China Wuhan Desheng Biochemical Technology Co., Ltd Company News

N,N-bis(hydroxyethyl)glycine, alias Bicine buffer, CAS number is 150-25-4, Bicine is a biological buffer; biological buffer is recommended for low-temperature biochemical work. Used to prepare a stable substrate solution for serum guaninase determination. It is also a very versatile Buffer, used for enzyme reaction buffer, electrophoresis buffer, working concentration from 3-100mM.Bicine is often used as a buffer solution for capillary electrophoresis. N, N-Dihydroxyethylglycine Bicine is a zwitterionic buffer, belonging to Good’s buffer, with a pH buffer range of 7.6-9.0. The molecular structure of Bicine is similar to that of glycine. The molecule has a substituted amino group, a carboxyl group and two hydroxyl groups as coordinating groups, so it has the properties of an amino acid and aminoethanol at the same time, and can form a metal complex with a single anion (Bic-) , Is often used as a buffer solution for capillary electrophoresis. Bicine is an effective inhibitor for the oxidative degradation of amines. According to the patent CN104245089A description, Bicine is an effective inhibitor for the oxidative degradation of amines, and can effectively inhibit the oxidative degradation of the amines used or the amine-containing absorbents used. Bicine is used as a chelating agent for soil remediation. Bicine is an amino acid analogue that is easily soluble in water. It is an environmentally friendly substance. It contains two -OH and one -COOH in its molecule. It has good chelating properties and can chelate heavy metal ions such as Cu, Cd, and Pb, but Can not chelate calcium and magnesium ions. Therefore, Bicine is often used as a chelating agent to repair soil contaminated by heavy metals. Bicine has a good elution ability for heavy metals under neutral and acidic conditions, and can efficiently remove Cu, Cd, and Pb ions in contaminated soil. Bicine is an eluent of active components, which can not only wash and repair heavy metals. It pollutes the soil, and can avoid the loss of plant nutrient elements calcium and magnesium in the soil, is safe and environmentally friendly, and will not cause secondary pollution to the environment. Bicine is also a widely used in vitro diagnostic reagent raw material, suitable for biochemistry and medicine. In the field of in vitro diagnostics, it is an important raw material for antibody detection reagents and nucleic acid detection reagents.

Before understanding Virus Transport Media, let’s first understand which viruses are made up of. Viruses are composed of a nucleic acid molecule and protein or only protein. Individuals are small and simple in structure. Common epidemic infectious diseases are usually RNA viruses, such as influenza virus, HIV, influenza A virus. Because there is no cell structure, the virus cannot replicate itself, but invades its genes into the host cell, using the latter's replication system to replicate new viruses. After the virus sample is collected, in order to maintain the viability of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be stored and transported in Virus Transport Media. Virus Transport Media has two types: inactivated and non-extinguishing. The inactivated Virus Transport Media uses high-concentration guanidine salt to quickly inactivate and preserve respiratory pathogens, making the sample lose infectivity. The inactivated samples can be used with a variety of viral DNA/RNA extraction kits. The M32/M96 nucleic acid extractor can quickly extract nucleic acids. At the same time, it can be used with the respiratory pathogen PCR detection kit to achieve rapid detection, and the specific sensitivity is not affected.     The non-inactivated Virus Transport Media contains Hanks liquid base, gentamicin, fungal antibiotics, BSA (V), cryoprotectant, biological buffer, and amino acids. A combination of multiple antibiotics has anti-bacterial and anti-fungal effects; Bovine Serum Albumin (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus; Hanks The neutral environment constructed by the buffer helps increase the survival time of the virus and the stability of infection. The non-inactivated Virus Transport Media is usually used for the collection and transportation of clinical influenza, avian influenza (such as H7N9), hand-foot-mouth disease, measles and other virus specimens, as well as mycoplasma, ureaplasma, chlamydia and other specimens. The sudden new crown epidemic has caused serious impacts on many companies. As high schools and junior high school students in major regions resume school, the demand for new crown pneumonia nucleic acid testing is increasing. Virus Transport Media, as one of the indispensable products for nucleic acid testing, has been greatly affected. Major companies are concerned. Since the resumption of work and production, Desheng Technology has been committed to the research and development and production of Virus Transport Media, blood collection tube additives and other products. It has made considerable progress and its products have been well received at home and abroad!

Depending on the degree of crosslinking and molecular weight, Carbopol has a variety of products of different types, including traditional series such as Carbopol 910, 934, 934 P, 940, 941, and 954, as well as new polymers such as Carbopol Ultrez 20/21 . Different types of carbomers have different uses in various industries due to their suspension stability characteristics, fluidity change length and other properties. Let's take a look at the properties and applications of the most commonly used carbomers in cosmetics.·Traditional carbomerCarbopol 934 (benzene solvent homopolymer)Appearance: white loose powder, slightly odorPerformance: short rheology; medium and high viscosity; medium transparency, slightly yellow; low ion resistance; high shear resistance; suspension stability, high heat resistanceSafety: There may be residual solvents (benzene or xylene)Application: Suitable for high-viscosity gels, lotions and ointments; suspension and emulsification; topical application; skin care; hair care; taste masking agent; cream; hand, body and face lotion. It is widely used in medicine (ointment) formulations and cosmetic creams.·Carbopol 940 (benzene solvent homopolymer)Appearance: white loose powder, slightly odorPerformance: (jelly type) very short rheology; high viscosity; high transparency; low ion resistance; low shear resistance; high yield value (suspension ability)Solubility: soluble in water, glycerin and ethanol, etc.Safety: No mold will grow when dry, non-toxic and non-irritatingApplication: It can provide the stability of the oil-in-water emulsification system and is suitable for use in lotions and creams.Suggested dosage: 0.2% ~ 1.5% · Carbopol 941/971 (benzene solvent homopolymer)Appearance: white loose powder, slightly odorPerformance: (snot type) long rheology; low viscosity; high transparency; medium ion resistance; low shear resistanceSolubility: soluble in water, glycerin and ethanol, etc.Application: It can produce low-viscosity permanent emulsions and suspensions, even ionic systems, and the resulting colloids have good transparency (more effective at low concentrations). Carbo 941 is used as an oil-in-water emulsification system in the cosmetics industry. Stabilizer, 971 is used as a slow-release agent in the pharmaceutical industry, suitable for transparent gels, hydroalcoholic gels and emulsion products.·※Improved carbomer (no benzene)Carbopol 980 (co-solvent homopolymer)Chemical name: Cross-linked polyacrylic resinAppearance: white loose powder, slightly odorPerformance: very short rheology; high viscosity; high transparency; low ion resistance; low shear resistance; high yield value (suspension ability)Solubility: soluble in water, glycerin and ethanol, etc.Application: Suitable for thickening, suspending and emulsifying in cosmetics or pharmaceutical formulations. Such as: styling gel, hydroalcoholic gel, moisturizing gel, shower gel, toothpaste, shampoo, shaving gel, moisturizing cream and sunscreen lotion, etc.Dosage: 0.2% ~ 1.5%·Carbopol 1342 (hydrophobic modified copolymer)Chemical name: Cross-linked polyacrylic resinAppearance: white loose powderPerformance: long flow characteristics; medium viscosity; high transparency; high ion resistance; high shear resistance; high yield value (suspension capacity)Solubility: soluble in water, glycerin and ethanol, etc.Application: Excellent rheology modifier in the presence of electrolyte, polymerization and emulsification, suitable for aqueous solutions or dispersions containing water-soluble salts.·Carbopol ETD 2020Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked CopolymerAppearance: white loose powderPerformance: long rheology; medium viscosity; high transparency; high ion resistance; shear resistance; efficient thickening, strong suspension abilityApplication: Suitable for transparent gels, hydroalcoholic gels, surfactant systems (special shampoos, cleaning products, etc.), and systems with high electrolyte content (such as aloe gel), etc.Suggested dosage: 0.2% ~ 1.5%·Carbopol AQUA SF-1New liquid acrylic rheology modifierINCI name: acrylic (ester) copolymerAppearance: milky white to white liquidPerformance: long rheology; high viscosity; medium transparency (the body is opaque, after adding the product); low ion resistance; high shear resistanceSolubility: soluble in water, glycerin and ethanol, etc.Application: It can be formulated into a clear formula, has excellent compatibility with a variety of ingredients, acidified and thickened, and can be used in surfactant systems.·Carbopol Ultrez 20 (new easily dispersible copolymer)Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked CopolymerAppearance: white loose powderPerformance: long rheology; high transparency; medium viscosity; medium ion resistance; high shear resistance; easy to disperse, excellent and stable suspension abilityApplication: It can be used in shampoos, shower gels, creams, lotions, and hydrogels with high electrolyte content. In addition, U20 can also give the product a bright appearance, with better fluidity and pourability, providing the product with a smooth, light, and soft touch.·Carbopol Ultrez 21 (new easily dispersible copolymer)Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked CopolymerAppearance: white loose powderPerformance: short rheology; high viscosity; high transparency; low ion resistance; shear resistance; easy to disperse, self-wetting, high-efficiency thickening, strong suspension ability, can give the formula a constant viscosity.Application: It can be used for gel, washing and cleaning products, high electrolyte products, etc.·Pemulen TR-1 (hydrophobic modified copolymer)Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked CopolymerAppearance: white powderPerformance: short rheologyApplication: Multifunctional thickening emulsifier, used as a stabilizer in oil-in-water systems, and can be used in creams and lotions.Suggested dosage: 0.2% ~ 0.4% to achieve 20.0% oil content.·Pemulen TR-2 (hydrophobic modified copolymer)Chemicals: acrylate/C10-30 alkyl acrylate cross-linked copolymerAppearance: white powderPerformance: long rheologyApplication: Multifunctional thickening emulsifier, used as a stabilizer in oil-in-water systems, and can be used in emulsions.Suggested dosage: 0.15% ~ 0.30% to achieve 60.0% oil content.

Desheng company is established for 20 years, focusing on R & D, production and sales in one of the old company, R & D and production of a complete range of products, price concessions, now on our company's R & D and production experience α- Glucosidase is a simple explanation. Glucosidase is one of the major enzymes in glycoside hydrolases (EC 3.2.1). It is named because it can hydrolyze glucoside bond and release a molecule of glucose. Glucosidase is one of the important members of glucose metabolism pathway. β- Glucosidase is involved in the metabolism of cellulose and many physiological and biochemical pathways, α- Glucosidase is directly involved in the metabolism of starch and glycogen. It has double functions of hydrolysis and transglycoside in the catalytic reaction of sugar. Hydrolysis can be carried out from α- Non reducing telostomy of glucosides, oligosaccharides and glucans α- 1,4 glycosidic bond, releasing glucose; In addition, the free glucose residues can be separated by transglycosylation α- Non fermentative Isomaltooligosaccharides (IMO) were obtained by transferring 1,6 glycosidic bond to another glucose or maltose substrate. The abnormal function of these enzymes will lead to metabolic diseases. At the same time, these enzymes are also the targets of many drugs and inhibitors to regulate the glycochemical metabolism in the human body. α- Glucosidase can be used to screen active natural drugs; one α- Immobilization of glucosidase: using trimethylol phosphorus as crosslinking agent and chitosan as carrier α- Glucosidase; 2. Make inhibitor screening model: immobilize the above-mentioned inhibitors α- The glucosidase was loaded into a column with a diameter of 0.8 cm and a length of 8 cm, in which the pH 6.8 potassium phosphate buffer was added and stored at 4 ℃; 3. Validation of the model: a representative α- Acarbose, a glucosidase inhibitor, was used to verify the effectiveness of the screening model. The lower end of the column was tightly inserted into a small tube with a piston, and a syringe was inserted into the upper end. The buffer solution in the column was pressed to about 0.4cm below the top of the column, and then 50 μ L 4-nitrobenzene- α- D-glucopyranoside (0.116mol / L) and a certain amount of acarbose solution (250mg / L), gently shake to make the solution at the top of the column mix well, then continue to press the solution until the liquid level is level with the top of the column, close the bottom piston, then put the column into the water solution tank, incubate at 37 ℃ for 10 minutes, after the reaction is completed, use 5ml Wash the column with ph6.8 potassium phosphate buffer, collect the washing solution, dilute the eluent to 10ml volume, and determine the absorbance value at 400nm with ultraviolet spectrophotometer; And the above 50 μ L 4-nitrobenzene- α- D-glucopyranoside, acarbose solution and 5ml buffer solution were diluted to 10ml as blank, and the inhibition rate of acarbose was calculated according to the absorbance data of the obtained reaction solution; 4. Immobilization α- Screening model of glucosidase for water soluble fraction of traditional Chinese medicine Polygonum cuspidatum α- Objective to screen the water-soluble fraction of Polygonum cuspidatum. We Desheng R & D and production of enzyme preparations used in the kit, customers with relevant needs are welcome to consult.

Carbomer, also known as carbopol, is actually a powerful polymer hidden behind this seemingly simple name. It is carefully cross-linked with acrylic acid and acryloyl sucrose or acryloyl pentaerythritol, and this unique structure endows it with a series of remarkable characteristics.   Firstly, Carbomer has demonstrated outstanding performance in the field of emulsification. It can easily emulsify oily liquids at a temperature of about 25 degrees, which is a remarkable achievement in the entire chemical industry. Even more surprising is that the entire emulsification process takes a very short time, which greatly improves production efficiency and reduces production costs. This efficient and convenient emulsification ability has enabled Carbomer to be widely used in fields such as cosmetics, pharmaceuticals, and personal care products.   In addition to outstanding performance in the field of emulsification, Carbomer also holds an important position in the skincare industry. As an excellent stabilizer, it can effectively maintain the stability and durability of skincare products. Adding carbomer to skincare products not only makes the product more stable, but also enhances its moisturizing effect, making the skin smoother and more delicate. In addition, carbomer also has good permeability and biocompatibility, which can quickly penetrate deep into the skin, providing sufficient nutrition and moisture for the skin.   In the Carbomer series, the Carbomer 940 and Carbomer 980 are two highly anticipated products. In contrast, Carbomer 940 has better permeability and viscosity, which makes it more advantageous in certain specific applications. For example, when it is necessary to prepare high concentration gel, Carbomer 940 can easily cope with it and show excellent performance. However, if you need to prepare 75% free gel, Carbomer 980 will be a better choice. Due to the relatively narrow applicability of Carbomer 940, it is usually only suitable for concentrations below 65%.   As a professional manufacturer of Carbomer 940 and 980, Desheng Company ensures excellent product quality with its advanced production technology and strict quality control system. The company not only provides high-quality Carbomer products, but also professional technical consulting and solutions for customers. Whether you are a cosmetics manufacturer, pharmaceutical manufacturer, or personal care product enterprise, Desheng Company can provide you with Carbomer products and technical support that meet your needs.   In summary, carbomer, as a powerful polymer, plays an important role in fields such as emulsification and skincare. The Carbomer 940 and Carbomer 980, as outstanding ones, have received widespread attention and application. If you are interested in Carbomer products or have any questions, please feel free to contact Desheng Company, and we will be happy to serve you!

4-Hydroxyethylpiperazine ethanesulfonic acid (HEPES buffer) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research.     As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products:   1. Advantages of PH range:   The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture.   2. Purity advantage:   Desheng HEPES has a purity of ≥99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services.   3. Advantages of reserves:   Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment.   4. Export advantages:   Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention.   5. HEPES packaging advantages:   We can pack in quantities according to customer needs. Generally, there are 500g small bottles and 25kg cardboard drums. The big packaging is covered with plastic bags, the white powder is packed in the belt, and the tie is tight.   6. Recommended storage:   HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not degrade due to autoclaving. However, if the HEPES aqueous solution is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H2O2) will be produced. Therefore, it should be noted that the HEPES aqueous solution must be kept away from light to avoid affecting the experimental results. HEPES powder is generally placed in a dry room when it is placed, and cannot be exposed to direct sunlight for a long time. It is best to store it at room temperature 2-8°C.   Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.

The full name of EDTA-2K is dipotassium ethylenediaminetetraacetate. It is a white crystalline powder and belongs to a common complexing agent. Because it can complex with the calcium in the blood to inactivate the calcium and further cause the blood to not coagulate, it is usually used to prepare anticoagulants and mainly used in the blood collection tube industry. EDTA has two CAS numbers, mainly because one of them is actually EDTA dipotassium crystalline hydrate. People often mistake the CAS numbers of these two EDTAs, which makes people confused. The real situation is that the CAS number of EDTA-2K is 2001-94-7 and the CAS number of EDTA-2K dihydrate is 25102-12-9. The difference between them is that there are two crystal waters in one molecule. And this article is an introduction to the wide range of uses of EDTA-2K. The International Blood Standards Committee recommends the use of EDTA-2K anticoagulated blood for complete blood counts. The use of EDTA-2K as an anticoagulant in vacuum blood collection tubes can preserve leukocyte hypertrophy in a short time, prevent platelet aggregation, and preserve platelets. In addition to being an anticoagulant, EDTA-2K has many uses in industrial production, environmental optimization and other fields. EDTA-2K can be used as a new type of gel material magnesium phosphate cement retarder, compared with borax used in traditional magnesium phosphate cement. It has a better retarding effect, can prolong the setting time of the magnesium phosphate repair system, and ensure the strength of the magnesium phosphate cement, making it more widely used in the rapid repair of highways, bridge decks, and airstrips. In oilfield production, EDTA-2K is also used as a stabilizer to form a desulfurization synergist, which can not only maintain the stability of the desulfurization synergist, but also chelate the calcium and magnesium ions in the operating system to be treated to stabilize the overall operating system. Water quality. The desulfurization synergist can effectively chemically react with the sulfide in the equipment to achieve the purpose of removing sulfide, have a good desulfurization effect, and ensure the safety of oilfield construction. EDTA-2K is also commonly used to prepare deodorants, such as toilet deodorants, decomposing pesticides and odor molecule removers, etc. In addition, according to the description of related patents, the potassium salt of ethylenediaminetetraacetate is calcined at high temperature, pickled, and washed. Activated carbon can be prepared in processes such as washing and drying.

There are many kinds of blood anticoagulants in blood collection tube reagents, and one anticoagulant has a wide range of uses, that is, potassium EDTA. There are many types of EDTA potassium salt, among which dipotassium is used more, but what about its principle and application? There should be some people who are not very familiar with it. Here we can take a look at what application areas and principles it has.     EDTA dipotassium salt is also known as dipotassium ethylenediaminetetraacetate dihydrate (EDTA-K2). It is a white crystalline powder, easily soluble in water, easy to absorb moisture, and has a molecular weight of 404.6. DETA dipotassium salt can protect the cell components of the blood, does not affect white blood cells, has the least impact on red blood cells, and can inhibit the aggregation of platelets, which is suitable for general blood tests. But for the separation test with platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor is it suitable for calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine amino peptide Enzyme determination and PCR test.   The dipotassium EDTA is mostly used in complexing metal ions and separating metals, as well as washing powder, shampoo, liquid soap, agricultural chemical spray, blood anticoagulant and other fields. The dipotassium EDTA is also recommended by the International Committee for Standardization in Hematology as the preferred anticoagulant for blood count and classification.   The principle of EDTA dipotassium salt as an anticoagulant, first of all, we first understand the principle of blood coagulation, which can be divided into three parts, 1. The formation of prothrombin activator, 2. The role of prothrombin activator in calcium ion With participation, prothrombin is converted into active thrombin. 3. Soluble fibrinogen is converted into insoluble fibrin under the action of thrombin. Fibrin is shaped like filaments, criss-crossed, and collects a large number of blood cells to form a jelly-like blood clot. This is how coagulation occurs. The dipotassium EDTA has a great affinity for calcium ions in the blood, and can complex with calcium ions so that prothrombin cannot be converted into thrombin, thereby making the blood anticoagulant.

Recently, some customers reported that the EHSPT (also called TOOS) solution prepared by myself was transparent, but it turned yellow or red after being left for two days. What is the reason? Can it still be used? Reasons for discoloration of Trinder's reagent solution: Chromogenic substrates such as EHSPT and MAOS are all new Trinder’s reagents, which are all derivatives of highly water-soluble sodium aniline, which will produce red or yellow substances when oxidized. Trinder's reagent powder is not easy to be oxidized. It needs to be sealed and refrigerated after preparation. If the solution is exposed to the air for a long time and the temperature is high, they may be slowly oxidized by the oxygen in the air. Discoloration of the solution occurred. Trinder’s reagent for enzyme-linked immunoassay Trinder's reagent detection principle: Trinder's reagents such as EHSPT and MAOS can be oxidized by hydrogen peroxide (TOOS, MAOS, etc. need to be coupled with 4-AAP) in the presence of peroxidase to produce red quinone imine substances, which can be detected by measuring absorbance with a photometer The content of the test sample that can generate or oxidize hydrogen. The new Trinder’s reagents are all reductive color-developing reagents. They are originally colorless. When they are catalyzed by peroxidase by oxygen, they can generate colored oxidation products. However, chromogen substrates such as EHSPT are relatively unstable in the solution. They are contaminated by oxidizing impurities or exposed to air for a long time, which will cause them to slowly oxidize, and the solution will change color. Therefore, when the chromogen substrate is formulated into a solution, it is generally recommended to prepare it for immediate use to prevent it from being contaminated or oxidized. The advantages of Trinder's reagent compared to TMB: When using TMB as a color reagent, the detection wavelength is 450nm maximum absorption wavelength and 405nm wavelength, and the absorption wavelength of bilirubin in the serum is 380~530nm, which will interfere with the detection results, and the color should be done under acidic conditions. Reaction limits its application. The UV absorption of the color reaction product of the new Trinder’s reagent is >540nm, MAOS and MADB are even as high as 630nm, and the reaction requires a wide pH range, and is less interfered by other substances in the serum. It can be used for detection items that require high precision values. At present, in addition to chemiluminescence immunoassays, enzyme-linked immunoassays are more commonly used. The new Trinder's reagent is one of the important chromogenic substrates. Desheng is engaged in the R&D, production and sales of acridinium ester chemiluminescence reagents and new Trinder's reagents. All have extensive experience.

In biochemical testing, the detection of blood glucose, blood lipids, liver and kidney function, etc. usually adopts enzymatic photometry. Many detection principles are based on Trinder reaction. The required chromogen substrates and enzymes have requirements for the experiment, and the stability is not ideal. Polymer stabilizers to improve.   The chromogen substrates used in the Trinder reaction, such as TOOS, MAOS, ADPS, etc., belong to the sodium salt of aniline sulfonate. After being formulated into an aqueous solution, it cannot be exposed to the air for a long time and will be slowly oxidized. Therefore, it is usually stored as a powder reagent. Temporarily prepare a solution. On the other hand, enzymes have higher requirements on the temperature and pH of the reaction system, and they are prone to inactivation even when the concentration is too low. So improving their stability is very valuable.   Chromogen substrate MAOS powder   Through research, it is found that a polymer with a special structure can significantly improve the stability of chromogen substrates and enzymes. The polymer has the general structural formula of R1 R2 R3, R1 is selected from olefin unsaturated groups or olefin unsaturated monomers; R2 is selected from ester group COO or other group O; R3 is selected from polyethylene glycol, polyoxypropylene, Polypropylene glycol, polyacryl alcohol and other polymer groups.   The application of this polymer can increase its stability by interacting with enzymes and chromogen substrates, so that a variety of in vitro testing items can maintain the stability of enzymes and chromogen substrates under extreme environments (such as high temperatures). The detection result has less deviation and higher accuracy. It can be combined with other components to form a stable enzyme and chromogen substrate composition. It can be used in enzyme-catalyzed color reaction and in the preparation of in vitro detection products based on the principle of enzyme-catalyzed color reaction. And the preparation of enzymes and chromogen substrate stabilizers.   Applicable enzyme preparations include: peroxidase such as POD or HRP, cholesterol esterase, cholesterol oxidase, alkaline phosphatase, creatine kinase, glycerol kinase, phosphoglycerol oxidase, lipoprotein lipase, urate oxidase, acet One or two or more of acid oxidase, glucose oxidase, creatinine hydrolase, and creatine hydrolase.   Trinder's chromogen substrates suitable for polymer stabilizers include: TOOS, TOPS, MAOS, MADB, etc. and their combinations 4-AAP, MBTH. The above-mentioned chromogen substrates and enzymes are commonly used reagents in enzymatic photometry. Desheng is a manufacturer and can provide a variety of chromogen substrates and enzyme preparations.

The full name of Tris-Hcl in Chinese is Tris Hydrochloride, another name is Tris Hydrochloride, and the English name is TRIS hydrochloride. I believe that many people are familiar with Tris. Tris has a wide range of applications. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses.     Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fibers of the nematode nuclear laminin. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tris is not suitable. Try to replace it with a buffer that can be tolerated by other mass spectrometers.   Tris-HCl is derived from the reaction of Tris as the main raw material. Its solution is acidic and is mainly used as a biological buffer to adjust the pH of the virus preservation solution. If you need to adjust to neutral or alkaline, you can add Tris or NaOH to achieve. In addition to this information, what are the configuration methods for Tris-Hcl? How much do you know? The configuration method of Tris-Hcl will be introduced below.   Preparation method of Tris-HCl buffer:   1. Tris-Hcl (0.05mol/L, 25℃) After mixing 50ml 0.1mol/L tris (Tris) solution with x ml 0.1mol/L hydrochloric acid, add water to dilute to 100ml PH    x/ml     PH     x/ml 7.1    45.7     8.1    26.2 7.2    44.7     8.2    22.9 7.3    43.4     8.3    19.9 7.4    42.0     8.4    17.2 7.5    40.3     8.5     14.7 7.6    38.5     8.6      12.4 7.7    36.6     8.7     10.3 7.8     34.5    8.8     8.5 7.9     32.0    8.9     7.0   Tris molecular weight=121.14: 0.1mol/L solution is 12.114g/L. Tris solution can absorb carbon dioxide from the air, so the bottle cap should be tightly closed when using. When configuring Tris-Hcl solutions with different concentrations and different pH values, you can first prepare a 0.05mol/L solution with a certain pH value, and then dilute to the required concentration.   2. 1M Tris-Hcl (PH7.4, PH7.6, PH8.0) Component concentration 1M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 121.1g Tris and place it in a 1 L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Add the required PH value for the adjustment of the concentrated HCL amount according to the following value; PH value              Concentrated HCL 7.4                        Approximately 70ml 7.6                        about 60ml 8.0                        about 42ml 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving. 3. 1.5M Tris-Hcl (PH8.8) Component concentration 1.5M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 181.7g Tris in a 1L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Adjust the PH value to 8.8 with concentrated Hcl; 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving.   It should be noted that the solution should be cooled to room temperature before adjusting the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.   The above is the configuration method of Tris-Hcl provided by Hubei Xindesheng Material Co., Ltd. In addition to the production of Tris-Hcl, Desheng also produces other chemicals, such as blood collection tube additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer products. At present, Desheng company has been established for decades, has its own R&D team, and has invested a lot of energy in biological buffers. The quality of the products is reliable, the price is favorable, and if you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Since the COVID-19 pandemic, COVID-19 reagents have been on fire, and a little brother, the COVID-19 preservation solution, has been brought along with him. "Suddenly like a spring breeze coming all night", the preservation liquid also springed up like bamboo shoots after a rain. However, "squandering flowers are gradually becoming charming." Faced with hundreds of products, there is really no way to choose. Which points should I look at? 1. Are the raw materials of the virus transport medium qualified? Highly responsible virus preservation liquid manufacturers can not only fulfill their promises, but also ensure that the facts are described without exaggeration, such as the strict selection of raw materials to prevent fraud. The irresponsible manufacturers will only exaggerate in order to promote the order. In this process, customers should keep their eyes open and distinguish right from wrong.   2. Is it easy to grow bacteria? After the preservation solution is stored for a period of time, it is easy to spoil. The clear preservation solution becomes muddy, moldy, and bacteria grow, and it is embarrassing to be unsafe at night. The bacteria that grow out are not bad, I know they can't be used. Those who have been contaminated but will not grow long, hide in which box, which patient they are used on, and what impact they will have on the results, is known to all. Preventing pollution is undoubtedly a big challenge in the production and quality control process. Of course, it is not difficult to deal with, and measures such as cleaning the workshop and adding antibiotics can all be dealt with calmly.   3. Is it easy to leak? Because the virus preservation liquid is liquid, some preservation liquids are prone to leakage when they encounter rough handling or the negative pressure of air transportation during transportation, so the entire box and the whole box have to be scrapped. If the packaging is not in place and leaks, it will be very troublesome. Therefore, the selection of packaging materials, the design of the structure, and the manufacturing process must be checked.   4. Is the price reasonable? The price of any product is a very critical factor. Because of the epidemic, many companies’ conventional products are not selling well. Therefore, many companies in the market will sell shoddy products at low prices in order to survive. The price is low, but the quality is not guaranteed. The price and value are often corresponding. If the price of the virus transport medium is much lower than the market average price, then you must seriously consider whether its quality is reliable. Therefore, when choosing a virus transport medium manufacturer, be careful not to blindly pursue low prices.