China Wuhan Desheng Biochemical Technology Co., Ltd Company News

There are many kinds of anticoagulants. Although they are all for anticoagulation, the anticoagulants to be selected in different tests are also different. How to choose the right anticoagulant in the process of use is very important. Otherwise, the wrong choice may lead to the deviation of test results, which may be very different for patients. Now Desheng takes you to understand the anticoagulant mechanism of different anticoagulants.   Heparin is widely distributed in almost all tissues such as lung, liver, spleen and the granules of mast cells and basophils around blood vessels. It is a mucopolysaccharide with sulphuric acid group, with an average molecular weight of 15000 (2000-40000).   Heparin is the best anticoagulant in the determination of blood chemical composition. Its anticoagulant mechanism is to inhibit the interaction between factor IX a, VIII and PF3 at low concentration together with anticoagulant II, and to enhance the inactivation of serine protease by antithrombin III, so as to prevent the formation of thrombin; it also inhibits the self catalysis of thrombin and the inhibition of factor X. Heparin anticoagulant should be used in a short period of time, otherwise the blood can coagulate if it is placed too long.   Ethylenediaminetetraacetic acid (EDTA) has disodium, dipotassium and Tripotassium salts. EDTA salt had little effect on the morphology of red blood cells and white blood cells.   EDTA is one of the most common and important anticoagulants and reagents in clinical work. Its mechanism is to prevent blood coagulation by forming a stable chelate with calcium ion in water phase. EDTA can also affect platelet aggregation and leukocyte phagocytosis, and is not suitable for hemostatic test and platelet function test. The salts of EDTA include potassium, sodium and lithium, which are soluble in water. The solubility of potassium is higher than that of sodium. The potassium salt of EDTA is the best for whole blood cell count.   Citrate can form a soluble chelate with calcium ions in blood, thus preventing blood coagulation. It was used with blood in the ratio of 1:9 or 1:4.   Citrate is mainly sodium citrate. Its anticoagulant principle is that it can combine with Ca2 + in blood to form chelate, which makes Ca2 + lose coagulation function, and the coagulation process is blocked, so as to prevent blood coagulation. Sodium citrate 6mg can anticoagulate 1ml blood, strong alkaline, not suitable for blood test and biochemical test.   Oxalate is also a common anticoagulant with the advantage of high solubility. The commonly used oxalate anticoagulants are sodium oxalate, potassium oxalate and ammonium oxalate. The concentration of sodium oxalate is 0.1 mol / L, and the ratio of sodium oxalate to blood is 1:9.   After oxalate dissolves, the dissociated oxalate and Ca2 + in the sample form calcium oxalate precipitation, which makes Ca2 + lose the coagulation function and block the coagulation process. Oxalate can cause platelet aggregation and affect the morphology of leukocytes, so it can not be used for the differential count of leukocytes and platelets.

Sodium citrate, also known as sodium citrate, is an organic compound, a sodium salt, its chemical formula is na3c6h5o7, the appearance is white to colorless crystal, with the taste of soapy water. It is soluble in water and glycerin, but insoluble in alcohols and other organic solvents. Deliquescence, weathering in hot air.   In terms of structure, citric acid is a kind of tricarboxylic acid compound, which has similar physical and chemical properties with other carboxylic acids. When heated to 175 ℃, citric acid will decompose to produce carbon dioxide and water, leaving some white crystals. Citric acid is a kind of strong organic acid, with three H + ions which can be ionized. It can be decomposed into a variety of products by heating, and react with acid, alkali and glycerol.     When it comes to sodium citrate, people's first reaction is to use it for anticoagulation in vitro. When fresh blood is taken in clinic, some sterilized sodium citrate needs to be added to prevent blood coagulation, so sodium citrate is called anticoagulant. Sodium citrate has other applications in addition to anticoagulation in vitro. Next, Desheng will take you to know about it.   1. Used in chemical industry   Citric acid can be used as chemical analysis reagent, as experimental reagent, chromatographic analysis reagent and biochemical reagent, as complexing agent and masking agent, and as buffer solution.   2. For environmental protection   Sodium citrate buffer is used for flue gas desulfurization. China is rich in coal resources, which is the main part of energy. However, there has been a lack of effective flue gas desulfurization process, resulting in serious air SO2 pollution. It is urgent to study effective desulfurization process. Citric acid sodium citrate buffer solution is a valuable desulfurizer because of its low vapor pressure, non toxicity, stable chemical properties and high SO2 absorption rate.   3. For cosmetics   Citric acid is a kind of fruit acid, its main role is to accelerate the regeneration of keratin, commonly used in emulsion, cream, shampoo, whitening products, anti-aging products, acne products, etc. The renewal of cutin is helpful to the peeling off of melanin in the skin, the thinning of pores and the dissolution of blackheads.   4. Used in medicine   Calcium must be involved in the formation of prothrombin activator and subsequent coagulation. Citrate ion and calcium ion can form a soluble complex which is difficult to dissociate, thus reducing the concentration of calcium ion in blood and blocking blood coagulation. It is often used as anticoagulant in vitro in blood transfusion or laboratory.   Desheng specializes in R & D, production and sales of Blood collection tube additive, in vitro diagnostic reagents, biological buffers and luminescent substrates. In the aspect of blood vessel additive, it has formed independent intellectual property rights and professional production and research capacity. Can produce a variety of additives, blood samples anticoagulant series products include heparin sodium, heparin lithium, sodium citrate, EDTA dipotassium, EDTA Tripotassium, potassium oxalate, etc.; blood samples anticoagulant series products include blood coagulant powder, blood coagulant, etc.; blood samples pretreatment materials include separation gel, silicide, etc.

Heparin is a kind of mucopolysaccharide widely existing in mammalian tissues. It mainly exists in mast cells and has anticoagulant effect. It is widely used in surgery and the treatment of cerebral thrombosis and myocardial infarction. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant, heparin sodium has been paid attention to all over the world. It is also one of the main export drugs in China.   With the deepening of research, it has been found that heparin sodium not only has anticoagulant, antithrombotic and lipid regulating effects, but also has anti-inflammatory, anti allergic, anti-virus, anti-cancer and other functions. At present, heparin sodium is mainly extracted from the small intestinal mucosa of pigs, sheep and cattle lungs. Studies have shown that heparin sodium is the highest content in the small intestinal mucosa of pigs. Crude heparin sodium is a traditional export product of China and occupies an important position in the world.     This paper will introduce a kind of extraction process of heparin sodium, which is simple to operate, high yield of heparin sodium, and suitable for industrial application   (1) Raw material treatment: clean the fresh small intestine with clean water, scrape the small intestine mucosa after cleaning, and then put the small intestine mucosa into the blender until it is minced for standby;   (2) Heating enzymolysis: put the chylous intestinal mucosa obtained in step 1 into the heating tank, add water, mix and stir, then add 8% trypsin and weak alkaline solution in turn, adjust the pH value until the pH value of the solution is 8-10, and then heat it. During the heating process, keep the pH value between 8.5-9.5, heat it to 30-40 ℃, keep the constant temperature for 2-3 h, and then continue to heat it to 50-60 ℃, keep the constant temperature for 10 h -After 20 min, the filtrate was obtained by hot filtration;   (3) Cooling and adsorption: the filtrate obtained in step 2 is cooled to 30-40 ℃ to remove the floating oil on the upper layer of the filtrate, then the resin is added to stir the adsorption for 6-7h, and the resin is filtered out after the adsorption is completed;   (4) Elution: put the collected resin into the elutor for washing, add 10% sodium chloride solution, keep the temperature at 50-55 ℃, stir for 3-4 hours, and collect the eluent; elute twice, and combine the eluent collected twice for use;   (5) precipitation: filtrate the two collected eluent into the settling tank, add the alcohol with a mass fraction of 80-85% to stir evenly, then adjust the pH value to 7-8, and seal the precipitate for 10-12 hours.   (6) Dehydration and drying: the precipitate is put into a drying oven and dried to obtain heparin sodium.   As a preferred scheme, the ratio of water to small intestinal mucosa in step (2) is 1:3. As a preferred scheme, the set temperature of the drying oven is 50-60 ℃, and the drying time is 3-5h.   In short, the above extraction steps make the small intestine fully contact with trypsin by stirring the small intestine into a chyma, and then use the process of enzymatic hydrolysis heating to maximize the activity of trypsin, fully extract heparin sodium, and improve the yield of heparin sodium; in the precipitation process of the invention, the impurities are removed by filtration to obtain a clean precipitate, so as to improve the purity of heparin sodium and improve the yield of heparin sodium Desheng is a professional manufacturer of heparin sodium, the potency is generally between 150iu-180iu, welcome to consult and purchase.

What is nucleic acid detection, that is, after collecting human secretions, under laboratory conditions, through the analysis of virus RNA gene sequence, using PCR amplification method for detection, clinical etiology diagnosis. At present, nucleic acid detection is the most effective method to determine whether the patient is infected with the virus as early as possible, and to detect and treat the virus as early as possible.   Nucleic acid test swabs are divided into two types: nasal swab and pharyngeal swab. During the sampling process, the doctor will use a swab like a cotton swab to wipe the secretion around the pharynx, tonsil or nasal cavity. As long as it is "gently wiped", it is quick and painless. Medical institutions will disinfect the test area, and medical staff will also disinfect their hands to avoid cross infection. Generally speaking, the sampling process is safe and clean, and there is no need to worry about the risk of infection.   Two types of virus preservation solution of Desheng So what are the types of swab preservation solution after sampling? Swab preservation solution can be divided into inactivated and non inactivated. At the beginning, almost all the swab preservation solutions used in the market are direct preservation of live virus, that is, non inactivated swab preservation solution. This preservation method will lead to higher infection risk for medical staff in sampling, transportation and detection. In view of this situation, anti epidemic measures should be taken It is very necessary to use the swab preservation solution to inactivate the virus directly.   It should be noted that: while inactivating the virus, we should also consider whether the preservation solution can stably preserve the integrity of the virus nucleic acid, so as to avoid the degradation of the nucleic acid in the sample before detection, resulting in "false negative" nucleic acid detection. The inactivated virus preservation solution produced and developed by Desheng can avoid this kind of problem.   In short, nucleic acid detection swab preservation solution can be divided into two categories. The inactivated preservation solution produced and developed by Desheng is a swab preservation solution with cleavage function, which contains cleavage salt of inactivated virus and cleavage protein to protect nucleic acid. Virus preservation and cleavage are completed in one step.   The other is the non inactivated preservation solution. On the contrary, it can protect the integrity of protein and nucleic acid of the virus. In addition to nucleic acid detection, it can also be used for virus culture research. The operating environment requirements of the two preservation solutions are not the same. The inactivated environment does not need to be so strict. Because of the risk of virus infection, the operating environment requirements of the non inactivated type are different Higher.

Serum separation gel is a kind of hydrophobic organic compound, it is a viscous fluid, its structure contains a lot of hydrogen bonds, because of the association of hydrogen bonds to form a network structure. Under the action of centrifugal force, the network structure is destroyed and becomes a low viscosity fluid. When the centrifugal force disappears, the network structure is formed again and the fluid with high viscosity is recovered.   Serum separation gel is a material used to separate serum (plasma) and blood cells. Its main purpose is to form an isolation layer between blood cell components and serum, prevent material exchange between blood cells and serum, ensure the original characteristics of blood samples within a certain period of time, and make the detection results more accurate. In the field of clinical laboratory, no matter in clinical chemistry, serology, immunology, most of the samples need to be separated from the serum. It can also be used with heparin, EDTA and sodium citrate.     The key factors of separating gum are as follows   A. Specific gravity: 1.045-1.065g/cm3, between the specific gravity of serum (plasma) and blood cells. The PrP tube has two specific gravity of 1.055 and 1.078, which are used to separate platelet and serum components respectively;   B. Viscosity: between 100000-200000 Li poise (dynamic viscosity measured by rotary viscometer).   C. Thixotropy: when an object (such as a coating) is sheared, its consistency decreases. When it stops shearing, its consistency increases. When it is sheared, its consistency increases. When it stops shearing, its consistency decreases. That is, the property of changing at one touch. This is the key to the separation layer formed by centrifugal force.   D. Physiological inertia: the separation gel is in direct contact with the blood, and can not react with the blood, otherwise it will affect the blood composition, cause significant differences in test results, and lead to misdiagnosis. Blood cell is a kind of special delicate material, a little careless will most likely cause hemolysis, affect the accuracy of test results.   E. Radiation resistance: the blood collection vessel is required to be sterile, and gamma ray irradiation is generally used for sterilization. After gamma ray irradiation, the properties of the gel can not change significantly, including specific gravity, viscosity, thixotropy and physiological inertia. In general, gamma ray is produced by cobalt 60, and the radiation dose is generally in the range of 8-25kgy; the radiation dose is determined by the initial colony number of blood collection vessel.   G. Stability: on the one hand, it requires the stability of the separation adhesive after long-term storage, and it should be stable for at least two years, and the physical and chemical properties should not change significantly. In addition, there is no reaction between the separation gel and various additives in the blood collection vessel, which affects the effectiveness of the reagent and thus affects the blood detection.   Serum separation gel is still a glue with viscosity, and the viscosity has a certain relationship with temperature. When the temperature is too low and the weather is cold, the viscosity of serum gel increases, and the process of adding glue is difficult. In winter, the glue is heated. No problem under 80 ℃. Desheng has never stopped in the blood vessel additive. We want to use our products to tell all customers that your choice is right.

Acridine esters have been widely used in the field of life science, mainly because of their high luminous efficiency, mild reaction conditions, only H2O2 and dilute alkali are needed, and no catalyst is needed to stimulate chemiluminescence (CL). The CL reaction mechanism of these compounds is characterized by the dissociation of the luminescent group and the modified group, the luminescent efficiency is not limited by the length of the connecting arm, and the Photofragmentation effect is small. Through the modification of its structure, the possibility of acridine esters involved in the establishment of ultramicro immunochemical analysis was greatly increased.     Application of acridine ester   1. Determination of tissue type plasminogen activator activity by acridine ester luminescent method Thrombotic disease is one of the main diseases endangering people's life safety. Among several fibrinolytic drugs, tissue type plasminogen activator (t-PA) is favored by the medical community because of its specific fibrinolytic effect. Many tissues of the body contain t-PA, but it can not be extracted in large quantities because of its small amount. Genetic engineering products have been used in clinic in the world, and the development of t-PA genetic engineering products in China is stepping up. It is urgent to establish a sensitive, simple and low-cost detection method.   At present, acridine esters have been successfully synthesized in China, and the properties of acridine esters have been comprehensively analyzed, which provides a favorable condition for the establishment of a sensitive and low-cost quantitative method for t-PA activity. This method was used to determine the expression of RT PA in human melanoma t-PA (MT PA) cells and Chinese hamster ovary (CHO) cells, and the results were consistent with that of fibrin agar plate assay (FAPA). The changes of t-PA activity in plasma of healthy people before and after venous blood flow occlusion were significantly different. The activity of plasma t-PA in patients with lung cancer was significantly higher than that in normal people. Therefore, this method is expected to be used in clinical diagnosis and basic theoretical research of some diseases.   2. A new chemiluminescence system of xanthine oxidase acetaldehyde acridine   Acetaldehyde is oxidized to acetic acid by oxygen in the air under the catalysis of xanthoxygenase, and hydrogen peroxide is produced at the same time; hydrogen peroxide produced by enzyme reaction oxidizes 10-methyl-9-methylphthalophenyl acridine ester fluorosulfonate in alkaline medium to produce chemiluminescence.   3. Application of acridine ester labeled antibody in determination of antibody level of tuberculosis patients   Acridine antibody conjugates can be used for the determination of antibodies produced by bacteria, viruses and human autoimmunity. At present, there are at least 30 kinds of autoimmune diseases. As long as the antigen of the disease is prepared and used to coat the solid phase, the acridine ester human IgG antibody marker can be used for determination, which provides meaningful information for clinical diagnosis and pathogenesis research.   Desheng has six kinds of acridine ester products with different groups: acridine ester dmae-nhs, acridine ester nsp-dmae-nhs, acridine salt nsp-sa-nhs, acridine salt nsp-sa-nhs, acridine hydrazide nsp-sa-adh, acridine ester me-dmae-nhs. In addition to acridine ester series of chemiluminescence reagents, we also have luminol and isoluminol and other products.

Now there are various cosmetic brands, and their components are various. You may as well look at the major cosmetic components, and you will find that there is a "shadow" of carbomer. Such as eye gel, Estee Lauder, Laneige sleep mask and so on, all of which will undoubtedly use carbomer modulated gel.   Carbomer is a crosslinked acrylic polymer, which can be neutralized by alkaline material to form transparent gel after being dissolved in water. After carboxy group is ionized by carbomer neutralization, the molecular chain is dispersed and extended due to mutual repulsion of negative charges, showing a state of great expansion and viscosity. It can produce highly effective thickening effect at very low dosage.   The low concentration of additives (such as POM) can make the oil insoluble and rheological. Therefore, Carbomer is widely used in personal care products, such as skin care, hair care products, toothpaste and other products. Therefore, Carbomer can be found in many international cosmetics.     Carbomer is the key raw material for making skin care products and glue. After the neutralization, the carbomer gel is easy to absorb, and has a very important influence in skin care products. It can make the various ingredients in skin care products integrated and make them fit in a stable state. What is the magic card's function in cosmetics?   1. Skin care   Carbomer has a significant maintenance effect on human skin. It has a certain infectious power on human skin. It is usually applied in skin care products added with Carbomer, which can maintain the skin, reduce the irritation and damage to human skin and skin mucosa, and avoid a variety of allergic symptoms.   2. UV resistant   Carbomer has a certain specificity, it can improve the resistance of human skin to ultraviolet light after wiping on the surface of the body skin, and reduce the damage of ultraviolet light on the body skin. The sunscreen isolation cosmetics added carbomer has a very ideal sunscreen isolation effect, can avoid rough skin, and can also avoid the skin being burned by ultraviolet light.   3. Reduce viscosity   Carbomer has a certain degree of looseness, and it is a kind of slightly acidic material with strong water absorption. When making skin care products, Carbomer can be added in an appropriate amount. It can reduce the consistency of this material and keep their characteristics stable. In today's industrial production, Carbomer is the key raw material for making skin care products and skin care products.   4. Anti inflammation and sterilization   Carbomer is also a kind of pure natural medicinal value ingredient, which can eliminate inflammation and bacteria. Carbomer eye drops sold in the pharmaceutical market today are therapeutic drugs made of carbomer as the key raw material, which have excellent effect in removing inflammation around human eyes.   5. Ensure the quality of skin care products   Carbomer is an organic chemical neutralizer, which has a very key influence in the production of skin care products. It can make a variety of ingredients in skin care products integrated together, and make them in a stable and suitable situation. The skin care products added carbomer will have excellent cultivation substrate, and you will feel very comfortable after wiping on the skin.   Desheng is a manufacturer of carbomer in China. Its carbomer series products include carbomer 940 and 980. It has been proved by many tests that all indexes meet international standards. Desheng cabom has been exported to more than 20 or 30 countries, and has rich experience in export. If you need to solve the problem or need, you can click the official website to consult our customer service.

MOPS is a zwitterionic biological buffer. It is a white powdery solid at room temperature, with high hydrophilicity and excellent water solubility (1000 g/L at 20°C). When MOPS is dissolved in water, the appearance of its aqueous solution is colorless. This is just its basic information, if you want to know more, then you have to look down.     What is the recommended use of MOPS?   1. The operation that can separate RNA by agarose gel electrophoresis; 2. Used for protein purification in chromatography; 3. Measure absorption in ultraviolet/visible spectrophotometry and use cyclic voltammetry to study redox characteristics; 4. The electron transfer mechanism in nitrogenase; 5. Separate nucleic acid and protein by electrophoresis; 6. In the control of medium pH value of 5, including cell culture medium for yeast, bacteria and mammalian cells; 7. It has been used as a buffer component of charcoal yeast extract culture medium; 8. It interacts with the peptide backbone of bovine serum albumin, resulting in a net stabilization of the protein.   What questions should you consider before choosing MOPS for your research?   1. When used for mammalian cell culture, the MOPS buffer concentration should not be higher than 20mM 2. Although most studies have found that there is almost no complex between the mop and the metal, some studies have found that interference may occur due to the formation of metal complexes; 3. It can change the interaction of lipids; 4. MOPS can affect the thickness and barrier properties of the rat endothelial surface layer; 5. It interacts with DNA and forms a complex; 6. It can slightly affect the mRNA expression of bovine embryos produced in vitro; 7. It can be oxidized by H2O2, but due to its slow oxidation, it is not expected to have a significant impact on the biological system; 8. In the presence of glucose, autoclaving can partially degrade MOPS.

The determination of free fatty acid (FFA) in serum is an important biochemical test index, and FFA is closely related to human health. Because serum components are complex and there are many types of FFA, the detection is affected by many factors, so the chromogen substrate TOPS is usually used to determine FFA.   Chromogen substrate TOPS reagent   Chromogen TOPS determination FFA steps:   1. In the container, first add the buffer solution weighed according to the formula ratio, and then add ATP, coenzyme A, 4-aminoantipyrine, ion activator (magnesium chloride), stabilizer (BSA), and preservative in sequence. After adding proper amount of water, add surfactant, adjust the pH to pH 6-9 with concentrated hydrochloric acid, add acyl-CoA synthase at last, then stir and filter, and add distilled water to the filtrate to obtain reagent A quantitatively.   2. Add buffer to the container, then add chromogen TOPS, water, surfactant in turn, adjust the pH to pH 6-9, finally add HRP and acetyl CoA oxidase, then stir and filter, and then add distilled water to the resulting filtrate to a quantitative amount Reagent B is obtained.   3. Fill the above reagents into vials and store them at 2-8°C. Take the reagent A and the sample and mix them in a certain ratio, incubate for a certain period of time, and read the absorbance value A1; add the reagent B and mix evenly, incubate for a certain period of time, and read the absorbance value A2. FFA concentration in the sample=standard solution concentration Χ(ΛΑ_/ΛΑ_#), ΛΑ_=Α2-A1.   Prepare buffer:   In a clean glass container, first add HEPES buffer (Good's buffer, zwitterionic buffer, including HEPES, Tris, MES, MOPS, etc.) that has been weighed according to the formula ratio, add appropriate amount of water, and then add surfactant 5ml/ L TritonX-100, use concentrated hydrochloric acid to adjust the pH to pH 6-9, the amount is about 5ml/L, then stir and filter, and then add distilled water to the obtained filtrate to a quantitative amount.   The method of using TOPS as a chromogen substrate to detect serum or plasma FFA has high accuracy and small measurement error, and it is the most suitable color among the many Trinder's chromogens, which is low in acetyl CoA, high in stability, and large in molar absorption coefficient. original. In addition to TOPS, the chromogen substrates produced by Desheng include TOOS, MAOS, ADPS, etc., which are suitable for the detection of blood sugar and other biochemical indicators.

As a common biological buffer, Tris base is not only widely used as a solvent for nucleic acid and protein, but also one of the main components of protein electrophoresis buffer. In addition, Tris can also produce a variety of cosmetics, chemicals, pesticides, medicine, coating preparation and other products, and is an important intermediate for the preparation of surfactants, vulcanization accelerators, reducing agents and some drugs.   1. Used as medicine: if it is often used in acute metabolic and respiratory acidemia, Tris base is an amino acid buffer, which can absorb hydrogen ions and correct acidosis.   2. For synthesis: Tris is used to prepare surfactants, vulcanization accelerators and intermediates of some drugs. Because it has three equal hydroxyl groups, it can also be used as a good reducing agent. In the alkaline condition of sodium hydroxide, it can reduce chloroauric acid solution to prepare stable gold nanoparticles. This method is non-toxic and pollution-free, and belongs to green reduction method.   3. Tris as reducing agent: under alkaline condition, gold nanoparticles can be prepared by ultrasonic reduction of chloroauric acid solution without adding other stabilizers. The environmentally friendly and biocompatible gold nanoparticles were synthesized. Gold nanoparticles with different sizes can be prepared by adjusting the experimental conditions.   4. Neutralizer: the most common function of Tris in cosmetics is to adjust pH value (pH value). It can also be used as a neutralizer to neutralize Thickener (carbomer), so as to reduce the sticky feeling, improve the respiratory efficiency of skin cells, and prevent pore blockage. In addition, Tris can be used as an odor regulator in cosmetics containing amine salts to regulate the odor of amine produced by rubbing when applying cosmetics, so as to avoid the release of any residual or persistent odor.   5. Coating preparation: Tris mainly plays the following four roles in the coating preparation process: pH regulator, cross-linking accelerator, polyester coating raw material and phase change core material.   Desheng has 20 years of experience in developing and producing biological buffers, blood collection additives, color reagents and chemiluminescence reagents, and can provide high-quality Tris raw materials. In addition to Tris, HEPES, CAPS, MOPS and PIPES, our company is actively developing new fields, providing nucleic acid detection materials, virus preservation solution and gel free raw materials, Carbomer, and calls for detailed consultation.

With the formation and continuous development of environmental science, a new environmental monitoring technology has emerged. Chemiluminescence analysis is a common analysis method, which is mainly used in modern testing technology in environmental monitoring. According to the intensity or total amount of radiation produced by chemical reaction, the corresponding component content can be determined by chemiluminescence analysis. Chemiluminescence analysis is an independent analytical technology in atmospheric environmental monitoring, which can effectively analyze trace and trace amounts. In China, after years of in-depth research in chemiluminescence analysis. It is proved that this technology has become an important technology in many aspects, such as air pollutants research, atmospheric environment monitoring, etc. Luminol is one of the earliest and most widely used chemiluminescence reagents. The chemiluminescence reaction of luminol needs to be carried out under alkaline conditions. For example, it can be oxidized by some oxidants, and the maximum emission wavelength of luminol is 425nm. Generally, the oxidant used is hydrogen peroxide. Luminol chemiluminescence system has been successfully used to determine the concentrations of SO2, Co, O3, HS and NOx in the air for a long time. In addition, the chemiluminescence reaction between luminol and hydrogen peroxide is very slow in the absence of catalyst, otherwise it is very fast. The most commonly used catalysts are metal ions. In a large concentration range, the higher the concentration of metal ions, the stronger the luminous intensity. Therefore, the chemiluminescence analysis of some metal ions can be carried out. Using this reaction, the organic compounds containing metal ions can be analyzed, achieving high sensitivity. Environmental monitoring involves many kinds of pollutants in the analysis gas, involving a wide range of aspects, so it needs high sensitivity, wide linear range, and the use of fast and simple detection method. Chemiluminescence analysis has its own unique advantages, which can well meet the above requirements and is widely used in atmospheric environmental monitoring. In order to be able to better adapt to atmospheric environmental monitoring, chemiluminescence analysis will have a good prospect in the following aspects. The application research of chemiluminescence in environmental monitoring and analysis has been developing day by day. With the continuous development of chemiluminescence instruments with high degree of automation, sensitivity and selectivity, and the development and launch of commercial monitoring instruments, the application of chemiluminescence in environmental monitoring will be popularized and promoted rapidly.

It is a kind of raw material for color development in the biochemical kit, CAS number: 82692-96-4, with high water solubility, is a stable aniline analog, the pH range of the color process and oxidation reaction is very wide, Suitable for diagnostic testing and biochemical tests.   Application   1. It has several advantages over conventional color-producing reagents in the colorimetric determination of hydrogen peroxide activity.   2. The new Trinder's reagent is stable enough, and can be used in both solution and test assembly line detection system;   3. In the presence of hydrogen peroxide and peroxidase, during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH), very Stable purple or blue dye;   4. The molar absorbance of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA;   5. The amount of substrate can be determined by the color development of the oxidative coupling reaction.   Detection method   1. Prepare sample solutions for enzymatic oxidation reactions. The pH range of the buffer should be 5.5-9.5.   2. Use the same buffer to prepare a standard solution containing a known amount of substrate.   3. Add the appropriate unit of oxidase to the sample solution, and then add an equal volume of the detection solution.   4. Incubate the mixture at room temperature or 37°C for 30 minutes to 1 hour.   5. Prepare a standard curve and determine the concentration of the substrate in the sample solution.   Precautions   1. This product needs to be sealed and stored in a dry and cool place, and it needs to be packaged in a dark brown bottle with a better protection against light;   2. ADOS is a white crystalline powder, if the color becomes darker, it may have grown bacteria or partially oxidized, so it cannot be used;   3. ADOS powder may adhere to the wall of the tube when it is dissolved. Use a centrifuge to centrifuge at low speed to reduce product loss;   4. The product has good solubility and can be directly dissolved in deionized water; double distilled water or ultrapure water can be used for higher experimental requirements.