light radiation is generated during the process. The product here is a luminous body, and the luminescent product directly participates in the reaction during this process, so it is called direct chemiluminescence. Luminol's luminescence principle is similar, but requires HRP or POD catalysis, so it is called enzymatic chemiluminescence.   2. The principle of fluorescence When light irradiates certain atoms, the energy of the light makes some electrons around the nucleus transition from the original orbit to a higher energy orbit, that is, transition from the ground state to the first excited singlet state or the second excited singlet state. The first excited singlet state or the second excited singlet state is unstable, so it will return to the ground state. When the electron returns from the first excited singlet state to the ground state, energy will be released in the form of light, so fluorescence is generated.   3. The principle of phosphorescence When a certain room temperature substance is irradiated with incident light of a certain wavelength (usually ultraviolet or X-ray), it absorbs the light energy and enters an excited state (usually with a spin multiplicity different from the ground state), and then slowly de-excites and emits a ratio. Outgoing light with a long wavelength of incident light.   Seeing this, many people may still not be able to distinguish, but it doesn't matter. For example, the luminescence of fireflies belongs to bioluminescence or chemiluminescence, phosphorous fire or "ghost fire" is also chemiluminescence, and fluorescent sticks are also chemiluminescence; ultraviolet rays illuminate RMB anti-counterfeiting signs to emit fluorescence; luminous pens and luminous watches belong to phosphorescence. In daily life, people usually call all kinds of faint light fluorescence in a broad sense.   In the field of analysis and detection, chemiluminescence immunoassay and fluorescence immunoassay are two different detection methods, which need to be distinguished. Luminol and acridine esters of Desheng belong to chemiluminescence reagents, and the reagents for fluorescence immunity are reagents of different systems.

Desheng Material Technology Co., Ltd. carbomer spot sufficient, price reduction, volume promotion, manufacturers direct sales, spot direct supply, to avoid middleman price difference, buy carbomer discount in the end.   This article is just a brief introduction of carbomer. If you want to consult carbomer or want to know more details and specific price information, please contact us by telephone or go to Desheng website for detailed consultation.   The event is targeted at products, Carbomer 940 and carbomer 980, Carbomer is routinely used in hand free gel, daily chemicals, medicine and so on. In the use of more attention to viscosity and transparency and other issues, and Desheng material production of carbomer with pure white powder, high transparency, viscosity can be adjusted according to the direction of use and other characteristics.     Desheng was founded in 2005, specializing in polyacrylic acid material products. So far, it has developed three generations of serum separation gel with different uses and carbomer products for non use. Professional R & D team is one of the important conditions for Desheng to have confidence in the market, and market feedback is the best touchstone.   The big difference between Desheng carbomer and ordinary carbomer on the market is that the powder of Desheng carbomer is obviously white, and the powder is more fluffy and delicate. In the configuration of solvent, the transparency is also much higher than that of similar products. As Desheng is a direct manufacturer, individual indicators can also be customized.   No matter it is used by direct customers or purchased by traders, it is a very good choice. It can be purchased in small quantities, or it can sign annual contracts to enjoy ultra-low price discounts. Product quality is guaranteed at the same time, unlimited price concessions, after-sales service to enjoy product quality problems can apply for direct return, or refund, real manufacturers, reliable products, choice is very important!

In the nucleic acid detection of the new coronavirus, the very key technology is the real-time fluorescent quantitative PCR experiment of nucleic acid, which is the core technology of the new coronavirus detection. So what effect does the virus transport media used for virus sampling joints have on the PCR amplification of nucleic acids? This article makes a brief discussion. Does the virus transport media affect nucleic acid PCR amplification? Judging the result from the PCR amplification curve: The fluorescent quantitative PCR instrument in the new crown detection has become a routine equipment for molecular biology research. The rapid development of this detection method and the urgency of the detection task have also put forward higher requirements for the detection personnel, requiring them to be proficient in judging the results of the data. For the judgment of the result of fluorescence quantitative PCR, the most intuitive judgment is to see whether the amplification curve is normal. In normal experiments, you will encounter problems with abnormal amplification curves, such as discounted amplification curves, poor repeatability between multiple wells, and uplifted amplification curves of negative samples.   Reasons for abnormal PCR amplification curve: 1. Confirm whether the software settings are correct. Check the kit instructions to check whether the time, temperature, cycle number, fluorescence collection, etc. are set correctly, and whether the selected reagent contains ROX as a reference fluorescent dye. Some results show that the amplification curve of the multi-component graph is not uplifted, which is obviously a negative sample, but the amplification graph curve is uplifted, so that it crosses the threshold line, and instead has a CT value. This is because the software makes a mistake in the automatic deduction of the baseline. The method of manually adjusting the baseline can be normal by redefining the baseline.   2. Make sure that the consumables and instrument accessories are used correctly. Consumables are more important for real-time PCR. Many abnormal amplification curves are caused by improper use of consumables.   3. To determine whether the reagents used are normal, first determine whether the reagents are effective, including checking whether the reagents are within the validity period, whether they are repeatedly frozen and thawed many times, and whether the transportation conditions are normal. If all of the above are normal, then you have to consider whether there is any negligence in the operation details.   From the above points, it can be seen that the virus transport media will not directly affect the amplification curve, it will only affect the virus samples, but this can be done through a control experiment with positive samples to determine whether the virus preservation solution has an impact on the virus samples. . There are two types of Desheng virus transport media, both the inactivated type and the activated type are suitable for nucleic acid PCR experiments.

Nowadays, as long as people have a headache and brain fever, they first go to the hospital for testing to find out where the problem lies, and then prescribe the right medicine. In fact, many people only know the test items, but they don't know much about the test materials. The DAOS in the new Trinder’s reagent is a very important test material, which has the advantages of good water solubility, high sensitivity, and strong stability. Let's take a look at the past of DAOS on the road of detection. DAOS can be used for cholesterol testing When measuring total cholesterol, the cholesterol ester is firstly hydrolyzed into cholesterol and fatty acid by cholesterol esterase CHE, and then it is catalyzed and oxidized to 4-cholestenone and hydrogen peroxide by cholesterol oxidase, and then it is through DAOS and 4-AAP Coupling with hydrogen peroxide to produce a color reaction under the catalysis of POD, the concentration of total cholesterol can be determined by measuring the absorbance.   DAOS can be used for triglyceride detection When detecting triglycerides, triglycerides are hydrolyzed into glycerol and fatty acids in the presence of lipoprotein esterase (LPL); glycerol and ATP are catalyzed by glycerol kinase (GK) to generate glycerol-3-phosphate and ADP; glycerol-3- Phosphoric acid and oxygen are catalyzed by glycerol-3-phosphate oxidase (GPO) to generate dihydroxyacetone phosphate and hydrogen peroxide, and then use the color substrate to couple 4-AAP and hydrogen peroxide to produce color as in the above steps. In response, the TG concentration was measured.   DAOS can be used for high-density lipoprotein detection When detecting high-density lipoprotein, a dual-reagent reaction is used. Reagent one contains polyanion and surfactant, which selectively binds to VLDL and LDL, and inhibits COD and CEH in reagent two on VLDH-CH and LDH-CH. , Thereby selectively acting on HDL-CH, through the action of CEH-COD-POD, and then measuring the absorbance to determine the concentration of HDL, and finally the LDL concentration can be obtained by calculation.   Cautions when using DAOS: 1. Divided packaging: When taking a small amount of mg DAOS, the product needs to be divided into the quantity needed each time to reduce the number of bottle openings and prevent the product from absorbing moisture. 2. Use: When using the product, you need to take out the product from the freezer half an hour in advance and place it at room temperature. For the remaining, store the remaining DAOS in a sealed and light-proof container to avoid quality problems such as changes in color or properties of the product. 3. Preservation: Place DAOS in a 0-5 degree freezer, and record the time and batch number. 4. Transportation: Put ice cubes in the foam box for the packed products and wrap the products with foam glue. Take care to prevent the water from the ice cubes from getting the product wet (we put two more if the temperature is high, and the weather can be changed in winter. Temperature to decide)