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Latest company new about Will the pH of TRIS-HCL buffer change with temperature?
2020/09/24

Will the pH of TRIS-HCL buffer change with temperature?

The TRIS-HCL buffer is stable. It can be widely used in the preparation of buffers in biochemistry and molecular biology experiments. It has good compatibility with physiological body fluids and will not form precipitates with calcium and magnesium ions. On the contrary, phosphate and calcium and magnesium ions will produce precipitation. Moreover, the ionic strength of TRIS-HCL buffer with the same pH and same concentration is lower than that of phosphate buffer. This is particularly important for enzyme determination. Because of the high ionic strength, some enzymes are easily inactivated. Therefore, sometimes using TRIS-HCL instead of phosphate buffer, the effect is better.   However, the pH of TRIS-HCL buffer is greatly affected by temperature. Once the temperature changes, the pH will change significantly. The problem of causing changes in enzyme activity has not attracted enough attention from the clinical laboratory. For this reason, we measured the temperature coefficient of TRIS-HCL buffer and discussed its practical value. The Tris reagent used in the following experiment was developed and produced by Tecsun Technology. Desheng TRIS buffer packaging Experimental process: Put the TRIS-HCL buffer in a 37℃ water bath for 30 minutes. Distilled water, the calibration solution remains at room temperature. Place the temperature knob at 37°C, and take out the buffer after equilibrating the temperature. Adjust zero with distilled water at room temperature, calibrate with mixed phosphate at room temperature (pH 6.84 at 37°C), and immediately determine the pH of the TRIS-HCL buffer. The buffer is placed at room temperature until the temperature drops to 23°C. Adjust to zero with distilled water at room temperature. Calibration of mixed phosphate at room temperature (pH 6.86 at 23°C), and then immediately determine the corresponding pH. The solution was kept at room temperature. Wait until the temperature drops to room temperature. Adjust the temperature knob again. Calibrate and determine the pH at room temperature.   Experimental results: According to the above method, the pH at 37°C is on average 0.18 lower than the pH at 23°C. The pH is 0.32 lower than the pH at l2℃ on average. Temperature changes have a great influence on the pH of the buffer, so it must be prepared at the operating temperature. Tris-HCl buffer prepared at room temperature cannot be used at 0℃~4 ℃.   Therefore, the pH of the TRIS-HCL buffer is greatly affected by temperature changes. When measured by different methods, the changes are different. The pH changes of the two temperatures (37°C, 23°C) have nothing to do with the measurement method. We speculate that to determine the pH value of a solution at a certain temperature. It is necessary to adjust not only the temperature compensation value of the pH meter but also all the various solutions and distilled water to the measurement temperature.
Latest company new about What is the difference between the blood anticoagulant heparin calcium and heparin sodium?
2020/09/21

What is the difference between the blood anticoagulant heparin calcium and heparin sodium?

There are many types of heparin salt blood anticoagulants. Heparin sodium is the most commonly used, but heparin calcium is used in some places. Is there any difference between heparin calcium and heparin sodium in blood anticoagulation?   Heparin sodium is used for anticoagulant drugs and other non-drug reagents such as blood collection tubes or cosmetics. The potency, purity and impurity content of heparin sodium for different purposes are different; while heparin calcium is mainly used for For drugs, the requirements are relatively high.   Heparin sodium and heparin calcium are both used as blood anticoagulants, and the principle of action is similar. Both use heparin to anticoagulate. Unfractionated heparin and low molecular weight heparin can be combined with antithrombin iii to increase the affinity of antithrombin iii and coagulation factors, and play the role of anticoagulation factors.   Low-molecular-weight heparin has two forms, sodium salt and calcium salt, but the clinical efficacy is not much different. Heparin calcium is slightly stronger than heparin sodium in anticoagulant factor 2a, and the anticoagulant effect is relatively weak, but overall there is no significant difference in clinical efficacy.   Heparin sodium is easy to cause subcutaneous hemorrhage when injected subcutaneously, and local stimulation of heparin calcium is slightly lighter than heparin sodium when injected subcutaneously, which can effectively avoid this adverse reaction.   In the beginning, there was only heparin sodium, but later it was discovered that its bioavailability was relatively low, and there would be local adverse reactions. For example, when choosing subcutaneous injections around the umbilical area, ecchymosis may appear. In severe cases, the bioavailability of heparin calcium is relatively high, and the absorption is relatively fast. Local adverse reactions, especially subcutaneous ecchymosis, are relatively rare.   Heparin sodium is generally used for needle sealing for infusion patients, and its effect is quite good. However, when patients choose subcutaneous injection, it is better to choose heparin calcium, so the adverse reactions will be smaller.   Regarding how to choose heparin sodium and heparin calcium, the specific use should be under the guidance of a specialist, and an individualized medication plan should be made according to your own situation. What needs to be reminded is that Desheng brand heparin sodium is not a medicine and cannot be used as a medicine. It can only be used as an anticoagulant for blood testing in blood collection tubes.
Latest company new about Which blood collection tubes are suitable for serum separating gel?
2020/09/20

Which blood collection tubes are suitable for serum separating gel?

Serum separation gel is a viscous fluid with thixotropy. Through centrifugation, the separation gel will form a colloidal isolation layer between blood cells and serum, thereby preventing the mutual diffusion between serum components and blood cells. The layer enables the serum to be analyzed, tested and stored in the original state as much as possible, to ensure the original properties of the blood sample, and thus greatly improve the accuracy of the test results. So, can serum separating gel be applied to those blood collection tubes? Next, the editor of Desheng will answer for everyone.   1. Nucleic acid detection tube. The nucleic acid detection tube is mainly added with EDTA + serum separation gel. It is suitable for the collection, transportation and storage of venous blood samples for nucleic acid detection. It is aimed at DNA amplification detection of HBV DNA, HCV and HIV. The serum separation gel can It can block the interference of hemoglobin in red blood cells on nucleic acid detection experiments   2.The PRP tube, the Chinese name "High Concentration Platelet Plasma", uses serum separation gel to extract platelet richness with precise specific gravity, and then injects it into the part we need, so as to achieve the effect of beauty repair and regeneration or treatment. So the PRP tube is not actually what to check, but to extract high-concentration platelet rich for reuse. 3. CPT tube, also called vacuum mononuclear cell preparation tube, uses different specific gravity of serum separation gel to separate lymphocytes and mononuclear cells from whole blood. It is used in clinical medical testing, mainly for HLA or residual leukemia gene testing, tuberculosis Testing, HIV testing, etc.   4. PST tube is mainly used to separate blood cells and serum, plasma, increase its output, ensure the stability of plasma composition, collect plasma samples, eliminate clotting time, and are mostly used for intensive care and emergency inspections.   Desheng is an established manufacturer of serum separation gel. It has invested heavily in machinery, equipment, production personnel, and R&D and quality inspection. After the continuous improvement of the first, second and third generations, the separation gel has been continuously improved. The separation gel developed and produced belongs to the fourth generation product. It has the advantages of more inert hydrophobic material and suitable for long-term storage of blood. Even if it is in contact with water for a long time, the pH value will not change. Moreover, Desheng's serum separation gel has also gained resistance. The patent of irradiated separation gel, the gold content of this patent is particularly high, welcome to consult and order!
Latest company new about Application of Carbomer in Bioadhesive Drug Delivery System
2020/09/19

Application of Carbomer in Bioadhesive Drug Delivery System

Carbomer, also known as Carbopol, is a high-molecular polymer cross-linked with acrylic acid and allyl sucrose ether or allyl pentaerythritol ether. Carbomer’s research began in the 1950s and was first developed and produced by Goodrich in the United States. By the 1970s and 1980s, the research on carbomer became mature and has been widely used, mainly in cosmetics and Pharmaceutical research and production. The following Desheng mainly explains the application of carbomer in bioadhesive drug delivery system. When carbomer is an anionic adhesive, it should not be used in combination with divalent basic drugs to avoid precipitation. The bioadhesive drug delivery system (BDDS) acts on various cavity epidermal cell mucosa or skin surface, such as gastrointestinal tract, vagina, oral cavity, nasal cavity, epidermis, etc. The dosage forms are tablets, membranes, and gels. Agent, stick, etc. Among them, gel is a new type of bioadhesive drug release system that has developed rapidly in recent years. It has good dispersion, strong adhesion, good stability, long-lasting drug effect, convenient application, and can avoid the first pass effect and the site of administration. Advantages of high concentration. (1) Gastrointestinal bioadhesive Sulpiride skeleton sustained-release retention tablets made of carbomer can adhere to the surface of gastrointestinal mucin or epithelial cells, prolong the retention time and achieve the purpose of sustained release. In vivo studies in rabbits have shown that compared with oral solutions and powder injections, the AUC of the sustained-release retention tablet can be increased by more than 1 time, the mean retention time (MRT) can be extended by 4 to 5 times, and the bioavailability is improved.   (2) Vaginal bioadhesive Carbomer 974NF was used to make metronidazole liposome and clotrimazole liposome into gel to treat vaginitis. In vitro experiments showed that after 24 hours of release of the two drug-containing liposome gels, approximately 50% metronidazole and 30% clotrimazole remained in the gels. And the stability test shows that Carbomer 974NF can maintain the particle size distribution of two liposomes, indicating that the bioadhesive liposome gel is suitable for local treatment of vaginal diseases. In order to avoid removal of the uterus in patients with uterine cancer, carbomer-based preparations can be administered through the vagina to allow the drug to adhere to the uterine mucosa, kill cancer cells without damaging normal cells, and avoid removing the uterus.   Using an appropriate ratio of hydroxypropyl cellulose (HPC) and carbomer as an adhesive, the bleomycin can be directly pressed into tablets or made into a duckbill suppository, which can be adhered to the cervix, and the preparation remains in its original shape after being taken out after 24 hours. It is speculated that this dosage form can achieve satisfactory results when used in the treatment of uterine cancer.   (3) Oral bioadhesive The drug can directly enter the systemic circulation after being absorbed by the oral mucosa, avoiding the first pass effect. The felodipine oral mucosal adhesive sheet prepared with hypromellose (HPMC) K4M and carbomer 974P as bioadhesive polymers has an apparent release rate constant of 3.3% h-1, and an average adhesive force of 131.08~181.35g. It has been verified by in vivo experiments that the preparation has a suitable adhesion force to the oral cavity and is less irritating to the oral mucosa. In order to obtain a good treatment of periodontitis, metronidazole oral adhesive tablets were prepared with carbomer 940 and hydroxyethyl cellulose (HEC). When the ratio of the two is 1:1, the drug loading of the product is 20mg. It can be released slowly in the oral cavity, and the drug concentration detected at 12h is higher than the minimum inhibitory concentration.   (4) Bioadhesive for nose Nasal administration can be aimed at special patients who are inconvenient or difficult to implement oral and intravenous administration. Carbomer 971P was used as an auxiliary material to develop apomorphine nasal powder mist. The sustained release study showed that the bioavailability of this dosage form is equivalent to that of subcutaneous injection, and it has sustained release effect. Najafabadi et al. reported that insulin was made into card Pom gel spray.   Carbomer can be divided into products of various specifications according to the degree of polymerization. The application of products of each specification will vary due to the degree of polymerization and viscosity. Among them, carbomer 934, 940, 941, etc. are the most widely used. . Desheng is one of the manufacturers of Carbomer, which can provide Carbomer 940 and 980, and you can call for consultation if you need it.
Latest company new about Comparing running buffer TAE and TBE
2020/09/18

Comparing running buffer TAE and TBE

We always add buffer solutions when performing DNA electrophoresis. Commonly used buffer solutions include TAE, which contains Tris, acetate and EDTA, and TBE (Tris-borate-EDTA).   What is the role of the buffer? One of the functions of the buffer is to maintain the pH of the solution stable. When an electric current passes through water, an oxidation reaction occurs at the anode to generate oxygen and hydrogen ions; a reduction reaction occurs at the cathode to generate hydrogen and hydroxide ions. Long-term electrophoresis will make the anode acid and the cathode alkaline. A good buffer system should have a strong buffering capacity to keep the pH of the solution at both poles basically stable. Another function of the electrophoresis buffer is to make the solution have a certain conductivity to facilitate the migration of DNA molecules. DNA is an alkaline substance that is negatively charged during electrophoresis (buffer pH=8) and migrates from the negative electrode to the positive electrode.   Another component of the electrophoresis buffer is EDTA, the purpose of which is to chelate Mg2+ plasma and prevent the activation of DNase during electrophoresis. Since nuclease needs these ions, EDTA can hold these ions firmly to avoid nucleic acid degradation. But it should be noted that magnesium ions are also cofactors of many enzymes, such as restriction enzymes, DNA polymerases, etc., so the concentration of EDTA is generally not too high (usually around 1mM). Desheng TRIS buffer packaging So which one is better, TAE or TBE? In fact, which one should I use for DNA electrophoresis? It really depends on the purpose of your experiment. Let's take a look at the difference between the two. 1. The conductivity of TBE is greater than that of TAE. Therefore, compared with TAE, TBE is less likely to cause overheating in the electrophoresis tank. TBE is recommended for long-term electrophoresis.   2. Boric acid is an enzyme inhibitor, so if you need to separate electrophoresis DNA for digestion reaction, it is recommended to use TAE as the electrophoresis buffer solution   3. TBE is better for separating smaller fragments. For fragments smaller than 300bp, the migration speed in TBE is faster on a 2% agarose gel.   4. TAE is suitable for the separation of large fragments. Fragments larger than 2kb migrate faster in TAE (in 0.8% agarose gel), and the speed is about 10% faster than in TBE. TAE is more suitable for recovery of DNA Fragment. Compared with TBE, TAE has higher resolution for supercoiled DNA.   In summary, the question of whether TBE or TAE is better cannot be generalized. The most suitable buffer system should be selected according to the specific experimental purpose. The biological buffer developed by Desheng has a wide buffer range, and only this kind of buffer is used. The system can prepare buffers with a wide range of pH values, and can provide various specifications of packaging. Buy buffers at Desheng Biochemical, welcome to consult and purchase.  
Latest company new about Acridine ester chemiluminescence quantitative immunoassay helps diagnose hepatitis B
2020/09/17

Acridine ester chemiluminescence quantitative immunoassay helps diagnose hepatitis B

This year’s World Hepatitis Day "Chao Wen Tian Xia" reported a series of astonishing figures: Hepatitis B and C affect 325 million people worldwide. At present, there are about 70 million hepatitis B virus carriers in my country, and about 20-30 million people need treatment. , And only 18% can be diagnosed. These data show that the prevention and treatment of hepatitis B is still a big challenge. Early screening and diagnosis is the key to effective control of hepatitis B infectious diseases. HBsAg is the first serum marker to appear after hepatitis B virus infection. It has high predictive value and is currently the most clinically used serum marker. It is also recognized by the WHO for judging HBV infection. Key indicators. High sensitivity, high specificity, and quantitative detection are the three major trends in HBsAg detection. Acridine ester chemiluminescence immunoassay has the characteristics of simple luminescence system, no catalyst, high sensitivity and few interference factors. It is widely used in the diagnosis of tumor markers, infectious diseases, especially viral hepatitis.   Some researchers have established a method for detecting HBsAg content in human serum/plasma based on the principle of chemiluminescence immunoquantitative analysis, using acridine ester labeling analysis technology and double-antibody sandwich method. This method uses a two-step method (washing twice). First, the sample is reacted with the biotinylated hepatitis B virus surface antibody (Anti-HBs). If the sample contains HBsAg, it will form an antigen-antibody complex , Adding streptavidin-coated particles, the complex forms a solid phase under the interaction of biotin and streptavidin. Then the reaction solution is placed in a magnetic field, the magnetic particles under test will be adsorbed, and the unbound substances will be washed and removed by washing. Then add the acridine ester labeled Anti. HBs reacts with the HBsAg complex on the magnetic particles, and the unbound substance is washed and removed by washing. Finally, a chemiluminescence buffer is injected to detect the intensity of the chemiluminescence photons, and the light intensity generated is proportional to the concentration of HBsAg in the sample.   The chemiluminescence quantitative detection kit based on the hepatitis B surface antigen acridinium ester chemiluminescence quantitative immunoassay method can be effectively used in the clinical diagnosis of hepatitis B and the dynamic monitoring of the disease. It has high sensitivity, good specificity, accurate quantification, and all performance indicators. It has the advantages of meeting clinical needs, and the price is relatively high in acceptability of imported reagents. It has great potential for large-scale clinical application and is of great significance for the prevention and treatment of hepatitis B.   Desheng is a professional company engaged in the research, development, production, sales and technical services of biochemical reagents, polymer materials and equipment, import and export of goods, technology import and export, and agent import and export. Although there is no ability to produce chemiluminescence quantitative detection kits, it can provide 6 kinds of acridinium ester products (acridinium ester DMAE-NHS, acridinium ester NSP-DMAE-NHS, acridinium salt NSP-SA, acridinium salt NSP-SA-NHS, acridine hydrazide NSP-SA-ADH, acridinium ester ME-DMAE-NHS), as well as the luminescent substrates luminol and isoluminol.
Latest company new about What inspection items can PCR technology be used for
2020/09/14

What inspection items can PCR technology be used for

Real-time fluorescent quantitative PCR technology is a leap forward in DNA quantitative technology. It is used to amplify specific DNA fragments, which can be regarded as special DNA replication in vitro. Through the DNA gene tracking system, the virus content in the patient's body can be quickly grasped with an accuracy of nanometer level. The real-time fluorescent quantitative PCR is the carrier to realize this technology.   The PCR laboratory is actually extremely powerful. Qualitative analysis and quantitative detection are its two biggest application directions. In addition to the new crown nucleic acid detection, what else can our "universal" PCR laboratory do? Next, I will show you a few examples of projects with specific application directions, and hope that these examples can provide medical systems at all levels with ideas and directions for project development. https://www.vacutaineradditives.com/products.html (1) Pathogen determination The advent of PCR technology enables rapid and convenient pathogen detection. Because the false positive rate of PCR technology is too high, a positive result can be obtained as long as there is a small amount of pathogens, which cannot be used as a diagnostic basis, and it has clinical significance only when a certain number of pathogens exist. Therefore, it is particularly important to accurately quantify the template, and the results can be obtained quickly and accurately by using fluorescent technology PCR. PCR can be used to solve the "window period" of immunological testing, determine whether the disease is in a recessive or subclinical state, and when antibody testing cannot determine whether it is a current infection or a past infection.   (2) Genetic disease detection Gene mutation and copy number variation are the main genetic basis of genetic disease and the main target of genetic disease detection. The complexity of genetic diseases is accompanied by a large number and wide types of gene mutations; gene copy number variation is not only manifested as deletions and duplications, but also the position, size and replication multiples of the deletions are diverse. The complexity of genetic variation poses a technical challenge for the clinical detection of genetic diseases. Real-time PCR technology is a new generation of real-time PCR technology that we have recently developed. Using fluorescent labeling or melting point analysis, multiple targets can be detected in a single reaction tube.   (3) Personalized medication The development of pharmacology and pharmacogenomics has clarified the genetic nature of individual differences in drug metabolism and effects. Abnormal drug reactions are mainly caused by mutations in drug metabolizing enzyme genes that lead to abnormal enzyme activity, which in turn leads to the failure of normal metabolism of drugs in the body after taking the drug. Elimination and excretion from the body make the drug concentration in the body too high or too low, and the ideal therapeutic effect cannot be achieved. This kind of abnormal drug reaction can be used to detect genetic genes related to the drugs taken by the patient, adjust the drug dosage or search for alternative drugs, to maximize the formulation of a more reasonable, effective and economical drug treatment plan.
Latest company new about What are the blood test items, and what are the corresponding blood collection tubes and reagents?
2020/09/11

What are the blood test items, and what are the corresponding blood collection tubes and reagents?

Blood testing is very important, it involves many items. Vacuum blood collection tube additives are used in the sampling process of blood testing, and their role is to maintain the original properties of the blood to ensure high-quality blood samples. Here is a brief introduction to the specific items of blood testing.   Blood biochemical test: 1. Liver function test, ALT, AST, GGT, ALP, TBil, DBILI, IBILI, TP, ALB, BIB, A/G, ADA, PAB, AFU, CHE, liver function+, TBA, LDH, CH, GPDA, NUT, MAO, GLDH, ASTm, protein electrophoresis, etc. The blood collection tube uses a red cap tube to self-coagulate or use a coagulant tube containing a coagulant.   2. Renal function test, EURA, CREA, U/C, UA, β2-MG, CC, etc. Use self-coagulating blood vessels or coagulant tubes for blood collection.   3. Blood lipid testing, TG, Chol, APOA1, HDL-C, LP(a), etc. Self-coagulating blood vessels or coagulant tubes are used for blood collection. Blood test 4. Myocardial enzyme detection, AST, CK, CK-MB, LDH, etc., use self-coagulating blood vessels or coagulant tubes.   5. Electrolyte detection, potassium, sodium, chlorine, calcium plasma, CO2, AG, etc., use self-coagulating blood vessel or coagulant tube.   6. Blood glucose and glucose tolerance test, blood sampling with grey cap anticoagulant tube.   7. Detection of glycosylated hemoglobin and hepatitis B indicators, using a purple cap anticoagulant tube.   Blood immune test: 1. Full tumor detection, tumor markers, gastrointestinal tumors, digestive tract tumors, lung cancer series, etc. Self-coagulating blood vessels or coagulant tubes are used for blood collection. 4 mL of blood is collected for all tumor items, 3 mL for other combined items, and 2 mL for single items.   2. Humoral immunity, IgG, IGA, IgM, C3, C4, etc., self-coagulating blood vessels or coagulant tubes for blood collection.   3. Rheumatism indicators, inflammation indicators, autoimmune liver antibody spectrum, autoantibody series, use self-coagulating blood vessels or coagulant tubes.   Professional blood test: 1. Blood cell analysis, reticulocyte count, purple cap tube, anticoagulant EDTA dipotassium tube.   2. Determination of red blood cell sedimentation rate, using black cap tube, 3.8% sodium citrate as anticoagulant, adding ratio 1:4 to blood collection volume.   3. Four items of coagulation, plasma prothrombin time, plasma fibrinogen, activated partial thromboplastin time and plasma thrombin time, using light blue cap blood collection tube, anticoagulant with 3.2% sodium citrate, added amount The ratio of blood sampling is 1:9.   4. Red blood cell fragility test, hematocrit test, blood gas analysis, use a tube covered with a green head, and the anticoagulant is heparin. Electrolyte detection uses lithium heparin as an anticoagulant.   The above are the commonly used test items related to blood, as well as blood collection tubes and additives for blood sampling. Desheng has fifteen years of experience in the field of blood collection reagents. The additives produced include serum separation gel, blood coagulant, blood anticoagulant EDTA salt, heparin, sodium citrate, etc.
Latest company new about Application of EDTA-2K anticoagulant in erythrocyte sedimentation rate detection
2020/09/10

Application of EDTA-2K anticoagulant in erythrocyte sedimentation rate detection

ESR (erythrocyte sedimentation rate ESR) refers to the sinking speed of red blood cells under certain conditions. ESR can increase significantly in many pathological conditions. The pathological reasons leading to the marked increase in erythrocyte sedimentation rate are mainly found in various inflammations, tissue damage and necrosis, malignant tumors, hyperglobulinemia and anemia. ESR is often used clinically to observe the activity and dynamic changes of tuberculosis and rheumatic fever.   Because most of them need to test the blood routine at the same time, the blood collection volume is relatively large, especially for children. The author uses EDTA-2K+ anticoagulant to determine and evaluate the erythrocyte sedimentation rate, and make related experiments. The experimental method is: take about 3.2 mL of the subject's venous blood, take 1.2 mL and put it into an erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant, gently invert and mix; then take 2.0 mL of venous blood The human blood test tube containing 30ul 15% EDTA-2K anticoagulant is gently inverted and mixed, and then 1.2 mL of it is added to the erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant. The tube also measures the erythrocyte sedimentation rate. Blood collection tube additive EDTA-K2 The principle of anticoagulation between EDTA and sodium citrate is to form a chelate with calcium ions in the blood to prevent blood from clotting. However, the anticoagulant effect of EDTA is stronger than that of sodium citrate, and the two do not interact with each other. For interference, the principle of using the automatic erythrocyte sedimentation rate analyzer is dynamic erythrocyte sedimentation rate analysis. Sodium citrate anticoagulant blood is added to a special test tube, and the fixed hole inside the instrument is vertically placed. According to the setting, the photoelectric detection part of the instrument will scan each cuvette up and down regularly. According to the different light transmittance of glass tube plasma and whole blood, the instrument automatically records the plasma height each time. Through computer processing, the instrument can report the erythrocyte sedimentation rate of 60 minutes and draw the erythrocyte sedimentation time curve.   The experimental results show that the ratio of anticoagulant to whole blood has a great influence on the erythrocyte sedimentation rate, and a high anticoagulant ratio will cause a low erythrocyte sedimentation rate. The test tube used in the instrument method has a small diameter. In actual work, it is common that the amount of blood collected from the sample is not accurate; and the EDTA-2K+ anticoagulant blood measured by a blood cell analyzer is used for the erythrocyte sedimentation rate determination, and the same charge is maintained by complementing the anticoagulation ratio , To ensure the accuracy of the results. This article proves through experiments that EDTA-2K+anticoagulant blood can be used for instrumental ESR determination, and the results are accurate and reliable.   Desheng specializes in the research and development, production and sales of blood collection tube additives and blood test reagents. In terms of blood collection tube additives, it has formed independent intellectual property rights and professional production research and development capabilities. We have accumulated a wealth of knowledge and experience in the pretreatment of blood samples and have the advantages of professional technical services. Welcome to consult and purchase.
Latest company new about Timely delivery of specimens is so important for biochemical testing
2020/09/09

Timely delivery of specimens is so important for biochemical testing

The quality control of clinical samples before analysis and testing is extremely important, but in actual work, inspectors often ignore the impact of specimen placement time on the accuracy of the test results, resulting in many specimens that cannot be sent for inspection soon after collection. The process of specimen transportation and the separate inspection of different batches caused a lot of time delay for some specimens. In addition, many hospitals did not indicate the collection time of the specimen on the specimen submission form, and the specific placement time of the specimen is not known. As an inspector, you must have a certain understanding of the impact of specimens placed for too long on the results of different items.   https://www.vacutaineradditives.com/products.html   Impact on biochemical test results 1. Blood sugar: GLU of blood samples at room temperature degrades by about 5% per hour, mainly due to the glycolysis of glucose and the consumption and absorption of sugar by cells. After the blood is separated, glycolysis provides energy for red blood cells, bacteria decompose glucose, and leukocyte degrading enzymes. These factors will cause the blood glucose concentration to gradually decrease with the prolonged storage time.   2. Electrolytes: With the prolongation of the time of blood isolation, the ATP production barrier and the increase of cell membrane permeability lead to the transfer of ions inside and outside the cell. K+ and Na+ levels increase after 4 hours, and to a greater extent after 8 hours. Fluctuations.   3. Liver function indicators: 4 hours after the blood sample was placed, AST and ALP increased, while TBIL and ALT decreased. The main reason is that AST and other factors in red blood cells can be transferred to the outside of the cell to increase the enzyme activity and accelerate the lysis of red blood cells, while TBIL can be oxidized with the bilirubin, resulting in abnormal cytokine levels.   4. Kidney function indicators: blood Scr is not affected by the time of specimen placement, while blood BUN and β2-microglobulin are significantly reduced. It may be that the lipoprotein particle structure has changed or even cracked during the placement of lipoprotein cholesterol, so BUN The levels of β2-microglobulin and β2-microglobulin decreased as the time of blood isolation increased.   It turns out that timely delivery of specimens is so important for biochemical testing. Therefore, in order to control the quality of the test, it is necessary to complete the test of biochemical indicators within 4 hours of placing the blood specimen to ensure the accuracy of the results. Desheng produces blood collection tube additives. The old brand manufacturers, including blood anticoagulant, coagulant, serum separation gel and other products, can be applied to various biochemical testing projects. It is derived from the trust in product quality and professional services. It has accumulated a lot of long-term Repurchase customers, Desheng is your trusted choice!
Latest company new about These diseases need to be prevented in the beginning of the school season in September
2020/09/08

These diseases need to be prevented in the beginning of the school season in September

When registering in September, students have stepped into the school gate, and the lively September has already arrived. This year's students have spent the longest winter vacation in history due to the impact of the epidemic, so they cannot relax their vigilance when the school starts. Desheng comprehensively analyzes the epidemic data of infectious diseases over the years, domestic and foreign epidemic trends, and meteorological and hydrological data, and hereby remind everyone that in September, we should pay special attention to new coronary pneumonia, dengue fever, hand, foot and mouth disease, and foodborne infectious diseases! And sorted out some preventive measures for these diseases. https://www.vacutaineradditives.com/products.html 1. New coronary pneumonia High-risk places: places where people gather at homes, schools, and workplaces. Transmission route: mainly spread through respiratory droplets, close contact, and indirect spread through contact with contaminated items. Symptoms: The main manifestations are fever, dry cough, and fatigue.   Precaution: (1) After the school starts, the school strictly implements daily management, and grasps the health status of faculty, staff and students daily. (2) Carry out the registration system for entering and leaving the school, keep the school doors closed; strengthen indoor ventilation, keep the classroom clean, and high-frequency contact with the surface of objects in public areas, such as door handles, desks and chairs, lecterns, stair rails, elevator buttons And so on, arrange for someone to clean and disinfect every day. If air conditioning is used, the air supply safety of the air conditioning system should be ensured. (3) Keep a safe distance from others in the dining line. No outsiders are allowed to enter the dormitory. Students do not gather or visit in the dormitory area. (4) Masks must be worn when in close contact with people, in crowded, closed places, or in transportation. (5) When there are suspected symptoms such as fever and dry cough, take the initiative to go to a regular hospital for treatment. During the treatment process, you must wear a mask and avoid taking public transportation.   2. Dengue fever Places with high incidence: homes, construction sites, schools and other places where people gather and where there are many mosquitoes. Transmission route: Aedes spread. Symptoms: persistent fever, headache, muscle aches, rash, flushing of face, neck, chest, etc. Preventive measures: The key to dengue fever is to prevent mosquitoes. In recent times, there have been continuous rains and humid air in many places. Friends of the public should always check all kinds of stagnant water in their homes that may have mosquitoes, clean up dead corners, and turn over pots. When going out, wear long-sleeved trousers or apply repellent, and use mosquito coils and mosquito nets at home to prevent mosquito bites.   3. Hand, Foot and Mouth Disease High-incidence places: kindergartens. Transmission route: Respiratory tract transmission, contact with children, and indirect contact with dirty hands, toys and utensils. Symptoms: fever, maculopapular rash and herpes on the palms, soles, buttocks, and herpes or ulcers on the oral mucosa.   Precaution: (1) Pay attention to household and surrounding environmental hygiene, and pay attention to personal hygiene. Wash your hands frequently, do not drink raw water, and do not eat raw or cold food; caregivers should wash their hands before touching children, changing diapers for young children, or after handling feces; often ventilate the room and dry clothes and quilts frequently. (2) The condition of hand, foot and mouth disease in young children changes rapidly, so they should not deal with it by themselves to avoid delay in treatment. Pay close attention to changes in children's conditions and seek medical attention in time. (3) At present, there is no specific medicine for hand, foot and mouth disease. Children of the appropriate age can voluntarily vaccinate hand, foot and mouth disease at their own expense. The EV71 vaccine is targeted for susceptible children ≥ 6 months of age. The sooner the vaccination is, the better; the vaccination procedure is encouraged to be completed before 12 months , In order to play a protective role as soon as possible.   4. Foodborne diseases High-incidence places: unit canteens, school canteens and other collective dining places Transmission route: food transmission. Popular throughout the year, with high incidence in summer and autumn. Symptoms: Mainly acute gastrointestinal symptoms, such as nausea, vomiting, abdominal pain, diarrhea, and occasionally accompanied by fever.   Precaution: (1) Ensure food safety and wash hands frequently. Choose fresh foods, do not buy foods of unknown origin and abnormal sensory properties, and do not eat foods that have exceeded the shelf life. (2) The key is to separate raw and cooked, and cook thoroughly. Generally speaking, fresh food must be fully heated before eating, and seafood and meat must be cooked thoroughly to ensure food safety. (3) When dining out, you should choose a restaurant with a catering service license, and not eat at restaurants with poor sanitation and mobile vendors.
Latest company new about Introduction to enzyme preparation and its classification method
2020/09/07

Introduction to enzyme preparation and its classification method

Enzymes come from nature and are natural catalysts for thousands of biochemical reactions that are carried out quickly and efficiently in the body all the time. The natural sources of enzymes include animals, plants and microorganisms. At present, the application of enzyme preparations in detection has become increasingly widespread. Today, Desheng will lead you to understand what is an enzyme preparation and what are its classification methods?   What is an enzyme preparation? Enzyme preparations refer to a class of substances with enzyme characteristics extracted from organisms. Enzyme preparations are proteins composed of amino acids produced by living organisms and can control many reaction processes and biological activities of plants, animals, humans and microorganisms. It is highly efficient, specific, and active under suitable conditions (pH and temperature). Classification method of enzyme preparation: 1. Classification by source (1) Animal's own enzymes: produced and secreted by various secretory glands of animals, almost including all kinds of enzymes required by animals. (2) Artificial enzymes: The artificially extracted enzymes are mainly digestive enzymes.   2. Classified by mechanism of action (1) Hydrolysis enzyme Such enzymes mainly include amylase, protease, lipase, cellulase, phytase, pectinase and so on. (2) Oxidoreductase Oxidoreductase refers to enzymes that participate in the redox of organic substances. There are mainly dehydrogenases and cytochrome oxidases, which are found in the body fluids and tissues of animals and plants, and are not used much in feed additives.   3. Classification according to whether animals can be synthesized (1) Digestive enzymes: livestock and poultry can synthesize these enzymes and digest nutrients, but they need to be strengthened and supplemented for some reason. Such enzymes mainly include amylase, protease and lipase. (2) Non-digestive enzymes: most of the enzymes that animals cannot secrete into the digestive tract are derived from microorganisms. Such enzymes can digest substances that the animals cannot digest or degrade some anti-nutritional factors.   4 Classification by preparation type (1) Single enzyme preparations: such as amylase, lipase, protease, cellulase and phytase. (2) Compound enzyme preparation: It is composed of one or several single enzyme preparations as the main body and mixed with other single enzyme preparations, or obtained by fermentation of one or several microorganisms. The compound enzyme can simultaneously degrade a variety of substrates that need to be degraded in the diet, that is, a variety of anti-nutritional factors and a variety of nutrients, which can maximize the nutritional value of the feed. Feed enzyme preparation products at home and abroad are mainly compound enzyme preparations.   Enzyme preparation is an indispensable additive in our human production and life, and it has a great role in promoting human development. The enzyme preparation products provided by Desheng are mainly used as the test materials of the kit, including α-glucosidase, cholesterol esterase, uricase, coenzyme A, cholesterol oxidase, purine nucleoside phosphorylase, glucose oxidase, etc.
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