China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The principle and classification of chemiluminescence reagents, there are three types of luminescence in nature: physical luminescence, chemiluminescence and bioluminescence. The narrow sense of luminescence immunoassay mainly refers to chemiluminescence. Chemiluminescence is an oxidation chemical reaction. After the electron is excited, it returns to the ground state and releases energy in the form of photons. The number of released photons is used to reflect the intensity of the chemical reaction. The commonly used luminescent agent is aminophthalic hydrazide (isoluminol and luminol). Connaught), acridine esters. Chemiluminescence immunoassay is a non-radioactive immunoassay method that has developed rapidly in the past 30 years, and it is a kind of ultra-high-sensitivity micro-assay technology. It combines with the immune response through the chemiluminescence system, and uses chemiluminescence-related substances to label antibodies or antigens. After reacting with the antigen or antibody to be tested, the free chemiluminescence markers are separated, and other related substances of the chemiluminescence system are added. Chemiluminescence, quantitative or qualitative detection of antigen or antibody. Advantages of chemiluminescence immunoassay: (1) High sensitivity: theoretically the highest sensitivity of chemiluminescence can reach 10-18Mol/L. (2) Short detection time: The time for measuring the optical signal of each sample is no more than a few seconds, and it can be completed within 9-60 minutes from sample addition to analysis result. (3) Wide detection range: Theoretically, the linear range can be up to 105 relative luminous units (RLU), which is 5 orders of magnitude. (4) The analytical method is simple and fast: most analytical determinations are in a one-step mode in which only one reagent (or a composite reagent) is added. (5) High sensitivity: more than RIA. (6) Good safety and long service life: exempt the use of radioactive materials. So far, its hazards have not been discovered; the reagents are stable, and the shelf life can be up to six months to more than one year. Disadvantages of chemiluminescence immunoassay: (1) The light-emitting process is short (2) High background (3) High instrument failure rate (4) Poor reagent stability (5) The detection accuracy is not high Application of chemiluminescence immunoassay: Chemiluminescence immunoassay has unique characteristics of high sensitivity, high specificity, speed, accuracy, specificity, and automation. It plays an important role in clinical testing, drug analysis, environmental monitoring and other fields. Especially the determination of various hormones, tumor markers, drug concentration and other trace biologically active substances. The clinical application of chemiluminescence immunoassay is mainly reflected in the treatment of thyroid hormones, adrenal/pituitary hormones, anemia factors, tumor markers, infectious diseases, allergic diseases, therapeutic drug monitoring, etc., to ensure the stability of the data results reliable. Among them, the detection of tumor markers has certain application value in the early detection of tumors, the development of the disease, the evaluation after treatment, and the monitoring of recurrence and metastasis. Desheng Biotechnology has been doing chemiluminescence reagents for many years. In addition to chemiluminescence reagents, there are also other series of products such as blood collection tube additives, biological buffers, and chromogen substrates. Chemiluminescence reagents have been developed and produced by Desheng Biotechnology for many years. The products can fully meet the requirements of various manufacturers, with reliable quality and preferential prices. If you are interested, you can call to discuss in detail.

Chemiluminescence is molecular electromagnetic radiation caused by light produced by a chemical reaction. Because of its high sensitivity, good specificity, wide application range, simple equipment and wide linear range, chemiluminescence reactions have received extensive attention in various fields of life sciences, detective detection, food safety, and drug analysis. A good chemiluminescence reaction is determined by the intensity and sensitivity of the reaction. The following is a brief analysis of the influencing factors. Chemiluminescence labeling chemistry and labeling quantitative chemistry require high specific activity, and therefore require high detection sensitivity of the label material itself. The detection limit of conventional enzyme labels or fluorescent derivatives is about 1014 mol, which has been successfully applied to low-sensitivity measurement systems. The lower detection limit of luminol and acridine ester salts is about 10-18 mol of the available luminometer, so it matches or even exceeds the sensitivity of 1251. Luminol and protein will greatly affect the quantum yield of the reaction. In particular, aminobutylethyl isoluminol can be combined with low molecular weight compounds without reducing the quantum yield. The compulsory method of first dissociation of the complex is to use the luminescence method. Quantum yield is an important indicator of the observation results when isoluminol is used to label hepatitis B antibodies. When designing a detection scheme, there are many known chemiluminescence reactions to choose from. However, only a small number of known reactions have entered large-scale clinical diagnostic testing methods. There are several factors that influence the suitability of a particular chemiluminescence technique for automated immunoassays. First, the reaction should show sufficient chemiluminescence quantum yield. All aspects of detection chemistry, including labeling methods, triggers, chemiluminescent reagents, are durable and reproducible. The method using enzyme labeling also faces another requirement, that is, the enzyme must be quite stable and easy to conjugate without reducing its catalytic activity. The ideal chemiluminescence reagent has an easy-to-measure signal through effective chemiluminescence reaction and predictable time course of luminescence. Desheng Bio is committed to providing a variety of high-quality chemiluminescence reagents for the life science industry, including luminol, isoluminol, DMAE-NHS/Me DMAE-NHS/NSP-DMAE-NHS /NSP-SA/ NSP- SA-NHS /NSP-SA-ADH/. In order to further promote the development of innovative biomarkers in different fields to meet your basic, application and clinical research needs.

In recent years, chemiluminescence immunoassay methods have been increasingly favored by people due to their high sensitivity, strong specificity, wide application range, simple equipment required, and wide linear range. They are used in life sciences, clinical medicine, environment, and food. , Medicine and other fields have been widely used. Chemiluminescence immunoassay is an analysis technique that combines highly sensitive chemiluminescence detection with highly specific immune response to detect various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. What is chemiluminescence immunoassay? Chemiluminescence analysis is an analysis method that determines the content of a substance based on the intensity of the radiant light produced by a chemical reaction. Chemiluminescence immunoassay is to combine the chemiluminescence system with the immune response, label antibodies or antigens with chemiluminescence-related substances, and after reacting with the antigen or antibody to be tested, the free chemiluminescence markers are separated and added to the chemiluminescence system. Other related substances produce chemiluminescence for quantitative or qualitative detection of antigens or antibodies. The four most commonly used markers in chemiluminescence immunoassays are luminol, isoluminol and their derivatives, acridinium ester derivatives, peroxidase and alkaline phosphatase. Classification of chemiluminescence immunoassay: According to the different labels and luminescence principles used in chemiluminescence, it can generally be divided into three categories: direct chemiluminescence immunoassay, enzymatic chemiluminescence immunoassay and electrochemiluminescence immunoassay. Different from the former two, electrochemiluminescence generates light signal by electrochemical reaction of electrochemiluminescence agent on the surface of the electrically activated electrode. Co-reactants are often needed to improve the luminous efficiency, such as tripropylamine (TPA) as ruthenium terpyridine ([ Rb(bpy)3]2+) electron donor co-reactant. Commonly used light-emitting systems are: acridine esters, luminol (luminol), terpyridine ruthenium, peroxy oxalate (TCPO, DNPO) and strong oxidant potassium permanganate, Ce (SO4) 2 etc. (see Chart 1 below). Some luminescent agents react slowly with oxidants, and they also need the role of catalysts and enhancers to improve luminous intensity and stability. Application of chemiluminescence immunoassay: The chemiluminescence immunoassay method not only has the high sensitivity of chemiluminescence analysis, but also has the high specificity of immune response. It has a wide range of applications and development prospects in the fields of clinical diagnosis, food safety and environmental monitoring. 1. Fast chemiluminescence immunoassay method Traditional immunoassay methods often take several hours to complete. The long incubation time also leads to the adsorption of immunoreagents on the inner wall of the pipeline, which increases the signal crossover between different assays, thus limiting the detection throughput of samples and the effectiveness of immunoassay methods. Value. In order to overcome these shortcomings and achieve high throughput of immunoassays, the external field drive or effective solution mixing strategy can be used to accelerate the mass transfer rate of antigens, antibodies and other biological macromolecules, or increase the temperature of the reaction system to accelerate the kinetics of the immune response. , So as to achieve rapid immune detection. 2. Multi-component chemiluminescence immunoassay method The simultaneous realization of multi-component detection in a single analysis process has outstanding advantages such as high analysis throughput, short time required, less sample consumption, and low analysis cost. It is the long-term goal pursued by immunoassay and is also the research of immunoassay in recent years Hot spot. 3. Highly sensitive chemiluminescence immunoassay method In actual detection, the sensitivity and specificity of the existing detection methods are not ideal, and high-sensitivity and high-selectivity detection methods are increasingly showing their importance. The development of nanotechnology provides an opportunity for the development of highly sensitive chemiluminescence immunoassay methods. Nanoparticles have been widely used in biomarker analysis, and there have been a variety of signal amplification methods used in the construction of highly sensitive immunoassay methods.

Luminol, also known as luminol. The chemical name is 3-amino-phthalic hydrazide. It is a pale yellow powder at room temperature, which is a relatively stable synthetic organic compound. Luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract. Luminol is a chemiluminescent reagent, which can be converted into excited amino phthalic acid in the presence of hydrogen peroxide molecules, and then emits strong fluorescence. Under normal circumstances, the chemiluminescence reaction of luminol and hydrogen peroxide is quite slow, but when there is a catalyst, the reaction is very rapid. The most commonly used catalysts are metal ions. Within a wide range of concentrations, the concentration of metal ions is proportional to the luminescence intensity, which enables chemiluminescence analysis of certain metal ions. This reaction can be used to analyze organic compounds containing metal ions. Achieve high sensitivity. The second is to use certain compounds to determine the enhancement and inhibition of luminol on chemiluminescence. The initial application of luminol reagent was to detect proteins. In western blotting, an enzyme-linked antibody is often used to bind the protein to be detected, and the combination of luminol chemiluminescence reagent and enzyme (peroxidase) can make the local luminescence and reduce the protein The position is displayed. The luminescence in the picture is very bright, probably because of the chemiluminescence enhancer added to the reagent. Of course, the luminescence is far less bright when actually detecting proteins. In addition, luminol derivatives such as isoluminol (ABEI) are labeled on carboxylic acid and ammonia compounds, and then separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then under alkaline conditions It reacts with hydrogen peroxide-potassium ferricyanide to perform chemiluminescence detection, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection. Desheng's current chemiluminescence reagents include acridinium ester, luminol, etc., and the quality is guaranteed and the supply is sufficient.

There are often friends who are very curious about the principle of luminoluminescence. Desheng also talked about it before. If you are interested, you can learn about it on the Desheng news page. I will not elaborate here. After understanding the principle of luminol's chemiluminescence, will you be curious about its luminescence detection method? What are the three major luminescence detection methods of luminol? The three major luminescence detection methods of luminol are adding catalyst to accelerate luminescence, adding inhibitor for indirect determination, and indirect determination by coupling. 1. Add a catalyst to speed up the luminescence method: Adding a catalyst is a commonly used method at present. Under normal circumstances, the chemiluminescence reaction of hydrogen peroxide and luminol luminescence system is very slow, but after adding some catalysts, the reaction becomes very rapid. Someone here will ask whether the existence of these catalysts will affect the entire system. De Sheng wants to say that the presence of a catalyst means that at the end of the reaction, the nature and quantity of the catalyst remain unchanged, that is, relative to the entire reaction, the catalyst does not participate. At the same time, these catalysts can be detected at a certain concentration, and the impact on the entire detection is almost negligible. At present, the main catalysts of luminol include some metal complexes and transition metal ions. The metal complexes mainly include hemoglobin and peroxidase; the transition metal ions mainly include Fe3+, Fe2+, Mn2+, Cr2+, etc. The catalyst that we often use in chemiluminescence immunoassay is peroxide, especially horseradish peroxidase. Some compounds can be immunoreacted with peroxidase-labeled antibodies, and then chemiluminescence assays can be performed with luminol to determine these compounds or antibodies. Such as digoxin, hepatitis B surface antigen, etc. are all detected and analyzed by this method. 2. Indirect determination method by adding inhibitor: In addition to the catalyst’s acceleration of detection, some inhibitors were also found in the research. These organic compounds can inhibit the chemiluminescence of luminol, such as reducing compounds containing phenolic hydroxyl groups, which can react with oxidants during the reaction and reduce the oxidants. The concentration of, the intensity of chemiluminescence is reduced, so as to indirectly measure this kind of organic substances. 3. Over-coupling indirect measurement method: Finally, the method Desheng will introduce is to perform indirect measurement through coupling reactions. Coupling reactions here refer to combining one reaction that can produce or consume chemiluminescent reactants with another chemiluminescent reaction, so that certain substances can be realized. Indirect chemiluminescence determination. For example, some substrates produce hydrogen peroxide under the action of certain enzymes and then produce chemiluminescence with luminol. By measuring chemiluminescence, the purity of the measured substrate can be indirectly known. Desheng is a high-tech enterprise focusing on the R&D and production of chemiluminescence reagent, blood collection tube additives, biological buffers and color substrates. It has formed independent intellectual property rights and professional production research and development capabilities in blood collection tube additives. Provide products and raw material solutions for more than 100 domestic and foreign manufacturers. Chemiluminescence products include luminol and 6 types of acridinium esters.

How much is RMB 40 billion? I believe that most people, like me, are a relatively abstract concept, simply a big number. Suppose you win 5 million prizes in the lottery, and you win 8,000 times. If it is used to buy in vitro diagnostic reagents, how much can you buy?   How much serum separation gel can be bought for 40 billion? Usually the price of domestic serum separation gel is tens to one or two hundred per kilogram. If we calculate at 100 yuan per kilogram, 40 billion can buy 400 million kilograms of separation gel. Separating glue is generally 25 kilograms per barrel, and the barrel is about half a meter high. These barrels of separating glue can be connected to 8 million meters, which is a thousand times the height of Mount Everest and 800 times the depth of the Mariana Trench, the deepest part of the earth.   How much can be done if these separating glues are added to the vacuum blood collection tube? Generally, coagulation tubes or anticoagulation tubes with a blood collection volume of 3-5mL add 0.8-1.2g of separation gel. We calculate by adding 1g separation gel per tube. These separation gels can be made into 400 billion blood collection tubes with a tube length of 10 cm. , Then these blood collection tubes can be connected from the earth to the moon back and forth 50 times, and can also go around the earth's equator 1,000 times, and weave a scarf for the earth.   In Vitro Diagnostic Reagents How much heparin can be bought for 40 billion? If you think the separation glue is not intuitive enough, then use 40 billion to buy heparin, the price of this is much higher. We calculate at a price of about 100 yuan per gram. The money can buy 400,000 kilograms of heparin. On average, 1300 pig small intestines can extract 1 kilogram of heparin. This heparin needs to sacrifice 520 million pigs. These pigs are 4.3 of the total population of Japan. It is 1.6 times the total population of the United States and 70% of the entire European population! My goodness, if so many pig small intestines are used to extract heparin, the remaining pork is even more unimaginable.   Of course, we can’t really spend 40 billion to buy separation gel or heparin. There are many in vitro diagnostic reagents that are more expensive than heparin, such as chemiluminescence reagent acridinium ester and enzyme preparations, antigen-antibody protein preparations, and the price is even higher than that of heparin. The milligram calculation is far more than that of gold, but usually the amount of these reagents is not much at one time, so the entire supporting products involved are also many. Desheng is a manufacturer engaged in the research and development of blood testing and virus testing related reagents, and welcomes the cooperation of in vitro diagnostic reagent companies.

Carbopol 940 , this seemingly ordinary white loose powder, actually plays an extremely important role in the cosmetics and pharmaceutical fields. Its strong hygroscopicity and unique acidic properties make it a multifunctional raw material. The acidic group content in its molecular structure is as high as 52-68%, which gives it a certain acidity and also makes the pH value of its 1% aqueous dispersion 0. When carbomer is neutralized by alkaline substances, its molecular chains will exhibit a dispersed and extended state due to the repulsion of negative charges, thereby exhibiting astonishing expansion and viscosity. Among the many variants of Carbopol, the Carbopol 940 is undoubtedly a brilliant star. As a white powder cross-linked polyacrylic polymer, Carbopol 940 not only has extremely high rheological modification ability, can produce unimaginable viscosity, but also can form bright and transparent gel or hydrogel, even cream texture. This makes it an indispensable part of the cosmetics and pharmaceutical industry. Carbopol 940, as a water-soluble thickener, has a first-class effect. Not only that, it can also serve as a suspension agent, stabilizer, and emulsifier, providing comprehensive protection for products. Especially in advanced cosmetics and medicinal excipients, the transparent matrix of Carbopol 940 brings unique texture and visual effects to the product. However, it is not easy to make transparent gel with Carbopol 940. Due to its swelling process when combined with water, it is necessary to give sufficient time for it to fully dissolve in water. If the time is too short, carbomer is easy to agglomerate, affecting the quality of gel. In addition, other components in the system are also factors that need to be considered. Many natural plant ingredients, such as flower water and extracts, contain electrolytes, which may affect the performance of Carbopol 940. Next, let's take a closer look at the process of Carbopol 940 configuring transparent gel. Firstly, add an appropriate amount of Carbopol 940 to the beaker, moisten with a small amount of alcohol, and pre disperse. Then, soak the pre dispersed Carbopol 940 in water at 80 ℃. It should be noted that even without stirring, Carbopol 940 can dissolve basically within 4-6 hours. This is because its special structure makes it easy for water to penetrate and expand. After the preparation of Carbopol 940 solution, other components can be mixed with Carbopol. In order to adjust the pH value of gel and make it more transparent and viscous, alkaline substances such as triethanolamine or tea can be used for neutralization. However, it should be noted that the amount of triethanolamine is difficult to control, and excessive use may dilute the system. Therefore, it is recommended to use diluted tea (such as a 10% aqueous solution) for neutralization. During the addition process, observe the changes in the system while stirring. When the system becomes more transparent and the state becomes more viscous, its pH value can be tested. Hubei Xindesheng Materials Co., Ltd. is located in the United Science and Technology City of Gedian Development Zone, Ezhou City, Hubei Province. It is a high-tech enterprise that focuses on the research and development, production, sales, and technical services of biochemical reagents and polymer materials. The Carbopol 940 it produces has won widespread trust from customers for its excellent transparency and stable quality. As an enterprise with import and export rights, Xindesheng not only enjoys a high reputation in the domestic market, but also actively explores the international market and provides high-quality products and services to global customers.

Blood collection tube additives are the core raw materials in blood collection tubes, and their quality directly determines whether the clinical test can be performed in time, the accuracy of the test results and the reliability of the diagnosis results. As a manufacturer of blood collection tube additives, Desheng has the responsibility and obligation to provide customers and patients with additives of stable quality to ensure the accuracy and timeliness of the test results. In the past, separation gel was mainly used for serum biochemical testing, that is, separation gel and blood coagulant were used in conjunction. With the development of blood collection tube technology and inspection requirements, more and more blood collection tubes have begun to use separating gel test tubes. At present, there are blood collection tubes (separation gel + potassium salt anticoagulation tube), electrolyte test tubes (separation gel + heparin salt), blood coagulation test tubes (separation gel + sodium citrate) and other varieties of blood collection tubes that use separation gel. These aspects have driven the increasing use of separating glue. But in the process of using it will always encounter various problems. A. Difficulty in adding glue: mainly because the temperature is too low. In winter, the temperature drops, the weather is cold, and the viscosity of the separating glue increases, making the process of adding glue more difficult. On the one hand, adding glue in winter is solved by heating. Separating glue generally can withstand high temperatures below 80°C without any problem. Now many equipment manufacturing companies' glue adding machines are equipped with heating function, which provides solutions for blood collection enterprises. In addition, some problems can be solved by reducing the viscosity of the separating glue, but it is not a fundamental solution. B. Flowing: After the separation glue is added, when the test tube is placed flat, the separation glue flowing distance will be too large, and some even flow to the nozzle of the tube. This is because the intermolecular chemical forces of the separating glue have not been fully recovered during the process of adding glue, and the network state has not been formed. The first solution is to increase the thixotropy of the separating glue, but it will cause difficulty in adding glue; the second is to put it upright for a few hours, and then lay it flat after the thixotropy of the separating glue recovers. C. Bubble problem: After adding glue and vacuuming, bubbles will appear in the separating glue, or bubbles will also appear after irradiation sterilization. This is because the separation glue clamps air invisible to the naked eye during the glue adding process. After being heated under vacuum or irradiation sterilization, the air in the separation glue slowly expands and becomes visible bubbles. In the process of separating glue production and adding glue, it is necessary to reduce air entrapment in the separating glue as much as possible. D. The problem that the separation gel does not turn over: Some separation gel blood collection tubes will not turn over clinically, usually because the centrifugal force is not enough, or the centrifugation time is not enough. Increasing the centrifugal force or prolonging the centrifugal time will basically solve the problem. There are also some separation glues that do not turn over due to factors such as aging, which is a quality problem of the separation glue. E. Flip the separating gel to the serum: This is caused by the too small specific gravity of the separating gel. In recent years, this situation has basically not occurred. Our company once caused this situation due to detection errors around 2010, which caused great problems to customers and the company suffered a lot. F. Detector alarm: The alarm problem of separation gel blood collection tube often occurs in clinical testing.In the past, the industry always thought that it was caused by the oil droplets or fragments of the separating glue. After years of tracking and analysis, we have found the root cause of this problem. The alarm of the instrument is usually centrifuged under the condition of incomplete blood coagulation, causing the fibrin filaments to float in the serum. When the probe is aspirated, the fibrin filaments are sucked into the probe, causing the instrument to block and alarm.

Dipotassium ethylenediaminetetraacetate is abbreviated as EDTA-2K. Its appearance is white crystalline powder. It is soluble in water and slightly soluble in alcohol. Its aqueous solution has a pH of about 5.3 and a melting point of 272 °C. Dipotassium ethylenediaminetetraacetate has a wide range of uses. It can be used to complex metal ions and separate metals. It is also used in detergents, liquid soaps, shampoos, agricultural chemical sprays, antidotes, and is commonly used in blood anticoagulants. Dipotassium ethylenediaminetetraacetate (EDTA-2K) Dipotassium ethylenediaminetetraacetate can be used for complete blood counts. Whole blood cell analysis is widely used in clinical testing. Platelet count has become an important basis for the diagnosis and treatment of clinical thrombosis and bleeding diseases, and it is also one of the important parameters for preoperative detection of surgical patients. The International Committee for Blood Standards recommends the use of dipotassium ethylenediaminetetraacetate (EDTA-K2) anticoagulant blood for complete blood counts. Studies have also explored the detection of total bilirubin (TBIL), direct bilirubin (DBIL), total protein (TP), albumin (ALB), and alanine in anticoagulated plasma and serum of dipotassium ethylenediaminetetraacetate. Differences in the results of 8 liver function indexes including aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT), alkaline phosphatase (ALP) and so on. Methods The above eight biochemical tests were performed on EDTA-K2 anticoagulated plasma and serum on an automatic biochemical analyzer, and the results were compared and evaluated. The results showed that EDTA-K2 anticoagulated plasma compared with serum, the results of TBIL, TP, ALB, ALT, AST were not statistically significant, EDTA-K2 anticoagulated plasma ALP was significantly lower than that of serum, the difference was statistically significant, and GGT was slightly lower In serum, the difference was statistically significant, and DBIL was significantly higher than that in serum, and the difference was statistically significant. Therefore, EDTA-K2 anticoagulated plasma can be used for the detection of TBIL, TP, ALB, ALT, and AST. It is not suitable for the detection of DBIL and ALP. For the GGT test results, the reference range of EDTA-K2 plasma should be formulated or multiplied by the correction factor. Application of dipotassium ethylenediaminetetraacetate (EDTA-2K): 1. Additives for vacuum blood collection tubes: The additive of vacuum blood collection tubes is an aqueous solution of dipotassium ethylenediaminetetraacetate (EDTA-2K), and 4mg of dipotassium ethylenediaminetetraacetate (EDTA-2K) is required for anticoagulation of 2ml of blood. Because the concentration is small, in order not to dilute the blood, 20μl of an aqueous solution containing 200g/L of ethylenediaminetetraacetate (EDTA-2K) 200g/L is generally pre-installed in each blood collection tube. The validity period of the blood collection tube is two years. When the operating environment changes slightly, such as temperature changes, water can easily evaporate to the pipe wall (especially the water in the plastic PET pipe solvent can penetrate the pipe wall and leak out), causing the EDTA-2K in the pipe to crystallize and crystallize quickly When collecting blood, the latter dipotassium ethylenediaminetetraacetic acid blood collection tube is required to be turned upside down at least 8 times in order to fully dissolve and mix the crystallized EDTA-2K in the blood. If the action is too large, the red blood cells will be destroyed and hemolyzed. Gathered and adhered and broken. 2. Prepare toilet deodorant. In parts by mass, it includes the following substances: 5-10 parts of acid salt, appropriate amount of ammonia, 50-70 parts of alcohol, 5-10 parts of urotropine, appropriate amount of dipotassium edetate, appropriate amount of isobutanolamine, water 60 to 80 copies. The deodorant can not only deodorize but also prevent scale, and the method of use is simple, the dosage is small, and the duration is long, and it has no corrosive effect on the toilet. The production method is simple, the raw material cost is low, and it is non-toxic, harmless and non-polluting. 3. Used as a complexing agent in liquid phase analysis. If the invention provides a tetrabutylammonium hydrogen sulfate buffer salt system for liquid chromatography detection, the solvent is water, and the solute includes tetrabutylammonium hydrogen sulfate and a buffer ion pair. The buffer ion pair is composed of potassium dihydrogen phosphate and phosphoric acid The solute also includes a complexing agent, and the complexing agent is preferably dipotassium ethylenediaminetetraacetate. The tetrabutylammonium hydrogen sulfate buffer salt system provided above is stable within 24 hours without turbidity. Taking the analysis of related substances of moxifloxacin hydrochloride as an example, it can be seen that the tetrabutylammonium hydrogen sulfate buffer salt system stored at room temperature for 24 hours The chromatographic behavior of moxifloxacin, N-methyl impurity, impurity A, impurity B, impurity C, impurity D, and impurity E on the reversed-phase chromatography column is basically the same as that of the newly configured tetrabutylammonium hydrogen sulfate buffer salt system. 4. Prepare metal polishing agent. The polishing agent is based on dipotassium ethylenediaminetetraacetate (EDTA-2K), trisodium phosphate, dibutyl phthalate, sodium silicate, inorganic acid, dicarboxylic acid compound, tetrapolyricinoleate, biological Buffer, stabilizer, water-soluble surfactant, water as raw materials, and through a scientific content ratio, the polishing agent prepared from this can effectively remove impurities on the metal surface. It is not only convenient to operate, but also has a good polishing effect. Improve the production efficiency, and ensure the cleanliness and gloss of the metal surface, and improve the quality of the metal. Desheng has been committed to the research and development of blood collection tube additives. It has 15 years of production experience and can provide customers with lithium heparin, sodium heparin, dipotassium EDTA and tripotassium EDTA. If you need it, please call for details.

The full name of HEPES buffer is 4-hydroxyethylpiperazine ethanesulfonic acid, CAS is 7365-45-9, HEPES is often used in biological buffers, pH buffer range: 6.8-8.2, the main component of hepes buffer is hydroxyethylpiperazine ethyl Sulfuric acid, HEPES is an amphoteric buffer, which is soluble in water and does not form stable complexes with metal ions. It has a good buffering capacity in the pH range of 7.2-7.4. It is mostly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Used in a variety of biochemical reactions: 1. HEPES buffer is often used as a buffer reagent in the cell culture medium of various types of organisms, cell-cell adhesion, short-term cell aggregation and culture, and buffer for cleaning tissues and cells; 2. In protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; 3. In DNA research, PIPES is used as a buffer for calcium phosphate and DNA precipitate formation system, and as a buffer for AFM and electroporation experiments. 4. In addition, HEPES has a certain interference to the reaction between DNA and restriction enzymes, and it is not suitable for Lowry's method to determine protein content. 5. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. 6. The reaction buffer, pre-hybridization buffer, and hybridization buffer for separating and analyzing RNA nuclear components; used for RNA and T4RNA. Molecular biology grade is used to label the 3'-end of RNA with T4 RNA ligase, separate and analyze the components of the reaction buffer, pre-hybridization buffer and hybridization buffer of nuclear RNA. HEPES powder HEPES related issues 1. The pH required for most cells is 7.2-7.4, but the appropriate pH for cell culture varies with the types of cells being cultured. Fibroblasts prefer a higher pH (7.4-7.7), while the passage of transformed cell lines requires acidity. pH (7.0-7.4). Since most culture fluids are buffered by the system of sodium bicarbonate (NaHCO3) and CO2, the CO2 concentration in the gas phase should be in equilibrium with the sodium bicarbonate concentration in the culture fluid. If the CO2 concentration in the gas phase or in the incubator air is set at 5%, the amount of NaHCO3 added in the culture solution is 1.97g/L; if the CO2 concentration is maintained at 10%, the amount of NaHCO3 added in the culture solution is 3.95g/L. The cell culture bottle cap should not be screwed too tightly to ensure gas exchange; 2. The pH value of the culture solution using HCO3 and CO2 buffer pair is unstable and tends to be alkaline after storage for a certain period of time. If the pH of the culture fluid changes too quickly, HEPES buffer can be added to the culture fluid to a final concentration of 10-25mM; 3. HEPES is an amphoteric buffer, mostly used in biological research such as oxidative phosphorylation, protein synthesis in a sterile environment, photosynthetic phosphorylation, CO2 fixation, etc.; HEPES has no effect on the substrates of metal ionase and is suitable for transmission In electron microscopy (TEM); in cell culture media, the advantage is that it can maintain a relatively constant pH value during open culture or cell observation. HEPES (25mM) can be used as a substitute for bicarbonate buffer, or as a Addition of bicarbonate buffer (10-15mM) to relieve the restriction of high-concentration CO2 culture environment. Dissolved CO2 and bicarbonate are also very important for good cell growth.

The impact of the epidemic will cause some things to fall and inevitably some things will rise, such as Carbomer. As a cross-linked acrylic polymer, carbomer has good thickening performance and strong suspending ability. In addition to being used in hand sanitizers, it is also widely used in skin care lotions, creams, transparent gels, and hair styling. Gel can even be applied to batteries. The main functions of Carbomer: 1. Thickening-can produce a wide range of viscosity and fluidity2. Suspension—make insoluble components permanently suspended in the system3. Emulsification—plays emulsification and stabilization in the oil/water phase Carbomer thickening mechanism: a. Salt thickening, the most common method is to change the acidic resin into an appropriate salt, so that the curled resin molecules are opened to cause thickening. In water and other polar solvents, use NaOH, KOH, NH40H Such neutralization can easily generate salt; in acute weak or non-acute solvents, organic amines should be used for neutralization. When the resin is used as an emulsifier, in order to achieve the best stability of the oil/water emulsifier, it is necessary to double neutralize the resin with a water-soluble inorganic base and an oil-soluble organic amine, so that the product is soluble in water and oil. Salt acts as a bridge between the oil phase and the water phase. b. Light bond thickening, adding a hydroxyl donor to the resin, the resin molecule as a carboxyl donor can combine with one or more hydroxyl groups to form a hydrogen bond to thicken. This method takes time, which may range from 5 minutes to several Hours, the consistency can reach the highest value. The PH value of this kind of material is slightly acidic, and the dispersion can be heated to 70°C (but should not exceed) to speed up the thickening. Commonly used polyhydroxy and polyethoxy reaction agents: non-ionic surfactants, solvents, polyols, glycol-silane copolymers, polyethylene oxide, fully hydrolyzed polyvinyl alcohol, etc. use: Carbomer has excellent thickening, gelling, adhesive, emulsifying, suspending and film-forming properties. As a thickener and emulsifier, it has the following applications in the pharmaceutical industry: 1. Controlled-release drugs made as slow-release materials, such as ascorbic acid, aspirin, lithium carbonate, atropine sulfate, procaine hydrochloride, chlorpheniramine, quinine sulfate, theophylline and so on. 2. The application in the formulation of external medicines, as a carrier matrix to make ointments, suppositories, creams, gels, emulsions, etc. 3. Utilizing the gel, adhesive and film-forming properties of this product, the application in bio-adhesive, as a carrier matrix and bio-attachment made of drugs, it stays in the tissue mucosa for a long time and improves the bio-adhesion of the drug. Utilization, such as targeting mucous membranes, including ocular, nasal, intestinal, vaginal and rectal mucosa. 4. Utilizing the suspending ability of this product, it can effectively suspend insoluble components to form a uniform dispersion system, which is used in oral suspensions, which is safe and effective, avoids odor, maintains stability, and improves bioavailability. In order to increase production capacity, Desheng actively applies new equipment and expands production staff. While ensuring production capacity, it must also pay attention to product quality. Carbomer series products that are in high demand are Carbomer 940 and Carbomer 980. Desun can provide high-quality Carbomer series raw materials.

Tris base is named tris(hydroxymethyl)aminomethane; tromethamine; tromethamine; 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.   Tris is a weak base, and its pKa is 8.1 at 25°C; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is about 10.5. Generally, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of the pH value. But at the same time, attention should be paid to the influence of temperature on the pKa of Tris.   Tris is often used as a biological buffer, and is often formulated with pH values ​​of 6.8, 7.4, 8.0, and 8.8. Its pH value varies greatly with temperature. Generally speaking, for every degree of temperature increase, the PH value drops by 0.03. tris structure 1M Tris-HCl 6.8 and 1.5M Tris-HCl 8.8 are the most commonly used reagents for SDS-PAGE. And TAE, TBE, etc. prepared by Tris are the most commonly used reagents for DNA electrophoresis, and TE (pH 8.0) is mainly used to dissolve DNA. (TE is the collective name of Tris and EDTA.)   Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fiber of C. elegans nuclear lamin (lamin). Tris is also one of the main components of protein running buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.   The Tris buffer developed and produced by Desheng is used for the preparation of buffers in biochemistry and molecular biology experiments; the preparation of pharmaceutical intermediates and the preparation of kits.