China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The cold winter is the season of "everything is sleeping, but I grow meat", and the meat that grows is only fat, that is, fat. If these fats only increase, it will only be the next summer to show off. So how is the fat in the body metabolized? Let us understand how acetyl-CoA synthase and acetyl-CoA oxidase are involved in fat metabolism.   The full name of fat is triglyceride or triacylglyceride. It is a polyester composed of long-chain fatty acids and glycerol. It is a substance that can store a lot of energy in the body. When the carbohydrates in the body are not enough to provide energy, fat will begin to gradually break down to provide energy for the body. Fat itself is insoluble in water, so it has better stability. It is not easy to participate in the reaction or decomposition at room temperature. It can be gradually decomposed under the action of various enzymes in the body. Acetyl-CoA synthase and oxidase are involved in fat metabolism   Hydrolysis of fats into free fatty acids and glycerin The triglyceride (triglyceride) stored in the adipose tissue is gradually hydrolyzed by lipase into free fatty acid (FFA) and glycerol, which is released into the blood for the oxidation and utilization of various tissues throughout the body, which is called triacylglycerol mobilization. One molecule of fat is hydrolyzed by lipase into one molecule of glycerol and three molecules of fatty acid. Glycerol enters into sugar metabolism and oxidizes to release energy, or gluconeogenesis is converted into sugar or glycogen.   Acetyl-CoA synthase activated fatty acid The free fatty acids obtained from fat hydrolysis enter the cell fluid and are activated by acetyl-CoA synthase (ACS) in the presence of ATP, CoA-SH, and Mg into fatty acyl-CoA. Fatty acyl CoA contains high-energy thioester bonds, and its water solubility increases, which significantly increases the metabolic activity of fatty acyl groups.   Acetyl-CoA oxidase breaks down fat Fatty acid is activated into fatty acyl COA and then enters mitochondria. Acetyl-CoA oxidase (ACOD) and thiolase in the mitochondrial matrix can decompose long-chain fatty acyl COA through four steps of dehydrogenation, water addition, dehydrogenation and thiolysis. Continuous reaction, cleavage of fatty acyl group produces 1 molecule of acetyl CoA and 1 molecule of fatty acyl CoA with two carbon atoms less than the original. This is repeated until it is completely oxidized to acetyl CoA, which is finally oxidized to carbon dioxide and water.   Acetyl-CoA synthase and acetyl-CoA oxidase are also used in the field of biochemical testing. The enzyme preparations of these two enzymes from Desheng can be used in kits, fatty acid testing, glycerol testing, and triglyceride testing.

TRIS (77-86-1) is an important organic chemical raw material, and it is also a common biological buffer. Because of its wide buffering range and good buffering effect, in current research, TRIS has been continuously expanding its application. Now there are many labels such as biological buffers, surfactants, vulcanization accelerators, and pharmaceutical intermediates. With more and more applications, the procurement demand will inevitably increase. So for buyers, what are the criteria for choosing a high-quality TRIS manufacturer? 1. High raw material procurement standards The production and development of any product is inseparable from the purchase of various raw materials. The quality of the raw materials is not high, so the quality of the products produced will be worrying. The same is true for tris. Recently, the raw materials for the production of tris TRIS have also seen a substantial price increase. For manufacturers, the proportion of raw material costs is relatively high, but Desheng will not because When the price of raw materials increases, buy low-priced, low-quality raw materials. Desheng always firmly believes that only by making good products can be recognized by customers. Desheng will review all raw material suppliers and also conduct corresponding inspections on raw materials. To ensure its reliable quality.   2. Strict assessment of staff A high-quality TRIS (77-86-1) supplier In order to ensure the quality of each batch of products, not only will the staff responsible for the production be carefully trained, but also the staff will be very rigorously assessed to ensure every production The processes can be executed perfectly to effectively ensure the quality of the product. The production of the product is originally a delicate work, and mistakes will occur if you are not careful, so strict assessment is very necessary. 3. Have good storage and transportation conditions The storage routines of chemical products have very strict requirements. They are stored according to different classifications. Tris TRIS is relatively easy to absorb moisture, so it should be stored in a sealed, dry place when storing. , Desheng’s current cardboard drums are packed with plastic inner bags, and the bottle mouths will be plastic sealed, so the tightness is very good. Coupled with the spacious warehouse for classified storage, TRIS has a good Storage conditions.   In general, as a high-quality TRIS (77-86-1) supplier, Desheng currently has a large number of cooperative customers, and the praise continues, because Desheng will use high-quality raw materials to produce TRIS, and strictly assess the production personnel , There are good storage and transportation conditions, and Desheng is a direct manufacturer, there is more room for profit in the order quantity conference, welcome to buy!

When biochemical reactions are carried out, a constant pH value is usually required. A good buffer can keep the pH value constant and reach a stable working condition. In the process of cell culture, the growth and metabolism of cells will cause drastic changes in pH value of culture medium. In order to maintain the stable growth environment of cells, it is necessary to add appropriate amount of biological buffer in the culture medium.   Here is a brief introduction to how to maintain the pH value of biological buffer solution, because the buffer is composed of weak acid (HA) and its conjugated base (a –) or weak base and its conjugated acid. Weak acids and bases can not be completely dissociated in water, but exist in the equilibrium state of dissociated and non dissociated species in buffer solution. For example, in acetic acid solution, there is an equilibrium of acetate ion, hydrogen ion and undivided acetic acid. Because of the dissociation of acetic acid (HAC), it can absorb H + or Oh -. HAC can release H + to neutralize Oh - and form water. Conjugated base a – can react with H + ions added to the system to produce acetic acid.   There are so many biological buffers and alternatives that can be used in cell culture on the market. Why choose HEPES? In fact, the suitable culture environment for most cells is ph7.2-ph7.4, while the application range of Desheng HEPES buffer just falls within ph6.8-ph8.2, which is in line with the characteristics of pH value needed for cell culture.   Compared with other buffers, HEPES has higher stability in maintaining the pH value of cell culture medium. Therefore, HEPES buffer is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell division, living cell imaging and other biological and biochemical research. Desheng is a professional biological buffer manufacturer with strong logistics capacity and rich export experience. We produce high-quality and high-purity HEPES buffer. We can provide a full range of product testing and special testing services according to your application needs.   In addition, we can also provide a variety of packaging specifications, HEPES purity ≥ 99%, good water solubility, stable process, can control the constant pH range for a long time. In any case, Desheng will be the best choice for you to buy HEPES!

Enzyme preparations are proteins with catalytic functions. They are biological preparations with special catalytic functions such as biochemical experiments and food processing. Nowadays, many users will search for keywords such as cost-effective enzyme preparation manufacturers on the Internet. The purpose of the search is to From the selection of more suitable products from these manufacturers, Desheng, as a professional manufacturer of enzyme preparations, will talk about what problems should be paid attention to when purchasing. 1. Choose enzyme manufacturers with diversified product types Currently, cost-effective enzyme preparation manufacturers can provide users with a variety of different types of enzyme preparations for users to choose from, including single enzyme preparations and compound enzyme preparations. Because enzyme preparations can break down single nutrients, such as cellulase, amylase or pectinase, protease and lipase or phytase, and complex enzyme preparations are made by mixing two or more of one preparation. As a result, Desheng has dozens of enzyme preparation products, which can meet different use needs. This is one of the main reasons for the large amount of cooperation between enzyme preparation manufacturers.   2. Choose an enzyme preparation manufacturer with a guaranteed effect Trustworthy enzyme preparation manufacturers will strictly abide by the corresponding procedures in the production process to ensure the use effect. If you don’t know how to check the effect, the best way is to refer to the standard of the authoritative company, as long as you use a unified standard to measure , You can make a comparison, and you can also ask the peers in the circle which manufacturer's products are more trustworthy. The effect of Desheng's enzyme preparation products is guaranteed. This is the biggest reason why most customers are willing to cooperate with them.   3. Understand the application of manufacturer's enzyme preparations Enzyme preparations are generally divided into several categories, including food and beverage enzymes, feed enzymes, etc., pharmaceutical enzymes and special enzymes (such as research and development reagents, etc.). The enzymes used in medicine have many types, low dosages, and high efficiency. Features: Desheng's enzyme preparation products are mainly used in biochemical testing and biochemical experiments. For example, glucose oxidase is mainly used to detect blood glucose concentration, cholesterol oxidase is widely used in clinical diagnosis of plasma total cholesterol content, and other experimental research. A variety of enzyme preparations are widely recognized at home and abroad.   In a word, the reason why Desheng's enzyme preparation products have such a large amount of cooperation is related to its ability to provide a rich variety of products, but also to the use effect of its products. You must pay attention to these aspects when choosing. Welcome customers with purchase needs to inquire and negotiate!

Heparin is common in clinical blood test with sodium salt and lithium salt, which has unique application value. Heparin has low chelating force, little influence on water molecule movement, less interference on blood components, no influence on red blood cell volume and no hemolysis. Therefore, heparin is recommended as anticoagulant in a variety of tests based on whole blood or plasma.   It is suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, hemorheology and emergency biochemical determination. In the detection of pH value, blood gas, electrolyte and calcium ion, heparin is the only anticoagulant that can be used, and heparin lithium has the least possibility of interference in the detection of non lithium ions, so it is recommended to use heparin lithium as anticoagulant. At present, heparin lithium is gradually replacing heparin sodium in blood test. Heparin lithium is a chemical substance and an important member of blood anticoagulant. The appearance is white to nearly white powder with CAS No. 9045-22-1. The titers were divided into 150u, 160u, 170u and 180u. Heparin anticoagulants commonly used include sodium, potassium, lithium and ammonium salts of heparin, among which heparin lithium is the first.   Application of heparin lithium anticoagulant 1. Biochemical test for patients after hemodialysis: the blood sample of patients after hemodialysis is a special sample faced by the laboratory departments of many hospitals, and many inspectors often feel helpless when facing such samples. This is because hemodialysis patients use anticoagulant drugs containing heparin, such as low-molecular-weight heparin calcium, etc. in the process of hemodialysis, because there is a certain amount of heparin anticoagulant drugs in the blood, it is very difficult to coagulate and separate the bleeding clear after blood extraction in the common tube or separation gel / coagulant tube.   It has been reported that the detection of blood K with heparin lithium anticoagulant plasma and serum is biased, which may be caused by the high rotation speed of blood sample during centrifugation, the red blood cells in the blood clot of fibrin silk mesh are crushed, hemolytic, and the K ions in the red blood cells are released to the serum. In addition, a small amount of red blood cells will inevitably be destroyed during the transportation and coagulation of blood samples, resulting in slightly higher blood K. It is also reported that there are significant differences in CK-MB, LDH, Glu between heparin lithium anticoagulant plasma and serum, which may be due to hemolysis or long storage time of blood glucose decomposition. On the contrary, heparin anticoagulant has the advantages of not affecting the cell volume and causing hemolysis easily. It does not need to be placed in a water bath and can be centrifuged directly. The blood K, Glu, LDH measured by heparin lithium anticoagulant plasma are more close to the real concentration of patients. Therefore, establishing the reference value system of plasma biochemical test as soon as possible is helpful for clinicians to make accurate judgment on patients' condition.   2. For routine biochemical tests: the results show that heparin lithium anticoagulant plasma can be used to replace serum in most routine biochemical test items, but the reference range of plasma should be established for Glu, K +, Na +, CI -, P3 -, and other items can be used to explain the results of plasma test with serum reference range; LP (a), PA, HBDH, LDH, CK, CKMB, IgM Plasma Lp (a) increased with the increase of heparin concentration.   Desheng has 15 years of rich experience in the development and production of blood anticoagulants. It is an old manufacturer of blood collection additives. The product has high purity of heparin lithium and heparin sodium, and the packaging can be made according to the needs of customers. Desheng biochemical insists on providing customers with high quality products and professional services.

Recently, a local case of the new crown emerged in Chengdu's Pidu District. In addition to the patient's partner, 11 of the 126 samples that have been tested in the environment and food samples such as door handles, switches, food in the refrigerator, and cutting boards in their homes were positive. This new coronavirus is so tenacious on items and foods other than people, is it necessary to use sample preservation solutions for in vitro preservation of virus samples?   First of all, after sampling the new coronavirus sample in nucleic acid testing, if it cannot be tested in time and needs to be transported or stored for a short time, the virus sample preservation solution must be used. Some people may think that the new coronavirus is different from other viruses. It is much more tenacious outside the body, and there is no need to use a virus preservation solution to store virus samples, but this is not the case. Terrible and tenacious virus Indeed, since the epidemic, the new crown has not only tested positive in humans and a few animals, but also tested positive in food, especially cold chain fresh food, and even frequent contact with product packaging, elevators, door handles, switches, etc. Some of the objects also tested positive. From this point of view, the virus is indeed very tenacious. This also highlights the complexity and severity of the epidemic prevention and control work, and the arduous nature of the prevention and control task, which has sounded the alarm bell for epidemic prevention and control.   However, it should be noted that although the virus is indeed detected on the object, and the positive rate of incomplete statistics is still close to one-tenth, it means that the virus can remain on the surface of the object for a certain period of time. Although there may be few remaining viruses and the retention time may not be long, there is still a relatively high risk of infection if people have frequent contact for a long time.   However, it is different for nucleic acid detection. The detection requires that the results are completely accurate. If the virus sample in the sampling tube is not added with preservation solution, there is a high probability that virus nucleic acid degradation will occur during transportation and storage. , Let alone one-tenth even if it can survive 99% in the sampling tube, even one may cause degradation and lead to false negatives.   When the sample in the virus sampling tube needs to be transported and stored, it is not only necessary to add a sample preservation solution. In order to meet the requirements of different detection conditions, Desheng also provides two types of virus transport media, inactivated and non-inactivated. Everyone must take protective measures on frequently touched objects.

At present, in many areas of the national nucleic acid testing, the swab virus preservation solution is added in advance in the sampling tube to prevent the virus degradation from causing false negative. Because many times the sampling point does not have the conditions for timely detection, the virus is very fragile, and the virus will degrade in a short period of time, leading to no detection.   On the other hand, a lot of Internet news makes us feel that the virus is very stubborn and difficult to eliminate. Before there was "oral disinfectant" farce, let us clearly know that drinking disinfectant directly can not kill the virus, but will cause harm to the body. There are also various so-called anti-virus tricks in China, such as chlorination of tap water, smoking, drinking, mask spraying disinfectant, sweat sauna disinfection and so on. All the above methods are wrong and can not kill the virus.   It seems contradictory that the virus is sometimes fragile or stubborn. To understand this problem, we need to have a basic understanding of the characteristics of the virus and the host cell of the virus.   Swab virus preservation solution Biological virus: The virus here refers to biological virus, which is composed of a nucleic acid molecule and protein or only composed of protein. The individual is small and the structure is simple. The common epidemic diseases are RNA virus, such as influenza virus, HIV and influenza A virus. The virus has no cell structure and can not replicate itself. Instead, it invades the host cell and replicates new virus with the help of the latter replication system.   Host cells of virus: Viruses can only live in the cells, but not outside the cells for a long time. Usually, animal viruses are parasitic on animal cells, plant viruses are parasitic on plant cells, and of course, viruses parasitic on fungi and bacteria. Cell is the basic structural and functional unit of organism. Whether it is animal, plant or fungus, it is composed of multiple or single cells. We all know that cell morphology is extremely small, usually need to use a microscope to see, we also know that the virus is very small, is a microorganism, but the specific relationship between virus and cell is very few people know.   The ratio of virus to cell size was as follows Although the cell is small, its internal structure is complex. There are various organelles in the nucleus and cytoplasm. If the virus is the size of a person, the cell is a super department store. The organelles are the various areas in the building. The virus is just a person hiding in a room in a certain area. If a simple drinking disinfectant or heating disinfection, it means that the cell building is destroyed first, then the cells and tissues and organs are destroyed.   The time of virus sampling is different. After sampling, the host cell of the virus rapidly decays, and the virus leaves the cell building, which is equivalent to throwing people into the wild without eating or drinking and dying soon. Therefore, it is necessary to add virus preservation solution to protect the virus samples collected by swabs.   After the epidemic subsided and the work resumed, Desheng immediately took action to ensure the supply of virus preservation solution in the market. There are two kinds of virus preservation solution for swab: one is the inactivated type, which protects the nucleic acid of the virus; the other is the non inactivated type, which protects the integrity of the whole virus. You can choose according to the needs of detection.

Desheng Biochemical Technology Co., Ltd. was established in 2005. After years of innovation and development, the company has now formed a national high-tech enterprise that is dominated by in vitro diagnostic reagent raw materials and integrates R&D, production, sales and service. Customers provide high-quality commissioned customization services and mass production services for scientific research products ranging from grams to tons. 2020 is coming to an end, and the train to 2021 is coming. Let’s take a look at this year’s journey. Sheng Technology's experience. 1. Under the epidemic situation, go against the trend On March 20, it became one of the first companies in Gedian to resume work during the epidemic, and cooperated with the delivery of medical protective equipment and guarantees the supply of diagnostic reagent raw materials, and contributed to winning the battle against the epidemic.   2. Assist in epidemic prevention and control, and rush to the world During the epidemic, successfully developed and produced virus preservation solutions, carbomers and other key products urgently needed by the epidemic. The quality and service have been trusted by many customers, and sales continue to rise step by step. Desheng’s inactivated and non-inactivated virus preservation Liquid assisted nucleic acid detection and contributed a meager effort to epidemic prevention and control. At the same time, it worked with foreign customers to help global epidemic prevention and control.   3. Carbomer project continues to exert strength Desheng conducted a small trial production of carbomer around October 2019. At the beginning of 2020, the company invested a lot of money to upgrade equipment and scale. After R&D personnel worked overtime, the final test of carbomer was completed after several tests. And the production capacity has been greatly improved, the daily output can reach 3-5 tons, and all the indexes of the produced carbomer raw materials are up to the standard, comparable to international brands. Its transparency is better than that of well-known brands. Behind the beautiful sales figures of the Kaboom project, it is inseparable from the full cooperation and close cooperation of all the company's manual personnel, and it is also inseparable from the technical production personnel who sacrifice their time and stick to their job responsibilities. https://www.vacutaineradditives.com/supplier-278018-blood-collection-tube-additives 4. National High-tech Enterprise The list of the first batch of high-tech enterprises in Hubei is based on the "Administrative Measures for the Recognition of High-tech Enterprises" (Guoke Fahuo (2016) 32 No.), the "Guidelines for the Management of High-tech Enterprise Certification" (Guokefa [2016] No. 195) and other relevant regulations, High standards and strict requirements are an effective proof of the company's scientific and technological strength and service capabilities, confirming that Desheng has passed the high-tech enterprise certification.   5. Normalization of epidemic prevention and control, restoration of diversified corporate cultural activities As the epidemic has entered the stage of normalization and prevention and control, the company’s corporate cultural activities have returned to normalization and diversification. Children’s Day, through charity and charity activities, sent holiday gifts and blessings to children in remote mountainous areas. At the same time, the company carefully prepared holidays for employees. Surprisingly, during the Dragon Boat Festival, a sachet DIY activity was carried out. While experiencing traditional festival customs, it also borrowed the beautiful meaning of "wear sachets to keep safe" to pray for an early end of the epidemic.   Looking at Desheng’s experience over the past year, there are laughs and tears. In the next step, the company will increase investment in research and development, and continue to transform research and development results into goals, continue to cooperate with universities to establish its own technology research and development center, and expand domestically and internationally. Business, use superb technology to give back to the market. The company will not forget the original intention, will continue to upgrade the technology, and continue to improve the quality of service! I believe that in 2021, Desheng will reach a higher level!

Heparin is a kind of mucopolysaccharide which widely exists in mammalian tissues. It mainly exists in mast cells and has anticoagulant effect. It is widely used in surgery and the treatment of cerebral thrombosis and myocardial infarction.   Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has attracted the attention of all countries in the world. It is also one of the main export drugs in China. With the deepening of research, it has been found that heparin sodium not only has anticoagulant, antithrombotic and lipid regulating effects, but also has anti-inflammatory, anti allergic, anti-virus, anti-cancer and other functions.   At present, heparin sodium is mainly extracted from small intestinal mucosa of pigs, sheep and cattle lungs. Research shows that heparin sodium is the highest content in small intestinal mucosa of pigs. Crude heparin sodium is a traditional export product of China and occupies an important position in the world. In this paper, the process of high sodium heparin extraction is introduced   (1) Raw material treatment: clean the fresh small intestine with water, scrape off the small intestinal mucosa after cleaning, and then put the small intestinal mucosa into a blender until it is mixed into a paste, and then set aside;   (2) Heating enzymolysis: put the chylous intestinal mucosa obtained in step 1 into a heating tank, add water and stir, then add 8% trypsin and weak alkali solution successively to adjust the pH value until the pH value of the solution is 8-10, and then heat it. During the heating process, the pH value should be kept between 8.5-9.5, heated to 30-40 ℃, and kept at constant temperature for 2-3 h, and then continue to heat to 50-60 ℃ and keep constant temperature of 10 -After 20 min, the filtrate was filtered while the temperature was high;   (3) Cooling and adsorption: the filtrate obtained in step 2 is cooled to 30-40 ℃, and then the floating oil on the upper layer of the filtrate is removed, and then the resin is added for stirring adsorption for 6-7h, and the resin is filtered out after the adsorption is completed;   (4) Elution: put the collected resin into the eluator for washing, add 10% sodium chloride solution, keep the temperature at 50-55 ℃, stir for 3-4 hours, collect the eluent; elute twice, combine the eluent collected twice for use;   (5) precipitation: filtrate the two collected eluent into the settling tank, add the alcohol with a mass fraction of 80-85% to stir evenly, then adjust the pH value to 7-8, and seal the precipitate for 10-12 hours.   (6) Dehydration and drying: put the precipitate in the drying oven to obtain heparin sodium. As a preferred scheme, the ratio of water to small intestinal mucosa in step (2) is 1:3. As a preferred scheme, the set temperature of the drying oven is 50-60 ℃, and the drying time is 3-5h.   In simple terms, the above extraction steps make the small intestine fully contact with trypsin by stirring the small intestine into chymose, and then adopt the process of enzymatic hydrolysis and heating to maximize the activity of trypsin, fully extract heparin sodium, and improve the yield of heparin sodium; in the precipitation process, the impurities are removed by filtration to obtain clean precipitates, so as to improve the purity of heparin sodium and improve the purity of heparin sodium To improve the quality of heparin sodium, Desheng is a professional manufacturer of heparin sodium. The potency is generally between 150IU and 180iu. We welcome all major manufacturers to consult and purchase.

Serum is an important sample for clinical biochemical detection. At present, the main way to obtain serum samples is to collect venous blood and centrifuge after blood coagulation. Under normal circumstances, blood samples in vitro need more than 60 minutes to complete coagulation, or even no coagulation, which is difficult to meet the needs of laboratory rapid detection. At this time, blood samples need to be processed to speed up the speed of blood coagulation. The commonly used method to promote blood coagulation is to add some coagulants, such as clay and cephalin, into the collected blood samples. When these coagulants are added into the blood appropriately, they can provide the active surface for coagulation factors to contact with foreign bodies and activate coagulation factors.   Several factors affecting the effect of coagulation promotion are as follows 1. Coagulation rate: blood coagulation mechanism is a process in which a series of coagulation factors are activated successively and finally form fibrin clots. Sometimes, there are filaments and lumps of fibrin in the vacuum blood collection vessel containing coagulant, which is caused by the lack of standardized use of coagulant promoting blood collection vessel.   In the preparation of vacuum blood collection vessel, the selection of coagulant with too fast coagulation speed will lead to the rapid contraction of fibrin, and the fragility of red blood cells will be broken, resulting in mild hemolysis. The proportion of blood clot is greater than that of serum, so the serum is under the upper blood clot, and the lower end of serum is still in contact with the upper end of blood cell. The cells can still use the nutrients in the serum to reduce the blood glucose measurement value, lactate dehydrogenase and serum potassium determination When using the gel accelerating tube, the specific gravity of the gel is higher than that of the blood clot. Therefore, the upper layer is the serum, the middle layer is the separation gel, and the lower layer is the blood clot. Therefore, the various components in the serum maintain physiological levels.   2. Coagulation temperature: the natural coagulation of blood is related to temperature. Blood can coagulate in a glass tube in a 37 ° water bath for 30 minutes. However, it should be pointed out that if the blood and coagulant are not fully mixed or the blood is not completely coagulated, it is easy to form fibrin gel like coagulation or fibrin filaments, whose specific gravity is smaller than the blood clot, so it remains in the serum layer and partially adheres to the separation gel. If the blood is directly used at this time, the blockage of the automatic analysis blood sampling needle can be caused.   3. Dosage of coagulant: the relative amount of coagulant needed to make blood reach the best coagulation effect. When the coagulant was used up on the next day, the coagulant was not prepared well on the next day. Fibrinogen in the blood gradually changes into insoluble fibrin under the action of coagulant. If the coagulation time is prolonged due to insufficient or ineffective coagulant dosage, centrifugation can lead to the precipitation of fibrin.   4. Whether the operation is standard or not: there are the following reasons for the precipitation of fibrin: the coagulant should be slightly reversed and mixed 4-5 times in order to make the coagulant evenly distributed in the blood, so that the center of the blood collection vessel and the surrounding blood coagulate at the same time. If the blood is not completely coagulated, that is, centrifugation can cause the precipitation of fibrin. Gel like and fragment like appeared in the upper part of serum and mixed with blood. Fibrin filaments are caused by the incomplete contraction of fibrin.   The coagulant should be sprayed quantitatively and dried under the condition of less than 45 ° C; if it is higher than 50 ℃, the coagulant effect may decrease. The coagulant prepared with distilled water or anhydrous ethanol should have standard quality management and be sprayed quantitatively. Only by using the coagulant tube containing neutralizing heparin to separate serum can high quality serum samples be separated correctly.   Desheng biochemical brand products for blood collection additives, has 15 years of R & D and production experience, coagulant, high efficiency coagulant powder, heparin sodium, heparin lithium, serum separation gel these products are our main products for so many years, high quality, stable performance, guaranteed after-sales service, welcome to exchange cooperation.

Luminol reagent and acridine ester reagent are commonly used in chemiluminescence immunoassay. Chemiluminescence immunoassay has replaced enzyme-linked immunosorbent (ELISA) as a more mainstream in vitro diagnostic method, accounting for more than 50% of biochemical detection methods.   The attraction of chemiluminescence immunoassay as an in vitro diagnostic technique lies in its simplicity. The essence of chemiluminescence is self luminescence, which means that the analytical instrument of chemiluminescence only needs to provide a method to detect the light signal and record the results. The autoluminescence detector needs a closed light sample chamber and a photo detector. The simplest is photo paper or X-ray, or even a visual detector. Chemiluminescence reagent The convenience of chemiluminescence detection method makes its application simple and completely automatic, but what is its sensitivity? Chemiluminescence has two inherent advantages.   1. Most of the samples have no background signal, such as they do not emit light.   2. Chemiluminescence detection is not a proportional test, which is different from fluorescence and absorption or colorimetric test. In the fluorescence test, it is very difficult to detect the fluorescent group with small Stokes shift, and the fluorescence is difficult to distinguish from the excitation wavelength.   Another problem is that some stray light will enter the detector, especially when the sample is turbid. In the absorbance test, the fundamental factor limiting the absorbance is to distinguish a small difference between two relatively strong signals.   It should be noted that the sensitivity of the detector to the spectrum of chemiluminescence is as close as possible, and the maximum sensitivity has been obtained. In general, the photomultiplier tube in autoluminescence instrument has the best response to blue light, and is not sensitive to the end spectrum of red light. The solid state detector has a good response to red light.   X-ray films are widely used for chemiluminescence imprinting analysis on nylon, cellulose or PVDF membranes. But we need to keep in mind that X-ray films are only used to detect light signals in the spectrum from ultraviolet to blue, although there are some special films that are sensitive to enhanced green light.   Chemiluminescence detection includes liquid samples and solid samples. The indicators of chemiluminescence detection are usually sensitivity, linearity and dynamic range. Desheng's chemiluminescence reagents include luminol, isoluminol and six acridine ester substrates.

Recently, the Office of the National High-tech Enterprise Recognition Management Work Leading Group Office of the Torch High-tech Industry Development Center of the Ministry of Science and Technology released the "List of the First Batch of High-tech Enterprises to be Recognized in Hubei Province in 2020". After a 10-working day public announcement, Hubei Xinde Sheng Material Technology Co., Ltd. successfully passed the certification with its excellent R&D and production technology.   The list of the first batch of high-tech enterprises in Hubei is combined in accordance with the "Administrative Measures for the Accreditation of High-tech Enterprises" (Guoke Fahuo (2016) No. 32), the "Guidelines for the Administration of High-tech Enterprise Accreditation" (Guoke Fahuo (2016) No. 195) and other relevant regulations The evaluation, high standards and strict requirements are effective proof of the company's scientific and technological strength and service capabilities, confirming that 71 high-tech enterprises including Hubei new desheng have passed the high-tech enterprise certification. https://www.vacutaineradditives.com/supplier-278018-blood-collection-tube-additives Hubei new desheng was established in 2017. It is a new technology-based enterprise established on the basis of Wuhan Desheng Biochemical Technology Co., Ltd. (2005). It specializes in the research and development, production and sales of diagnostic reagents. The main products are Vascular additives, chemical light reagents, biological buffers, enzyme preparations, new Trinder's reagents, antigen antibodies and other products. All products and services of the company are subject to targeted research, design and production according to customer requirements, and in terms of blood collection tube additives Formed with independent intellectual property rights and professional production research and development capabilities, has accumulated a wealth of knowledge and experience in the pretreatment of blood samples and has the advantages of professional technical services.   After 3 years of intensive cultivation, Hubei new desheng Material Technology Co., Ltd. has invested a lot of money and energy in product development, customer service and other fields. It has accumulated a lot of accumulation and successfully entered the list of high-tech enterprises to be recognized, marking that Desheng in vitro diagnostic reagents have entered the market. New step. Independent innovation is the life of an enterprise, and it is the foundation for the enterprise to climb the hurdles and grow stronger. As one of the few enterprises in the industry with independent factories, Desheng has a supporting independent research and development base and a high-quality, high-efficiency, high-yield Out of the R&D team.   The general manager of Hubei new desheng said that the approval of the first batch of high-tech enterprises to be recognized is the result of the unity and unremitting efforts of all employees. This is the country’s recognition of Desheng’s advanced technology, and Desheng’s commitment to customers A satisfactory answer sheet. In the next step, the company will increase investment in research and development, and continue to transform research and development results into goals, continue to cooperate with universities to establish its own technology research and development center, comprehensively expand at home and abroad, and use superb technology to give back to the market. The company will not forget the original intention, will continue to upgrade the technology, and continue to improve the quality of service!