China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The full name of EDTA-Na2 is disodium ethylenediaminetetraacetate, and it is usually used as a blood anticoagulant. Appearance is white or milky white granular powder, odorless and tasteless, soluble in water, but hardly soluble in ethanol. EDTA-Na2 is an excellent compounding agent in chemistry and a biochemical reagent with a very wide range of applications. However, in our daily life, when shopping in the supermarket, we sometimes find that there are ethylenediamine tetramine in the list of food ingredients. The name of disodium acetate is above. Don't worry about this. Although EDTA-Na2 is a chemical product, it mainly functions as a stabilizer, coagulant, antioxidant and preservative in food processing. In addition, the legal food additives specified in the "National Food Safety Standard Food Additive Use Standards", numbered GB 2760-2014, as long as the corresponding dose is used for the corresponding food category, it will not cause harm to the body. Many foods are protected by EDTA-Na2, and processed foods are treated with antioxidants to avoid deterioration, and can inhibit the proliferation of microorganisms and delay food spoilage, thereby "preserving the image." Therefore, without it, it would be difficult for us to enjoy all kinds of food at will. In addition to being used in food, EDTA-Na2 is also used in the medical field, among which blood anticoagulants are the main ones. It is an important complexing agent, which is mostly used in electroless copper plating, gold plating, aluminum-tin alloy plating, electrochemical polishing of steel parts and the solution before silver plating of copper parts. It can also be used in detergents, liquid soaps, shampoos, agricultural chemical sprays, color photosensitive materials processing bleaching fixers, water purifiers, PH regulators, etc. Here we recommend a manufacturer specializing in the production of blood collection tube additives: "Hubei Xindesheng Material Co., Ltd.". Desheng company produces EDTA-Na2 and also produces EDTA-K2/K3. The main core products are heparin series products and serum separation gel. Desheng company has a professional team to manage production and research and development. Up to now, Desheng company has produced blood collection reagents for more than ten years. Its product production experience is rich, the product quality is reliable, the production efficiency is good, the warehouse stock is sufficient, and the delivery is very good. rapid. If you are interested in Desun’s products, you can enter the company’s official website to contact customer service for more information.

Edta-2na is a white crystalline powder with CAS number of 6381-92-6. Its molecular formula is c10h14n2o8na2 · 2H2O. It is odorless, tasteless, soluble in water, and extremely difficult to dissolve in ethanol., As the most important chelating agent in industry, EDTA can be used as heavy metal detoxification agent, complexing agent, antioxidant synergist, stabilizer and softener; Calcium, magnesium, metal masking agents and other metal reagents. EDTA disodium is an important complexing agent, which is used for complexing metal ions and separating metal ions. It is commonly used in daily chemical and cleaning. How much do you know about the synthesis of edta-2na? In the following, a synthetic method of edta-2na is introduced. Firstly, the mixture of sodium cyanide and formaldehyde was slowly added into ethylenediamine aqueous solution according to a certain proportion, and air was introduced under 85 ℃ and reduced pressure to remove ammonia. After the reaction, adjust the pH value to 4.5 with concentrated sulfuric acid, and then decolorize, filter, concentrate, crystallize, separate and dry the product. Chloroacetic acid method: mix 100kg chloroacetic acid, 100kg ice and 135kg 30% NaOH solution, add 18kg 83% ～ 84% ethylenediamine under stirring, and keep at 15 ℃ for 1H. 30% NaOH solution was slowly added in batches until the reactants were alkaline and kept at room temperature for 12 h. Heating to 90 ℃, adding activated carbon to decolorize. The pH value of the filtrate was adjusted to 4.5 with hydrochloric acid, then concentrated and filtered at 90 ℃; The filtrate is cooled, crystallized, separated, washed and dried at 70 ℃. The reaction of EDTA and NaOH solution was used to prepare: 292g EDTA and 1.2L water were added into the 2l reaction flask with stirrer. Add 200 ml 30% sodium hydroxide solution under stirring and heat until all reactions are completed. Add 20% hydrochloric acid to neutralize to pH = 4.5, heat to 90 ℃, concentrate and filter. The filtrate crystallizes after cooling. The product was separated by suction filtration, washed with distilled water and dried at 70 ℃ to obtain disodium ethylenediaminetetraacetic acid dihydrate. Ethylenediaminetetraacetic acid and water are added into the enamel reaction tank, sodium hydroxide solution is added under stirring, heated to all reactions, hydrochloric acid is added to pH 4.5, heated to 90 ℃, concentrated, filtered, the filtrate is cooled, crystallized, washed and dried at 70 ℃ to obtain ethylenediaminetetraacetic acid disodium. Ethylenediamine tetraacetic acid was obtained by condensation of sodium chloroacetate with ethylenediamine, acidification and neutralization with sodium hydroxide. Dissolve the crude product in 10 times water, add equal volume ethanol to precipitate disodium salt, then filter and wash. Hubei xindesheng Material Co., Ltd. is a professional manufacturer of EDTA, which has been established for decades and has been engaged in the R & D and production of blood vessel additives. At present, the company has dozens of vascular additive products, including heparin series and EDTA series as the main core products. Interested in understanding can call consultation, Hubei xindesheng welcome your call.

Correct blood collection and timely treatment are the key pre analysis steps to ensure the integrity of blood testing laboratory results. EDTA, sodium citrate and potassium oxalate are important components of blood anticoagulants. Combined with the feedback from the end customers of EDTA, sodium citrate and potassium oxalate, we discuss again how the blood collection materials of EDTA, sodium citrate and potassium oxalate in the additive of blood collection tube (BCT) can change the chemical test results. 1) EDTA salt ethylenediamine tetraacetate EDTA can maintain glucose concentration by inhibiting glyceraldehyde-3-phosphate dehydrogenase. Considering that any kind of additive may change the stability of the analyte. As an anticoagulant and chelating agent, potassium EDTA can interfere with the determination of calcium and the production of blood clot, but it is preferred in hematological tests. As the upstream and downstream enterprises of blood vessel additives, manufacturers, sellers, clinical laboratory physicians and a series of practitioners must consider the pre analysis challenges in laboratory testing. EDTA and metal ions are immunoassay reagents, while zinc and magnesium are enzyme cofactors of immunoassay reagents, which bind to alkaline phosphatase. Insufficient sample size will lead to a relative increase in EDTA content, which will increase the chelation between magnesium and zinc, thus affecting the reagent enzymes used for signal generation, such as alkaline phosphatase. The reagent antibody recognized the binding site of divalent cation complex on protein; Therefore, the decrease of calcium and magnesium levels may lead to conformational changes, thus reducing antibody binding. 2) Citrate, disodium citrate, trisodium citrate, potassium citrate Sodium citrate condensation test preferably uses trisodium citrate in 3.2% (109 mmol / L) or 3.8% (129 mmol / L) solution. It can inhibit aspartate aminotransferase and alkaline phosphatase by cation chelation. Sodium citrate can inhibit platelet activation and measure the plasma level of platelet-derived components. Citrate buffer solution (pH < 5.9) can inhibit glycolysis immediately and keep glucose concentration. 3) Potassium oxalate Potassium oxalate, another calcium chelating anticoagulant, often used in combination with anti glycolytic agents (sodium fluoride and sodium iodoacetate), can actually reduce hematocrit by up to 10% by inhaling water from cells into plasma. Oxalate can also inhibit several enzymes, such as amylase, lactate dehydrogenase, acid and alkaline phosphatase. 4) Summary Desheng biochemical is a professional manufacturer of EDTA, sodium citrate and potassium oxalate. It has been deeply engaged in the industry for 15 years and is supported by a strong R & D team. Desheng biochemical suggested that employees should decide which anticoagulant to use according to the actual demand, so as to achieve the best detection effect.

Everyone has the love of beauty. When it comes to skin care, you have to mention aloe vera gel as the most versatile skin care product in the skin care industry. Aloe vera gel, the ingredients are extracted from a miraculous plant-aloe. Aloe vera juice contains a variety of nutrients and is widely used in daily life. However, the freshly extracted aloe vera has poor compatibility with the skin, fast volatilization and other characteristics, which are not suitable for direct application to the skin. It needs the assistance of another substance-carbomer. The two cannot be dissolved directly. Here is a preparation scheme of aloe vera gel and carbomer gel.     We need to use triethanolamine plus a little antibacterial agent to make the final aloe vera gel product can be stored longer. Put the carbomer powder into an empty jar and stir quickly until it is uniform. If there is agglomeration, use a stir bar to break it. Or just add half the amount of water, and add the rest before the antibacterial agent after the transparent gel is formed . Set aside and stir occasionally until the carbomer fully absorbs water and swells and no lumps are visible. It takes about 2 to 3 hours for the gel to fully expand and form. If you want to shorten the gel formation time, you can use the water-proof heating method, and stir while heating. It takes about 10 to 30 minutes.     Then add alkali to neutralize. Add slowly drop by drop, add and stir while measuring the pH value. At pH 7, it becomes a transparent gel. This step is very important and is the key to the success or failure of forming a transparent gel. When making the first time, it is best to add a drop of neutralizer-stir-measure the pH value, and repeat this step until the pH value reaches 7. This can be easily grasped When it comes to the preparation technology, the next step is very easy. Finally, add the antibacterial agent, stir evenly, pour into the extracted aloe vera gel, add glycerin, hyaluronic acid, polyglutamic acid and pure water to adjust the viscosity . In this way, the aloe vera gel is made.     Aloe gel itself is a very pure pure plant ingredient extracted from aloe. However, most of the transparent gel-like ingredients on the market are not aloe vera gel, but are made of water-added gels (carbomer, etc.). This kind of jelly-like, very transparent gel substance is actually made by adding a lot of water and chemical moisturizing ingredients: (Carbomer). According to different usage scenarios, there are 100% and 92% different components to choose from. When buying aloe vera gel products, you must pay attention to the ingredients and choose the one that suits you.     Desheng Biochemical is a high-tech enterprise focusing on the fields of life science and biotechnology. Based on the tenet of serving customers and serving scientific research, the company provides Chinese experts and scholars with a complete range of high-quality products. The carbomer, tris, Bicine, CAPS, MOPS, TAPS, HEPES, etc. produced have been widely used in life science basic research, development and application, biotechnology and many other fields.

Carbomer is officially the trade name of polyacrylic acid. As an intermediate material, it is usually sold to manufacturers in the form of dry carbomer white fluffy powder. It is often used in personal care products, such as gels, facial moisturizers and scrubs. They can prevent emulsion separation and control the consistency of makeup flow. . Carbomer is commonly used in no-rinse disinfectants. If you have seen the ingredient carbomer in the ingredient list of makeup products, you should have a basic concept. Is Carbomer safe for the skin? Many people will have questions in this regard. Don't worry, even if you are not sure what it is, please read it carefully many times, here is the complete information you want.   Carbomer has many code names, including 910, 934, 940, 941, and 934P. They indicate the molecular weight and the specific composition of the polymer. Carbomer 940 is widely used in hair styling gels, hydro-lactol gels, moisture-retaining gels, bath products, hand/body and facial lotions, etc.   The neutralized carbomer (polyacrylic acid) gel is suitable for obtaining biocompatible matrices for medical applications, such as gels used in skin care or dermatological treatment products. Carbomer films can be deposited on orthopedic implants to prevent corrosion. According to a human exposure study, 30 adult women were tested for skin irritation and sensitization with a skin moisturizer containing 0.20% Carbomer-934. 3 times per day, a total of 84 times in a row, apply the undiluted product to the face and eye circumference area. On the 14th and 28th days, the facial, conjunctiva, and mucous membranes were examined for residual irritation. The occlusive patch is applied before and after treatment. No skin irritation or allergy was observed in any test subjects. The researchers concluded: "Under normal expected use conditions, the sensitization and irritation of Carbomer to the skin is within the safety regulations.   Cancer is accompanied by the destruction of regular cells and does not undergo programmed cell death as fast as through mitosis. Carcinogens may increase the risk of cancer by altering cell metabolism or directly destroying DNA in cells. This can interfere with biological processes and cause uncontrolled malignant divisions, which ultimately leads to the formation of tumors.   The International Agency for Research on Cancer (IARC) is an intergovernmental agency established in 1965 and is affiliated with the United Nations World Health Organization. It is located in Lyon, France. Since the middle of the 20th century, it has published a series of monographs on human carcinogenic risk assessment. These monographs have had a great impact on the classification of possible carcinogens. There is no report that carbomer has a risk of causing cancer.   Desheng Biochemical Technology was founded in 2005, specializing in Carbomer (Carbomer 940/Carbomer 980 series), virus preservation solution, blood collection tube additive reagent, in vitro diagnostic additive reagent, biological buffer reagent, chemiluminescence Research and development, production and sales of substrate products. It constitutes a professional production and R&D capability with independent intellectual property rights. Provide products and biological raw material powder solutions for domestic partners and dozens of foreign manufacturers.

The continuous spread of the global epidemic has made the world panic. How to quickly and effectively isolate the high-risk positive populations and prevent the further spread of the epidemic has become a top priority. Anticoagulants commonly used in coagulation testing include EDTA, sodium heparin, lithium heparin, and sodium citrate. As a general routine detection method for new crown detection, the detection accuracy is higher than other methods, and the detection range is wider, especially for The advantages of screening for asymptomatic patients are very obvious. Although coagulation abnormalities have not been reported to cause infection, they will affect the accuracy and timeliness of coagulation test results.     Our comprehensive coagulation measurement product portfolio can help doctors make reasonable diagnosis and treatment decisions. In order to quickly, accurately identify and treat abnormalities in coagulation, we have increased the production of important coagulation reagents. Coagulation tests play an important role in the diagnosis, treatment and management of coagulation abnormalities in patients with new coronary disease. We have changed our production model to ensure increased reagent production, while also taking appropriate safety measures to help protect our workers from exposure to the new crown. We will continue these efforts to ensure that we can provide high-quality testing methods to meet the growing testing needs.     The new coronavirus is currently found to be related to an unprecedented blood coagulation-related disease in affected patients. From benign skin lesions on the feet to life-threatening thrombotic events, the new coronavirus has shown a high incidence of bleeding clots. Approximately 22% of new crown patients with coagulation abnormalities evolved into serious diseases. Depending on the severity of the patient's disease condition, anticoagulation treatment and monitoring recommendations may vary.     Some leading coagulation experts recommend the use of LMWH4 or UFH6 for therapeutic anticoagulation in patients with severe new coronary disease, and even recommend preventive anticoagulation for all hospitalized patients. The determination of antithrombin levels may be helpful because antithrombin can affect the therapeutic effect of heparin and may decrease in patients with new coronary disease.     As a leading technology company for blood collection tubes, biological buffers, and virus preservation solutions, Desheng Biotech is committed to the development and production of blood collection tube preparations, and indirectly provides accurate diagnostic services for medical testing. And all these changes are realized through continuous optimization of blood collection tube preparations. It is estimated that 500,000 patients across the country benefit from our diagnostic reagents every day. Including serum separation, coagulant and anticoagulant. Provide accurate and rapid diagnosis results, transform medical services and improve patient experience, thereby enhancing industry value.

Separating gel blood vessels have been widely used in clinical laboratories. Separating glue can form an isolation layer between cell components and serum (plasma), effectively prevent the exchange of substances between blood cells and serum (plasma), and ensure the stability of serum (plasma) components within a certain period of time.     There are generally two types of separation gel blood collection tubes commonly used in laboratories: serum separation gel coagulation tube and plasma separation gel anticoagulation tube. The serum separation gel coagulation tube is to add a coagulant to the blood collection tube to shorten the blood coagulation time, quickly get the serum, and report the result in the shortest time. The glass blood collection tube does not need to add coagulant, the blood contacting the glass tube wall will trigger coagulation. When xr and xn blood coagulation factors come into contact with plastic blood collection tubes, they are very weakly stimulated, and coagulants need to be added to shorten the clotting time. The plasma separator gel anticoagulant tube is sprayed with anticoagulant such as lithium heparin on the inner wall of the separator gel blood collection tube to meet the needs of rapid plasma biochemical emergency testing.     Separation gel is a thixotropic mucus colloid composed of hydrophobic organic compounds and silica powder. The structure contains a large number of hydrogen bonds. The existence of hydrogen bonds constitutes the chemical basis of separation gel thixotropy. Separation gel quality The specific gravity of separation gel is between serum (plasma) and blood cells, which is the physical basis for the reversibility of separation gel and the separation of serum (plasma). If the quality of the separation gel for blood collection tubes is poor, and the specific gravity cannot meet the requirements, it will inevitably affect the separation of serum (plasma), and the separation gel and serum (plasma) are likely to be intertwined.     Although the components of the separation gel are inert to the blood, there is no guarantee that it will have no effect on all manufacturers' detection systems and all items. There are many manufacturers of blood collection tubes, and each company has different components and ratios of the separation gel, and unique materials are mixed to maintain its stability. Therefore, the transparency, color and performance of the separation glue of each company are different.     Desheng is a long-established manufacturer of serum separator gels and has more than ten years of R&D and production experience. The separation glue produced by Desheng is mainly acrylic system and resin system. Three types of separation glues: transparent, translucent and opaque can be customized according to customer needs. The conventional packaging is 20kg/barrel and 25kg/barrel, and there are plastic barrels and iron barrels. , Other packaging can also be customized according to customer needs.

The production of trishydroxymethyl amino methane also needs attention to environmental protection   Now why few manufacturers produce tris, is not unwilling, nor is it lack of equipment, not understanding the procedures, but the requirements of environmental protection, air pollution, solid waste pollution, emission pollution, etc., production There are many such problems in the process of tris.     The living standards of modern people are getting higher and higher, and with the progress of the times, various resources are emerging in an endless stream. It can be said that basically all that can be enjoyed are enjoyed, but the environmental problems facing them are also more severe. In the past, the well water that could be directly drunk now needs to be filtered in many places; in the past, you can see the blue sky and white clouds when you look up, but now you can only see the thick white smoke in the distance. Under the premise that we cannot deny all of them, what we can do better is how to better restrain ourselves from causing some pollution to the environment when we produce various products.     Desheng Biochemical is a company specializing in biochemical reagents. It has been 15 years since its establishment in 2005. The production of Tris The R&D team conducts small and pilot tests, and the pollution during the production process is relatively small. But at this time, the company’s boss has clearly stated that it is necessary to think of a solution as soon as possible how to reduce environmental pollution in future large-scale trial production.     While being a company, we can also take into account the importance of the environment. This is not only responsible for the company, but also for the society and future generations. After the big test, Desheng constantly tried to use the smallest possible resources to obtain a greater yield. This is the use of resources, and it is also making a fuss on the emergence of wastewater to see if it can be recycled. Although every attempt is not necessarily successful, but dare to take into account the environment on the basis of cost growth, the tris-hydroxymethyl aminomethane produced by Desheng Biochemical has surpassed many manufacturers.     Recently, there has been certain fluctuations in tris hydroxymethylaminomethane, and the domestic supply is in short supply. Desheng Biochemical has been working overtime to ensure the normal production of various cooperative units. Friends in need can communicate and order in advance!

There is a way to make the luminol luminescence system enhance a lot     Luminol is also called luminol. Its CAS number is 521-31-3. Its appearance is a yellow powder. It is a chemiluminescent reagent. Chemiluminescence has the characteristics of high sensitivity, wide detection linear range and no pollution. It is an ideal immunoassay technique. There is a way to increase the luminescence of luminol a lot. In chemiimmunoluminescence analysis, the use of catalysts and luminescence enhancers can stabilize the chemiluminescence, increase the intensity and prolong the luminescence time. In addition, constructing a catalytic interface that can preferentially attract reactants is also one of the important strategies to enhance chemiluminescence.     It can be seen in the literature that academician Wang Erkang and associate researcher Li Jing of Changchun Institute of Applied Chemistry of the Chinese Academy of Sciences used in-situ synthesis to construct a flower-like cobalt hydroxide catalytic interface with a layered porous structure in an environment where BSA and Co2+ coexist. Effectively enhance chemiluminescence.     Cobalt hydroxide itself has unique physical and chemical properties, its layered structure and good electrochemical oxidation-reduction performance, has a wide range of applications in catalysis, electrochemistry, gas-phase sensors and haloacetic acid degradation, etc., and cobalt hydroxide is excellent The characteristics are affected by its morphology and size. This flower-like cobalt hydroxide catalytic interface with a layered porous structure has oxidase activity and a unique microstructure, which can effectively generate active oxygen.     At present, chemiluminescence immunoassay mainly includes luminol, isoluminol, acridine and cyclodioxane derivatives and other systems. The researchers tested the effect of this catalytic interface on the luminescence intensity of the luminol luminescence system mediated by BSA and Co2+ under alkaline conditions. The results show that compared with the traditional luminol-Co2+ or luminol-BSA system, the in-situ catalytic interface can achieve a 100-fold increase in chemiluminescence.     In addition, this catalytic enhancement mechanism can also be extended to different proteins, such as lysozyme, protamine, thrombin, and papain. The researchers also built a sensing platform that can measure ascorbic acid based on the quenching effect of chemiluminescence, and proved its good performance.     After the above, this research work provides a new way to design a catalytic interface that can enhance CL luminescence. It can help the chemiluminescence system to play a role in inorganic analysis, organic analysis, immunoassay, nucleic acid analysis and other fields. Hubei Xindesheng Materials also produces luminol, isoluminol, acridine esters and other chemiluminescence reagents. Among them, luminol has been researched and produced for many years. The product purity is ≥98% and the chemiluminescence efficiency is high. The products produced by Hubei Xindesheng Materials are of high quality and preferential prices. If you want to know, you can call to discuss in detail.

TRIS stands for Tris(hydroxymethyl)aminomethane, white crystalline particles in appearance, CAS number is 77-86-1. As the most commonly used raw material for buffer preparation, TRIS is widely used in biochemistry and molecular biology experiments. In addition, TRIS is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Because it has three equal hydroxyl groups, it can also be used as a good reducing agent. Under the alkaline condition of sodium hydroxide, the chloroauric acid solution can be reduced to prepare stable gold nanoparticles. This method is very environmentally friendly, non-toxic and harmless, and can be called green magic.     The more classic methods for synthesizing gold nanoparticles are sodium citrate reduction method, phase transfer method and seed growth method. These methods are very easy to modify surface biomolecules on gold nanoparticles, but the preparation and modification processes of these methods have certain toxicity. In order to reduce the toxicity of gold nanoparticles to biological organisms in experiments, and enable them to obtain better applications in the field of biomedicine, in recent years, scientific researchers have been committed to continuously developing new green reduction methods.     Under alkaline conditions, the chloroauric acid solution is reduced by ultrasound without adding other stabilizers, and relatively stable gold nanoparticles can be prepared. The synthesis of environmentally friendly and biocompatible gold nanoparticles is realized. By adjusting the experimental conditions, gold nanoparticles of different sizes can be prepared.     The preparation of gold nanoparticles by TRIS ultrasonic method is a new green and environmentally friendly reduction method. Compared with other green synthesis methods, the experimental conditions are milder and the operation is simple and easy. This provides new research ideas and theoretical basis for the synthesis of gold nanoparticles with green pollution-free, low toxicity, low cost, and high biocompatibility.     Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents, luminescent substrates and other products. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Did you choose the right sodium citrate for the pharmaceutical industry?   Sodium citrate is a widely used chemical additive in food, medical and chemical industries. Widely used in medicine and other industries. However, for some people who are new to this product, they are not clear about the requirements of sodium citrate in different application fields. For example, recently we have contacted a purchaser from a daily chemical product factory. The sodium citrate he needs and the trisodium citrate dihydrate we use as an anticoagulant in blood collection tubes are completely different products and cannot be replaced by each other.     Sodium citrate is usually referred to as trisodium citrate. The full name is actually called trisodium citrate dihydrate. The molecular formula is Na3C6H5O7.2H2O. This substance is widely used in food additives, emulsification enhancers and the pharmaceutical industry. Colorless crystals or white crystalline powders, taste salty, and have a unique cooling sensation. In addition to trisodium citrate dihydrate, there is also trisodium citrate pentahydrate with the molecular formula Na3C6H5O7·5.5H2O. The difference is only the number of crystal water.     Sodium citrate is often used as an anticoagulant in medical and health care. Sodium citrate can form difficult-to-dissociate complexes with calcium ions in the blood to prevent calcium ions from participating in the coagulation process. This is the main product of Desheng Biochemical Sodium Citrate field. Like EDTA Na2 and potassium oxalate, it is a good coagulant in blood collection tube additives. In clinical collection of fresh blood, it is necessary to add a certain amount of sterilized sodium citrate as an anticoagulant. The citric acid generated by the ionization of sodium citrate can combine with calcium ions in the blood to form complexes that are difficult to ionize, thereby inhibiting The coagulation of blood, so sodium citrate is called an anticoagulant. It can also be used as a diuretic and phlegm-reducing medicine.     Sodium citrate has many excellent properties: such as safety and non-toxicity, biodegradability, strong complexing power for metal ions, excellent solubility, and good pH adjustment ability. It should be noted in the pharmaceutical industry that citrate ions and calcium ions can form a soluble complex that is difficult to dissociate, thus reducing the concentration of calcium ions in the blood and hindering blood coagulation. This product is used as an in vitro anticoagulant during blood transfusion or laboratory blood sample anticoagulation. In the formation of prothrombin activator and the subsequent clotting process, calcium ions must be involved.     Desheng Biochemical has specialized in the production of sodium citrate since 2005. I would like to remind you that sodium citrate has a wide range of uses, including analytical grade, industrial grade and medical grade. According to the needs of special industries, special attention should be paid to the purity, the content of crystal water, and the packaging and storage methods. If you have any questions about the use of the product, please contact Desheng Biochemical for consultation.

What are the advantages and disadvantages of biological buffers   Biological buffer is a kind of solution that can keep the pH value of the solution relatively stable when adding a small amount of acid/base. The pH range of the biological buffer is determined by its pKa value. The pKa value is defined as the pH value when 50% of the molecules have an acid structure and 50% have a basic structure. A general principle about biological buffers is that the pH value should be within 1 pH unit around the pKa value, so as to ensure that it has a good buffering capacity. In this way, it can be ensured that there are enough molecules existing in the acid structure and the basic structure at the same time to neutralize them when H+ or OH- is added. In this way, the buffer can prevent changes in protein stability caused by changes in pH.     Many substances can be used in biological buffers. The frequently used buffer components usually have a pKa value close to neutral and can be used around the physiological pH range. The following will list the 3 most commonly used biological buffers, their respective pH application ranges, and their advantages and disadvantages that may affect the protein purification process. To ensure sufficient buffering capacity, the concentration of these buffers is usually 25mM.     1. MOPS buffer solution: PH range is 6.5-7.9, can not be autoclaved, PH value changes with temperature to a certain extent, and has high buffering capacity within the physiological PH value range.     2. HEPES buffer solution: PH range is 6.8-8.2, can not be autoclaved, PH value changes with temperature to a certain extent, and free radicals will be generated under certain conditions.     3. TRIS buffer: PH range is 7.5-9.0. PH value changes with temperature or degree of dilution to a certain extent, which will affect some enzyme activities. It has no ultraviolet absorption and is cheap.     The function of the buffer solution is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested meets the range specified by the analytical method. For example, phenol can be combined with 4-aminoamino acid in the presence of alkaline medium and potassium ferricyanide. Tipyrine reacts to produce orange-red antipyrine dye. In order to prevent the interference of aromatic amines, the most suitable pH is 10±0.2. For this reason, it is necessary to add ammonia-money chloride buffer solution to the solution to be tested to adjust and control the pH of the solution to be tested to 10.0±0.2.     Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents and luminescent substrates. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.