China Wuhan Desheng Biochemical Technology Co., Ltd Company News

What is carbomer? In fact, many of us don't understand. If we are to analyze Carbomer from a professional perspective, it is estimated that many people may still have no way to understand it. After all, people who don't know him can't imagine it at all. But this is not the point. The point is that everyone only needs to understand where the efficacy and role of carbomer are reflected in our lives, and then put it to good use. The efficacy and role of carbomer 1. Protect the skin Carbomer has an obvious protective effect on human skin. It has a certain affinity for human skin. People usually add it to cosmetics. It can integrate various ingredients in cosmetics and keep them stable. In the right state, and the cosmetics with carbomer will have a good base, and people will feel particularly comfortable after applying it on the skin. It can protect the skin, and can reduce the irritation and damage of irritating substances to human skin and skin mucous membranes, and can prevent a variety of allergic symptoms from occurring.   2. Anti-ultraviolet It can well resist the irritation caused by ultraviolet rays to the skin. Why is this? Because carbomer has a certain activity, after it is applied to the surface of human skin, it can enhance the resistance of human skin to ultraviolet rays and reduce the damage of ultraviolet rays to human skin. When used in summer, carbomer's sunscreen products will be added. Play a particularly ideal sunscreen effect, can prevent the skin from darkening and also prevent the skin from being burned by ultraviolet rays.   3. Reduce the viscosity Carbomer has a certain degree of porosity, and it is a slightly acidic substance with particularly strong hygroscopicity. Usually when making gels or cosmetics, you can add an appropriate amount of carbomer, which can reduce the viscosity of these substances. , You know, it is a very hygroscopic substance, so if it is added to cosmetics, it will make the cosmetics less viscous and maintain their stable properties.   4. Anti-inflammatory and sterilization Carbomer is also a natural medicinal ingredient. It can reduce inflammation and sterilization. The carbomer eye drops sold in the medical market are medicines made with it as the main raw material. It has a good effect on human eye inflammation. Effect, can make the patient's pain and eye redness and swelling disappear quickly.   From the above, these are just some of the uses of carbomer in cosmetics, and it is not just used in cosmetics! If you have a need or are planning to use Carbomer to develop other products, you can contact us. Our Desheng company is a manufacturer specializing in the development and production of Carbomer.

In terms of application in various fields, the acridinium ester luminescence immunoassay technology is of great help. According to the experimental principle of the double antibody sandwich method, the acridinium ester chemiluminescence immunoassay technique combined with the biotin-avidin magnetic particle separation technology is established. A detection method for quantitative determination of carcinoembryonic antigen content in serum.   Due to the application of acridine ester luminescence immunotechnology, the medical determination of carcinoembryonic antigen content in serum is more rapid and simple, so that carcinoembryonic antigen patients can be treated in time, which provides great convenience and makes a major step forward in medicine. step. Acridine Ester Product Picture It was first discovered that the phenomenon of chemiluminescence was found in organisms, such as fireflies, now called bioluminescence, which is named after the visible light emitted by organisms. Smart people will never give up after discovering this phenomenon. Later, after many experiments, it was found that the reaction of N,N-dimethyl diacridine nitrate and hydrogen peroxide can produce chemiluminescence. In many cases, the measurement of glucose is used as an example. It has been applied in the determination of reducing substances.   Since then, the discovery of the chemiluminescence phenomenon of acridinium ester derivatives, the synthesis of chemiluminescence labeling reagents and the chemiluminescence immunoassay have become a popular research content in the 1980s, which promoted the application of this type of analysis method in the analysis of human body composition.   Acridine-based chemiluminescence systems are widely used in the fields of inorganic and organic compounds, biological and pharmaceutical analysis due to the advantages of no catalyst, mild reaction conditions, and good reproducibility.   Desheng is an established chemical reagent company specializing in the development and production of blood test reagents. Desheng acridinium ester has 6 products with different groups. In addition to acridinium ester, chemiluminescence reagents also include luminol and isoluminol. The luminous efficiency of the acridine ester series is relatively high and belongs to direct luminescence.

N,N-bis(hydroxyethyl)glycine, alias Bicine, CAS number is 150-25-4, Bicine is a biological buffer; biological buffer is recommended for low-temperature biochemical work. Used to prepare a stable substrate solution for serum guaninase determination. It is also a very versatile Buffer, used for enzyme reaction buffer, electrophoresis buffer, working concentration from 3-100mM. Bicine is often used as a buffer solution for capillary electrophoresis. N, N-Dihydroxyethylglycine Bicine is a zwitterionic buffer, belonging to Good’s buffer, with a pH buffer range of 7.6-9.0. The molecular structure of Bicine is similar to that of glycine. The molecule has a substituted amino group, a carboxyl group and two hydroxyl groups as coordinating groups, so it has the properties of an amino acid and aminoethanol at the same time, and can form a metal complex with a single anion (Bic-) , Is often used as a buffer solution for capillary electrophoresis.     Bicine is an effective inhibitor for the oxidative degradation of amines. According to the patent CN104245089A description, Bicine is an effective inhibitor for the oxidative degradation of amines, and can effectively inhibit the oxidative degradation of the amines used or the amine-containing absorbents used.   Bicine is used as a chelating agent for soil remediation. Bicine is an amino acid analogue that is easily soluble in water. It is an environmentally friendly substance. It contains two -OH and one -COOH in its molecule. It has good chelating properties and can chelate heavy metal ions such as Cu, Cd, and Pb, but Can not chelate calcium and magnesium ions. Therefore, Bicine is often used as a chelating agent to repair soil contaminated by heavy metals. Bicine has a good elution ability for heavy metals under neutral and acidic conditions, and can efficiently remove Cu, Cd, and Pb ions in contaminated soil. Bicine is an eluent of active components, which can not only wash and repair heavy metals. It pollutes the soil, and can avoid the loss of plant nutrient elements calcium and magnesium in the soil, is safe and environmentally friendly, and will not cause secondary pollution to the environment. Bicine is also a widely used in vitro diagnostic reagent raw material, suitable for biochemistry and medicine. In the field of in vitro diagnostics, it is an important raw material for antibody detection reagents and nucleic acid detection reagents.

Before understanding Virus Transport Media, let’s first understand which viruses are made up of. Viruses are composed of a nucleic acid molecule and protein or only protein. Individuals are small and simple in structure. Common epidemic infectious diseases are usually RNA viruses, such as influenza virus, HIV, influenza A virus. Because there is no cell structure, the virus cannot replicate itself, but invades its genes into the host cell, using the latter's replication system to replicate new viruses. After the virus sample is collected, in order to maintain the viability of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be stored and transported in Virus Transport Media. Virus Transport Media has two types: inactivated and non-extinguishing. The inactivated Virus Transport Media uses high-concentration guanidine salt to quickly inactivate and preserve respiratory pathogens, making the sample lose infectivity. The inactivated samples can be used with a variety of viral DNA/RNA extraction kits. The M32/M96 nucleic acid extractor can quickly extract nucleic acids. At the same time, it can be used with the respiratory pathogen PCR detection kit to achieve rapid detection, and the specific sensitivity is not affected.     The non-inactivated Virus Transport Media contains Hanks liquid base, gentamicin, fungal antibiotics, BSA (V), cryoprotectant, biological buffer, and amino acids. A combination of multiple antibiotics has anti-bacterial and anti-fungal effects; Bovine Serum Albumin (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus; Hanks The neutral environment constructed by the buffer helps increase the survival time of the virus and the stability of infection. The non-inactivated Virus Transport Media is usually used for the collection and transportation of clinical influenza, avian influenza (such as H7N9), hand-foot-mouth disease, measles and other virus specimens, as well as mycoplasma, ureaplasma, chlamydia and other specimens. The sudden new crown epidemic has caused serious impacts on many companies. As high schools and junior high school students in major regions resume school, the demand for new crown pneumonia nucleic acid testing is increasing. Virus Transport Media, as one of the indispensable products for nucleic acid testing, has been greatly affected. Major companies are concerned. Since the resumption of work and production, Desheng Technology has been committed to the research and development and production of Virus Transport Media, blood collection tube additives and other products. It has made considerable progress and its products have been well received at home and abroad!

Depending on the degree of crosslinking and molecular weight, Carbopol has a variety of products of different types, including traditional series such as Carbopol 910, 934, 934 P, 940, 941, and 954, as well as new polymers such as Carbopol Ultrez 20/21 . Different types of carbomers have different uses in various industries due to their suspension stability characteristics, fluidity change length and other properties. Let's take a look at the properties and applications of the most commonly used carbomers in cosmetics. · Traditional carbomer Carbopol 934 (benzene solvent homopolymer) Appearance: white loose powder, slightly odor Performance: short rheology; medium and high viscosity; medium transparency, slightly yellow; low ion resistance; high shear resistance; suspension stability, high heat resistance Safety: There may be residual solvents (benzene or xylene) Application: Suitable for high-viscosity gels, lotions and ointments; suspension and emulsification; topical application; skin care; hair care; taste masking agent; cream; hand, body and face lotion. It is widely used in medicine (ointment) formulations and cosmetic creams. · Carbopol 940 (benzene solvent homopolymer) Appearance: white loose powder, slightly odor Performance: (jelly type) very short rheology; high viscosity; high transparency; low ion resistance; low shear resistance; high yield value (suspension ability) Solubility: soluble in water, glycerin and ethanol, etc. Safety: No mold will grow when dry, non-toxic and non-irritating Application: It can provide the stability of the oil-in-water emulsification system and is suitable for use in lotions and creams. Suggested dosage: 0.2% ~ 1.5% · Carbopol 941/971 (benzene solvent homopolymer) Appearance: white loose powder, slightly odor Performance: (snot type) long rheology; low viscosity; high transparency; medium ion resistance; low shear resistance Solubility: soluble in water, glycerin and ethanol, etc. Application: It can produce low-viscosity permanent emulsions and suspensions, even ionic systems, and the resulting colloids have good transparency (more effective at low concentrations). Carbo 941 is used as an oil-in-water emulsification system in the cosmetics industry. Stabilizer, 971 is used as a slow-release agent in the pharmaceutical industry, suitable for transparent gels, hydroalcoholic gels and emulsion products. · ※Improved carbomer (no benzene) Carbopol 980 (co-solvent homopolymer) Chemical name: Cross-linked polyacrylic resin Appearance: white loose powder, slightly odor Performance: very short rheology; high viscosity; high transparency; low ion resistance; low shear resistance; high yield value (suspension ability) Solubility: soluble in water, glycerin and ethanol, etc. Application: Suitable for thickening, suspending and emulsifying in cosmetics or pharmaceutical formulations. Such as: styling gel, hydroalcoholic gel, moisturizing gel, shower gel, toothpaste, shampoo, shaving gel, moisturizing cream and sunscreen lotion, etc. Dosage: 0.2% ~ 1.5% · Carbopol 1342 (hydrophobic modified copolymer) Chemical name: Cross-linked polyacrylic resin Appearance: white loose powder Performance: long flow characteristics; medium viscosity; high transparency; high ion resistance; high shear resistance; high yield value (suspension capacity) Solubility: soluble in water, glycerin and ethanol, etc. Application: Excellent rheology modifier in the presence of electrolyte, polymerization and emulsification, suitable for aqueous solutions or dispersions containing water-soluble salts. · Carbopol ETD 2020 Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked Copolymer Appearance: white loose powder Performance: long rheology; medium viscosity; high transparency; high ion resistance; shear resistance; efficient thickening, strong suspension ability Application: Suitable for transparent gels, hydroalcoholic gels, surfactant systems (special shampoos, cleaning products, etc.), and systems with high electrolyte content (such as aloe gel), etc. Suggested dosage: 0.2% ~ 1.5% · Carbopol AQUA SF-1 New liquid acrylic rheology modifier INCI name: acrylic (ester) copolymer Appearance: milky white to white liquid Performance: long rheology; high viscosity; medium transparency (the body is opaque, after adding the product); low ion resistance; high shear resistance Solubility: soluble in water, glycerin and ethanol, etc. Application: It can be formulated into a clear formula, has excellent compatibility with a variety of ingredients, acidified and thickened, and can be used in surfactant systems. · Carbopol Ultrez 20 (new easily dispersible copolymer) Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked Copolymer Appearance: white loose powder Performance: long rheology; high transparency; medium viscosity; medium ion resistance; high shear resistance; easy to disperse, excellent and stable suspension ability Application: It can be used in shampoos, shower gels, creams, lotions, and hydrogels with high electrolyte content. In addition, U20 can also give the product a bright appearance, with better fluidity and pourability, providing the product with a smooth, light, and soft touch. · Carbopol Ultrez 21 (new easily dispersible copolymer) Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked Copolymer Appearance: white loose powder Performance: short rheology; high viscosity; high transparency; low ion resistance; shear resistance; easy to disperse, self-wetting, high-efficiency thickening, strong suspension ability, can give the formula a constant viscosity. Application: It can be used for gel, washing and cleaning products, high electrolyte products, etc. · Pemulen TR-1 (hydrophobic modified copolymer) Chemical name: Acrylate/C10-30 Alkyl Acrylate Cross-linked Copolymer Appearance: white powder Performance: short rheology Application: Multifunctional thickening emulsifier, used as a stabilizer in oil-in-water systems, and can be used in creams and lotions. Suggested dosage: 0.2% ~ 0.4% to achieve 20.0% oil content. · Pemulen TR-2 (hydrophobic modified copolymer) Chemicals: acrylate/C10-30 alkyl acrylate cross-linked copolymer Appearance: white powder Performance: long rheology Application: Multifunctional thickening emulsifier, used as a stabilizer in oil-in-water systems, and can be used in emulsions. Suggested dosage: 0.15% ~ 0.30% to achieve 60.0% oil content.

Desheng company is established for 15 years, focusing on R & D, production and sales in one of the old company, R & D and production of a complete range of products, price concessions, now on our company's R & D and production experience α- Glucosidase is a simple explanation.   Glucosidase is one of the major enzymes in glycoside hydrolases (EC 3.2.1). It is named because it can hydrolyze glucoside bond and release a molecule of glucose.   Glucosidase is one of the important members of glucose metabolism pathway. β- Glucosidase is involved in the metabolism of cellulose and many physiological and biochemical pathways, α- Glucosidase is directly involved in the metabolism of starch and glycogen. It has double functions of hydrolysis and transglycoside in the catalytic reaction of sugar. Hydrolysis can be carried out from α- Non reducing telostomy of glucosides, oligosaccharides and glucans α- 1,4 glycosidic bond, releasing glucose; In addition, the free glucose residues can be separated by transglycosylation α- Non fermentative Isomaltooligosaccharides (IMO) were obtained by transferring 1,6 glycosidic bond to another glucose or maltose substrate. The abnormal function of these enzymes will lead to metabolic diseases. At the same time, these enzymes are also the targets of many drugs and inhibitors to regulate the glycochemical metabolism in the human body.     α- Glucosidase α- Glucosidase can be used to screen active natural drugs; one α- Immobilization of glucosidase: using trimethylol phosphorus as crosslinking agent and chitosan as carrier α- Glucosidase;   2. Make inhibitor screening model: immobilize the above-mentioned inhibitors α- The glucosidase was loaded into a column with a diameter of 0.8 cm and a length of 8 cm, in which the pH 6.8 potassium phosphate buffer was added and stored at 4 ℃;   3. Validation of the model: a representative α- Acarbose, a glucosidase inhibitor, was used to verify the effectiveness of the screening model. The lower end of the column was tightly inserted into a small tube with a piston, and a syringe was inserted into the upper end. The buffer solution in the column was pressed to about 0.4cm below the top of the column, and then 50 μ L 4-nitrobenzene- α- D-glucopyranoside (0.116mol / L) and a certain amount of acarbose solution (250mg / L), gently shake to make the solution at the top of the column mix well, then continue to press the solution until the liquid level is level with the top of the column, close the bottom piston, then put the column into the water solution tank, incubate at 37 ℃ for 10 minutes, after the reaction is completed, use 5ml Wash the column with ph6.8 potassium phosphate buffer, collect the washing solution, dilute the eluent to 10ml volume, and determine the absorbance value at 400nm with ultraviolet spectrophotometer; And the above 50 μ L 4-nitrobenzene- α- D-glucopyranoside, acarbose solution and 5ml buffer solution were diluted to 10ml as blank, and the inhibition rate of acarbose was calculated according to the absorbance data of the obtained reaction solution;   4. Immobilization α- Screening model of glucosidase for water soluble fraction of traditional Chinese medicine Polygonum cuspidatum α- Objective to screen the water-soluble fraction of Polygonum cuspidatum. We Desheng R & D and production of enzyme preparations used in the kit, customers with relevant needs are welcome to consult.

Carbomer is also known as Carbopol It is a kind of high molecular polymer which is crosslinked by acrylic acid and acryloyl sucrose or acryloyl pentaerythritol.   Carbomer is actually a very common emulsifier. Specifically, Carbomer can easily emulsify any oily liquid at a temperature of about 25 degrees, and the whole emulsification process takes a very short time. Second, it also has a good use in skin care products, because it is a very good stabilizer.     Carbomer 940 compared to 980 permeability better viscosity, but if you need to prepare 75% free gel, you need to use carbomer 980, because carbomer 940 can only be applied to the concentration below 65%. Desheng company is a professional manufacturer of carbomer 940 and 980, welcome to ask for samples!

4-Hydroxyethylpiperazine ethanesulfonic acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research. As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products: 1. Advantages of PH range: The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture. 2. Purity advantage: Desheng HEPES has a purity of ≥99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services. 3. Advantages of reserves: Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment. 4. Export advantages: Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention. 5. HEPES packaging advantages: We can pack in quantities according to customer needs. Generally, there are 500g small bottles and 25kg cardboard drums. The big packaging is covered with plastic bags, the white powder is packed in the belt, and the tie is tight. 6. Recommended storage: HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not degrade due to autoclaving. However, if the HEPES aqueous solution is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H2O2) will be produced. Therefore, it should be noted that the HEPES aqueous solution must be kept away from light to avoid affecting the experimental results. HEPES powder is generally placed in a dry room when it is placed, and cannot be exposed to direct sunlight for a long time. It is best to store it at room temperature 2-8°C.   Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.

The full name of EDTA-2K is dipotassium ethylenediaminetetraacetate. It is a white crystalline powder and belongs to a common complexing agent. Because it can complex with the calcium in the blood to inactivate the calcium and further cause the blood to not coagulate, it is usually used to prepare anticoagulants and mainly used in the blood collection tube industry. EDTA has two CAS numbers, mainly because one of them is actually EDTA dipotassium crystalline hydrate. People often mistake the CAS numbers of these two EDTAs, which makes people confused. The real situation is that the CAS number of EDTA-2K is 2001-94-7 and the CAS number of EDTA-2K dihydrate is 25102-12-9. The difference between them is that there are two crystal waters in one molecule. And this article is an introduction to the wide range of uses of EDTA-2K.   The International Blood Standards Committee recommends the use of EDTA-2K anticoagulated blood for complete blood counts. The use of EDTA-2K as an anticoagulant in vacuum blood collection tubes can preserve leukocyte hypertrophy in a short time, prevent platelet aggregation, and preserve platelets. In addition to being an anticoagulant, EDTA-2K has many uses in industrial production, environmental optimization and other fields.   EDTA-2K can be used as a new type of gel material magnesium phosphate cement retarder, compared with borax used in traditional magnesium phosphate cement. It has a better retarding effect, can prolong the setting time of the magnesium phosphate repair system, and ensure the strength of the magnesium phosphate cement, making it more widely used in the rapid repair of highways, bridge decks, and airstrips.   In oilfield production, EDTA-2K is also used as a stabilizer to form a desulfurization synergist, which can not only maintain the stability of the desulfurization synergist, but also chelate the calcium and magnesium ions in the operating system to be treated to stabilize the overall operating system. Water quality. The desulfurization synergist can effectively chemically react with the sulfide in the equipment to achieve the purpose of removing sulfide, have a good desulfurization effect, and ensure the safety of oilfield construction.   EDTA-2K is also commonly used to prepare deodorants, such as toilet deodorants, decomposing pesticides and odor molecule removers, etc. In addition, according to the description of related patents, the potassium salt of ethylenediaminetetraacetate is calcined at high temperature, pickled, and washed. Activated carbon can be prepared in processes such as washing and drying.

There are many kinds of blood anticoagulants in blood collection tube reagents, and one anticoagulant has a wide range of uses, that is, potassium EDTA. There are many types of EDTA potassium salt, among which dipotassium is used more, but what about its principle and application? There should be some people who are not very familiar with it. Here we can take a look at what application areas and principles it has. EDTA dipotassium salt is also known as dipotassium ethylenediaminetetraacetate dihydrate (EDTA-K2). It is a white crystalline powder, easily soluble in water, easy to absorb moisture, and has a molecular weight of 404.6. DETA dipotassium salt can protect the cell components of the blood, does not affect white blood cells, has the least impact on red blood cells, and can inhibit the aggregation of platelets, which is suitable for general blood tests. But for the separation test with platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor is it suitable for calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine amino peptide Enzyme determination and PCR test.   The dipotassium EDTA is mostly used in complexing metal ions and separating metals, as well as washing powder, shampoo, liquid soap, agricultural chemical spray, blood anticoagulant and other fields. The dipotassium EDTA is also recommended by the International Committee for Standardization in Hematology as the preferred anticoagulant for blood count and classification.   The principle of EDTA dipotassium salt as an anticoagulant, first of all, we first understand the principle of blood coagulation, which can be divided into three parts, 1. The formation of prothrombin activator, 2. The role of prothrombin activator in calcium ion With participation, prothrombin is converted into active thrombin. 3. Soluble fibrinogen is converted into insoluble fibrin under the action of thrombin. Fibrin is shaped like filaments, criss-crossed, and collects a large number of blood cells to form a jelly-like blood clot. This is how coagulation occurs. The dipotassium EDTA has a great affinity for calcium ions in the blood, and can complex with calcium ions so that prothrombin cannot be converted into thrombin, thereby making the blood anticoagulant.

Recently, some customers reported that the EHSPT (also called TOOS) solution prepared by myself was transparent, but it turned yellow or red after being left for two days. What is the reason? Can it still be used?   Reasons for discoloration of Trinder's reagent solution: Chromogenic substrates such as EHSPT and MAOS are all new Trinder’s reagents, which are all derivatives of highly water-soluble sodium aniline, which will produce red or yellow substances when oxidized. Trinder's reagent powder is not easy to be oxidized. It needs to be sealed and refrigerated after preparation. If the solution is exposed to the air for a long time and the temperature is high, they may be slowly oxidized by the oxygen in the air. Discoloration of the solution occurred. Trinder’s reagent for enzyme-linked immunoassay   Trinder's reagent detection principle: Trinder's reagents such as EHSPT and MAOS can be oxidized by hydrogen peroxide (TOOS, MAOS, etc. need to be coupled with 4-AAP) in the presence of peroxidase to produce red quinone imine substances, which can be detected by measuring absorbance with a photometer The content of the test sample that can generate or oxidize hydrogen.   The new Trinder’s reagents are all reductive color-developing reagents. They are originally colorless. When they are catalyzed by peroxidase by oxygen, they can generate colored oxidation products. However, chromogen substrates such as EHSPT are relatively unstable in the solution. They are contaminated by oxidizing impurities or exposed to air for a long time, which will cause them to slowly oxidize, and the solution will change color. Therefore, when the chromogen substrate is formulated into a solution, it is generally recommended to prepare it for immediate use to prevent it from being contaminated or oxidized.   The advantages of Trinder's reagent compared to TMB: When using TMB as a color reagent, the detection wavelength is 450nm maximum absorption wavelength and 405nm wavelength, and the absorption wavelength of bilirubin in the serum is 380~530nm, which will interfere with the detection results, and the color should be done under acidic conditions. Reaction limits its application. The UV absorption of the color reaction product of the new Trinder’s reagent is >540nm, MAOS and MADB are even as high as 630nm, and the reaction requires a wide pH range, and is less interfered by other substances in the serum. It can be used for detection items that require high precision values.   At present, in addition to chemiluminescence immunoassays, enzyme-linked immunoassays are more commonly used. The new Trinder's reagent is one of the important chromogenic substrates. Desheng is engaged in the R&D, production and sales of acridinium ester chemiluminescence reagents and new Trinder's reagents. All have extensive experience.

In biochemical testing, the detection of blood glucose, blood lipids, liver and kidney function, etc. usually adopts enzymatic photometry. Many detection principles are based on Trinder reaction. The required chromogen substrates and enzymes have requirements for the experiment, and the stability is not ideal. Polymer stabilizers to improve.   The chromogen substrates used in the Trinder reaction, such as TOOS, MAOS, ADPS, etc., belong to the sodium salt of aniline sulfonate. After being formulated into an aqueous solution, it cannot be exposed to the air for a long time and will be slowly oxidized. Therefore, it is usually stored as a powder reagent. Temporarily prepare a solution. On the other hand, enzymes have higher requirements on the temperature and pH of the reaction system, and they are prone to inactivation even when the concentration is too low. So improving their stability is very valuable. Chromogen substrate MAOS powder   Through research, it is found that a polymer with a special structure can significantly improve the stability of chromogen substrates and enzymes. The polymer has the general structural formula of R1 R2 R3, R1 is selected from olefin unsaturated groups or olefin unsaturated monomers; R2 is selected from ester group COO or ether group O; R3 is selected from polyethylene glycol, polyoxypropylene, Polypropylene glycol, polyacryl alcohol and other polymer groups.   The application of this polymer can increase its stability by interacting with enzymes and chromogen substrates, so that a variety of in vitro testing items can maintain the stability of enzymes and chromogen substrates under extreme environments (such as high temperatures). The detection result has less deviation and higher accuracy. It can be combined with other components to form a stable enzyme and chromogen substrate composition. It can be used in enzyme-catalyzed color reaction and in the preparation of in vitro detection products based on the principle of enzyme-catalyzed color reaction. And the preparation of enzymes and chromogen substrate stabilizers.   Applicable enzyme preparations include: peroxidase such as POD or HRP, cholesterol esterase, cholesterol oxidase, alkaline phosphatase, creatine kinase, glycerol kinase, phosphoglycerol oxidase, lipoprotein lipase, urate oxidase, acetone One or two or more of acid oxidase, glucose oxidase, creatinine hydrolase, and creatine hydrolase.   Trinder's chromogen substrates suitable for polymer stabilizers include: TOOS, TOPS, MAOS, MADB, etc. and their combinations 4-AAP, MBTH. The above-mentioned chromogen substrates and enzymes are commonly used reagents in enzymatic photometry. Desheng is a manufacturer and can provide a variety of chromogen substrates and enzyme preparations.