China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Forensic Medicine   Luminol is commonly used in forensic medicine as a diagnostic tool for detecting blood stains. Most crime scene investigations (called criminology) are based on the fact that no traces will not disappear, and small blood particles will stick to most surfaces for many years. Luminol reveals these traces through luminescent chemical reactions between several chemicals and hemoglobin (an oxygen-carrying protein in the blood).   Nucleic acid determination   The luminescent oxygen channel immunoassay (LOCI) type detection method is also suitable for the detection of single nucleotide polymorphism types. Other detection methods based on luminescent nucleic acids include the detection of infectious diseases combined with nucleic acid amplification technology, such as polymerase chain reaction, such as telomere DNA, herpes simplex, Lassa fever virus and Trichomonas vaginalis.   Oncology   HRP molecules catalyze the luminescence reaction between luminol and H2O2 to produce an enhanced luminescence signal. Under optimal conditions, the luminescence intensity depends on the surface coverage of HRP molecules (related to the concentration of p53 protein), and linearly increases with the concentration of p53 protein between 0.01-0.5 nM. The estimated detection limit is 3.8 pM. This assay is a successful method for detecting p53 protein in normal and cancer cell lysates.   DNA Testing   Another successful research application of CL is the detection of gene expression products developed as a substitute for the traditional chloram phenicol acetyltransferase gene. Chemiluminescent dioxetane and luminol derivative substrates can also be used to detect and quantify the gene expression products of placental alkaline phosphatase, β-galactosidase and β-glucuronidase. These measurements are sensitive and have a linear range on several orders of magnitude.   Cell chemiluminescence   The existing cell location methods are not enough. Therefore, the enhanced luminescence of luminol or luminol emitted by polymorphonuclear neutrophils or phagocytes or biological fluids (such as whole blood) is an important tool for cell-based research (including respiratory burst research).   Protein quantification   In routine laboratory research experiments, Western blotting is used to quantify proteins. Western blotting is widely used to study steady-state protein levels, biosynthesis and turnover rates, the effects of mutations on protein stability, and the effects of pharmacological compounds on protein expression.   Environmental monitoring   Luminol can be used to determine the content of metal ions. A method for determining the content of iron ions in seawater has been established and used for marine environmental monitoring.   medicine analysis   The myoglobin-luminol system is used to detect the drug Aniracetam, which is used for the treatment of the central nervous system. The myoglobin conjugate complex of Anracetam catalyzes the CL reaction of luminol, which is enhanced compared with the CL reaction without Anracetam. This method is also used to determine cefazolin sodium in injections and human urine.

Biological buffers are the most commonly used auxiliary reagents in biochemical testing, and buffers are required for almost all liquid phase reaction systems. Its main function is to maintain the pH value of the reaction system. Commonly used buffers include phosphate, acetate, ammonium salt, and Tris base, MOPS, HEPES, etc. in biochemical experiments.   There are many effects of biological buffers on biochemical detection, mainly in the pH value of the solution. The specific effects are illustrated below. A variety of biological buffers   The effect of buffer on enzyme activity:   Most biochemical tests are reactions involving enzymes. The catalytic efficiency of enzymes is greatly affected by pH and temperature. Each enzyme has its optimal pH value (the pH value at which the enzyme catalytic reaction efficiency is maximum) Therefore, biochemical detection involving enzymes needs to first select a buffer with a suitable buffer range according to the optimum pH value of the enzyme preparation used. Such as Trinder’s reagent enzymatic detection, enzyme-linked immunoassay, etc.   The effect of buffer on the structure of macromolecules:   In addition to the effect of the buffer on the catalytic efficiency of the enzyme, it may also affect the structure of the enzyme. If the pH value of the system and the adaptation pH value of the enzyme are too different, the enzyme may be directly inactivated. On the other hand, even if there is no reaction involving enzymes, buffers are necessary. Many macromolecular substances are often involved in biochemical detection, and their structure is also affected by pH.   For example, acridine ester chemiluminescence detection. Although the reaction of acridine ester and hydrogen peroxide does not require enzyme catalysis, it can emit light directly, but in CLIA analysis, acridinium ester is usually labeled protein, antigen, antibody or nucleic acid, these macromolecular substances The structure is complex and is affected by the pH of the system, so biological buffers are also required.   The effect of buffer on osmotic pressure:   Many buffers are organic weak acid and weak base conjugate acid-base pairs. Different buffers have different ionic strengths, which affect the salt concentration and osmotic pressure of the system. HEPES is often used as a buffer for cell culture, and a buffer that affects the osmotic pressure of the cell membrane and may cause rupture cannot be used.   In addition, different buffers have different complexing capabilities for different metal ions. For example, the system containing calcium and magnesium ions uses Tris buffer instead of PBS. The activity of many enzymes and proteins is related to the concentration of metal ions. Desheng is an established manufacturer of biochemical buffers, which can supply more than ten kinds of biological buffers.

Desheng is a professional manufacturer of TRIS (tromethamine) buffer. Our products are sold all over the country and are widely used in the field of biotechnology. Desheng has more than ten years of experience in the production of TRIS. In the future, we will continue to provide TRIS products that meet the needs of customers for the booming global market to ensure excellent functionality and compliance. For traditional TRIS buffer products, product agglomeration is a long-standing headache and huge challenge in the industry. There are many inefficiencies caused by TRIS agglomeration, such as increased labor costs and operating costs to handle agglomeration; another example is to process buffer solutions, which prolongs the time required for mixing, reduces production efficiency, and forms an operating bottleneck . Magnified image of Desheng TRIS crystal Causes of TRIS agglomeration TRIS is a hygroscopic material that will absorb moisture. When the environment and/or storage conditions of TRIS are not ideal, such as being affected by humidity, pressure, temperature and other factors, and lasting for a period of time, it will cause different degrees of agglomeration. The common lumps are loose and large particles in the powder, but they may also form solid lumps. Affected by the transportation time and conditions, the traditional TRIS on the market is often accompanied by agglomeration when it arrives, and in the future storage or daily distribution operations, if the package is re-sealed after the package is opened, there will be a secondary knot. The block phenomenon occurs. Desheng TRIS advantage In order to meet the processing challenges of TRIS buffers on the current market, Desheng Technology produces through proprietary process technology, which is not easy to agglomerate and harden-and does not change the structure of the molecule itself, providing a solution to the problem of agglomeration . Desheng buffer has a larger and rounder particle structure, and it is not easy to agglomerate even under various packaging, transportation and storage conditions. In terms of processing and preparation, key operational benefits can be generated: Desheng TRIS buffer features 1. The adhesion of particles between crystals is low, and the processing of agglomeration is more labor-saving 2. Smoother particle structure makes it easier for the feeding system to disperse and transmit 3. Reduce the number of fine particles, thereby reducing dust emissions 4. No additional additives or anti-coagulants added 5. Less clumping As a professional TRIS (tromethamine) manufacturer, Desheng always insists on providing high-quality products and services. Our fully traceable raw materials, reliable and stable global supply, professional testing and customized packaging solutions provide you with strong support in the biological field-from research and development, application development to production, which is not only our consistent Commitment is our persistence from beginning to end.

Tris base is a weak base with a wide range of uses. It can be used in biological buffers, biopharmaceuticals, coatings, new composite materials, etc. Sodium carbonate is the reference substance for titration calibration of acid concentration, but it has some shortcomings, and Tris can be used as a supplement to sodium carbonate reference substance to replace it for titration calibration of acid concentration. Tris(hydroxymethyl)aminomethane powder Experiment preparation and equipment: Hydrochloric acid: analytically pure; sulfuric acid: analytically pure; sodium carbonate: reference reagent; Tris: reference reagent; methyl red-cresol green ethanol solution. Potentiometric titrator, Lab X workstation, composite pH electrode; muffle furnace; 50mL acid burette and other general laboratory utensils. Use Tris and sodium carbonate to titrate hydrochloric acid or sulfuric acid separately: Manual titration: Weigh the standard reagent Tris (constant weight at 110 degrees Celsius) or anhydrous sodium carbonate (constant weight at 270-300 degrees Celsius) which consumes about 20-30 mL of the sulfuric acid or hydrochloric acid titration solution to be calibrated, weigh to 0.0001g, and dissolve in Add 10 drops of bromocresol green-methyl red indicator to a 250mL Erlenmeyer flask containing 50~100mL of water, and change the acid solution to be calibrated from green to dark red. Boil for 2min. After cooling, continue to titrate until the solution just changes color ( Light gray). Do a blank test for the acid standard titration solution of not more than 0.1mol/L at the same time. Automatic potentiometric titration: Fix the titration cup containing the reference anhydrous sodium carbonate or Tris aqueous solution on the titration stand, connect the PH composite electrode, set the appropriate titration parameters of the automatic potentiometer, and start the titration program to automatically titrate to the end point. (It is appropriate to consume sulfuric acid or hydrochloric acid titrant 2. ~ 30mL). At the same time, make a blank test. Compared with manual titration, automatic potentiometric titration has more advantages. Manual titration adopts calibration indicator. The selection is based on the sudden change in the pH value of the solution near the stoichiometric point of the titrant. The end point is judged by the color change of the indicator, and the color range is affected by different concentrations. The acid content and the quality of the reference material vary. Usually, the indicator method must be used to calibrate the end point color by the potentiometric method. Potentiometric titration is based on potential mutation sites. The potential mutation sites of Tris are more prominent and single, while sodium carbonate has multiple interference mutation sites. Tris potential titration has more advantages in calibrating acid concentration. Desheng has a large-scale Tris production line, and its products are stable and guaranteed.

In the related biochemical testing of enzyme preparations, in addition to measuring the content, concentration, and stability of various indicators to be tested, there are also indicators for detecting enzyme activity, such as blood sugar, blood lipids, and creatinine. For indicators such as transaminase and sarcosine oxidase, enzyme activity needs to be measured.   Generally speaking, enzyme activity is the activity of enzyme catalysis, which involves a kinetic parameter of the enzyme-the Michaelis constant Km, which means the concentration of the substrate (S) when the enzymatic reaction reaches half of the maximum speed (Vm) . The speed of the enzyme reaction is not uniform, not linear like the concentration, but the number of meters in Changshu is constant, which is a characteristic physical quantity of the enzyme. The Michaelis constant of the enzyme changes with the measured substrate type, reaction temperature, pH and ionic strength. Under the same conditions, no matter what the concentration of the enzyme is, the concentration of the required substrate is the same when the maximum reaction rate is reached.   Measurement method of Michaelis constant of sarcosine oxidase: Prepare a sarcosine solution with a concentration of 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0mmo1/L with Tris-HC1 buffer of pH 8.0, and react at 37°C for 5 minutes Determine the initial oxidation activity of SOX at different sarcosine concentrations. The reaction rate V and 1/V are obtained. According to the Lineweaver-Burk double reciprocal mapping method, with 1/[s] as the abscissa and 1/V as the ordinate, the Km and Vmax of SOX to sarcosine were calculated.   Conclusion of Michaelis constant of sarcosine oxidase: The Michaelis constant can be used to judge the specificity of the enzyme. The smaller the Km value, the greater the affinity between the enzyme and the substrate, and the more suitable the substrate is as the natural substrate of the enzyme. According to the test and calculation of sarcosine oxidase above, the Michaelis equation is y=1232.5X+8.7012 and the correlation coefficient is 0.9947: the calculated Km and Vmax values ​​of SOX to sarcosine are 141.6mmol/L and 0.115mmol, respectively /(L.min).   It should be noted that sarcosine oxidase SOX from different sources has a certain difference in the Michaelis constant of sarcosine. For example, the Km value of SOX from Corynebaclerium to sarcosine is 21mmol/L. Since the Michaelis constant has nothing to do with the concentration of the enzyme and the type of enzyme, this point can also be used for enzyme identification or determination. Desheng Biochemical focuses on the field of biochemical detection, and provides a variety of enzyme preparations including SOX, cholesterol esterase, glucose oxidase, etc.

It rained and dredged the wind last night. I worked overtime to improve the steps. I asked the researcher, but the result was the same. "Do you know? Do you know? It only makes people lose weight." In the face of heavy and arduous R&D tasks, how to choose the right enzyme preparation? How to maximize the effectiveness of raw materials? These problems have plagued countless people. If you learn to read the instructions of enzyme preparations correctly, perhaps these problems will be solved. 1. Understand the color of enzyme powder In vitro diagnostic reagents will be marked with the color of the liquid reagent when they are registered. The color of enzyme preparations will affect the color of reagents after feeding; for example, COX, FPOX, SOX, etc. are all light yellow or yellow powders, and most of the reagents prepared are also light yellow or yellow. It is important to choose an enzyme preparation with an appearance color similar to the registration information. It is not only necessary to carefully check the packaging before feeding, but also to check whether the appearance of the enzyme preparation powder is consistent with the description in the instructions. 2. Understand the specific activity parameters of enzyme preparations When calculating the feeding amount, the specific activity is a parameter that must be understood, and the feeding amount can be determined after conversion. In addition, specific activity is also an important parameter for comparing enzyme preparations. The enzyme preparation itself is a protein powder, which can be used as a nutrient for the growth of microorganisms, which will introduce pollution. Choosing enzyme preparations with high specific activity can reduce the amount of feed, reduce the problem of microbial contamination, increase the shelf life of diagnostic reagent products, and improve product quality. 3. Understand the pH range of enzyme preparations People have their own comfort zone. They don't like severe cold or intense heat. They like to breathe freely in the fresh air. The same is true for enzyme preparations. Each enzyme preparation has its own optimum range. Only at a certain pH and temperature can the enzyme preparation be stimulated to maximize its catalytic activity. Find the optimum pH value from the instructions, and then set the pH value of the reagent within this range, so that the enzyme molecule can work in the best state. 4.understand the interfered substances of enzyme preparations The activity of enzyme preparations will also be interfered by the required substances, such as metal ions such as Ag+, Hg2+, Fe3+, etc. The instructions will also list common interfering substances, which is convenient for production personnel to avoid the introduction of such substances during the development and production of reagents. Enzyme activity. The above important information can be found in the "Enzyme Preparation Manual". As a professional manufacturer of enzyme preparations, Desheng Technology can not only provide enzyme preparation raw materials comparable to imported brands, but also has diagnostic reagent development experience and can provide Mature diagnostic reagent products.

Tris is also called tromethamine, cas77-86-1. Trihydroxymethyl aminomethane (Tris base) is often used as biological buffer, TAE and tbe buffer (used for nucleic acid dissolution) are commonly used in biochemical experiments. What other applications does it have? Now let's take a look.   In the field of medicine, Tris is not only an important pharmaceutical intermediate, but also a drug itself. It is suitable for metabolic acidemia, respiratory acidemia and other diseases, especially in the treatment of hyperuricemia, with ideal effect.   Hyperuricemia can be divided into primary hyperuricemia and secondary hyperuricemia, mainly due to excessive source or (and) insufficient excretion of uric acid. Under normal circumstances, if the level of uric acid in men is higher than 420 μ mol / L and that in women is higher than 360 μ mol / L, it is hyperuricemia. According to social understanding, the high incidence age of hyperuricemia is middle-aged and elderly men and postmenopausal women, and there is a trend of younger in recent years.   At present, the prevention and treatment strategies of hyperuricemia mainly include reducing intake, inhibiting endogenous synthesis, and promoting renal excretion. Among them, promoting renal excretion is mainly achieved by alkalizing urine and inhibiting uric acid transporter. Inorganic metal cations such as Ca2 +, Mg2 +, NH4 + in urine can inhibit the dissolution and excretion of uric acid, while alkaline factors can promote the dissolution of uric acid. Therefore, trimethylolmethane and sodium bicarbonate are often used in this process. However, trimethylolmethane is better than sodium bicarbonate in promoting the dissolution and excretion of uric acid.   After taking sodium bicarbonate, the increased cationic Na + will further antagonize HCO3 alkalization of urine and blood, and promote the dissolution and excretion of uric acid. When sodium bicarbonate exceeds a certain dose, it will even reduce the solubility of uric acid. However, sodium bicarbonate can be produced endogenous. There is sodium bicarbonate in the human body, and there is a great fluctuation in the urine, so the concentration of sodium bicarbonate is not easy to control. If you take a small amount of sodium bicarbonate, the effect of excreting uric acid is not good. If you take more sodium bicarbonate, it may aggravate the deposition of uric acid in the urinary system, and the intake of sodium salt will also increase the cardiovascular and renal burden.   Tris is an organic amino base without inorganic cations, which can avoid or weaken the inhibition of inorganic cations. In addition, Tris is a foreign substance, so the body will not produce Tris. Therefore, the concentration of Tris in the body is better controlled than that of sodium bicarbonate in the course of medication The dissolution of uric acid promoted by trimethylaminomethane was better than that by sodium bicarbonate.   Desheng has been making deep research on biological buffers, and can provide high-quality raw materials such as Tris, bicine, HEPES, etc. Desheng adheres to the company's philosophy of "quality first, technology leading" to provide customers with high-quality products.

Introduction:   Carbomer 940, CAS 9003-01-4, also known as carbomer 940, is a kind of high molecular polymer cross-linked by acrylic acid and acryloyl sucrose or acryloyl pentaerythritol. It is a white loose powder with characteristic slight odor and strong hygroscopicity. Its average water content is 8%. Carbomer resin exists in water in the form of acid. It is easy to swell in water and polar organic solvents (such as ethanol, glycerin, etc.) Characteristics of colloidal solution. Because of 56% - 68% carboxylic acid group in the molecule, these resins are weakly acidic. Although they are weaker than acetic acid, they can easily react with inorganic bases and organic bases to form salts.   Because of its swelling and weak acidity, it is a very important rheologic regulator. The neutralized Kabo resin is an excellent gel matrix with thickening, suspension and other important properties. It has high transparency, high viscosity, strong suspension ability, very short rheology and thixotropy, low ionic shear resistance, simple process and good stability. It is widely used in shampoo, shower gel and bath. Cream, cream and other cream products are especially suitable for transparent products. The most important point is that carbomer 940 is mild and not irritant to skin. Carbomer 940 powder matters needing attention:   1. PH value: the best pH value range of Carbopol 940 is 4-10, higher or lower than this range will lead to changes in the viscosity of the system.   2. The components in the formula that are not easy to be compatible: protein, PVP resin, polyethylene glycol (PEG), polyethoxylated surfactant will interact with the non neutralized Carbopol 940, and it is necessary to partially neutralize Carbopol 940 before adding.   3. The presence of electrolyte will reduce the thickening efficiency of Carbopol resin: Carbopol 940 is sensitive to salt and cation, when the concentration of soluble ion in the component is greater than 0.1%, the dosage of the component should be reduced appropriately.   Deionized water can be used as the matrix of the main material, and salt can be added after neutralization to master the influence of salt on the system. High valence ions (CA, Mg, Fe, AI) will cause serious damage to the system and should be removed. The Fe and Cu converted in the production process will reduce the viscosity of the system and lead to the instability of the system. Stainless steel or non-metallic equipment can be used to prepare products to avoid such effects. In addition, adding EDTA to chelate metal ions can also achieve good results. In addition to the above methods, the appropriate model of Carbopol 940 (such as Carbopol 940l342ge resin) can also be selected, which has low sensitivity to soluble salts.   4, insoluble matter: when there are insoluble components, carbo 940 system is difficult to present clear and transparent gel, and solubilization technology can reduce or eliminate this effect.   5. High shear agitation and pump delivery: carbo 940 is thickened by forming a gel skeleton. After Carbopol neutralization, high shear stirring, long-term stirring, grinding or pump chipping will damage its skeleton and cause viscosity loss, so pipeline homogenizer should be used. Use low shear pumping, such as reciprocating diaphragm pump or gear pump.   6. Long term UV irradiation can reduce the viscosity of carbomer resin.   7. Packing: it can be sealed with moisture-proof and airtight plastic bags, and then packed in cardboard boxes. Carbomer 940 has strong hygroscopicity. During storage, attention must be paid to sealing, and care must be taken to avoid moisture absorption and caking in the exposed air. If there is slight caking, it can become loose powder by gently patting and pressing, which will not affect the thickening effect. After use, it is better to put it in a sealed container with desiccant.   8. Transportation: carbomer is polyelectrolyte, sometimes static when rubbing or extruding resin particles. In case of splashing, remove the dry powder with a broom first. Do not rinse with water. Otherwise, it will form very slippery gel on the ground or equipment.   Hubei xindesheng company specializes in providing carbomer 940, 980 products and after-sales technical support. If you have any questions, please call for details.

1. As an inducer and self-oxidant Luminol is a light inducer and self-oxidant. It can be used as a free radical detection reagent as well as a photosensitizer. In PCL analysis, auto-oxidation is inhibited by a single or a group of antioxidant compounds in the nanomolar range. The antioxidant potential of the sample can be measured by studying the lag phase at different concentrations. 2. Detection of blood luminescence reagent The blood absorbs ultraviolet rays and turns black. After chemical treatment, the blood will also fluoresce. Luminol is commonly used to produce this chemiluminescence, which is a reagent that reacts with catalase in hemoglobin in the blood. 3. Time interval used to identify skeletal remains As the femoral PMI increases, chemiluminescence decreases. In femurs with a PMI of 1 month to 3 years, strong chemiluminescence is observed after a few seconds. Patients with a PMI of 10-15 years have clear chemiluminescence, and 80% of the samples are visible to the naked eye; femurs with a PMI of 25-35 years have weak chemiluminescence in 33% of the samples. Femurs with a PMI of 50-60 years have only a weak response observed in a single femur. No chemiluminescence was observed in femurs whose PMI was over 80 years old. 4. Detect the influence of serum prostate specific antigen The prostate-specific antigen in serum can be detected by luminol chemiluminescence enzyme immunoassay, which has high sensitivity, simple and quick operation, and strong specificity. When tPSA is greater than 10ng/ml, prostate cancer is highly suspected. When tPSA is between 4-10ng/ml, it is called gray value, and the diagnosis of prostate cancer should be combined with the ratio of fPSA/tPSA. 5. Bioluminescence imaging for inflammation Luminol can achieve highly sensitive bioluminescence imaging of inflammation through the luminescence reaction with myeloperoxidase MPO produced in the inflammatory area, which is important for the diagnosis of neurodegenerative diseases, atherosclerosis, cancer and other diseases significance.

Enzymes are the soul that catalyzes various reactions in nature. Many originally difficult chemical reactions can be easily handled by enzymes that are invisible to the naked eye, playing the role of superheroes in daily life. The greater the ability, the greater the responsibility; then what kind of enzyme preparation can be a superhero? The time to test them has arrived. The first level: higher specific vitality The ratio of enzyme activity to enzyme weight is called enzyme specific activity. This is a bit like a weightlifting competition. Two players with the same weight, whoever can lift a heavier weight wins. Why should we examine the specific vitality? Because the higher the specific activity, the less enzymes are needed to accomplish the same job. Enzyme itself is also a kind of protein, which can be a nutrient for microorganisms. The more enzymes also represent the more nutrients, the greater the possibility of breeding microorganisms. Therefore, the higher the specific activity of the enzyme, the lower the possibility of microbial contamination, which is vital to the stability of the catalytic reaction system. The second pass: good thermal stability Like food, enzyme preparations have a shelf life. This is because the specific activity of the enzyme preparation will slowly decrease over time, and when it drops to a certain level, the enzyme cannot continue to perform its expected function. In a high temperature environment, the enzyme activity will decrease faster. Thermal stability is an important reference for judging enzymes. Enzyme preparations with good thermal stability can withstand the toss of environmental temperature changes and have more obvious advantages in terms of validity and transportation. The third pass: a wide range of pH stability pH is a parameter describing the degree of acidity and alkalinity of the aqueous solution. Enzymes have different catalytic activities in different pH environments; enzyme preparations can maintain stable activity within a certain pH range, and over-acid or over-alkali will reduce the stability of the enzyme. If an enzyme can maintain high activity in a wide pH range, it means that the enzyme has good compatibility with catalytic reaction systems of different pH and is the first choice for consideration. Fourth hurdle: specific substrate specificity One must be dedicated to being a human being, and dedicated to doing enzymes. If an enzyme preparation is not selective to the substrate, the products of the catalytic reaction will be diverse and uncontrolled. A specific enzyme preparation will only convert substances with the same structure into products. Even if it is surrounded by other substances, it will only "love" the only substrate, and there will be no stories that shouldn't happen. The fifth level: strong anti-interference ability In the catalytic reaction system, in addition to enzymes, other substances will be added as needed; the raw materials participating in the reaction will also carry certain impurities. The appearance of these substances will affect the activity of enzyme preparations. Even with the appearance of interfering substances, excellent enzyme preparations will continue to show good vitality and continue to play a core role in inducing reactions. Enzyme preparations that can pass these five tests have the basic qualities to become a superhero. However, the distance allows these superheroes to exert their powerful catalytic ability, and there is still a long way to go. In the future, Desheng Technology will continue to introduce other knowledge about enzymes for you.

Biological buffers are an important part of electrophoresis technology and are generally added to the solution to stabilize the pH when current is passed through the sample. In addition, the buffer can also provide the ions needed for electrophoretic migration. The ideal buffer for electrophoresis depends on the isoelectric point of the sample being analyzed. Although there are many pre-made buffers for electrophoresis on the market, it is recommended to use the biological buffers prepared by yourself to make your own solutions. Our company is a professional manufacturer of biological buffers. For this reason, we have created a list of biological buffers frequently used in electrophoresis, so that it is convenient to see which biological buffer is more suitable for you. 1. Tris buffer Used for: gel electrophoresis Useful pH range: 7.5-9.0 pKa (25°C): 7.8-8.2 Molecular weight: 121.14 2. MOPS buffer Used for: gel electrophoresis Useful pH range: 6.5-7.9 pKa (25°C): 7.0-7.4 Molecular weight: 209.3 3. Bicine buffer Used for: gel electrophoresis Useful pH range: 7.6-9.0 pKa (25°C): 8.1-8.5 Molecular weight: 163.2 4. CAPS buffer Used for: Capillary Electrophoresis Useful pH range: 9.7-11.1 pKa (25°C): 10.2-10.6 Molecular weight: 221.32 5. CAPSO buffer Used for: gel electrophoresis Useful pH range: 8.9-10.3 pKa (25°C): 9.4-9.8 Molecular weight: 237.32 It can be seen from the above that there are many biological buffers used in electrophoresis, and there are some differences between them, and they can be used in different electrophoresis. In the current market, there are many vendors of biological buffers, and product specifications and quality are uneven. Our company is a manufacturer specializing in the production of biological buffers. It has been established for more than ten years and has very rich R&D and production experience and product knowledge. It can provide customers with a large amount of technical support and after-sales guarantee. Hubei Xindesheng Materials Co., Ltd. can also provide customized packaging and other services according to customer needs, and our products can be delivered to most areas at home and abroad. In addition, our company also produces blood collection tube additives, chemiluminescence reagents, chromogen substrates, carbomers and other products. If you are interested in our products, you can enter our official website to contact customer service for details.

3 - (cyclohexylamine) - 1-propanesulfonic acid (CAPS buffer) is a kind of sulfamate, cas1135-40-6. Its molecular formula is c9h19no3s, and its molecular weight is 221.32. It is white crystalline powder. Amphoteric, the most widely used is in water-based polyisocyanate coatings and buffer used in enzyme chemistry and HPLC separation of basic drugs. Desheng caps products In biological buffer: buffer for enzyme chemistry and HPLC separation of basic drugs. Product application: in biological experiments, it is an important pH stabilizing reagent, usually choose the appropriate weak acid and its conjugated base to get the appropriate pH value. Most of the biological reactions take place under neutral conditions, generally pH is between 6-8, and the effective buffer range of buffer is also between 6-8. In addition, the acid-base form of buffer should not chelate with some metal ions, such as in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit. In the new coatings, 3 - (cyclohexylamino) - 1-propane sulfonic acid (CAPS) reacts with aliphatic polyisocyanate (the former is zwitterionic sulfamate) under mild conditions and in the presence of tertiary amine neutralizer, and the sulfonylurea derivative is an excellent emulsifier. Without considering the salt group, CAPS modified polyisocyanate has good storage stability and is not cloudy. Even if it contains less sulfonate base group, it can also get very good emulsion in water. A series of ionized polyisocyanates can be obtained, which can be used in various environment-friendly high-quality waterborne two-component polyurethane coatings. These coatings can be compared with general solvent based coatings in terms of dryness, curing and chemical resistance. In addition to the above applications, it is also a raw material for manufacturing welding materials, air conditioning equipment and lithium metal. It is also used for making pyrotechnics, analytical reagent, heat exchange carrier, pharmaceutical industry, air conditioning, pyrotechnics, dry battery and lithium metal, as well as flux and desiccant. From these applications, I can see that 3 - (cyclohexylamine) - 1-propanesulfonic acid plays an extraordinary role in our daily life. We Desheng company is to find these advantages, it has been 15 years of R & D and production, hoping to meet the needs of customers.