China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Hubei New Desheng Material Technology Co., Ltd. is a professional manufacturer of raw materials for biological buffer.The biological buffer produced by our company are stable with high purity.It can be used in common skin care products and cosmetics such as toner, essence, lotion, eye cream and face cream middle. Here are three buffers commonly used in cosmetic raw materials, 3(-hydroxymethyl)aminomethane (Tris), 4-hydroxyethylpiperazineethanesulfonic acid (HEPES),and bis(2-hydroxyethyl)idene Amino tris (hydroxymethyl) methane (Bis-Tris). TRIS base 3(-Hydroxymethyl)aminomethane (TRIS), also known as tromethamine, is a zwitterionic biological buffer. In the PH range of 7.2-9.0, TRIS has the best buffering ability, and can maintain the PH value of the solution for a period of time at 4.75-5.75 that is just suitable for human skin. Therefore, TRIS buffers are often used in emulsifiers, thickeners, moisturizers and other formulations to adjust and stabilize the acidity and alkalinity of products. Because TRIS buffers can reduce or suppress products’ odor, they can be used in the formulation of sunscreens, leave-in nail polishes, eye makeup and lotions. HEPES Like TRIS, 4-Hydroxyethylpiperazineethanesulfonic acid (HEPES) is also a zwitterionic buffer with a suitable pH range of 6.8-8.2. HEPES buffer is often used to adjust the PH value of cosmetic products, stabilize it in a weakly acidic state that is suitable for human skin.It does not affect the efficacy of other ingredients, and even promotes the transdermal absorption of its functional ingredients. The concentration of the buffer affects its buffering capability,a certain concentration of HEPES can be used in exfoliating products. Bis-Tris Bis (2-hydroxyethyl) imino tris (hydroxymethyl) methane (Bis-Tris) is a weakly acidic buffer containing trace ionized hydrogen ions. In raw materials of cosmetics, Bis-Tris can adjust the PH value of the product and keep it stable in a suitable acid state. Bis-tris buffer has excellent decontamination, dispersing, emulsifying, anti-static ability, and high ultraviolet absorption ability, so it is often used to replace maleic acid in some sunscreens. The TRIS base, HEPES and BIS-TRIS produced by our company are white crystalline powder with excellent properties.The purity can reach more than 99%. Professional R&D team can customize indicators according to your requirements, welcome to consult.

In biochemical tests, it is often seen that the serum in the upper layer of the blood collection tube with serum separating gel is red after being centrifuged. Which is the rupture of blood cells in the test tube and the escape of hemoglobin. Outside the human body, if the RBC (red blood cells) are in a solution with too low osmotic pressure, water enters the red blood cells, causing swelling and rupture, resulting in hemolysis. Hemolysis in the human body is mainly due to the inherent defects of red blood cells, or due to the presence of auto antibodies, chemicals, snake venoms and other factors in plasma, causing excessive destruction of RBC. So why does hemolysis occur in a test tube containing serum separation gel?   First, let's understand the principle of separating serum and blood clot with separating gel. After the blood sample is put into the test tube, under the action of thrombin factor, fibrinogen is converted into insoluble fibrin filaments which encircled the blood cells to form blood clots and precipitate a part of serum,that is natural coagulate which is difficult to fully coagulate.With characteristic of thixotropy , the separating gel in the tube becomes liquid and flows under the action of centrifugal force. Due to the specific gravity, the blood clot with higher specific gravity settles in the bottom, the lower serum is in the upper layer, and the separating gel is in the middle. After the centrifugal force disappears, the separating gel becomes gel and completely separates the blood clot from the serum. Separating gel is inert, it does not react with anything in the chemical reaction. So it does not change the products of the reaction, nor does it destroy the hemoglobin in the cells. From this point, serum separation gel is not the cause of the destruction of blood cells.   What Should Be Notice When Using Serum Separation Gel 1. Disinfect with iodine, the alcohol in the iodine is not dry and enters the blood collection tube to cause pollution and destroy red blood cells; 2. Blood collection, if the blood collection volume is insufficient and the pressure is too large, the RBC in the tube will rupture, which cause hemolysis; 3. Transfer the sample to the blood collection tube with a blood collection needle,the blood cells were damaged due to the action of pressure; 4. Invert and mix the blood collection tube about 5 times. If the force is too large, the blood cells will rupture; 5. Centrifuge the sample blood before it has fully reached the time of coagulation. Hubei New Desheng Material Co.,ltd is specialized in serum separation gel, Stable delivery and guaranteed quality.Welcome to inquiry.

Vacuum lancets are mainly used for blood collection and preservation. It is composed of vacuum blood collection tube,needle (including straight needle and scalp blood collection needle), needle holder and blood collection tube additives, which are used to meet multiple comprehensive blood tests in clinical practice. Different blood collection tube additives are used in different biochemical and immune and other clinical medical tests. According to the different additives of blood collection tubes, vacuum blood collection tubes are generally divided into three types, anti-coagulant,anticoagulant,and serum separation gel blood collection tubes.   Anti-anticoagulant Blood Collection Tubes Different anticoagulants are added to the blood collection tubes for specific test items .An anticoagulant tube filled with heparin sodium is used for blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, and red blood cell fragility test.For plasma separation and testing, electrolyte testing,then blood collection tube with Desheng’s serum separation gel and heparin Lithium.EDTA anticoagulant tubes are used for general hematology tests.For blood sugar testing,anticoagulant tubes containing potassium oxalate or sodium fluoride are used.   Coagulant blood collection tubes For coagulant blood collection tubes,silicone oil sprayed over the wall to prevent hanging,and coagulant reagent is added.Coagulant reagent can activate fibrin protease which turn soluble fibrin into insoluble fibrin aggregates, and then form stable fibrin clots. Rapid blood coagulation with physical reaction,no need other heating facilities.After the blood collection is completed,put the tubes straight for 15 minutes and then centrifuge under 16℃. Which can make high-purity serum can be obtained for general emergency biochemistry.   Blood collection tubes with serum separating gel It’s a kind of blood collection tubes containing serum separating gel and coagulant. The tube wall is siliconized and coated with coagulant to accelerate blood coagulation and shorten the test time. The separation gel in the tube has a good affinity with the PET tube and plays an isolation role. Generally, even on a common centrifuge, the separation gel can completely separate and accumulate the liquid components (serum) and solid components (blood cells) in the blood. And form a barrier in the test tube. No oil droplets are produced in the serum after centrifugal to clog the machine.Blood collection tubes with serum separation gel are mainly used for serum biochemistry (liver function, kidney function, myocardial enzyme, amylase, etc.), electrolytes (serum potassium, sodium, chloride, calcium, phosphorus, etc.), thyroid function, drug testing, AIDS testing, tumor markers, serum immunity study.   Hubei New Desheng Material Technology Co., Ltd is a professional manufacturer of blood collection tube additives. After 17 years of research and production, 13 kinds of blood collection tube additives produced, including 8 anticoagulants: heparin sodium, heparin lithium, EDTA K2, EDTA K3,EDTA Na2, Potassium Oxalate, Trisodium Citrate, Sodium Fluoride; 2 coagulants: coagulation reagent, high-efficiency coagulation powder; 3 adjuvants: anti-irradiation separation gel,silicon oil, water-soluble silicide reagent. Product quality is guaranteed.Welcome to consult  http://www.vacutaineradditives.com/

Desheng-Your First Choice of VTM       Usually the virus test is to check whether the nucleic acid of the virus is exist in the throat swab sample. Viruses are a single substance containing only nucleic acids and proteins. The proteins and nucleic acids are decomposed when the virus leaves the host cell,making it impossible to correctly determine the virus in the collected sample during detection. The viral transport media(VTM) preservation solution can be used to immerse the virus sample in the sampling tubes, inactivate the virus protein and release the virus nucleic acid for subsequent detection.       With the outbreak of the covid-19, a large number of VTM required to ensure the effectiveness of nucleic acid tests. Under the leadership of the company's leaders, Desheng's researchers developed a unique inactivated VTM in March 2020, contributing their own strength to fight against the covid-19, which also reflects that Desheng's company have courage to assume social responsibility in front of disaster. There are two types of VTM produced by Desheng, inactivated and non-inactivated. The inactivated viral transport media contains biological buffers and lysis salts.After lysing and releasing nucleic acids,testing with the virus PCR detection kit to achieve rapid detection.So as to determine whether the sample contains nucleic acid of virus characteristic , that is, whether it is infected with the virus.       The components of the non-inactivated VTM include Hank's buffer, which can adjust the pH value of the preservation solution and ensure a neutral environment for virus survival.Combined antibiotics with antibacterial and antifungal effects Bacterial protein stabilizer bovine serum albumin, which provide essential amino acids for viruses.Cryoprotectants to protect cells from solution and ice crystal damage.And glycerol to resist changes in temperature.       With the continuous advancement of technology, the quality of VTM produced by Desheng is stable, and the production capacity can reach 50 tons per day, which can meet the demand for covid-19 detection. Welcome friends to consult. The virus is ruthless, but there is love in the world. Let’s fight against the epidemic together and tide over the difficulties together. We hope the epidemic will end as soon as possible.

Luminol, also known as 3-Aminophthalohydrazide, a kind of white powder at room temperature. It is a strong acid after being configured into a solution, which has a certain stimulating effect on eyes, skin and respiratory tract. When the luminescent ammonia base solution is treated with an oxidizing agent, the released energy exists in the form of photons, and the wavelength acts as a visible blue light. Therefore, when luminol is treated with nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants, it will emit blue light. Based on this principle, luminol is the most common chemiluminescent substance. The following will introduce you the applications of Luminol: 1.Luminol can be used for Arabidopsis thaliana immune-related reactions in Luminol peroxidase-based analysis. 2. As a chemiluminescent substrate Luminol Can be used to detect opsonization and phagocytosis function. 3. As a biodetector. It can be used to detect polymorphonuclear leukocyte (PMNL) response in patients with myeloperoxidase deficiency (MPO). 4. Metal cation analysis, using simple and inexpensive instruments with luminol, can detect metal ions at the part-per-billion level. 5. For glucocorticoid detection and analysis. 6.In biology, luminol is used to detect the presence of copper, iron and cyanide in cells. Usually, a mixed aqueous solution of hydrogen peroxide and a hydroxide base is used as stimulated agent. 7.Testing blood in forensic medicine. Iron in hemoglobin in the Blood catalyze the decomposition of hydrogen peroxide, turning it into water and monooxygen. Monooxygen reoxidizes luminol to make it glow. When detecting blood stains, luminol reacts with heme emitting blue-green fluorescence. This detection method is extremely sensitive. The investigator sprays a solution of luminol and an activator in the area to be investigated, and iron in the blood immediately catalyzes the luminol reaction, which emit a blue light. After the bloodstain is treated with luminol, the DNA of the genetic material it contains has not been destroyed, and can be extracted for identification.When using luminol to detect blood stains, the standard forensic solution containing alkaline luminol and sodium perborate may react with industrial substances which contains copper and living substances such as pesticides, glues, parsnips, radishes to emit light . That can interfere with the detection of hemoglobin in the blood. In this case, the light-emitting substances can be distinguished by the wavelength of the light. Hubei New Desheng Material Technology Co., Ltd. is a professional manufacturer of chemiluminescence reagent Luminol, which can be stored in the dark at 2-8°C for up to 12 months. After 17 years of R&D and production, our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.

Potential cross-contamination of additives between primary blood collection tubes is a common problem during sample collection, and contaminated additives have a significant impact on blood test results. Citrate blood contamination with varying amounts of dipotassium ethylenediaminetetraacetate (K2 EDTA blood) had significant effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrinogen.Fifteen samples containing healthy blood were collected into 0.109m vessels witch 4pcs with citrate and 1 pc with K2 EDTA. Combine four citrate tubes and divide into five equal portions. Then add whole blood sample with K2 EDTA in scalar amounts to autologous citrated blood aliquots to obtain 0% to 43% polluted K2 EDTA .Resulting statistically and clinically significant with prolongation of APTT and PT was observed between 29% and 43% K2 EDTA contamination,while fibrinogen values reduced at 43% K2 EDTA contamination.It indicates that contamination of citrated blood with up to 29% of K2 EDTA blood can cause significant bias in routine coagulation test results.This has serious implications for patient safety and management. Contamination of serum or lithium heparin blood with ethylenediaminetetraacetic acid (EDTA) salts may affect the accuracy of some key analytes and compromise patient safety.Combined 15 tubes containing lithium heparin and one tube of K2 EDTA and divided into 5 equal aliquots.Then add the whole blood from the K2EDTA tube in scalar amounts to the autologous heparinized aliquots to obtain varying degrees of contaminated K2 EDTA blood volume ( 0%; 5%; 13%; 29%; 43%). The following clinical chemistry parameters were then measured in centrifuged aliquots: alanine aminotransferase (ALT), bilirubin (total), calcium, chloride, creatinine, iron, lactate dehydrogenase (LD), lipase , magnesium, phosphate, potassium, sodium. Results show significant decreased in calcium, chloride, iron, LD, magnesium and increased potassium starting with 5% K2 EDTA contamination.Phosphate increased after 13% K2EDTA contamination while sodium increased after 29%. Values for ALT, bilirubin, creatinine and lipase remained unchanged until K2 EDTA contamination reached 43%.Compared changes to ideal quality specification,deviation were significant for calcium, chloride, LD, magnesium, potassium from 5% K2 EDTA contamination,and sodium, phosphate,iron from 29% K2 EDTA contamination. Concentrations of calcium, magnesium, potassium, chloride, and LD appear to be big biased even with 5% K2 EDTA contamination . Iron, phosphate, and sodium values remained reliable up to 29% K2EDTA contamination, while ALT, bilirubin, creatinine, and lipase appeared to be less susceptible to K2 EDTA contamination overall. Therefore, when saving blood collection tube additives, it is necessary to form a complete quality system according to the requirements of the corresponding product standards of the additives. In order to ensure that the additives can be used safely and effectively,the product’s MSDS should be prepared according to the requirements of GB/T16483.Material tolerance should be confirmed based on GB18280,then cobalt 60 irradiation sterilization followed.The blood collection tube additives produced by Desheng are produced and stored in strict accordance with the requirements of Pharmacopoeia and MSDS. 

How to Detect Moisture of New Trinder’s reagent The new Trinder's reagent is a commonly used as chromogen substrate in the enzymatic photometric biochemical detection. It belongs to the peroxidase system. After the substrate is oxidized, it has a specific detection wavelength, which is highly sensitive and easy to operate. There are more than ten kinds of reagents, including TOOS, ADPS, MAOS, etc.All of them are stable in the form of hydrates, and the moisture content of them can be measured by Karl Fischer Technology.   Characteristic of new Trinder’s reagent This type of reagent is a derivative of highly water-soluble aniline sulfonic acid sodium salt.Modified with functional groups on the basis of traditional phenol or aniline,the water solubility and stability of new Trinder’s reagents are significantly improved. It should be noted, however, that the new Trinder’s reagents still can be oxidized slowly in air. Such reagents is relatively existing stably in the form of crystal water .In order to increase the stability, it is generally stored in a low temperature and dark place. Such as TOOS, TOPS, ADPS and other chromogen substrate reagents, some of the molecules will carry crystal water. After they are formulated into a solution for a long time, the color of solution will change with slowly oxidized by the oxygen in the air, so it is generally configured before used.   Karl Fischer Technology for Moisture detection for New Trinder’s reagent If the new Trinder’s reagent need to be stored stably, its moisture content should be neither too low nor too high, usually 3%-8% is suitable.So it needs to measure the moisture. Karl Fischer is the authoritative and commonly used method for moisture determination, which has been improved over the years to increase the accuracy and expand the measurement range. It is the standard method for moisture determination of various reagents including Trinder’s reagent.   Principle of Moisture Determination Karl Fischer method belongs to iodometric method, and its basic principle is that when using iodine to oxidize sulfur dioxide, a quantitative amount of water is required to participate in the reaction:I2+S02+2H2O=2HI+H2SO4 The above reaction is reversible. In order to move the reaction in a positive direction and proceed quantitatively, an alkaline substance must be added. Experiments show that pyridine is the most suitable reagent, and pyridine also has the effect of combining with iodine and sulfur dioxide to reduce their vapor pressure. Therefore, methanol or another solvent containing an active hydroxyl group must be added to convert pyridine sulfate anhydride into stable methyl hydrogen sulfate pyridine. The above briefly introduces the moisture detection method of the new Trinder's reagent.In addition to TOOS, TOPS and MAOS, the other biological buffers such as Tris and Bicine produced by Desheng Biochemical can also use Karl Fischer for moisture determination.   For more information,welcome to inquire http://www.whdsbio.cn/product/13.html

What Luminol can do in Criminal Investigation? In criminal investigation dramas, it is easy to see this scene. When polices search at the crime scene and spray something on the ground or in the corner,a certain place lit up after a while, , and the police shouted solemnly or excitedly, "I found it!" .As an editor of Desheng, I like watching criminal investigation films very much. Every time they inspect the crime scene, simulate the case, poke the layers of clues, and finally catch the prisoner, I feel really amazing! So, as a true fan of criminal investigation films, let me lead you to reveal how Luminol detects bloodstains in the reconnaissance scene!     The above image is a scene where the shape of bloodstains is detected, but these bloodstains are invisible, they are displayed by a specific method. In fact, the case is normal in many detective scenes.The bloodstains at the crime scene are often handled or cleaned. It is invisible for us, not to mention to watch the shape of the bloodstains.But if we use special methods to show these bloodstains,you can make a more effective judgment on the case according to the shape and amount of the bloodstain. It turns out that this was the famous Luminol reaction in criminal investigation, which is used to detect bloodstains at the crime scene. Principle of Luminol luminescence First,sodium hypochlorite(NaClO) oxidizes luminol to make it glow; Second, hydrogen peroxide(H₂O₂) reacts with sodium hypochlorite ( NaClO) to generate oxygen to oxidize luminol to make it glow: First is sodium hypochlorite reacts with hydrogen peroxide,NaClO + H₂O₂ == NaCl + O₂ + H₂O. Then , when luminol reacts with hydroxide and forms a double negative ion (Dianion), which can be oxidized by the oxygen decomposed by hydrogen peroxide, and generate an organic peroxide that is very unstable and immediately decomposes to nitrogen (Luminol is oxidized by an organic oxidant such as dimethyl sulfoxide to form nitrogen-containing organics instead of nitrogen) to generate excited 3-aminophthalic acid. In the transition from the excitation to normal state, the released energy exists in the form of photons with a wavelength in visible blue light. Shortcoming of Luminol First,luminol fluoresces with copper, copper-containing alloys, or certain bleaches, and misleading the existence of blood. Second,luminol fluoresces with animal blood and blood in urine, so if the room to be tested contains urine or animal blood, the test results will be biased.Third,Luminol reacts with excrement and emits the same blue light as it reacts with blood. Ways to Avoid Interference when using Luminol 1. After drying a few days of crime scene, the disturbing effect of bleach will disappear, and luminol will still glow with blood even after many years . 2. Use a compound that inhibits the interference effect of hypochlorous acid. Suitable inhibitors have been found for the chemical structure of hypochlorous acid contains with chlorine atoms.   Knowing that bloodstain detection in criminal investigation is using luminol,you’ll find that chemistry is really amazing. luminol reagent is used not only on biochemical and metal ion detection, but also as a marker in carboxylic acid and ammonia compound.With very high sensitivity,it is used in detecting bloodstain in criminal investigation. Luminol produced by Desheng is a light yellow powder. It needs to be dissolved with lye when used and stored in the dark place! For more information,welcome to inquire http://www.whdsbio.cn/product/13.html

The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic and biochemical tests. The principle is that the tested substances react with enzyme,produce hydrogen peroxide (H2O2) which synthesized red Quinoneimine compound with the help of 4-aminotipyrine (4-AAP) and peroxidase (POD).Initially, phenol was used as a chromogen agent, and later, there were many kinds of compounds to replace phenol, which greatly improved the sensitivity of the reaction. There are many principles of in vitro diagnostic reagents or kits. For example: Enzyme-Linked ImmunoSorbent Assay (ELISA), enzymatic method, colloidal gold method, western blotting method, etc. Each methods can be applied to the detection of multiple substances. For example, based on the principle of Trinder reaction (also called enzymatic method), it can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglyceride, etc. There are several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder's reagents are stable enough to be used in both solution and experimental pipeline detection systems. With the help of hydrogen peroxide and peroxidase in oxidative coupling reaction, the new Trinder's reagent reacts with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfonehydrazone (MBTH),and forms very stable purple or blue dyes. The molar absorbance of the dye coupled with MBTH was 1.5-2 times higher than that coupled with 4-AA.However, the solution of 4-AA was more stable than the MBTH.The substrate is oxidized by oxidase and produce hydrogen peroxide. The hydrogen peroxide concentration corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled by the new Trinder's reagent and 4-AA. There are 10 new Trinder's reagents available in Desheng. TOOS is the most commonly used among them. However, for a specific substrate, testing different types of Trinder's reagents is necessary to develop the optimal detection system.

Compared with traditional techniques such as radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA) is a new technology based on chemiluminescence (CL) reaction, which has attracted much attention in the past decade.Due to the associated wide dynamic range and high sensitivity, this technique quickly become the predominant method for clinical immunological analysis. It is widely used in tumor, infectious disease, metabolic disease and pregnancy detection. There are three kinds of chemiluminescence immunoassay (CLIA). First one is chemiluminescence immunoassay (CLIA) Commonly used labeling substances are isoluminol, acridine esters, these labels can generate special luminescent groups and directly participate in the reaction during the analysis process. Directly labeled chemiluminescence immunoassay with acridinium ester: After taking immune reaction with the corresponding antigen (antibody) in the sample to be tested, a solid-phase coated antibody-antigen acridinium ester-labeled antibody complex substance. The oxidant (H2O2) and NaOH make the solvent to be alkaline, and the acridine ester decomposes and glow without catalyst.Substances like luminol for Chemiluminescence immunoassay: The luminescence of these substances is oxidation reaction. In alkaline solution, luminol can be oxidized by many oxidants, among which hydrogen peroxide is the most commonly used. Due to the slow reaction of luminescence, some enzymes or inorganic catalysts need to be added. Enzymes are mainly horseradish peroxidase (HRP), inorganic types include ozone, halogen,Fe₃+,Cu₂+,Co₂ and their complexes. Second is indirect luminescence immunoassay Commonly used markers are horseradish peroxidase (HRP, the common substrate is luminol or its derivatives, such as isoluminol) and alkaline phosphatase (ALP, the common substrate is 1,2-dioxane Ethane derivatives, AMPPD), such markers act as catalysts or energy transfer receptors and participate for luminescence reactions. Third one is electrochemiluminescence immunoassay technology A commonly used marker is ruthenium terpyridine with tripropylamine as a commonly used substrate. The three luminescence technologies have their own merits and are widely used in the diagnosis of different diseases. Chemiluminescence immunoassay technology is of great significance in the field of clinical diagnosis, and chemiluminescence reagents play a huge role. The isoluminol and acridine ester series products produced by Desheng Biochemical have stable quality and are supported by excellent scientific researchers and after-sales teams. 

Viral Transportation Medium for Covid-19 detection Nucleic acid testing is the standard for diagnosing Covid-19, and it is also an effective measure to accurately prevent and control the pneumonia. The mixed detection work in various places to further improve testing capacity and efficiency. At present, the laboratory adopts "10-in-1 mixed detection" technology for the Covid-19. But there are still a lot of people who don't understand the meaning of single and mixed detection. I will introduce them in detail next.   What is the meaning of single and mixed detection? Single detection: As the name suggests, one person's collection swab is placed in a collection tube at one time. Mixed detection: The collection swabs of 5 or 10 individuals are put into a collection tube at same time.When the test result is negative, all the mixed samples are considered negative, which means that the 10 people in the mixed test are all safe. If it is positive, the relevant departments will immediately isolate the 10 subjects in the mixed collection tube separately, and re-collect a single-tube swab for review, and then determine the positive one.   Which VTM (virus transportation Medium) should be selected for mixed collection? The virus transportation Medium is mainly used for the preservation and transfer of upper respiratory tract virus nucleic acid samples such as nasal swabs and throat swabs. In 2020, with the orderly work of fighting against the COVID-19 nationwide,the epidemic has been effectively controlled. In order to further improve the capacity and efficiency of testing,and effectively meet the needs of normalized epidemic prevention and control, the website of the National Health Commission issued the "Notice on Printing and Distributing the Technical Specifications for 10-in-1 Mixed Detection of COVID-19" on Aug 19. In order to prevent secondary infection, it is stipulated that 6ml of inactivated preservation solution should be used for mixed sampling, while single sampling only requires 3ml.   How accurate is the mix detection? The size of the single collection tube and the mixed collection tube are not the same, and the volume of virus preservation solution inside is also different. Based on the results of a large number of basic experimental researches in the early stage and confirmation of practical operations, the increase in the volume of the mixed preservation solution has no effect on the detection results of weakly positive specimens, so the accuracy is not a problem.   Under what circumstances are single and mixed detection used? Single detection: First, it is applied to key areas (confirmed cases and asymptomatic infections in communities with recent outbreaks, old communities, densely populated communities, floating population gathering areas, and people who have recently left the province), and others not suitable for mixed testing.Second,patients in medical institutions are used with single testing. Mixed detection: It is used for large population samples testing and the overall positive rate of the group is less than 0.1%.   As a professional manufacturer for virus transportation Medium, Desheng can provide two kinds of preservation solutions, inactivated and non-inactivated. Of course, whether it is a single or mixed collection tube, we can recommend the appropriate quantity according to the amount of detection people. Competitive price offered, welcome to inquire.http://www.whdsbio.cn/product/13.html

How To Get Refined Heparin?     For many people, heparin is a medicine. In fact, in addition to medicinal purposes, heparin has many other usages, such as made into additives for in vitro diagnosis,biochemical testing, blood collection and storage, or used as raw material of cosmetics. These heparins are purified and processed to refined heparin before using.   Sourcing of Heparin     The name of heparin is called because it was originally found in the liver. It is a natural anticoagulant substance exist in many organs of mammals, with the highest content in the lung and intestinal mucosa. Heparin is a kind of aminodextran sulfate extracted and refined from pig intestinal mucosa or bovine lung. It is a mixture with a molecular weight range of 3000-30000KD and an average of about 15000KD. Unfractionated heparin fission into aminodextran sulfate fragment,witch are low molecular weight heparin calcium enoxaparin and dalteparin with average molecular weight range of 3000-8000KD.   Prepare Crude Heparin     After fresh pig intestines (or after the natural thawing of frozen pig intestines) are carefully washed with water, enzymolysis is carried out: first,the raw materials are carefully adjusted to the pH value of 8-9 with a small amount of dilute lye under full stirring. Second,enzymatic hydrolysis at 37-40 degrees for 3-4 hours. Third,add about 5% of the total weight of the liquid (containing NaCL 95% min, calcium magnesium calcium salt 0.5% max) to mix evenly, then warming up to 90 degrees and keep for 30 minutes, finely filter. After adjusting the filtrate’s PH around 9.0-9.5, ion exchange adsorption treatment is carried out. Forth,elution, suction filtration, combined supernatant and filtrate, then adjusting PH range to 6.0-6.5, added 1.5 times the amount of 95% ethanol to precipitate overnight. the next day, carefully dried and sucked out the supernatant, collected the lower sediment, suction filtered to dryness (The original liquor can be used in the eluent before adjusting the pH value). After vacuum drying, rough heparin is obtained.   Configure Refined Heparin     Completely dissolving the crude heparin in 2% sodium chloride solution to make a solvent with a solubility of about 8%. The temperature can be appropriately raised to help dissolve during this process. Adjusting the PH of above solvent to 8.0-8.2 with 5mol/L sodium hydroxide solution, and raising temperature to 78-80 degrees, then adding 0.15-0.2mol/L potassium permanganate solution for oxidation. Finely filtrated by sterilizing filter, precipitated with 3-4 times of 95% ethanol.Last but not least, dehydrate with acetone, grind to fine, and dry in far-infrared vacuum (50-60 degrees) to obtain fine heparin sodium.         It can be seen from the above that the fined heparin sodium can be obtained from crude heparin after further refining.Hubei New Desheng Material Technology Co.,ltd is specialized in heparin used for blood tube collection, enoxaparin and cosmetic.For more information, pls contact visit our website.http://www.whdsbio.cn/product/13.html