China Wuhan Desheng Biochemical Technology Co., Ltd Company News

4-Hydroxyethylpiperazine ethanesulfonic acid (HEPES buffer) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research.     As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products:   1. Advantages of PH range:   The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture.   2. Purity advantage:   Desheng HEPES has a purity of ≥99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services.   3. Advantages of reserves:   Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment.   4. Export advantages:   Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention.   5. HEPES packaging advantages:   We can pack in quantities according to customer needs. Generally, there are 500g small bottles and 25kg cardboard drums. The big packaging is covered with plastic bags, the white powder is packed in the belt, and the tie is tight.   6. Recommended storage:   HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not degrade due to autoclaving. However, if the HEPES aqueous solution is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H2O2) will be produced. Therefore, it should be noted that the HEPES aqueous solution must be kept away from light to avoid affecting the experimental results. HEPES powder is generally placed in a dry room when it is placed, and cannot be exposed to direct sunlight for a long time. It is best to store it at room temperature 2-8°C.   Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.

The full name of EDTA-2K is dipotassium ethylenediaminetetraacetate. It is a white crystalline powder and belongs to a common complexing agent. Because it can complex with the calcium in the blood to inactivate the calcium and further cause the blood to not coagulate, it is usually used to prepare anticoagulants and mainly used in the blood collection tube industry. EDTA has two CAS numbers, mainly because one of them is actually EDTA dipotassium crystalline hydrate. People often mistake the CAS numbers of these two EDTAs, which makes people confused. The real situation is that the CAS number of EDTA-2K is 2001-94-7 and the CAS number of EDTA-2K dihydrate is 25102-12-9. The difference between them is that there are two crystal waters in one molecule. And this article is an introduction to the wide range of uses of EDTA-2K. The International Blood Standards Committee recommends the use of EDTA-2K anticoagulated blood for complete blood counts. The use of EDTA-2K as an anticoagulant in vacuum blood collection tubes can preserve leukocyte hypertrophy in a short time, prevent platelet aggregation, and preserve platelets. In addition to being an anticoagulant, EDTA-2K has many uses in industrial production, environmental optimization and other fields. EDTA-2K can be used as a new type of gel material magnesium phosphate cement retarder, compared with borax used in traditional magnesium phosphate cement. It has a better retarding effect, can prolong the setting time of the magnesium phosphate repair system, and ensure the strength of the magnesium phosphate cement, making it more widely used in the rapid repair of highways, bridge decks, and airstrips. In oilfield production, EDTA-2K is also used as a stabilizer to form a desulfurization synergist, which can not only maintain the stability of the desulfurization synergist, but also chelate the calcium and magnesium ions in the operating system to be treated to stabilize the overall operating system. Water quality. The desulfurization synergist can effectively chemically react with the sulfide in the equipment to achieve the purpose of removing sulfide, have a good desulfurization effect, and ensure the safety of oilfield construction. EDTA-2K is also commonly used to prepare deodorants, such as toilet deodorants, decomposing pesticides and odor molecule removers, etc. In addition, according to the description of related patents, the potassium salt of ethylenediaminetetraacetate is calcined at high temperature, pickled, and washed. Activated carbon can be prepared in processes such as washing and drying.

There are many kinds of blood anticoagulants in blood collection tube reagents, and one anticoagulant has a wide range of uses, that is, potassium EDTA. There are many types of EDTA potassium salt, among which dipotassium is used more, but what about its principle and application? There should be some people who are not very familiar with it. Here we can take a look at what application areas and principles it has.     EDTA dipotassium salt is also known as dipotassium ethylenediaminetetraacetate dihydrate (EDTA-K2). It is a white crystalline powder, easily soluble in water, easy to absorb moisture, and has a molecular weight of 404.6. DETA dipotassium salt can protect the cell components of the blood, does not affect white blood cells, has the least impact on red blood cells, and can inhibit the aggregation of platelets, which is suitable for general blood tests. But for the separation test with platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor is it suitable for calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine amino peptide Enzyme determination and PCR test.   The dipotassium EDTA is mostly used in complexing metal ions and separating metals, as well as washing powder, shampoo, liquid soap, agricultural chemical spray, blood anticoagulant and other fields. The dipotassium EDTA is also recommended by the International Committee for Standardization in Hematology as the preferred anticoagulant for blood count and classification.   The principle of EDTA dipotassium salt as an anticoagulant, first of all, we first understand the principle of blood coagulation, which can be divided into three parts, 1. The formation of prothrombin activator, 2. The role of prothrombin activator in calcium ion With participation, prothrombin is converted into active thrombin. 3. Soluble fibrinogen is converted into insoluble fibrin under the action of thrombin. Fibrin is shaped like filaments, criss-crossed, and collects a large number of blood cells to form a jelly-like blood clot. This is how coagulation occurs. The dipotassium EDTA has a great affinity for calcium ions in the blood, and can complex with calcium ions so that prothrombin cannot be converted into thrombin, thereby making the blood anticoagulant.

Recently, some customers reported that the EHSPT (also called TOOS) solution prepared by myself was transparent, but it turned yellow or red after being left for two days. What is the reason? Can it still be used? Reasons for discoloration of Trinder's reagent solution: Chromogenic substrates such as EHSPT and MAOS are all new Trinder’s reagents, which are all derivatives of highly water-soluble sodium aniline, which will produce red or yellow substances when oxidized. Trinder's reagent powder is not easy to be oxidized. It needs to be sealed and refrigerated after preparation. If the solution is exposed to the air for a long time and the temperature is high, they may be slowly oxidized by the oxygen in the air. Discoloration of the solution occurred. Trinder’s reagent for enzyme-linked immunoassay Trinder's reagent detection principle: Trinder's reagents such as EHSPT and MAOS can be oxidized by hydrogen peroxide (TOOS, MAOS, etc. need to be coupled with 4-AAP) in the presence of peroxidase to produce red quinone imine substances, which can be detected by measuring absorbance with a photometer The content of the test sample that can generate or oxidize hydrogen. The new Trinder’s reagents are all reductive color-developing reagents. They are originally colorless. When they are catalyzed by peroxidase by oxygen, they can generate colored oxidation products. However, chromogen substrates such as EHSPT are relatively unstable in the solution. They are contaminated by oxidizing impurities or exposed to air for a long time, which will cause them to slowly oxidize, and the solution will change color. Therefore, when the chromogen substrate is formulated into a solution, it is generally recommended to prepare it for immediate use to prevent it from being contaminated or oxidized. The advantages of Trinder's reagent compared to TMB: When using TMB as a color reagent, the detection wavelength is 450nm maximum absorption wavelength and 405nm wavelength, and the absorption wavelength of bilirubin in the serum is 380~530nm, which will interfere with the detection results, and the color should be done under acidic conditions. Reaction limits its application. The UV absorption of the color reaction product of the new Trinder’s reagent is >540nm, MAOS and MADB are even as high as 630nm, and the reaction requires a wide pH range, and is less interfered by other substances in the serum. It can be used for detection items that require high precision values. At present, in addition to chemiluminescence immunoassays, enzyme-linked immunoassays are more commonly used. The new Trinder's reagent is one of the important chromogenic substrates. Desheng is engaged in the R&D, production and sales of acridinium ester chemiluminescence reagents and new Trinder's reagents. All have extensive experience.

In biochemical testing, the detection of blood glucose, blood lipids, liver and kidney function, etc. usually adopts enzymatic photometry. Many detection principles are based on Trinder reaction. The required chromogen substrates and enzymes have requirements for the experiment, and the stability is not ideal. Polymer stabilizers to improve.   The chromogen substrates used in the Trinder reaction, such as TOOS, MAOS, ADPS, etc., belong to the sodium salt of aniline sulfonate. After being formulated into an aqueous solution, it cannot be exposed to the air for a long time and will be slowly oxidized. Therefore, it is usually stored as a powder reagent. Temporarily prepare a solution. On the other hand, enzymes have higher requirements on the temperature and pH of the reaction system, and they are prone to inactivation even when the concentration is too low. So improving their stability is very valuable.   Chromogen substrate MAOS powder   Through research, it is found that a polymer with a special structure can significantly improve the stability of chromogen substrates and enzymes. The polymer has the general structural formula of R1 R2 R3, R1 is selected from olefin unsaturated groups or olefin unsaturated monomers; R2 is selected from ester group COO or other group O; R3 is selected from polyethylene glycol, polyoxypropylene, Polypropylene glycol, polyacryl alcohol and other polymer groups.   The application of this polymer can increase its stability by interacting with enzymes and chromogen substrates, so that a variety of in vitro testing items can maintain the stability of enzymes and chromogen substrates under extreme environments (such as high temperatures). The detection result has less deviation and higher accuracy. It can be combined with other components to form a stable enzyme and chromogen substrate composition. It can be used in enzyme-catalyzed color reaction and in the preparation of in vitro detection products based on the principle of enzyme-catalyzed color reaction. And the preparation of enzymes and chromogen substrate stabilizers.   Applicable enzyme preparations include: peroxidase such as POD or HRP, cholesterol esterase, cholesterol oxidase, alkaline phosphatase, creatine kinase, glycerol kinase, phosphoglycerol oxidase, lipoprotein lipase, urate oxidase, acet One or two or more of acid oxidase, glucose oxidase, creatinine hydrolase, and creatine hydrolase.   Trinder's chromogen substrates suitable for polymer stabilizers include: TOOS, TOPS, MAOS, MADB, etc. and their combinations 4-AAP, MBTH. The above-mentioned chromogen substrates and enzymes are commonly used reagents in enzymatic photometry. Desheng is a manufacturer and can provide a variety of chromogen substrates and enzyme preparations.

The full name of Tris-Hcl in Chinese is Tris Hydrochloride, another name is Tris Hydrochloride, and the English name is TRIS hydrochloride. I believe that many people are familiar with Tris. Tris has a wide range of applications. Tris buffer is not only used as a solvent for nucleic acids and proteins, but also has many important uses.     Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fibers of the nematode nuclear laminin. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard. As a protein buffer, if mass spectrometry is required for subsequent work, Tris is not suitable. Try to replace it with a buffer that can be tolerated by other mass spectrometers.   Tris-HCl is derived from the reaction of Tris as the main raw material. Its solution is acidic and is mainly used as a biological buffer to adjust the pH of the virus preservation solution. If you need to adjust to neutral or alkaline, you can add Tris or NaOH to achieve. In addition to this information, what are the configuration methods for Tris-Hcl? How much do you know? The configuration method of Tris-Hcl will be introduced below.   Preparation method of Tris-HCl buffer:   1. Tris-Hcl (0.05mol/L, 25℃) After mixing 50ml 0.1mol/L tris (Tris) solution with x ml 0.1mol/L hydrochloric acid, add water to dilute to 100ml PH    x/ml     PH     x/ml 7.1    45.7     8.1    26.2 7.2    44.7     8.2    22.9 7.3    43.4     8.3    19.9 7.4    42.0     8.4    17.2 7.5    40.3     8.5     14.7 7.6    38.5     8.6      12.4 7.7    36.6     8.7     10.3 7.8     34.5    8.8     8.5 7.9     32.0    8.9     7.0   Tris molecular weight=121.14: 0.1mol/L solution is 12.114g/L. Tris solution can absorb carbon dioxide from the air, so the bottle cap should be tightly closed when using. When configuring Tris-Hcl solutions with different concentrations and different pH values, you can first prepare a 0.05mol/L solution with a certain pH value, and then dilute to the required concentration.   2. 1M Tris-Hcl (PH7.4, PH7.6, PH8.0) Component concentration 1M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 121.1g Tris and place it in a 1 L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Add the required PH value for the adjustment of the concentrated HCL amount according to the following value; PH value              Concentrated HCL 7.4                        Approximately 70ml 7.6                        about 60ml 8.0                        about 42ml 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving. 3. 1.5M Tris-Hcl (PH8.8) Component concentration 1.5M Tris-Hcl Configuration volume 1L Configuration method: 1. Weigh 181.7g Tris in a 1L beaker; 2. Add about 800ml of deionized water, fully stir to dissolve; 3. Adjust the PH value to 8.8 with concentrated Hcl; 4. Dilute the solution to 1L; 5. Store at room temperature after autoclaving.   It should be noted that the solution should be cooled to room temperature before adjusting the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.   The above is the configuration method of Tris-Hcl provided by Hubei Xindesheng Material Co., Ltd. In addition to the production of Tris-Hcl, Desheng also produces other chemicals, such as blood collection tube additives, enzyme preparations, chemiluminescence reagents, biological buffers, chromogen substrates and carbomer products. At present, Desheng company has been established for decades, has its own R&D team, and has invested a lot of energy in biological buffers. The quality of the products is reliable, the price is favorable, and if you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Since the COVID-19 pandemic, COVID-19 reagents have been on fire, and a little brother, the COVID-19 preservation solution, has been brought along with him. "Suddenly like a spring breeze coming all night", the preservation liquid also springed up like bamboo shoots after a rain. However, "squandering flowers are gradually becoming charming." Faced with hundreds of products, there is really no way to choose. Which points should I look at? 1. Are the raw materials of the virus transport medium qualified? Highly responsible virus preservation liquid manufacturers can not only fulfill their promises, but also ensure that the facts are described without exaggeration, such as the strict selection of raw materials to prevent fraud. The irresponsible manufacturers will only exaggerate in order to promote the order. In this process, customers should keep their eyes open and distinguish right from wrong.   2. Is it easy to grow bacteria? After the preservation solution is stored for a period of time, it is easy to spoil. The clear preservation solution becomes muddy, moldy, and bacteria grow, and it is embarrassing to be unsafe at night. The bacteria that grow out are not bad, I know they can't be used. Those who have been contaminated but will not grow long, hide in which box, which patient they are used on, and what impact they will have on the results, is known to all. Preventing pollution is undoubtedly a big challenge in the production and quality control process. Of course, it is not difficult to deal with, and measures such as cleaning the workshop and adding antibiotics can all be dealt with calmly.   3. Is it easy to leak? Because the virus preservation liquid is liquid, some preservation liquids are prone to leakage when they encounter rough handling or the negative pressure of air transportation during transportation, so the entire box and the whole box have to be scrapped. If the packaging is not in place and leaks, it will be very troublesome. Therefore, the selection of packaging materials, the design of the structure, and the manufacturing process must be checked.   4. Is the price reasonable? The price of any product is a very critical factor. Because of the epidemic, many companies’ conventional products are not selling well. Therefore, many companies in the market will sell shoddy products at low prices in order to survive. The price is low, but the quality is not guaranteed. The price and value are often corresponding. If the price of the virus transport medium is much lower than the market average price, then you must seriously consider whether its quality is reliable. Therefore, when choosing a virus transport medium manufacturer, be careful not to blindly pursue low prices.

Since the beginning of the new coronavirus pandemic, the unprecedented global demand for virus diagnostic tests (PCR tests) has led to a severe shortage in the supply chain. One of the most important links is the virus preservation medium (VTM) used to preserve and transport samples. liquid. Due to the shortage of virus transport mediums, many countries are forced to use alternative media for virus transport mediums, such as sterile saline, liquid Amis and other inactivated preservation media. However, the gap between these alternative media and the virus transport medium is also obvious, and they are not as good as the virus transport medium in terms of functionality, stability and inhibition.   The diagnostic test of the new coronavirus is to detect the viral nucleic acid by amplifying the specific sequence of SARS-CoV-2. SARS-CoV-2 is composed of extremely unstable and easily degraded RNA. Storing virus samples in qualified environmental conditions will lead to false negative results, and the presence of microorganisms will also contaminate virus samples. For these reasons, the Centers for Disease Control and Prevention in various countries regard the correct collection and storage of the new coronavirus COVID-19 as the most important step in diagnosis.   The preservation solution for other viruses has been optimized for decades. It was originally designed to preserve the viability of the culture, and later used for nucleic acid-based tests. It is a buffer solution, virus lysate, virus nucleic acid preservation agent, and antibacterial agent. The harmful mixture can protect the virus while eliminating the contaminating microbial flora that may interfere with the test. The general ingredients are: EDTA chelating agent, guanidine salt, anionic surfactant and cationic surfactant.   Virus transport medium is divided into two types: inactivated preservation solution and non-inactivation preservation solution. The inactivation preservation solution can inactivate the collected virus, while ensuring the integrity of the viral nucleic acid in the sample, and the collected virus sample can be Transport and long-term storage under normal temperature conditions; the non-inactivated storage solution can keep the virus active and will not inhibit subsequent nucleic acid amplification, but it needs to be stored in cold storage and tested as soon as possible.   Our company can provide inactivated and non-inactivated virus preservation liquid products with excellent quality and reasonable price. For more information, please contact us: Hubei Xindesheng Material Technology Co., Ltd. hosmakesmehappy@outlook.com

Acridine ester, a yellow powder, CAS No.: 194357-64-7, has high quantum yield and high chemiluminescence efficiency, which is five times or more than that of luminol. The chemiluminescence process of acridine ester is rapid, with low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. It is one of the most widely used chemiluminescence markers and plays an important role in chemiluminescence immunoassay. Acridine ester Chemiluminescence immunoassay (CLIA) is a combination of highly sensitive chemiluminescence assay and highly specific immune reaction, which is used for the detection and analysis of various antigens, hapten, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. There are many kinds of chemiluminescence systems used in clinical examination, the most important are luminol and its derivatives, acridine ester, spiroamantane-1,2-dioxane and its derivatives, and electrochemiluminescence system. Among various chemiluminescence systems, acridine esters are considered to be ideal substrates. What are the unique advantages of acridine esters compared with luminol system? 1. The luminescence system of acridine ester is simple and no catalyst is needed. Under alkaline conditions, acridine ester molecules can be attacked by hydrogen peroxide to produce dioxane, which is unstable and decomposes into CO2 and n-methylacridone in electronic excited state. When it returns to the ground state, it emits light with a wavelength of 430nm without catalyst, and the luminescence system is simple. 2. Acridine ester has high luminous efficiency and intensity. Acridine ester chemiluminescence is a flash type, which has advantages over other techniques in the field of chemiluminescence immunoassay. The intensity of the chemiluminescence reaches the maximum after 0.4 s, the half-life is 0.9 s, and the luminescence basically ends within 2 s, which can realize rapid detection. Acridine ester has high quantum yield and high chemiluminescence efficiency, It's usually five times or more than luminol. 3. Acridine ester luminescence system has few interference factors, low background and high signal-to-noise ratio. The characteristic of acridine esters from the perspective of luminescence mechanism is that the non luminescent substituents attached to the acridine ring are separated from the acridine ring before the formation of electron excited state intermediates, that is, the non luminescent part is separated from the luminescent part, so the luminescence efficiency is not affected by the substituent structure. In addition, acridine ester has small molecular weight, easy to bind with protein through chemical bond (i.e. labeling), little influence on the conformation of antibody after conjugation (thus ensuring its good reactivity in subsequent immune detection), and good stability of conjugates. Desheng has six kinds of acridine ester products with different groups: acridine ester dmae-nhs, acridine ester nsp-dmae-nhs, acridine salt nsp-sa-nhs, acridine salt nsp-sa-nhs, acridine hydrazide nsp-sa-adh, acridine ester me-dmae-nhs. Desheng is an old chemical reagent company specializing in the R & D and production of blood test reagents. Up to now, it has 14 years of R & D and production experience. Luminescent reagent substrate has always been the top priority of the company's research. Acridine ester series has high luminous efficiency and belongs to direct luminescence. Need acridine ester series of business friends can directly contact 18971041571 (wechat number), looking forward to your call!

Carbomer 980 is a commonly used carbomer material. Carbomer is a high molecular polymer of acrylic acid allyl sucrose or pentaerythritol allyl ether. It is usually loose white micro acidic powder. It can produce high efficiency thickening under low dosage, thus producing wide viscosity range and rheological properties of emulsion, cream, gel and transdermal preparation. The properties of different carbomer are slightly different, and different models represent different viscosities, which are called short rheological or long rheological. For example, Carbomer 940 has short rheological properties, and its viscosity reaches 63000 MPa. S at 0.5%, which is suitable for the application For high viscosity products, Carbopol 941 has long rheological properties, reaching a viscosity of 7500mpa. S at 0.5% Suitable for low viscosity products, Carbomer's corresponding models are ion resistant and ion resistant. Carbomer 980 has good thickening property, high transparency, high viscosity and strong suspension ability. Carbomer 980 is widely used in daily chemical products, such as skin care emulsion, cream, transparent gel products, transparent skin care gel, hair styling gel, shampoo, shower gel and disinfectant free gel. Dissolution method in water or homogenizer: First, add carbomer into deionized water according to the actual production requirements to dissolve it without stirring. After the carbomer absorbs water naturally, take it as the criterion that there is no white powder on the surface and no white agglomerate in the solution. Stir it evenly, and then add neutralizing agent (carbomer: neutralizing agent = 1:1) 1. Kapo 5% neutralizing agent solution = 1.4.5) adjust the pH value at about 7 to reach thickening state. Use a round tool or a disperser and stir evenly at low speed. By adding homogenizer, the white group can not be seen in the actual production proportion, and neutralizer is added to form the gel, then the vacuum emulsification pot is used to remove the air from the gel. matters needing attention: 1. If the solution is dissolved by common methods, Carbomer can not be stirred in water, while stirring, Carbomer will form a white water ball, and bubbles are difficult to eliminate after neutralization; 2. After carbomer neutralization, long time stirring or high shear stirring will cause viscosity loss; 3. The existence of electrolyte will reduce the thickening efficiency of kapok resin; 4. Long term UV irradiation can reduce the viscosity of kapok resin. Desheng Technology specializes in acrylic series of blood vessel separation adhesive, Carbomer 980, caps, mops and other surfactants and biological buffers. Customers are welcome to consult.

The full name of Tris is tris(hydroxymethyl)aminomethane, which can also be called tromethamine, CAS77-86-1. Tris (Tris) is often used as a biological buffer, and TAE and TBE buffers (used for the dissolution of nucleic acids) commonly used in biochemical experiments are required. What other applications does it have besides this? Let's take a look next. In the field of medicine, Tris is not only an important pharmaceutical intermediate, it is also a kind of drug itself, suitable for metabolic acidemia, respiratory acidemia and other diseases, especially in hyperuricemia. In terms of treatment, the effect is relatively satisfactory. Hyperuricemia is divided into two categories: primary hyperuricemia and secondary hyperuricemia, mainly due to excessive sources of uric acid or (and) insufficient excretion. Under normal circumstances, if the two fasting blood on the same day is not the same, the uric acid level of men is higher than 420μmol/L, and women's uric acid level is higher than 360μmol/L, which is hyperuricemia. According to social understanding, the age of high incidence of hyperuricemia is middle-aged and old men and postmenopausal women. In recent years, there is a trend of getting younger. The current prevention and treatment strategies for hyperuricemia include reducing intake, inhibiting endogenous synthesis, and promoting renal excretion. The promotion of renal excretion is mainly achieved by alkalizing urine and inhibiting uric acid transporters. Inorganic metal cations in urine such as Ca2+, Mg2+, NH4+, etc. can inhibit the dissolution and excretion of uric acid, and alkaline factors can promote the dissolution of uric acid, so tris and sodium bicarbonate are often used in this process. But in the function of promoting uric acid dissolution and excretion, the effect of tris is better than sodium bicarbonate. After taking sodium bicarbonate, the increased cation Na+ will further resist HCO3- alkalizing urine and blood, and promote the dissolution and excretion of uric acid. Sodium bicarbonate will even reduce the solubility of uric acid after a certain dose. However, sodium bicarbonate can be produced endogenously. There is sodium bicarbonate in the human body, and there are large fluctuations in the urine, so the concentration of sodium bicarbonate is difficult to control. If the amount of sodium bicarbonate is small, uric acid will be excreted. The effect is not good. If you take more, it may increase the deposition of uric acid in the urinary system, and the intake of sodium salt will also increase the burden on the cardiovascular and kidneys. Tris is an organic amino base. There are no inorganic cations, which can avoid or weaken the inhibitory effect of inorganic cations. In addition, Tris is a foreign substance and the body will not produce tris. As a methane substance, the concentration of tris in the body is better controlled than sodium bicarbonate in the process of medication. In summary, it can be concluded that tris is better than sodium bicarbonate in promoting the dissolution of uric acid. Desheng has been doing in-depth research on biological buffers for a long time, and can provide high-quality raw materials such as Tris, Bicine, Hepes, etc. Desheng adheres to the company philosophy of "Quality First, Technology Leading", and provides customers with high-quality products.

N-Tris(Hydroxymethyl)Methyl-3-Aminopropanesulfonic Acid (TAPS Buffer) is a zwitterionic buffer widely used in the fields of biochemistry and molecular biology, CAS number: 29915-38-6, and the pH buffer range of TAPS is 7.7 -9.1, soluble in water (25g/50ml).   In clinical diagnosis, TAPS Buffer is used as a biological buffer in biochemical diagnostic kits. So what factors can affect the yield and purity of TAPS Buffer?   The reaction time, different alcohol solvents and the recycling of reaction solvents have a certain degree of influence on various types of biological buffers, and the same is true for TAPS Buffer. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, and the yield will decrease slightly; for the investigated ethanol, n-propanol, isopropanol and n-butanol reaction solvents, n-butanol As the reaction solvent, the yield and purity of TAPS are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS Buffer.   TAPS Buffer reagent TAPS Buffer is a commonly used buffer system in DNA screening systems, and also as a buffer component of RNA samples. It is suitable for electron transport and phosphorylation studies of chloroplast thin-layer preparations, and protects oxygenated hemoglobin from oxidation during freeze-drying. Methemoglobin can also be used as a background electrolyte for protein microanalysis in capillary zone electrophoresis.   The quality and service of TAPS Buffer produced and developed by Desheng are guaranteed. The manufacturer provides stable supply and can provide one-stop purchase of raw materials, saving your purchase time and cost. Desheng owns biological Buffer TAPS Buffer has complete qualifications in all aspects, including test reports and so on.