China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The chemiluminescence labeling method represented by acridine ester and luminol is the preferred scheme for the quantitative detection of ligand conjugates. These assay markets have a high demand for technology, which requires detection technology to be highly sensitive, less susceptible to interference and easy to use. The chemiluminescence process has sufficient sensitivity, so it is very suitable for these applications. Old fashioned assays require the separation of bound markers from unbound markers. Although the assay is easier to develop, it is somewhat complex to implement. On the contrary, the chemiluminescence labeling method represented by acridine ester adopts homogeneous determination, which affects the luminescence reaction in some way through the combination of ligand and its receptor, which is easy to observe and the dose required for the reaction is small. Chemiluminescence is a series of radioisotope based analytical procedures. Its feasibility is proved to be applicable to various analytes, including high molecular weight and low molecular weight substances. The stability and analytical performance of reagents are of great significance to improve and maintain high-quality analytical performance. Most importantly, the development of ICMA technology based on labeled antibodies has the potential to increase the sensitivity of determination. This technique may prove valuable in testing and monitoring infectious agents and tumor markers. We have done experiments in which the natural luminol reaction product is incorporated into globulin, and the luminescence of its derivatives is 100 times that of diazonium salt. This also confirms the potential of Lumino. Chemiluminescence of luminol and acridine ester occurs under alkaline conditions, although in the case of luminol, there are catalyst requirements. The catalyst can be a simple transition metal cation, such as Mn + + or Ni, but it can also be complex macromolecules, such as horseradish peroxidase and cytochrome. The most popular catalyst is micro peroxidase because of this efficient reaction. Under relatively mild conditions. This requirement for catalysts makes the reaction vulnerable to the interference of substances in biological samples and powerful. Desheng bio produces chemiluminescent reagents, such as luminol, isoluminol, acridine ester, etc. In addition, it also independently develops and produces dipotassium and Tripotassium EDTA, with high purity, content of more than 99.5%, good anticoagulant effect and no impurities. After detection by SGS inspection organization, the content of trace elements is one order of magnitude lower than the national and industrial standards (10 times), with good clarity and high solubility. 80 grams of dipotassium and Tripotassium can be quickly dissolved per 100ml of water at 25 ℃, It can maintain the original properties of blood to the greatest extent and has no significant impact on the blood clinical test results. It has been favored and affirmed by well-known enterprises at home and abroad, including Yangpu!

Tris industrial grade, analytical grade, pharmaceutical grade, conventional packaging 25kg/barrel, white crystal powder, purity>99%, small packaging can reach 500g/bottle, trial packaging 50-100g/bottle, purity difference 99% -99.99% can be customized. Uses of Tris(hydroxymethyl)aminomethane: commonly used in biological buffer preparation, biochemistry and molecular biology experiments, etc., surfactants, PH regulators in cosmetics, vulcanization accelerators and intermediates of fosfomycin, etc. The range of use can be It is said to be one of the more extensive biological buffers. Natural demand is also relatively high. There are not many tris manufacturers in the country, because tris is very dangerous in the production process. The step of hydrogenation has made many enterprises and manufacturers daunting, and manufacturers producing tris products have encountered safety accidents one after another. , It also indicates to the chemical industry that the product is not easy to come from, so since 2018, the price of tris has been rising. Desheng Biochemical is a manufacturer specializing in R&D and production of tris. Now it has supplied more than 100 companies to form stable sales and exported to many countries. With a monthly output of 20T, stable supply of goods, superior quality, perfect after-sales service, and professional technical guidance, all of these allow Desheng Tris base to enter the market faster. Whether you are using it for your own use or doing trade export, Desheng Biochemical can meet your needs very well. Free shipping nationwide, covering 34 provinces and cities across the country, and provide full service. Another advantage of the manufacturer’s direct supply of tris is that the product price will be lower, and Desheng recently launched a year-end discount event, the order price is lower than that on the market, business friends who need this product should not miss it!

In an environment where the new crown virus is raging around the world, it is self-evident that how to quickly check and isolate positive infections can play a role in controlling the epidemic. In the current popular specimen collection and processing methods, EDTA, Tris, and Virus Transport Medias play an important role in different stages. Here is a brief review.   Nasopharyngeal swab: The sampler gently holds the person's head with one hand, inserts the swab into the other, inserts the swab through the nostril to enter, and slowly becomes deeper along the bottom of the lower bridge of the nose. Because the nasal tube is curved, do not use excessive force to avoid traumatic bleeding. When does the tip of the swab reach the back wall of the nasopharyngeal cavity, gently rotate it once (if a reflex cough occurs, please wait a while), then slowly take out the swab and dip the tip of the swab into the 2-3ml virus Test tube of preservation solution (or isotonic saline solution, tissue culture solution).   Stool specimen: Take 1ml of sample treatment solution and take a sample of approximately one size. The stool specimen treatment solution can be prepared in the laboratory: 1.211g tris, 8.5g sodium chloride, 1.1g anhydrous calcium chloride or 1.47g water containing crystals of calcium chloride, dissolved in 800ml deionized water. Centrifuge at 8,000 rpm for 5 minutes, absorb the supernatant and save it for use.   Anal swab: Gently insert a sterile cotton swab into the anus to a depth of 3-5 cm, then gently rotate and pull it out, immediately put the swab into a 15ml screw cap sampling tube containing 3-5ml Virus Transport Media, discard the tail and Tighten the tube cap.   Blood sample: It is recommended to use a vacuum blood vessel containing EDTA anticoagulant to collect 5ml blood sample. Nucleic acid extraction should be carried out in whole blood or plasma according to the type of nucleic acid extraction reagent. For plasma separation, the whole blood should be centrifuged at 1,500 to 2,000 rpm for 10 minutes, and then the supernatant should be collected in a sterile plastic tube with a screw cap.   Serum specimen: Collect 5 ml blood specimen collection tube with vacuum negative pressure blood. Place the specimen at room temperature for 30 minutes, centrifuge at 1,500-2,000 rpm for 10 minutes, and collect the serum in a sterile plastic tube with a screw cap. Other materials: Collect in a standardized way according to design requirements.

Under normal conditions, serum separation gel is used in extracorporeal blood testing, but many people do not know whether serum separation gel is harmful to the human body. In fact, it can be judged from several aspects to determine whether it is harmful to the human body.  First of all, we can first look at the components of the serum separation gel. The components of the serum separation gel are polyolefin, polyester or propylene, silica powder, thixotropic agents and stabilizers. After these materials are in contact with blood, the product's hydrolysis resistance is not harmful, but the ability to resist hydrolysis will have a certain impact on the contact materials. However, nowadays, serum separation gels with better hydrolysis resistance are generally used. Another aspect is to look at the use status of the serum separation gel. Everyone knows that the earliest serum separation gel is generally used in vacuum blood collection tubes to separate serum and blood clots. With time development, the current serum separation gel can also be used in vacuum blood collection tubes. Plastic surgery and medical treatment in some departments are targeted, but this is by extracting substances from the human body, and then using the extracted substances to backfill the human body. Used in blood collection tubes, it still has no effect because it will not touch the human body. But will the use for cosmetic surgery affect the human body? Because this method of use is different from the blood collection tube, it is separated by the serum separation gel, so that the platelets and serum proteins in the serum and blood clots can be better extracted, but the serum separation gel will come into contact with the extract during this process. Yes, and these substances have to be filled back into the human body at the end, so does this part of the substances have an impact on the human body? This type of separation gel has a professional name called PRP gel. It is a serum separation gel that is specially used to extract platelets and other components. The quality specifications are very high. Only when the quality requirements and biocompatibility meet the requirements will it not be used. Have a negative impact on the human body. It can be seen from the above that the serum separation gel basically has no effect on the human body. However, if you find that there are foreign bodies, abnormal odors, abnormal colors, etc. in the serum separation gel, or the effective use period has passed, try not to use it, because there is no guarantee whether it will affect the human body. Moreover, there are some differences in the process and raw materials between the manufacturers of serum separation gel. If there are multiple brands of serum separation gel, please do not mix them. This will cause the residue of the serum separation gel and affect the test results of the blood collection tube. Phenomena such as reaction of the contact material.

Today we will introduce TRIS base, HEPES, TAPS, the team products in the Good’s buffer produced by Desheng. TRIS (Trishydroxymethylaminomethane): Weakly alkaline, with a pKa of 8.1 at 25°C, and the effective buffer range of its buffer is between pH 7.0 and 9.2. It is often used as a buffer in electrophoresis and gel experiments, and is widely used as a solvent for nucleic acids and proteins. It can also be used for the formation of nematode intermediate fibers. The most common role in cosmetics is to adjust the PH value (pH). It can also be used as a neutralizer to neutralize a thickener (carbomer) to reduce stickiness, improve skin cell respiration efficiency, and prevent pore clogging . In addition, TRIS can be used as an odor regulator in cosmetics containing amine salts to adjust the amine odor generated by friction when applying cosmetics, so as to avoid any residual or sustained odor release. HEPES (4-hydroxyethylpiperazine ethanesulfonic acid): It is a zwitterionic biological buffer, belonging to Good’s buffer, with an effective buffer range of 6.8~8.2. It can be widely used in a variety of biochemical reactions and used as a buffer reagent in certain cell culture media. In addition, due to its non-cytotoxicity, HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. TAPS (Trimethylol methylamino propane sulfonic acid): It is also a zwitterionic buffer. In biochemical experiment projects, it can be used as an important pH stabilizing reagent. It is generally mixed with weak acid and its conjugate base to obtain an appropriate pH value. TAPS buffer reacts with most organisms under neutral conditions. The pH value is required to be selected in the range of 6 to 8. At the same time, the effective buffer range of the buffer should also be specified in the range of 6-8. Moreover, it is necessary to ensure that the pH form of the buffer reagent cannot chelate with certain metal chemical ions. In the field of clinical diagnosis, TAPS is also commonly used as a biological buffer. Usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA/RNA extraction kits and other kits.

In our nucleic acid examination, we will use our company's product: Virus Transport Media. In fact, the Virus Transport Media is also divided into multiple models, and there is also a twin brother: cell preservation solution.   Among them, the Virus Transport Media is roughly divided into two types: inactivated type and non-inactivated type. It can be understood from the literal meaning that there are differences in the preservation principles of the two types of Virus Transport Medias. Here is a brief introduction. The first non-inactivated type can protect the protein and nucleic acid of the virus, and the other is the inactivated type, which usually contains the lysis salt of the inactivated virus, which cleavages the protein to protect the nucleic acid. Among them, if the non-inactivated Virus Transport Media cannot be tested in time after sampling, it must be stored below 4 degrees Celsius and the preservation time should not exceed 48 hours. If it cannot be delivered to the test site within time, it needs to be Store in ultra-low temperature below minus 70 degrees Celsius. Otherwise, it will affect the virus test results, so you need to select the type of Virus Transport Media corresponding to the situation when selecting the Virus Transport Media. Many people confuse the Virus Transport Media with the cell preservation solution. In fact, the cell preservation solution is a general-purpose cell cryopreservation solution that can be used to preserve animal and human cell lines. Cell preservation is a cytology experiment. Important technical means. In cell line establishment and line establishment, it is very important to freeze the original cells in time.   At present, cell preservation mostly uses glycerol or dimethyl sulfoxide protective agent, which can improve the permeability of the cell membrane to water, and then slow freezing can make the water in the cell seep out of the cell, reduce the formation of ice crystals in the cell, thereby reducing Cell damage caused by ice crystals. The cell preservation solution has a diluting effect, which can isolate a large number of effective cells embedded in the mucus, allowing more valuable cells to be preserved, providing a sufficient number of cells, and providing a guarantee for the accuracy of the test results.   Therefore, although the purpose of the Virus Transport Media and the cell preservation solution are similar, there are some differences in principle. Virus Transport Media is preserved by protecting nucleic acid, while cell preservation solution is preserved by improving cell capacity and protection. These two preservation solutions should be able to distinguish between them after the above understanding.

Carbomer 940 is a kind of polyacrylic acid cross-linked polymer, mainly used for thickening of daily chemical products. It can achieve the effect at very low concentration. Carbomer 940 is usually used to increase the viscosity of cosmetics such as shampoo, shower gel, detergent, and some creams. Most daily chemical products contain this ingredient, so as a chemical product with such a wide range of applications, The price of Carbomer 940 is a general concern of buyers. As a necessary raw material for hand-washing gel, Carbomer 940 has been in short supply during the epidemic this year, and the price has fluctuated greatly. After the epidemic has gradually subsided, as more Carbomer 940 manufacturers are put into production In China, the price of Carbomer 940 is slowly returning to a stable level. As a domestic high-quality carbomer manufacturer, the price is very competitive, and the quality of Carbomer 940 is on par with imported products. Recently, the sales department of Desheng reported that many customers went offline after consulting the price of Carbomer 940 because they did not get a clear reply. They went offline without a trace. Here we must be fair to the customer service. Is the online customer service available? Knowing the specific order quantity, it is impossible to give a clear response. The 940 kg price of Carbomer is definitely different from the ton price. This is the same as retail or wholesale. Large quantities are more favorable. The same applies to the chemical industry, so it is very important to remember to clearly express the order requirements when consulting the price of Carbomer 940. In addition, if you want to find Carbomer 940 with high quality and low price, it is a wiser choice to find a factory direct seller. In order to seize the current carbomer market enthusiasm, many merchants purchase Carbomer raw materials at low prices and provide them to buyers. It has caused many unqualified phenomena after use, because the product is not developed and produced by itself. Once there is a quality problem, there is no after-sales guarantee. Desheng develops and produces carbomer by itself, which gives buyers a "boosting shot". It not only provides product quality assurance, but also solves customers' troubles. In addition, the price of Carbomer 940 is much lower than other merchants, so it is a very wise choice to choose Desheng. Desheng has always insisted on providing customers with high-quality, cost-effective products. If the quantity of Carbomer 940 you need is relatively large, you can enjoy price concessions. The current ton-level price is 130 yuan/kg. In addition, Desun can also provide free samples of Carbomer 940. You can follow other products on the market. For comparison, Desheng always believes that good products can withstand the test of the market. If you have a need, you can click on the official website to consult customer service.

In biological experiments, there is a very important pH stabilizing reagent, that is, the biological buffer CAPS. Usually in the experiment, an appropriate weak acid and its conjugate base will be selected and mixed to obtain a suitable pH value. It has been proved in a large number of experiments that most biological reactions occur under neutral conditions. Generally, the pH value is between 6-8, and the effective buffer range of the buffer is required to be between 6-8. Therefore, it is required that the acid-base form of the buffer does not chelate with certain metal ions. CAPS, also known as 3-cyclohexylaminopropanesulfonic acid, is a biological buffer, which can be used as a buffer for enzymatic chemistry and HPLC separation of alkaline drugs. Appearance is white crystal or powder, CAPS can be dissolved in water, PH buffer range is 8.9-10.3, PKA value is 9.6 when below 25℃, molecular weight is 243.28, CAS number is 1135-40-6. CAPS has a wide range of applications, including biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, as well as buffers for enzymatic chemistry and HPLC separation of alkaline drugs. Moreover, CAPS is also a raw material for manufacturing welding materials and air-conditioning equipment, as well as for manufacturing metal lithium. And CAPS also has many applications in industry, such as new coatings and new materials. When CAPS is used as a biological buffer, high purity is required, usually analytically pure. When used in industry, the purity requirements are relatively low, but different treatments are required for different applications. And CAPS has a very wide range of applications, so there are many relative manufacturers, and these issues need to be paid attention to when choosing a manufacturer. Desheng Bio has been engaged in the research and development and production of biological buffering agent projects for many years and has very rich experience. CAPS is one of the core products, and it is well received in the field of biochemical diagnostic reagents and the market of new coating products. Desheng has established a company for decades. The company's products are sold globally. At present, it has been sold to more than 100 countries around the world. The products are of good quality and more favorable prices. If you are interested, you can call for consultation. Desheng Bio welcomes your calls.

Virus Transport Media is the transportation and preservation medium of virus, chlamydia, mycoplasma and Ureaplasma samples. Avoid aerosol infection. It plays an important role in the research center of viral pathology. It is commonly used for the collection and transportation of clinical influenza, avian influenza, hand, foot and mouth disease, measles and other virus samples, as well as Mycoplasma, Ureaplasma, chlamydia and other samples. The basic matters of use are described below. handle: Before sampling, write the sample information on the sticker. According to the experimental purpose, samples were collected from the corresponding parts with cotton swabs. Immediately after sampling, put the swab back into the anti-corrosion solution, break the swab rod and tighten the cover. In the inactivated transport and preservation medium of collected specimens, the tubes can be transported to the laboratory at room temperature (5-25 ℃) within 7 days. Avoid repeated freeze-thaw cycles. For best performance, complete the test within 24 hours in a controlled temperature environment.   Specific sampling method: a) nasal swab: gently insert the swab head into the nose of the nasal canal, stand for a while, and then rotate slowly. Wipe the other side of the nostril with another swab, immerse the swab head in the preservation solution, break the swab rod and discard the upper rod（ Applicable to the sampling of this product) b) pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with the swab, immerse the swab head in the preservation solution, break the swab rod and discard the upper rod（ Applicable to samples of this product).   matters needing attention: Please read this instruction carefully before use. Do not use after the expiration date. Do not use if the original seal of the media and cotton swab is damaged. Before using the product, please check the outer package of the product to ensure that it is complete and free of content overflow. The label shall be clear and the content shall be clear without falling off. The stored solution shall be pre loaded into the sampling tube. Low temperature precipitation is a normal phenomenon. The transport and storage medium for biological inactivation is a transparent solution. Do not use it if the color of the medium becomes turbid or turbid; The transportation and storage medium for biological comma inactivation is orange red solution. If the color of the medium changes from light orange to red, do not use it.   Avoid contact with eyes and skin. Do not contaminate the tip of the swab before sampling. Be careful when inserting a cotton swab into the mouth for oral sampling. Avoid the risk of suffocation. For single use only. Different patients are prohibited from using the same swab or test tube. Searching for specimens of viruses, chlamydia and Ureaplasma involves the risk of infection, which must be collected with appropriate PPE and treated for biological risk in accordance with published guidelines and local regulations.   Storage: For optimum performance, the product must be stored in its original packaging at a temperature of 5-25 ° C until use. Do not expose to overheating or direct sunlight before use.   Desheng biochemical is committed to the research and production of additives in blood testing, including blood collection preparation, edat sodium salt, potassium salt, heparin, coagulant, separation gel, Virus Transport Media, Carbomer 940 / 980 hand sanitizer, etc.

Carbomer is a white, loose powder with strong hygroscopicity and soluble in water, ethanol and glycerol. It can be used as external emulsion, cream and gel. In the neutral environment, carbomer gel system is an excellent gel matrix. It is crystal clear and feels lubricated. It is very suitable for preparing cream or gel. At the same time, because of its simple process, good stability and comfortable feeling, it is widely used in topical administration, especially in skin and eye gel. Although it can be combined with water to form gelatinous transparent emulsion alternation, it is not directly dissolved in water like traditional inorganic salts. Today, we introduce an alkaline preparation method to prepare the ideal carbomer gel solution.   First weigh 1g carbomer 940 dry powder and put it into a 50ml dry beaker. If there is caking, please grind it with a spoon. Then add 25ml purified water and stir while adding to make carbomer 940 fully absorb water and dissolve. This step is very important. Carbomer 940 must fully absorb water. Generally, Carbomer 940 can fully dissolve and swell only after adding water and stirring for a few hours. If you want to be faster, you can heat it in a water bath. It will be faster to stir while heating. Of course, even if heating can accelerate the water absorption and swelling process of carbomer 940, it will take 10 ~ 30 minutes. Then use the drop tube to add alkaline solution, stir it side by side, and measure the pH value at the side. When the PH7 is adjusted, the gel is successful. Triethanolamine can be used as alkali solution). At the same time, make up the remaining water. The total volume is about 50ml.   The prepared gel base agent can directly mix various kinds of gels with other raw materials. As long as the concentration of carbomer is controlled within 0.8%, it is recommended to be suitable for about 0.5%. Only 0.5% of the concentration can produce gel products with good appearance and texture. For example, the gel made of 100ml is only required to take the polymer gel 25g with a pre modulation of 2% concentration into the formula of 75ml. 2% transparent gel 100ml = 2gm gel powder + 98ml pure water + 0.5ml antibacterial agent + moderate neutralizer. DIY carbomer 940 gel.   Special reminder: don't add things indiscriminately! Such as soluble salt, VC, highly acidic solution (lemonade, vinegar). In addition, the carbomer 940 gel system becomes thinner immediately. Hyaluronic acid is also acidic, but because of its small amount of addition, it has little effect on the stability of carbomer 940 gel system, and it can be safely added. Similarly, pure dew is slightly acidic and has little effect after addition.   Carbomer 940 itself is not nutritious, does not support the growth of bacteria and mold, but it can not prevent bacteria and mold from growing in the presence of nutrients in the gel system. So don't add too much at a time. Of course, you can add a little preservative or essential oil as needed.) Ultraviolet radiation has a certain effect on carbomer 940 gel system, so avoid light preservation. When preparing skin care products, the final concentration of carbomer 940 is controlled within 0.8%, which is recommended to be about 0.5%. If the concentration is greater than 0.8%, it is easy to form a film and the skin feels bad. Personal suggestion is about 0.7 beautiful, too thin is close to the thicker essence of liquid.   Desheng biochemical was founded in 2005, specializing in the R & D, production and sales of carbomer 940 / 980. Based on years of practice, it has formed a production and R & D capacity with independent intellectual property rights and professional. Provide products and raw material solutions for domestic and foreign daily washing and cosmetics manufacturers.

Carbomer is a highly hydrophilic anionic polymer. It is a white loose powder with strong moisture absorption. It can swell rapidly in water, but it does not dissolve. The aqueous dispersion solution is acidic, the pH value of 1% aqueous dispersion is about 2.5 ~ 3.5, and the viscosity is low. When neutralized by alkali, the viscosity gradually increases with the gradual dissolution of macromolecules, forming a clear solution at low concentration, forming a semitransparent gel at a relatively large concentration. PH 6.5 ~ 7 has the greatest viscosity and consistency. When the dispersing solution of polymer glue is neutralized with alkali, a transparent and stable gel is formed. How to quickly dissolve the white carbomer powder into water to make a transparent solution? From the German Sheng biological staff, here to explain to you directly do Kapo gel method, I hope to help you. Prepare the following three raw materials: a carbomer 940 1g pure water 99ml, B triethanolamine drops, C antibacterial agent 0.5ml. Tools: mixing rod, measuring spoon / measuring cup, pH measuring paper and an empty jar. Place the A in the empty jar and stir quickly until it is even. If there is a lump, it can be crushed with a stirring stick. Or only half of the water is added. The remaining part is added before the transparent gel is formed. Stand aside and stir until carbomer 940 fully absorbs water and expands without caking. It takes about 2 ~ 3 hours for the colloid to fully expand and form. If you want to shorten the colloid formation time, you can use the water separation heating method to stir while heating, which takes about 10 ~ 30 minutes Add the alkali to neutralize. Add a drop and a drop in the drop. Measure the pH value with the side stirring. At pH7, it is transparent gel. This step is very important. It is the key to the formation of transparent gel. When making the first time, it is best to add a drop of neutralizer - agitation - measure the pH value, repeat this step to pH value until 7, so that the technology of modulation can be easily grasped. Next, it is easy to make. Add antibacterial agent, stir evenly, gel finish. It should be noted that in general, about 1.35g of triethanolamine is required to neutralize 1g of polymer glue. Avoid compatibility with raw materials such as salts or acids, which will reduce its viscosity. It is stable in pH 4-10. Triethanolamine is an alkalizing agent used to adjust the pH value of the product. Avoid using with nitrosylating agent. The maximum use concentration is 2.5%. Avoid using it in the same formula with formaldehyde as preservative. May cause skin irritation and sensitivity. Desheng biochemical was founded in 2005, specializing in the R & D, production and sales of carbomer 940 / 980. Based on years of practice, it has formed a production and R & D capacity with independent intellectual property rights and professional. It provides products and raw material solutions for more than 100 manufacturing enterprises at home and abroad.

Chemiluminescence detection is a technique that allows detection with ultra-high sensitivity. The representative reagent is luminol acridinium ester. Chemiluminescence is a kind of luminescence phenomenon. After the substance absorbs external energy from electromagnetic waves, heat, friction, electric field or chemical reaction, it emits light of a specific wavelength without generating heat, and returns to the ground state from the excited state. When the source of absorbed energy is a chemical reaction, this phenomenon is called chemiluminescence. When light is absorbed by energy, this phenomenon is called photoluminescence, and this phenomenon includes fluorescence and phosphorescence. The light emitted by fireflies and deep-sea creatures is called bioluminescence. Figure 1 Chemiluminescence reaction caused by luminol Talk about the characteristics of chemiluminescence detection. Chemiluminescence does not require a light source lamp (such as a xenon lamp) because the material is excited by the energy of the chemical reaction caused by the luminescent reagent. Like post-column derivatization, the luminescent reagent used for the chemical reaction is continuously added to the column eluent by the luminescent reagent delivery pump, and mixed with the column eluent in the mixer. When the luminescence reaches the highest intensity, the photomultiplier tube measures the luminescence caused by the chemical reaction. Coil tubes made of ductile fluoroplastics are often used as chemiluminescence flow cells because it allows the photomultiplier tube to receive as much emitted light as possible. Examples of chemiluminescent reagents are as follows. There are two types of chemiluminescence reagents: direct luminescence reagents that are excited by the substance itself, and indirect luminescence reagents that stimulate another substance with energy from a chemical reaction. Chemiluminescence reagent. A typical example of this type of chemiluminescence reagent is luminol, which has been used to identify blood for many years. Chemiluminescence reaction caused by luminol. When a metal catalyst is present, luminol emits blue light (425 nm) in response to strong bases, oxidants (such as hydrogen peroxide) and heat. Luminol reaction can be used in HPLC to analyze peroxides (lipid peroxides, etc.) and metal-containing substances. Desheng Biochemical is a manufacturer of chemiluminescence reagents. It started in 2005 and has a private R&D team with 2 doctoral students and 6 master students. Provide consumers with chemiluminescent reagents with a purity greater than 99.3%, covering luminol/3-aminophthalic hydrazide/luminol, luminol monosodium salt, isoluminol/4-aminophthaloyl Hydrazine, acridinium ester DMAE-NHS, acridinium ester NSP-DMAE-NHS, acridinium salt NSP-SA, acridinium salt NSP-SA-NHS, acridinium hydrazide NSP-SA-ADH, acridinium ester ME-DMAE -NHS. If you have any needs, please contact us.