China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The detection of the new coronavirus is mainly through PCR amplification of viral nucleic acid. However, in addition to nucleic acid detection, various research and development institutions have also successively developed methods for virus antibody detection and virus specific protein detection. Recently, Shenzhen University has successfully developed a chemiluminescence new crown detection kit, and the test results can be obtained in 22 minutes!   On the evening of February 10, the world's first single-person chemiluminescence detection kit for novel coronavirus IgM and IgG antibodies jointly developed by Shenzhen University and multiple scientific research institutions announced its success. Chemiluminescence new crown detection kit results in 22 minutes   According to the news, a single copy of the new coronavirus IgM and IgG antibody chemiluminescence detection kit has completed the detection of 30 new coronavirus pneumonia patients in the local hospital. Chemiluminescence antibody detection is different from virus nucleic acid detection. This kit detects the new coronavirus-specific IgM/IgG antibody in the blood of the infected person, and can complete the rapid diagnosis of new coronavirus infection in 22 minutes.   The test kit developed this time is to detect the antibodies produced by the patient's immune response to the infected virus, not the virus itself (such as viral nucleic acid). This also avoids the risk of secondary infection caused by virus samples for the inspectors and the inspection environment.   Viral nucleic acid detection: Viral nucleic acid detection is to place a virus sample in a sampling tube with a virus transport media, and transfer it to a nucleic acid testing laboratory to perform RNA reverse transcription polymerase chain reaction RT-PCR for detection. Whether the preservation solution for processing virus samples is inactivated or non-inactivated, the nucleic acid RNA of the virus can be retained, and the object of detection is RNA.   Virus antibody detection: The object of antibody testing is not a virus sample, but a blood sample (or other body fluid) of the subject. The sampling method is through blood collection tubes or other methods. This is the use of the human body’s immune response mechanism. After a person is infected with the new coronavirus, specific antibodies will be produced in the body. As long as the specific antibodies are detected, it can indicate that they have been infected with the new coronavirus.   In the process of fighting against the common enemy of mankind, the new crown, various groups and individuals in related fields spare no effort. Desheng provides relevant products for the market including virus preservation solutions, chemiluminescence reagents, buffers, etc., to fully assist in the research and development of new crown detection technologies.

Carbomer has a wide range of uses, including: home care, industrial and detergent, personal care products / cosmetics, pharmaceutical excipients, solid fuel adhesives and alkaline batteries. Carbopol 980 is a white loose powder, it has good thickening performance, high transparency, high viscosity, strong suspension ability, short rheological Carbopol of cosolvent system, and excellent suspension stability. The main characteristics are swelling and slightly acidic.   Carbomer white loose powder   From these aspects, we can see what kind of existence it is in our life. But for such a widely used raw material, most of our country depends on foreign imports. Originally, the balance has been maintained in this way, but something unexpected happened. An epidemic at the end of the year not only disrupted our lives, but also affected our industry - carbomer is out of stock, to be exact, the products produced by carbomer are out of stock. How can we produce products without raw materials? It's hard to cook without rice! The Internet and the phone are full of people asking about carbomer raw materials.   Our Desheng company stepped forward at such an emergency. Since returning to work, we have increased manpower and material resources, and worked overtime every day to produce the raw material of carbomer. However, there are many problems. First of all, the equipment is not perfect. The company has invested a lot of money to introduce new equipment, but it has to be installed in the state of shutdown. The second site is not enough, the company leaders regardless of hard work, go all the way to find a suitable production site. The third is the shortage of manpower. The company has stepped up its recruitment efforts to recruit people with relevant work experience during this period and ensure the smooth progress of the production period. This is only the problem encountered in the early stage, and there are countless problems encountered in the follow-up. But Desheng did not give up, even in such difficult conditions, in the trust of customers, we still insist. Huangtian pays tribute to those who want to succeed in the R & D of capom of Desheng. But at present, it can only be produced in small quantities. Please trust our customers all the time. Don't worry, the victory is not far away from us. We Desheng will also adhere to our tenet: morality oriented, honesty first, quality first, technology leading.   Our service philosophy: the problems that belong to us, we do not hesitate to undertake; the problems that do not belong to us, we try our best to help customers solve; the problems that can not distinguish responsibilities, belong to us. With such a tenet and service concept, why don't we worry about big things.

We often see on TV that when the police find evidence of crime, the ground is clean. How do they know where there is blood? And through what to test the bloodstain? We have to mention our little detective Lumino. Luminosity of Luminol Luminol, also known as luminol, is a kind of chemiluminescent molecule. It is easily soluble in lye, soluble in dilute acid, almost insoluble in water, and hardly soluble in alcohol. The suitable fluorescence wavelength was 425nm (chemiluminescence was detected in 60 mm K2S2O8, 100 mm K2CO3, pH 11.5). In the presence of hydrogen peroxide, it can be transformed into excited state aminophthalic acid, which emits strong fluorescence. It is a kind of blood that can not be observed by naked eyes at the scene of crime, and can show a very small amount of blood form (occult blood reaction). The chemical name is 5-amino-benzoylhydrazide.   At room temperature is a blue crystal or pale yellow powder, is a relatively stable synthetic organic compounds. The chemical formula is c8h7n3o2. At the same time, luminol is a kind of strong acid, which can stimulate eyes, skin and respiratory tract. Hemoglobin contains iron, which can catalyze the decomposition of hydrogen peroxide, turning hydrogen peroxide into water and monooxygen, which then oxidizes luminol to make it glow. Therefore, luminol is widely used in criminal investigation, bioengineering, chemical tracing and other fields. In forensic medicine, luminol reagent uses the reagent to identify blood. Even if the blood stains are wiped, the heme in the blood will still remain. When luminol reagent is sprayed on the heme, it will oxidize with reactive oxygen species and release blue purple fluorescence. It is an organic substance used to identify blood. In biology, luminol is used to detect the presence of copper, iron and other substances in cells.   But it also has some disadvantages 1. Luminol fluoresces in the presence of iron, ferroalloys, horseradish or some bleaching agents. So if the crime scene is completely bleached, luminol's fluorescence will strongly mask the presence of any blood stains.   2. Luminol can detect a small amount of blood in animal blood and urine, so if there is urine or animal blood in the room to be tested, the test results will be biased.   3. Luminol reacts with excreta and emits the same light as blood.   4. Luminol may interfere with other tests, but luminol does not interfere with DNA extraction.   5. Luminol needs to be used in a dark environment, otherwise the fluorescence is difficult to identify.   6. Luminol luminescent time is limited, we should seize the time to take photos and record.   How can we avoid interference 1、 Let the site dry for a few days, the interference of bleach will disappear, and the bloodstain can make luminol glow even after many years.   2、 Use a compound that can inhibit the interference of hypochlorite. It's obvious that antioxidants should not be used because they will inhibit the reaction of blood stains with luminol. According to the chemical structure of hypochlorous acid, such as the chlorine atom it contains, suitable inhibitors have been found. The safe way is to use luminol luminescence method to detect the suspected blood substance, and then use other methods to determine that it is indeed blood.   Moreover, it was found that after the bloodstain was treated with luminol, the DNA contained in it was not destroyed, and it could be extracted for identification. Through the above, it is not difficult to guess how those bloodstains were detected.   Since its establishment in 2005, Desheng company has been committed to the development and production of luminescent reagents, blood collection additives, biological buffers, etc., with rich experience.

Now more and more women pay attention to skin care, and even more and more men begin to pay attention, because a good skin will give people a clean and comfortable impression. On the market, mild hydrated and non irritating cosmetics are more and more popular with the public. The advertising slogan of major brands of cosmetics is "add safely and non irritating", which makes the majority of consumers and manufacturers pay attention to the added ingredients of cosmetics, and HEPES and Tris become popular additives.   HEPES (7365-45-9) and tris (77-86-1) are the ingredients with EWG green certification, which are safe and mild. The certification level of tromethamine is 1-2, and that of HEPES is 1. This article will introduce the role of HEPES and Tris in cosmetics.   HEPES and Tris are important buffers widely used. Tris is weakly alkaline, PKA is 8.06 at 25 ℃, pH buffer range: 7.0-9.0, widely used in biochemistry, molecular biology, coatings and other fields.   HEPES, 4-hydroxyethyl piperazine ethanesulfonic acid, is a kind of zwitterionic buffer, belonging to good's buffer, with pH buffer range of 6.8-8.2. HEPES buffer is often used in the research of organelles and pH sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits.   As a component of cosmetics, HEPES and Tris have different functions and advantages. The most common function of Tris in cosmetics is to adjust pH value (pH value). It can also be used as a neutralizing agent to neutralize Thickener (carbomer), so as to reduce the sticky feeling, improve the respiratory efficiency of skin cells, and prevent pore blockage.   In addition, Tris can be used as an odor regulator in cosmetics containing amine salts to regulate the odor of amine produced by rubbing when applying cosmetics, so as to avoid the release of any residual or persistent odor.   HEPES has the advantages of safety to human body, non-toxic, good physiological compatibility and stable chemical performance. From the perspective of EWG certification level, HEPES is safer than Tris. HEPES can stabilize the pH range of skin care products for a long time, better maintain the activity of microbial fermentation products extract, and make the anti-aging effect of products last for a long time, so it is often used as a stabilizer and activator in cosmetics.   HEPES is also a common penetration enhancer, which can promote the transdermal absorption of various functional components in cosmetics. Compared with azone penetration enhancers, HEPES has the characteristics of short onset time, less dosage and high penetration rate. In addition, in some famous cosmetics such as L'Oreal and Lancome, HEPES is mainly used as a peeling agent to soften cutin and promote the metabolism of skin cells.   EWG certification has become an important standard for people to choose safe cosmetics. EWG safety level is 1-10, of which 0-2 is green safety level, 3-6 is yellow, representing medium hazard, 7-9 is red, representing high hazard. The EWG certification of HEPES and Tris belong to the green level, reaching the international standard, meeting the people's demand for "safe and natural" cosmetic ingredients.   Desheng has more than ten years of experience in the production and development of biological buffers, blood collection additives and in vitro diagnostic reagents, and insists on providing high-quality products for customers. HEPES and Tris are popular products of the company. Can also provide customers with neutralizing thickener carbomer.

Recently, a friend just bought a new house and was worried about the decoration. Because there are old people and children at home, we are very careful in choosing materials. Later, when I heard about this, I directly recommended him to buy water-based coatings containing caps, because my own company developed and produced caps products, from which I learned its advantages in coatings. In addition, people's awareness of health and environmental protection is becoming more and more intense. This trend forces industry giants to compete to develop new environmental protection coatings. Caps (3 - (cyclohexylamino) - 1-propane sulfonic acid) stands out in this competition and becomes the leader of new environmental protection coatings. Let me talk about why we should choose water-based coatings with caps. What role does caps play?   Caps, 3 - (cyclohexylamine) - 1-propanesulfonic acid, cas1135-40-6, is a member of good's buffer. Caps has a wide range of uses. The properties of 3-cyclohexylaminopropane sulfonic acid (CAPS) are relatively stable. The pKa value is 10.4 at 25 ° C, and the pH buffer range is 9.7-11.1. In the 1960s, a kind of polyurethane coating began to emerge. In most application fields, water dispersible polyisocyanate was modified by polyether to achieve non-ionic hydrophilic. Although this kind of hydrophilic polyisocyanate has been widely recognized in the market, it still has some defects, such as high viscosity. In the process of use, it needs to apply a large shear force to blend it into the water medium evenly. Especially when it is used as the crosslinking agent of waterborne two-component polyurethane coatings, it needs a high polyether content to ensure sufficient dispersion, which is very important It results in a long drying time and gives the coating lasting hydrophilicity.   In order to avoid these shortcomings, researchers have tried to prepare water dispersible polyisocyanates by adding ionic groups to hydrophilic modification, including carboxyl groups, sulfuric acid groups, hydroxyl groups, and hydroxysulfonic acid groups. However, there are still certain defects, such as carboxyl modified polymers, which are easy to gel, and the color of products modified by hydroxysulfonic acid is obviously yellow. Later, Bayer company reported the caps modified polyisocyanate in its patent cn1429240a. It was found that the caps modified polyisocyanate could be finely dispersed in water and the product was stable in storage. Aliphatic polyisocyanates reacted with caps under mild conditions and in the presence of tertiary amine neutralizer to obtain Sulfonate Modified polyisocyanate crosslinker   CAPS modified polyisocyanate has good storage stability. Even if it contains less sulfonate base group, it can also get very good emulsion in water. There are many market-oriented products, which can be used in a variety of environmentally friendly high-quality waterborne two-component polyurethane coatings. These coatings can fully achieve the performance of solvent based coatings in terms of drying, curing and chemical resistance. If the new regulations require further reduction of VOC, the amount of these crosslinking agents will increase greatly in the future, because compared with solvent based coatings, they will not lead to the deterioration of film quality. By using hydrophilic modified polyisocyanate and simple manual stirring, the uniform mixture of base material and crosslinking agent without cosolvent can be obtained.   As mentioned above, the water-based coatings market led by caps will seize the market share at a rapid speed. The application of caps in the field of biochemical industry is also rising step by step with the rapid development of the medical industry.   Caps developed and produced by Desheng company is not only used in new coatings, but also used in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits. Customers in need are welcome to contact us.

Under the influence of the epidemic, a large number of Virus Transport Media also appeared in the public view. But our understanding of it is only limited to know that it is collecting virus for detection, but what is its own ability? Why is it also called inactivated Virus Transport Media? We can only understand according to the literal meaning, that is to kill the live virus and do research after preservation. But is it really that simple? It's just the idea of a lot of people who don't understand. First, let's see why it's called inactivation?   What is inactivation? Inactivation is a method of killing viruses and bacteria by physical or chemical means without damaging their useful antigens.   Inactivated Virus Transport Media: it is a colorless transparent liquid, suitable for viruses, New Coronavirus, influenza, avian influenza, hand foot and mouth urticaria and other viruses. It destroys the structure of virus protein, and the protein has no physiological activity, so it loses the ability of infection, pathogenicity and reproduction. However, conventional inactivation does not affect the primary structure of virus protein, which means that the sequence of virus protein has not changed.   The inactivated samples can be matched with the corresponding New Coronavirus RNA extraction kit, M32/M96 nucleic acid extractor and other rapid extraction of virus nucleic acid, and New Coronavirus PCR detection kit to achieve rapid detection, specificity sensitivity is not affected. Applicable kit methods: fluorescent PCR, combined probe anchored polymerization sequencing, isothermal amplification chip, magnetic particle chemiluminescence, colloidal gold.   So the inactivated virus has antigenicity, but loses infectivity. This is actually how many vaccines are made.   The inactivated virus lost its infective activity, but still had fusion activity. It is an inducer for cell fusion in animal cell engineering. Inactivated Virus Transport Media has the following advantages: 1. Inactivate the virus to avoid cross infection of centralized sampling 2. Inactivate the virus and reduce the difficulty of preservation and transportation 3. The samples lose their infectivity and protect the testing personnel 4. It is widely used in PCR laboratory   In addition to the above inactivated Virus Transport Media, Desheng company also developed and produced non inactivated Virus Transport Media, which contains Hanks solution base, gentamicin, fungal antibiotics, BSA (V), cryoprotectants, biological buffers and amino acids. Non inactivated Virus Transport Media is usually used for the collection and transportation of clinical influenza, avian influenza (such as h7n9), hand foot mouth disease, measles and other virus samples, as well as Mycoplasma, Ureaplasma, chlamydia and other samples.

As a new Trinder's reagent, Maos reagent plays an important role in it. Its Chinese name is n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3,5-dimethylaniline sodium salt monohydrate, which is a white powder and soluble in water, Sensitive to light and humidity, CAS No.: 82692-97-5, Maos product produced by Desheng, purity ≥ 99%, good water solubility, stable process, can ensure the appearance of pure white crystal powder.   The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic detection and biochemical tests. In the colorimetric determination of hydrogen peroxide activity, it has several advantages over conventional chromogenic reagents. The new Trinder's reagent is stable enough to be used in both solution and test line detection system. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent formed very stable purple or blue dyes in the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolylsulfonylhydrazone (MBTH). The molar absorptivity of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA; however, 4-AA solution is more stable than MBTH solution. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration.   Therefore, the amount of substrate can be determined by the color reaction of oxidative coupling reaction. Glucose, alcohol, acyl coenzyme A and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA. There are 10 kinds of new Trinder's reagents, and the application of Maos is also very important. For specific substrates, it is necessary to test different kinds of new Trinder's reagents to develop the best detection system.   The new Trinder's reagent Maos is one of Desheng's main products. Desheng's products have passed the strict quality inspection and passed the national ISO9001 quality management system certification. Not only the quality is good, but our price is also very favorable compared with many other manufacturers. If you have the intention to purchase, we can provide you with a small sample for trial, use well and then buy back. The specific situation is as follows Please contact us

Chemical agents or substances that can prevent blood coagulation are called anticoagulants or anticoagulants, such as natural anticoagulants (heparin, hirudin, etc.), Ca2 + chelating agents (sodium citrate, potassium fluoride). Commonly used anticoagulants are heparin, EDTA, citrate, oxalate, etc. in practical application, we should choose according to different needs, in order to obtain the ideal effect. Their application characteristics are summarized as follows:   1、 Heparin Heparin is the preferred anticoagulant for the detection of blood chemical composition. Heparin is a kind of mucopolysaccharide containing sulfate group, which is a kind of dispersive phase material with an average molecular weight of 15000. Its anticoagulant mechanism is mainly through the combination with antithrombin III to cause the change of antithrombin III configuration and accelerate the formation of thrombin thrombin complex to produce anticoagulant effect.   In addition, heparin can inhibit thrombin by plasma cofactor (heparin cofactor II). The commonly used heparin anticoagulants are sodium, potassium, lithium and ammonium salts of heparin, among which heparin lithium is the best, but its price is more expensive. Sodium and potassium salts will increase the content of sodium and potassium in blood, and ammonium salts will increase the content of urea nitrogen. The dosage of heparin anticoagulation is usually 10.0-12.5 IU / ml blood.   Heparin has less interference on blood components, does not affect the volume of red blood cells, and does not cause hemolysis. It is suitable for red blood cell permeability test, blood gas, plasma permeability, hematocrit and ordinary biochemical determination. However, heparin has antithrombin effect and is not suitable for hemagglutination test.   In addition, excessive heparin can cause leukocyte aggregation and thrombocytopenia, so it is not suitable for leukocyte classification and platelet count, let alone hemostasis test. In addition, heparin anticoagulant can not be used to make blood smear, because Wright staining appears dark blue background, which affects the production reduction of microscope. Heparin anticoagulant should be used in a short period of time, otherwise the blood can coagulate if it is placed too long. 2、 Ethylenediamine tetraacetate (EDTA salt) EDTA can combine with Ca2 + in blood to form a chelate, the coagulation process is blocked, and the blood can not coagulate. EDTA salts include potassium, sodium and lithium. EDTA-K2 is recommended by the International Committee for standardization of Hematology, which has the highest solubility and the fastest anticoagulation speed. EDTA salt is usually prepared into 15% aqueous solution, adding 1.2mg EDTA per ml blood, that is, adding 0.04ml 15% EDTA per 5ml blood. EDTA salt can be dried at 100 ℃, and its anticoagulant effect remains unchanged.   The anticoagulant does not affect the count and size of white blood cells, has the least effect on the morphology of red blood cells, and can inhibit platelet aggregation, so it is suitable for general hematological detection. But if the concentration of anticoagulant is too high, the osmotic pressure will rise, which will cause cell shrinkage.   The pH of EDTA solution has a great relationship with salts, and low pH can make the cells expand. EDTA-K2 can slightly expand the volume of red blood cells, the average platelet volume is very unstable in a short time after blood collection, and tends to be stable after half an hour. EDTA-K2 can decrease Ca2 + and Mg2 +, and decrease creatine kinase and alkaline phosphatase. The optimal concentration of EDTA-K2 is 1.5mg/ml blood. If the blood is less, neutrophils will swell and disappear, platelets will swell and disintegrate, and normal platelet fragments will be produced, which makes the analysis results wrong.   Because EDTA can inhibit or interfere with the polymerization of fibrin monomer during fibrin clot formation, it is not suitable for the detection of blood coagulation and platelet function, nor for the determination of calcium, potassium, sodium and nitrogenous substances. In addition, EDTA can affect the activity of some enzymes and inhibit lupus erythematosus factor, so it is not suitable for making blood smear for histochemical staining and examination of lupus erythematosus cells. 3、 Citrate Citrate is mainly sodium citrate. Its anticoagulant principle is that it can combine with Ca2 + in blood to form chelate, which makes Ca2 + lose coagulation function, and the coagulation process is blocked, so as to prevent blood coagulation. Sodium citrate has two kinds of crystals, na3c6h5o7 · 2H2O and 2na3c6h5o7 · 11h2o. The former is usually used to make 3.8% or 3.2% aqueous solution, which is mixed with blood according to the volume of 1:9.   Most coagulation tests can be anticoagulated with sodium citrate, which is helpful to the stability of factor V and factor VIII, and has little effect on mean platelet volume and other coagulation factors, so it can be used for platelet function analysis. Sodium citrate is one of the components of blood maintenance fluid in blood transfusion. However, sodium citrate 6mg can anticoagulate 1ml blood, strong alkaline, not suitable for blood tests and biochemical tests.   4、 Oxalate Oxalate is also a commonly used anticoagulant, which has the advantage of high solubility. Its principle of action is that the dissociated oxalate after dissolution forms calcium oxalate precipitation with Ca2 + in the sample, which makes Ca2 + lose the coagulation function and blocks the coagulation process.   The commonly used oxalate anticoagulants are sodium oxalate, potassium oxalate and ammonium oxalate. The concentration of sodium oxalate is 0.1 mol / L, and the ratio of sodium oxalate to blood is 1:9. However, high concentration of K + or Na + is easy to make the blood cells dehydrate and shrink, while ammonium oxalate can make the blood cells expand. Therefore, the anticoagulant with appropriate proportion of ammonium oxalate and potassium oxalate or sodium oxalate does not affect the shape and volume of red blood cells. It is often used in blood biochemical determination, but it is not suitable for the determination of K + and Ca2 +.   Due to the formation of calcium oxalate precipitation, red blood cells will appear serrated, white blood cells will appear vacuoles, lymphocytes and monocytes will be deformed, it is not appropriate to do blood test. Oxalate can cause platelet aggregation and affect the morphology of leukocytes, so it can not be used for the differential count of leukocytes and platelets. Desheng is an old manufacturer of vascular additives. It has formed its own intellectual property rights and professional production and R & D capabilities in the aspect of vascular additives. It has provided products and raw material solutions for more than 100 domestic and foreign manufacturers.   The anticoagulant series products of blood samples include heparin sodium, heparin lithium, trisodium citrate, EDTA dipotassium, EDTA Tripotassium, potassium oxalate, etc.; the anticoagulant series products of blood samples include blood coagulant powder, blood coagulant, etc.; the pretreatment materials of blood samples include separation gel, silicide, etc. A variety of raw materials for you to choose, need to click the official website to consult our professional customer service.

CLIA was born in 1977. According to the basic principle of radioimmunoassay, chemiluminescence immunoassay was established by combining high sensitive chemiluminescence technology with high specific immune response. CLIA has the advantages of high sensitivity, strong specificity, wide linear range, simple operation and no need of expensive equipment.   CLIA is widely used in the detection of antigens, haptens and antibodies of different molecular sizes, as well as nucleic acid probes. Compared with RIA, IFA and EIA, CLIA has the advantages of non radiation, long validity period and full automation.   Chemiluminescence immunoassay consists of two systems: immunoassay and chemiluminescence analysis. Immunoassay system uses chemiluminescent substances or enzymes as markers, which are directly labeled on the antigen or antibody. After the reaction of antigen and antibody, antigen antibody immune complex is formed.   Chemiluminescence analysis system is after the end of the immune reaction, add oxidant or enzyme luminescent substrate, chemiluminescence material after oxidation by oxidant, form an excited state intermediate, will emit photons to release energy to return to the stable ground state, the luminous intensity can be detected by luminous signal measuring instrument. According to the relationship between chemiluminescent markers and luminous intensity, the content of the measured substance can be calculated by standard curve.   Chemiluminescent immunoassay (CLIA) is a kind of immunoassay method which uses chemiluminescent agent to label antibody or antigen directly. At present, luminol and acridine esters are the most common chemiluminescent agents.   1 chemiluminescence immunoassay of luminol: luminol is an oxidative reaction. Luminol can be oxidized by many oxidants in alkaline solution, among which H2O2 is the most commonly used. Due to the slow reaction rate, some enzymes or inorganic catalysts need to be added. The main enzymes are horseradish peroxidase (HRP), and the inorganic ones include O3, halogen, Fe3, Cu2, CO2 and their complexes.   In the early stage, it was mainly used for the determination of inorganic and organic biological small molecules, and the sensitivity decreased due to the decrease of the luminous intensity after labeling. It has been found that the addition of Some Phenols and their derivatives, amines and their derivatives, and phenylboronic acid derivatives can significantly enhance the luminescence of the system. The luminescence intensity can be increased by 1000 times, and the "background" luminescence is significantly reduced, and the luminescence time is also prolonged. The use of these enhancers makes chemiluminescence immunoassay widely used in protein and nucleic acid analysis.   2 acridine ester labeled chemiluminescence immunoassay: acridine ester was used in CLIA. Because of its poor thermal stability, more stable acridine ester derivatives were synthesized. Under the condition of H2O2 and oh -, acridine esters can emit light rapidly with high quantum yield. For example, the quantum yield of acridine aromatic esters can reach 0.05. When acridine esters are used as markers for immunoassay, the luminescence system is simple, rapid, and does not require the addition of catalyst. Moreover, the labeling efficiency is high and the background is low. These characteristics arouse the great interest of the majority of analysis and diagnosis workers.

Toos, n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline sodium salt, is a widely used color reagent, which is most commonly used in the new Trinder's reagent. Toos has been used in many clinical biochemical test items. This article will introduce the specific test items for which the chromogenic reagent toos can be used.   Through Trinder reaction, toos is widely used in diagnostic detection and biochemical tests. Trinder reaction is also known as "coupling end point colorimetry". The principle is that hydrogen peroxide (H2O2) produced by enzyme reaction can generate red quinoline imine compound in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD). The specific detection application of toos mainly includes blood glucose detection And liver function test, the following is a brief introduction to the detection principle of these two methods.   TOOS, the substrate of Desheng chromogen 1. Blood glucose detection project: toos is used to prepare glucose detection reagent and glycosylated albumin detection reagent in blood glucose metabolism project. According to the description of patent cn105572065a, glucose oxidase is used to catalyze the oxidation of glucose to hydrogen peroxide, and platinum bismuth nano mimetic peroxidase is used to catalyze the oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and sodium salt of n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline (toos) by hydrogen peroxide. The color of the color developing product solution is purple, and with the concentration of glucose increasing The maximum absorption wavelength of the color system is 590 nm, and the content of glucose can be obtained from the absorbance at 590 nm.   2. Routine examination items of liver function: adenosine deaminase (ADA) activity is a sensitive index reflecting liver injury, and is one of the routine examination items of liver function. The detection reagent of adenosine deaminase composed of toos, bovine serum albumin, disodium ethylenediamine tetraacetate, etc., has the advantages of good color effect, rapid response, stability and high precision.   In addition, toos is also used in the diagnostic reagents for triglyceride and other blood lipid detection items and cholesterol detection items, which has important value in clinical diagnosis. The toos chromogenic reagent produced by Desheng is not only of high purity, but also of high quality, High sensitivity, and in the absorption wavelength, color reaction intensity and fading have good performance, welcome the majority of scientific research workers and dealers colleagues to consult and purchase!

Nowadays, as long as people have a headache and brain fever, they first go to the hospital for testing to find out where the problem lies, and then prescribe the right medicine. In fact, many people only know the test items, but they don't know much about the test materials. The DAOS in the new Trinder’s reagent is a very important test material, which has the advantages of good water solubility, high sensitivity, and strong stability. Let's take a look at the past of DAOS on the road of detection. DAOS can be used for cholesterol testing When measuring total cholesterol, the cholesterol ester is firstly hydrolyzed into cholesterol and fatty acid by cholesterol esterase CHE, and then it is catalyzed and oxidized to 4-cholestenone and hydrogen peroxide by cholesterol oxidase, and then it is through DAOS and 4-AAP Coupling with hydrogen peroxide to produce a color reaction under the catalysis of POD, the concentration of total cholesterol can be determined by measuring the absorbance.   DAOS can be used for triglyceride detection When detecting triglycerides, triglycerides are hydrolyzed into glycerol and fatty acids in the presence of lipoprotein esterase (LPL); glycerol and ATP are catalyzed by glycerol kinase (GK) to generate glycerol-3-phosphate and ADP; glycerol-3- Phosphoric acid and oxygen are catalyzed by glycerol-3-phosphate oxidase (GPO) to generate dihydroxyacetone phosphate and hydrogen peroxide, and then use the color substrate to couple 4-AAP and hydrogen peroxide to produce color as in the above steps. In response, the TG concentration was measured.   DAOS can be used for high-density lipoprotein detection When detecting high-density lipoprotein, a dual-reagent reaction is used. Reagent one contains polyanion and surfactant, which selectively binds to VLDL and LDL, and inhibits COD and CEH in reagent two on VLDH-CH and LDH-CH. , Thereby selectively acting on HDL-CH, through the action of CEH-COD-POD, and then measuring the absorbance to determine the concentration of HDL, and finally the LDL concentration can be obtained by calculation.   Cautions when using DAOS: 1. Divided packaging: When taking a small amount of mg DAOS, the product needs to be divided into the quantity needed each time to reduce the number of bottle openings and prevent the product from absorbing moisture. 2. Use: When using the product, you need to take out the product from the freezer half an hour in advance and place it at room temperature. For the remaining, store the remaining DAOS in a sealed and light-proof container to avoid quality problems such as changes in color or properties of the product. 3. Preservation: Place DAOS in a 0-5 degree freezer, and record the time and batch number. 4. Transportation: Put ice cubes in the foam box for the packed products and wrap the products with foam glue. Take care to prevent the water from the ice cubes from getting the product wet (we put two more if the temperature is high, and the weather can be changed in winter. Temperature to decide)

Acridine ester (nsp-dmae-nhs), yellow powder, CAS No.: 194357-64-7, is an important chemiluminescence reagent. Its chemiluminescence quantum yield is higher than that of luminol. Moreover, the labeling conditions of acridine ester are mild, the labeling rate is high, and the labeling does not affect the separation.   In addition, the chemiluminescence process of acridine ester is rapid with low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. In addition, acridine ester chemiluminescence reagents have good stability and are easy to store.   In addition to the application in immunoassay, acridine ester can also be used to label oligonucleotide fragment probes for gene detection or microbial detection. Acridine ester compounds are suitable for labeling DNA strand to make chemiluminescence DNA probes.   Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutation, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of specific sequence of virus DNA is conducive to the control of epidemic situation. The detection of DNA sequence and base mutation in its strand is of great significance in gene screening, early diagnosis and treatment of genetic diseases, and detection of pathogenic genes.   In nucleic acid hybridization analysis, the preparation of specific and sensitive labeled probe is the key to the success of nucleic acid hybridization analysis. Acridine ester derivatives can be directly labeled on the nucleic acid probe without catalyst and the luminescence quantum yield is not affected. In addition, under certain conditions, the acridine ester labeled on the unhybrid single strand DNA is hydrolyzed and destroyed, and only the double strand formed by hybridization is protected Only acridine ester can produce chemiluminescence, and the whole hybridization process can be monitored without separation. The invention relates to a method for labeling nucleic acid with acridine ester, which comprises the following steps: (1) The 5 'and 3' ends of DNA probe were protected; (2) Acridine ester labeling: 25 mm NHS acridine ester was dissolved in DMSO, and 1 m HEPES buffer (pH = 8.0) was prepared. According to the molar ratio of nucleic acid probe: NHS acridine ester = 1:5, it was added into HEPES buffer and reacted at 37 ℃ for 1 h;   (3) Purified by HPLC. In the step (1), the protection of 5 'and 3' ends of DNA probe specifically includes: using s-ethyl trifluorothioacetate to protect 5 'end-nh 2; using s-ethyl trifluorothioacetate to protect 3' end-nh 2.   Desheng technology has been researching and developing chemiluminescence reagents for 12 years. After successful development, it has been put into the market and won the general recognition of customers. There are six different groups of acridine esters. Besides acridine esters, there are luminol and isoluminol. Acridine ester series have high luminous efficiency and belong to direct luminescence.