China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Dipotassium ethylenediaminetetraacetate, also known as EDTA K2, is a white crystalline powder on the surface. It is a common complexing agent because it can complex with calcium in the blood to inactivate calcium, thereby preventing blood with coagulation purpose.It is often used in the preparation of anticoagulants in vacuum blood collection tubes.   There are two CAS numbers of dipotassium EDTA, one of which is dipotassium EDTA crystal hydrate, the CAS number is 25102-12-9, and the CAS number of dipotassium EDTA is 2001-94-7, many people will confuse these two CAS numbers. You need to pay special attention to the difference is that one molecule differs by two crystal waters. Desheng will give a general introduction to the scope of use of Dipotassium EDTA.   1. Used in vacuum blood collection tubes In recent years, with the upgrading of blood testing, Dipotassium EDTA has been widely used in blood collection tubes. As an anticoagulant, it can rapidly store leukocyte hyperplasia for a period of time, prevent platelet aggregation, and prevent blood from coagulating , which not only facilitates medical staff to improve blood testing efficiency, but also promotes more accurate test results.   2. Used in industrial production In addition to the common use for blood anticoagulation, dipotassium EDTA can also be used in the field of industrial production and can be used as a buffer. When used in gel engineering, it not only has excellent retardation effect, but also can prolong the time of the repair system and ensure a certain strength, so that it can be quickly repaired on bridge decks, highways, etc.   3. Used in oilfield production In oilfield production, dipotassium EDTA can be used as a stabilizer, which can not only chelate calcium and magnesium ions to stabilize the entire operating system, but also constitute a desulfurization synergist, so that the desulfurization synergist can effectively interact with the sulfide in the system. A chemical reaction is generated to effectively remove sulfides and ensure production safety.   4. Used in daily chemicals Dipotassium EDTA can also be used in deodorants or detergents, liquid soaps, and has a significant effect on deodorizing at home. In addition, according to research, dipotassium EDTA can also be used to prepare activated carbon.   Desheng Biochemical is the source manufacturer specializing in the production of dipotassium ethylenediaminetetraacetate. The series of products produced are stable in performance, and the quality is supervised and controlled by professionals. The products produced in the same batch will be tested first before delivery. If you have a demand for blood collection tube additives, please click on the website for inquiry!

When many people go to the hospital to check their health, they will find blood routine must be tested.Most medical staff will judge whether the patient has a hidden disease or aggravated disease based on the analysis of the patient's blood sample. As a blood anticoagulant, Dipotassium EDTA is often added to VBCTs. It has the advantages of good anticoagulation effect and less influence on the morphology of blood cells. Next, I will introduce you anticoagulation principle of potassium in vacuum blood collection tubes.   In fact, if you want to understand the anticoagulation principle of dipotassium EDTA, you need to know 4 steps of blood coagulates. 1. The production of prothrombin activator. 2. The next step is that prothrombin becomes active under the influence of calcium ions and phospholipid membranes. 3. Under the above two steps, fibrin is formed. Due to the participation of thrombin, soluble fibrinogen is converted into insoluble fibrin. 4. After the formation of fibrin, thrombin will be adsorbed, and local blood clots will form.   These four steps are roughly the process of blood coagulation.Dipotassium EDTA,a white crystalline powder is used as a complexing agent. Since dipotassium EDTA is close to calcium ions, it can complex and block the formation of thrombin from prothrombin, that is, the second step of the above blood coagulation process is suspended, which prevents blood coagulation from the source, and subsequent coagulation process cannot continue.   Due to its anticoagulant advantages, Dipotassium EDTA is regarded as an ideal anticoagulant in routine blood analysis, but some issues need to be paid attention to when using it, such as the ratio of blood to Dipotassium EDTA. Disproportion can cause a slight clotting phenomenon, which can block the blood analyzer or cause inaccurate test results. In addition, in order to ensure sufficient anticoagulation effect, after blood collection, medical staff should mix upside down 5-8 times to ensure that the blood is completely exposed to the anticoagulant to avoid partial coagulation.   Hubei New Desheng has been developing and producing blood collection tube reagents since its establishment. It is a relatively old-fashioned manufacturer. The unit packaging volume of its Dipotassium EDTA anticoagulant is 500g/bottle and 25kg/barrel.Price based on the quantities. So far, the products are sold at home and abroad, the repurchase rate is high. If you have this demand, please click on the website to consult the details!

Dipotassium EDTA is often used in combination with serum separation gel and sodium fluoride   Dipotassium EDTA is mainly used as an in vitro blood anticoagulant and is widely added to vacuum test tubes. It is often used together with serum separation gel and sodium fluoride to make the detection results more accurate and improve efficiency. However, it is not added to the same test tube at the same time, the effect is the same, and the items checked are different for different additives. Below, I will specifically introduce to you the functions of dipotassium EDTA, serum separation gel and sodium fluoride.   1. Dipotassium EDTA + serum separation gel If only dipotassium EDTA is added to the test tube, it is an ordinary blood routine test tube, and its function is to prevent blood coagulation. But it is added to the test tube together with serum separation gel, which is generally used in nucleic acid detection tubes. Nucleic acid detection tubes are used for the collection, transportation and storage of venous blood samples for nucleic acid detection, mainly for DNA amplification detection of HBV DNA, HCV, and HIV, and serum separation gel can well isolate hemoglobin in red blood cells to prevent Nucleic acid detection experiments are interfering.   2. Dipotassium EDTA + Sodium Fluoride Can sodium fluoride effectively inhibit glycolysis and keep blood sugar concentration relatively stable, so it is considered an ideal blood sugar test specimen preservation agent, while dipotassium EDTA can prevent blood coagulation due to its own chelating properties , and the dissolution rate is fast. When used together with sodium fluoride in the preparation of anticoagulation tubes, it can be dried at 100 degrees, and the anticoagulation effect can still be maintained without worrying about being decomposed. The preparation conditions are also easier to control, which is convenient for production to improve efficiency. Dipotassium EDTA is used together with serum separation gel and sodium fluoride. The amount of dipotassium EDTA should be noted. Generally, 4 mg of EDTA needs to be added to anticoagulate 2 ml of blood. For dipotassium acetate, usually 20 μl of an aqueous solution containing 200 g/L of dipotassium ethylenediaminetetraacetate is pre-placed in each blood collection tube. In addition, medical staff are required to turn upside down as soon as possible after blood collection, so that they are fully exposed to the additives, but they should not be shaken too violently, which may easily cause hemolysis. Desheng Biochemical has always been a veteran manufacturer of blood collection tube additives, focusing on a series of products, such as serum separation gel in blood collection tubes, heparin series, EDTA series, to meet the different needs of customers. Today, all kinds of products have been exported to overseas, opening up a "road of raw materials for blood collection tubes" of its own. If you have needs in this regard, please visit our website !

With the development of the medical beauty industry, PRP high-concentration platelet-rich plasma has gradually been paid attention to. It is derived from individual blood, and its main components include platelets, leukocytes and fibrin. The concentration of platelets in plasma is particularly important.   Among them, the PRP separation gel produced by Desheng is helpful for the efficient extraction of platelet-rich plasma. The separation gel has resin system and acrylate system. Why must the resin system be used in the PRP tube? Next, Desheng will show you details.   The traditional method of PRP extraction is to take two centrifugation methods. According to the difference in the proportion of components in the blood, the sedimentation speed during centrifugation is different. The first centrifugation of platelet-containing plasma and red blood cells, and the second centrifugation separates platelets and platelet-poor plasma.   When resin separation gel is added, because the specific gravity of the separation gel is larger than that of platelet-rich plasma, after centrifugation, the uppermost layer in the PRP tube is serum, the second layer is PRP, the third layer is PRP separation gel, and the lower layer is blood cells. As long as the uppermost serum is extracted, PRP can be obtained.   Why choose to use resin release glue? 1.Radiation resistance The resin-type separation gel is a radiation-resistant material, and the PRP tube needs a certain irradiation process. If the separation gel is used, it must be of the radiation-resistant type.   2.Hydrolysis resistance PRP often requires built-in anticoagulants, such as sodium citrate solution or ACD solution, so the gel added to the test tube cannot react with the solution during long-term contact with the solution, and resin-type separating gel can well meet this requirement.   3.The operation is simpler, no need for 2 centrifugation, high-quality platelet-rich, fast extraction, and less blood samples are needed.   Desheng is a manufacturer specializing in the development and production of resin-based PRP separation gel. Its production environment is sterile, and the specific gravity of PRP gel required for different purposes is different, and the specific gravity can also be customized according to customer requirements. With the continuous improvement of process technology, the PRP separation gel produced by the company has excellent performance and is favored by PRP customers at home and abroad. If you have this demand, please click on the website to consult the details, and the company can provide sample testing.

In the early days, when serum separation gels were not fully developed, people generally used specific gravity particles or directly waited for a certain period of time to separate serum and blood clots naturally before testing. This is not only inefficient, but the final test results may not be accurate. In 1990, my country began to introduce blood collection tube additive technology. Although the effect was not ideal, serum separation gel was born and laid the foundation for subsequent development.   It seems that the serum separation gel is a very small plate, but it not only effectively saves time and improves efficiency in the entire blood collection tube, but also ensures the accuracy of blood sample collection.   Serum Separation Gel Parameters: Product Name Serum Separation Gel Place of Origin Made in China Brand Desheng Specification 20kg/drum、25kg/drum Appearance Transparent colloid,no visible impurities Characteristic Physiological Inertia,water-insoluble Gravity 1.045-1.065g/cm3 Volatile ≤1.5%   Functional of serum separation gel Serum separation gel is mainly used in vacuum blood collection tube reagents to form a separation layer between blood cell components and serum, preventing their interaction, ensuring the originality of blood samples within a sufficient time, and making the test results more accurate. With the development of medicine, nowadays, serum separation gel is not limited to the separation of serum and plasma, but can be used in skin grafting, treatment, cosmetic surgery, etc.   Various products in the field of serum separation gel Common serum separation gel raw materials are used in tubes, such as PRP, PCT, SST, PST tubes, and EDTA tubes. What is the role of these serum separation gels in the tube? Let me introduce it to you in detail. 1. PRP tube It mainly extracts the rich platelets by the precise specific gravity of serum separation gel, and then injects the rich platelets into the parts of the human body that are needed to achieve the effect of cosmetic repair, regeneration or treatment. 2. PCT tube The PCT tube is a vacuum mononuclear cell preparation tube, which separates lymphocyte and mononuclear cells in the blood by separating the different specific gravity of the gel, and is used in clinical medical detection. 3. SST tube Separating gel is used to extract high-quality serum, which provides convenience for serum biochemistry, immunology and drug detection. 4. PST tube The separation gel in the PST tube is mainly used to separate blood cells, serum and plasma, to increase the yield and ensure the stability of the plasma composition.   The above four kinds of preparation tubes use more serum separation gels, but many serum separation gels cannot meet the requirements due to their poor hydrolysis resistance. You should choose serum separation gels with good hydrolysis resistance, strong stability, and radiation resistance that does not change its performance. The serum separation gels produced by Desheng are in line with these key indicators and are well recognized by the majority of customer groups. If you have relevant requirements for blood collection tube additives, you can click on the official website to consult the details.

In the rapidly developing field of medical technology today, in vitro diagnostic technology plays a crucial role. Among them, the use of blood anticoagulants is an indispensable part. Among numerous blood anticoagulants, EDTA K2 and EDTA K3 are highly favored in clinical applications due to their unique anticoagulant properties. So, what is the difference between these two common blood anticoagulants?   Firstly, from the appearance, both EDTA K2 and EDTA K3 present a pure white powder shape. However, upon careful observation, we will find that the powder of EDTA K3 is more delicate and has a better dissolution effect in water. Although this difference may not be apparent to the naked eye, it can have a significant impact on the experimental results during the experiment.   Secondly, from a chemical structure perspective, the main difference between EDTA K2 and EDTA K3 lies in the quantity of potassium ions. Specifically, EDTA K3 has one more potassium ion than EDTA K2, which leads to a significant difference in pH between the two. The pH value of EDTA K2 needs to be strictly controlled in a weakly acidic environment of 2.7-6.2 to ensure the stability of its anticoagulant effect. EDTA K3, on the other hand, needs to be used in weakly alkaline environments ranging from 6.2 to 10.2. This wider pH range makes EDTA K3 more adaptable in certain specific environments. In addition, due to the different amounts of potassium ions, the solubility of EDTA K3 is also greater than that of EDTA K2, which is particularly important in the preparation of anticoagulant solutions.   In terms of usage, there are also significant differences between EDTA K2 and EDTA K3. EDTA K2 has a wider range of applications, not only for anticoagulation in blood collection tubes, but also for the separation of complex metals and metal ions. In addition, EDTA K2 is often added to detergents, liquid soap, and shampoo to improve product performance. In contrast, the use of EDTA K3 is relatively simple, mainly used for blood anticoagulation. However, in practical applications, EDTA K3 may have certain limitations in terms of anticoagulant effect. When the concentration of EDTA is high, EDTA K3 is more likely to cause red blood cell contraction, thereby affecting the quality of blood samples. In addition, when blood samples are left for a period of time, EDTA K3 may also cause changes in cell size, further affecting the accuracy of experimental results.   There is also a significant difference between EDTA K2 and EDTA K3 in the use of blood collection vessels. EDTA K3 is liquid in the glass tube of the blood collection tube, while the plastic tube is the same as EDTA K2, which is in a spray dry state. This different morphology requires the use of different processes and equipment for the preparation of blood collection vessels, thereby increasing production costs.   Hubei Xindesheng Material Technology Co., Ltd., as a professional enterprise engaged in the production of blood collection reagents, has been deeply rooted in this field for 19 years. The company always adheres to the principle of "customer first" and is committed to providing customers with high-quality, stable and reliable products. In order to ensure product quality, the company has established a dedicated quality inspection department to strictly control and supervise the quality of all products. Once a problem is identified, the company will take immediate measures to resolve it and ensure that non-conforming products do not enter the market. It is precisely with this strict quality control and responsible attitude towards customers that Hubei Xindesheng Material Technology Co., Ltd. has won a good reputation and customer recognition in the market. If you have any needs or questions about blood anticoagulants, please visit the company's official website for consultation and understanding.

As an organic compound with a hydrophobic material, serum separation gel has good thixotropic properties. After on-machine centrifugation, serum and blood clots are quickly separated, and a jelly-like isolation layer is formed between serum and blood clots, thereby preventing their interaction. The storage time and original properties of blood samples are ensured by passing through the separation gel compartment within a certain period of time, thereby improving the accuracy of blood testing.   Serum Separation Gel Parameters: Original Ezhou City,Hubei Brand Desheng Transportation By air,by sea Appearance Gel Size 25kg/drum Stock In stock   So which blood collection tubes can use serum separation gel? Here are details for you. 1. Nucleic acid detection tube Collection, delivery and storage of venous blood samples for nucleic acid detection by adding EDTA and serum separation gel to nucleic acid detection tubes. For the DNA amplification detection of HIV, DNA, and HCV of HBV, serum separation gel has a good barrier to the interference of hemoglobin in red blood cells to nucleic acid detection experiments.   2. PRP tube The Chinese name is "high-concentration platelet plasma", which is mainly used to extract platelet-rich plasma through the precise specific gravity of the serum separation gel in the tube, and then inject the platelet-rich plasma into the required part of the human body to achieve the purpose of cosmetic repair, regeneration or treatment. Therefore, the PRP tube is not used to check whether there is a disease in the body, but to extract high-concentration platelets for reuse.   3. CPT tube CPT tube is also called vacuum mononuclear cell preparation tube. It is mainly added into the tube through serum separation gel of different specific gravity to separate lymphocytes and monocytes in blood. It is suitable for clinical medical testing and can be used to check HLA or residual Leukemia genetic testing, tuberculosis testing, HIV testing, etc.   4. PST tube The main purpose is to separate blood cells, serum and plasma, increase production, ensure the stability of plasma components, collect plasma samples, eliminate clotting time, and are mostly used in intensive care and emergency inspections.   Desheng Biochemical is a manufacturer specializing in the research, development and production of serum separation gels and ohter additives for blood collection tube. It has been developing for 17 years. Whether it is in the equipment environment, production team and product quality inspection, it has invested a lot of energy and time. The separation gel has also been improved by the first generation, the second generation and the third generation, until the fourth generation was developed and produced. Its performance and stability have made a qualitative leap. The PH value will not change, and the serum separation gel produced by Desheng has its own patented technology. Welcome to contact for any inquiry.

    Many female friends will use PRP when doing medical beauty, especially when treating dark circles, acupuncture, and wrinkle removal, but most people don't know much about PRP.         The full name of PRP in Chinese is high-concentration platelet plasma. It is a platelet-rich plasma obtained by extracting a certain amount of venous blood into a PRP tube, centrifuging and separating in vitro. It contains a variety of abundant growth factors, so it is widely used in medical beauty projects.         The PRP tube used in this process is a test tube specially used for the preparation of PRP plasma. Generally, serum separation gel is added to the test tube. This kind of separation gel has strict requirements on hydrolysis and specific gravity, and can control the precise specific gravity. Extract high-concentration platelets and inject them into the parts we need.         There are two kinds of separation gel routinely produced by Desheng, one is polyacrylic acid separation gel; the other is resin separation gel, both of which have obtained national patents. At present, the separation gel produced by our company has three appearances, transparent, translucent and opaque, with more obvious advantages and superior hydrophobic properties. The transparent gel is a unique appearance of our company. In terms of viscosity, the separation glue is customized, and few domestic manufacturers can provide such customized services.       With 17 years of development, Desheng's separation gel has developed into the fourth generation, from the previous generation that is not radiation resistant to the current resin system that is resistant to radiation, which is already ahead of most domestic manufacturers. Desheng has a professional R&D team for separating gel technology, with competitive prices and guaranteed quality. Now the daily output can reach 2-4 tons. The production technology and equipment are advanced, the delivery is timely, and the spot supply is guaranteed. If you have relevant needs, you can visit the official website or make a phone call.

In recent years, Good's buffer has been applied in more and more fields, and TAPS, as one of Good's buffer products, has gradually been paid attention to. TAPS is soluble in water to a zwitterionic buffer, with a pH buffer range of 7.7-9.1, and is often used in biochemistry and molecular biology.   Specific applications of TAPS TAPS can be used as a buffer component for DNA and RNA samples, and can protect oxyhemoglobin from reacting to methemoglobin in a freeze-dried environment. In addition, it can be used as a background electrolyte for micro-analysis of proteins in the field of capillary analysis, and can be used as an important pH stabilizer for biochemical diagnostic kits.   How to use TAPS The TAPS buffer produced by Desheng is in powder form,packed in barrels with a large large quantities.Then how to use it? First, according to your own needs, take out an appropriate amount of TAPS powder and dissolve it with deionized water. Be careful not to add too much water during the dissolution process, and ensure complete dissolution, and then continue to add water to make up the volume. Pay attention to the temperature when the volume is fixed. The temperature changes too much due to exothermic or endothermic dissolving, and the volume should be fixed when the temperature is close to room temperature. After configuration, seal the cap of the volumetric flask, which can be kept for future use. Mark the TAPS solution, as well as the concentration configuration, date and other information.   What are the advantages of Desheng's TAPS Desheng has been specializing in the development and production of in vitro diagnostic reagent raw materials for 17 years. As a veteran manufacturer, the TAPS buffer produced has a purity of 99% and stable performance. In addition, the company has specially set up a quality inspection department, and the various indicators of TAPS have reached the qualified standards, which can be used in experimental inspections with confidence and will not interfere with the inspection results.   At present, Desheng has a sufficient supply of biological buffers, no matter how big your needs are, the company can meet you, and can quickly prepare enough supply for you, package and send it out. In addition, the TAPS we produce is packed in powder, which is safer and more convenient to transport. Unlike the ready-to-use solution,which may lead to deformation or leakage, which will affect the experimental process. If you have any demands,click on the official website to consult the details.

Tripotassium ethylenediaminetetraacetate (EDTA-K3), as an amino polycarboxylate, has a strong ability to complex metal ions, and is often used as a chelating agent in hard water softeners to remove boiler scale and heavy metal mercury. Antidote etc. Tripotassium EDTA produced by Desheng Biochemical is mainly used as an anticoagulant in vitro. It is added to the vacuum blood collection tube. Generally, the pretreatment of blood samples is carried out in the process of clinical blood collection and testing.   The configuration of the EDTA tripotassium anticoagulant is to dissolve the EDTA tripotassium powder with a certain amount of distilled water, configure it into a solution of the required concentration, and then inject it into the blood collection tube. Compared with dipotassium EDTA, tripotassium EDTA is in the form of liquid in the blood collection glass tube, and in the form of dry spray in the blood collection plastic tube. When the tripotassium EDTA in the blood collection tube touches the blood sample, it complexes calcium ions in the blood, blocks the coagulation effect of calcium ions, and stops blood coagulation. Tripotassium EDTA, like dipotassium EDTA, also does not destroy blood cell components, does not affect the white blood cell count, and has the least effect on the shape of red blood cells, and is often used in routine blood tests. When tripotassium EDTA is added to the vacuum blood collection tube, it is usually used in combination with sodium fluoride and separating gel, and its anticoagulant effect is better. And in the case of non-irreversible anticoagulation with tripotassium EDTA, the test should be completed within 24 hours after blood collection to ensure the accuracy of the test results.   In addition, compared with sodium citrate and lithium heparin anticoagulants, tripotassium EDTA has a stronger ability to inhibit platelet aggregation. However, if EDTA tripotassium anticoagulation is used for blood analysis, it is found that the platelet count is too low for unknown reasons, and it is not suitable for the examination of platelet kinetics.   Hubei New Desheng is specialized in vacuum blood collection tube additives, not only R&D and production of tripotassium EDTA, but also serum separation gel, sodium heparin, lithium heparin, dipotassium EDTA, coagulant, high-efficiency coagulation accelerator Powder, etc., are conducive to meet the different needs of customers. This kind of blood collection tube additives, the purity is as high as 99%, and the quality is strictly controlled.

(4-Hydroxyethylpiperazineethanesulfonic acid) HEPES - the first choice for a variety of cosmetics (4-Hydroxyethylpiperazineethanesulfonic acid) HEPES is a type of biological buffer and is widely used in the market. It can control the system for a long time, and the pH value is stable, and it will not complex metal ions. It is often used in livestock feeding cryopreservation buffer solution, or used to make cell culture fluid. In addition, it can also be used in various cosmetics. It has good buffering capacity, mild and non-irritating, whitening and exfoliating. The role of HEPES in cosmetics 1. Whitening (4-Hydroxyethylpiperazine ethanesulfonic acid) HEPES contains weak acidity, which can destroy the desmosomes connected between keratinocytes on the skin barrier, promote the exfoliation of the stratum corneum, and make the skin smooth, delicate and smooth, whitening and translucent. effect. 2. Strong pH buffering ability ① The ingredients in many cosmetics are polypeptides, nucleic acid substances and fermented extracts. These substances often require a stable acid-base environment, and the pH value is in the range of 6.0-8.0, otherwise these functional ingredients are easily inactivated. While formulations are sometimes susceptible to oxidation during storage, resulting in a drop in pH, HEPES effectively stabilizes these ingredients within a reasonable pH range. ②The pH range of the microbial fermentation extract needs to be in the environment of 6.0-8.0 to maintain its activity, and the usual pH buffer system is not suitable. Phosphate radicals such as potassium dihydrogen phosphate/sodium buffer system will precipitate with metal cations in the extract and are not suitable for use. However, (4-hydroxyethylpiperazineethanesulfonic acid) HEPES has a pH buffer range of 6.8-8.2, and does not complex with metal cations such as magnesium ions and calcium ions, and has a pKa of 7.48 at ambient temperature of 25 °C. Ideal for microbial fermentation extracts to maintain activity. 3. Anti-inflammatory effect HEPES itself has a good anti-inflammatory effect, by reducing the amount of active oxidation products in peripheral blood mononuclear cells, it plays an anti-inflammatory effect and provides a new anti-inflammatory method. (4-Hydroxyethylpiperazineethanesulfonic acid) HEPES is widely used in cosmetics, such as polypeptide products, whitening and anti-aging products, exfoliating products, etc. As a supplier of HEPES, Desheng has officially passed the certification of cosmetic raw material submission code, which can meet different cosmetic customer groups. Feel free to contact us!

3-Morpholinepropanesulfonic acid is abbreviated as MOPS, CAS No. is 1132-61-2.It is a zwitterionic buffer commonly used in biochemistry and molecular biology, and its structure is similar to 2-(N-morpholine)ethanesulfonic acid (MES), also one of the "Good" buffers classified by Dr. Norman Good and colleagues in 1966. It has a wide range of applications, such as for mammalian cell culture (recommended concentration should be ≤20 mM), RNA denaturing agarose gel electrophoresis experiments (20 mM concentration is better for formaldehyde gels), discontinuous Polyacrylamide gel electrophoresis experiments, etc.   PH stability of 3-morpholinopropanesulfonic acid (MOPS) The PH stability of 3-morpholinepropanesulfonic acid and its effect on vascular tension in vitro were studied experimentally. The results showed that the PH changes of MOPS buffer and K+-MOPS solution within 14 days were basically constant at (7.32±0.04)～(7.51±0.04) 0.04). The aeration rate had little effect on the pH of MOPS solution, and the range of change was (7.24±0.04)～(7.39±0.00).While the Krebs solution was sensitive to the aeration speed, and the pH range was (6.81±0.81±0.00) between 15 and 60 bubbles/min. 0.07)～(8.33±0.02), the fluctuation range is large.   Changes in the volume of MOPS solution and Krebs solution under the same ventilation rate have little effect on their pH. The isolated rat mesenteric artery, cerebral basilar artery and coronary artery can produce good contraction in MOPS solution. Therefore the MOPS solution has the advantages of stable pH, long standing time, easy ventilation, and low application cost, and can be used in mammals experiments on isolated tissues and organs such as blood vessels.   Precautions for the use of 3-morpholinopropanesulfonic acid (MOPS) 1. MOPS can interfere with Folin protein analysis, and autoclaving of MOPS solution containing glucose will cause its partial inactivation. 2. MOPS powder can be stored at room temperature for several years. However, upon standing, the bulk powder may clump together and form hard lumps without affecting its chemical properties. 3. Storage method: MOPS powder can be stored stably at room temperature for several years. Prolonged storage may clump without affecting its use and chemical properties. 4. MOPS buffer can be stored at 2~8°C for at least 6 months, and sterilized by filtration with a 0.2 μm filter. Autoclaving is not recommended. To prepare a nuclease-free MOPS solution, autoclave the water before adding the powder to dissolve.   In the current market, Desheng is one of the most professional MOPS manufacturers in China, with a professional team for R&D and production. Desheng currently develops dozens of biological buffers, which have been traded in many regions around the world and are well received by the industry. If you are interested in MOPS or other buffer products, you can contact customer service for details.