China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Hearing about the virus made us feel terrible. Especially last year's COVID-19, let the whole world smell it. What does this have to do with ourVirus Transport Media? As the name suggests, virus preservation liquid is the liquid to preserve virus. It is to prevent it from harming us, so that we can better understand, understand, study and analyze it, and then overcome it.   The Virus Transport Media is a liquid for sampling virus swabs in the tube to sample the swabs. It is mainly used for the collection, preservation and transportation of common virus samples such as New Coronavirus, hfmv, influenza virus and so on. It can be used to collect tissue samples from throat swabs, nasal swabs or specific parts. The stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification. It is usually divided into two types, one is non inactivated and the other is inactivated; The non inactivated type can protect virus protein and nucleic acid, preserve virus activity and facilitate culture and detection; The inactivated type makes the virus no longer have physiological activity, lose infection and pathogenicity, and have fecundity. It can inactivate the virus, eliminate secondary infection, and protect the safety of transportation and testing personnel.   How do we choose theVirus Transport Media? Since the virus is a parasitic organism, it cannot survive in vitro after sampling. If it cannot be detected in time, it needs to be stored in theVirus Transport Media. In order to protect the safety of the virus detection environment and the safety of detection personnel, we need to use our virus inactivation preservation solution.   But the inactivated preservation solution doesn't make the virus inactive. Can it be detected? In fact, the purpose of using inactivated preservation solution is to cleave nucleic acid, release nucleic acid, and then detect nucleic acid through follow-up real-time fluorescent RT-PCR, so as to judge whether the sample contains virus characteristic nucleic acid, that is, whether it is infected with virus. The virus has a single structure and contains only one nucleic acid and protein. Therefore, the virus is detected when the characteristic nucleic acid is detected.   Under what circumstances do we use non inactivatedVirus Transport Media? The non inactivated preservation solution is mainly an improved virus maintenance solution based on the transport medium. This one is to retain the protein shell of the virus and virus nucleic acid DNA or RNA at the same time, so that the virus has the integrity of protein antigen epitope and nucleic acid in vitro. Of course, there is a certain risk of infection in case of misoperation. Strict low temperature is required for long-term storage after sampling.   No matter what kind ofVirus Transport Media is used, not all samples can be transported and preserved at the sample collection place after virus sampling. Therefore, we need to transport and retain the collected virus swab, otherwise the virus nucleic acid will degrade quickly and the nucleic acid cannot be detected, Therefore, it is necessary to add preservation solution to the sampling tube to prevent the nucleic acid of the virus from being degraded so quickly. At the same time, after sampling, it shall be tested as soon as possible or strictly stored at low temperature to ensure that the detection is accurate.Virus Transport Media plays an important role in maintaining the stability of virus samples due to the rapid reproduction and variation of virus.

Luminol is also known as luminescent ammonia, and its English name is 5-Amino-2, 3-dihydro-1, 4-phthalazinedione. It is a yellow crystal or beige powder at room temperature. It is a relatively stable chemical reagent. Luminol reagent is a mixture of luminol and hydrogen peroxide, which is mainly used for blood detection in modern criminal investigation. Luminol reagent is a reagent to identify blood. Even if the blood is wiped, heme in the blood will remain. When luminol reagent is sprayed on heme, it will oxidize with reactive oxygen species and release blue purple fluorescence. Known as the luminol reaction. It is an organic substance used to identify blood.   However, luminol also has some shortcomings in criminal investigation. For example, if the crime scene is completely treated with bleach, the fluorescence emitted by luminol will strongly cover up the existence of any blood stains. Or the animal blood at the crime scene is confused with the test blood, which will affect the test results. In addition, luminol also reacts with animal excreta, which is almost the same as the light emitted from the blood. In addition, luminol needs to be used in a dark environment, and luminol's luminous time is limited, so it is necessary to make detection records when luminol is luminous. Although there are some factors affecting luminol at the criminal investigation site, there will be some countermeasures. For example, when bleach is mixed into the blood, we can adopt the drying method to dry the site for a few days, and the effect of bleach will be greatly reduced.   Luminol and acridine ester are commonly used chemiluminescent reagents. Luminol has been recognized to react with OH radical to cause luminescence, superoxide anion radical or singlet oxygen and H2O2 in alkaline medium Desheng biotechnology has been making chemiluminescent reagents for many years. Luminol is the representative of chemiluminescent reagents. Desheng biotechnology has developed and produced products for many years, which can fully meet the requirements of various manufacturers with reliable quality and favorable price. If you are interested, you can call to discuss in detail.

Carbomer 940 is a thickening agent and gel used widely in the daily chemical industry. It includes sterile disinfectant gel and carbomer. Moreover, the temperature has dropped rapidly recently, the epidemic has a tendency to rebound, and the demand for carbomer has also increased. What is the price now?   Carbomer 940 is commonly used in the carbomer series, because skin feels good, thickening, viscosity and transparency are very suitable for skin care products such as creams, wash hands and hand sanitizers. Although there are many manufacturers producing Carbomer, due to its various uses, the quality and grade of carbomer used in different products are different, so the price is also obviously different. Take Desheng carbomer 940 as the raw material of daily chemicals as an example. If it is a ton, the price is about 130 per kilogram. The same Carbomer, the same concentration of products with different gel transparency, the raw material prices are also different. The products with excellent thickening performance and high transparency also include carbomer of Lubrizol. However, because they are imported products, the price is relatively high, and they do not need to be purchased from retail agents, so the supply is not flexible enough.   Although there are many domestic producers of Carbomer, although the price is lower, there is still a certain gap between the quality of many products and those imported. Especially for high-end cosmetics, the cost of raw materials is not high, and the cost of carbomer is less. Therefore, the carbomer with better quality is preferred as the raw material of gel or cream. This is why before the epidemic, most manufacturers used imported carbomer raw materials, and the imported carbomer began to use domestic raw materials only when the epidemic was limited.   There are also some carbomer prices due to different grades. For example, in the field of material industry, the price of carbomer leak proof adhesive modified by decoration materials is much lower; In the pharmaceutical field, Carbomer used as pharmaceutical excipients has higher grade and higher price than that used in cosmetics.   It can be seen that the same is carbomer 940. The price difference is relatively large for different grades and quality. Desheng's carbomer 940 is used for cosmetics and pharmaceutical accessories.

According to the feedback from the market to Carbomer, the transparency of carbomer has always been concerned by the majority of customers. A lot of carbomer powder can be made when it is powdered, but when it is dissolved, you will find that the gel penetration is not enough enough to meet the requirement.   Due to the special structure of Carbomer, it is easy to expand with water, and it has strong hydrophilicity. The carbomer hygroscopicity of dry powder is very strong, which is the same as other hygroscopic powders. Therefore, when it is put into water or other polar solvents by improper methods, it is easy to form clusters or incomplete wetting. In this way, the gel will appear granular and transparent. In addition, in some gel products, such as disinfectant and free gel, in order to increase permeability, anticorrosion and solubilization, ethanol is often added as an additive. The content can be as high as 75%. Carbomer gel is essentially a polymer aqueous solution. The stability of polymer solution is due to the existence of hydration film on the surface of the particle. The addition of strong hydrophilic non electrolytes (such as ethanol) will make the polymer solution flocculate. The carbomer ethanol gel prepared by different operation processes has different concentration of ethanol, and when the concentration of ethanol is too high, The finished gel will produce a little milky light and reduce the transparency of the gel. Therefore, if 2% of the purified water is added to the gel product with 70% ethanol content, the milk light will be significantly reduced compared with the finished products. This method has a certain effect on improving the appearance transparency of the finished products.   The big difference between Desheng carbomer and ordinary carbomer on the market is that in the powder state, it can be seen that the powder of Desheng carbomer is obviously white, and the powder is more fluffy and delicate. Moreover, the transparency of our carbomer is much higher than that of similar products. You can see the opposite sky through our kapom! At the same time, because we are a direct manufacturer, we can also customize individual indicators.   Whether it is used directly by customers or purchased by traders, it is a very good choice. You can purchase in small quantities or sign annual contracts to enjoy ultra-low discounts. While the product quality is guaranteed, the price is unlimited. Enjoy after-sales service. You can apply for direct return or refund for product quality problems. The choice of real manufacturers and reliable products is very important!

Acridine chemiluminescence reagents mainly include acridine ester and acridine sulfonamide. Acridine ester is a very important chemiluminescence reagent with high quantum yield. The chemiluminescence efficiency of acridine ester is relatively high, which is generally five times or more than five times that of luminol. In addition, the chemiluminescence process of acridine ester reacts rapidly and has low background. It can emit light in the presence of sodium hydroxide and hydrogen peroxide. In the process of oxidation reaction, the conjugate is decomposed and does not affect the luminescence of free acridine ester. In addition, the chemiluminescence reagents of acridine ester series have very good stability and convenient storage.   In recent years, with the development of chemiluminescence application technology and instruments, especially the expanded application of acridine ester series products, it has a very significant effect. There are many types of acridine esters, such as acridine ester dmae-nhs. This paper will introduce the dmae-nhs of acridine ester. At present, there are many processes for the synthesis of acridine ester dmae-nhs, which can be attributed to the seven step method. For the shortcomings of this method, such as difficult reaction steps, high cost of raw materials and reagents, which is not conducive to large-scale preparation, the traditional seven step method is optimized, and a new five step chemical reaction method for the synthesis of acridine salt is developed. Acridine ester dmae-nhs was synthesized from 3,5-dimethyl-4-hydroxybenzoic acid and acridine 9 carboxylic acid through five steps of esterification, condensation, hydrolysis, succinyl amination and methylation.   Based on the above understanding, the synthetic method and evolutionary history of acridine salt dmae-nhs. In Hubei xindesheng Material Technology Co., Ltd., acridine ester has been produced and developed for more than ten years. Among them, acridine ester series has many models: acridine ester (dmae-nhs, nsp-dmae-nhs, me-dmae-nhs), acridine salt (nsp-sa, nsp-sa-nhs), acridine hydrazide nsp-sa-adh and other products. In addition, Hubei xindesheng materials can also provide blood collection additives, biological buffers, enzyme preparations, Carbomer, virus preservation solution and other products. If you want to know about the products, you can call for consultation. Desheng welcomes your call.

Heparin lithium heparin sodium is a common additive for heparin tubes. This year, customers who purchased heparin lithium reported some problems to our company. For example, the doctor used heparin tube to operate blood for electrolyte analysis, and found that blood potassium and blood sodium were on the high side. Why? After receiving the customer's feedback, we first conducted repeated tests on the same batch of heparin lithium produced by our company to determine that its various indicators are normal. We plan to explore the answer from two aspects. One is to find the implementation conclusion of experts on the Internet and explore the answer from the professional perspective of doctors. 10g heparin lithium package per bottle The problem was found according to the medical and clinical literature. The effect of heparin lithium on the determination of electrolyte was understood by comparing the results of plasma anticoagulated with heparin lithium and serum without anticoagulant. The method used is to select two samples of different additives of the same object at the same time, a total of 40 samples for comparison. The results showed that heparin lithium anticoagulant had a significant effect on the determination of blood potassium, but there was no significant difference in the determination of blood sodium and blood chlorine. The experimental conclusion is that heparin lithium has an effect on the determination of blood potassium. It is suggested that non anticoagulant samples should be used for the determination of electrolyte.   The results showed that compared with serum without anticoagulant, heparin had no effect on the determination of blood sodium and blood chlorine, but there was a difference in blood potassium. Heparin lithium anticoagulant has a significant effect on the level of potassium in electrolyte determination. From the cause analysis, it is probably that heparin and potassium generate heparin potassium, which affects the determination of potassium by ion selective electrode, resulting in low blood potassium. At the same time, for serum samples, due to the destruction of platelets in the process of blood coagulation, the potassium ion in platelets (its concentration is much higher than plasma potassium) releases human blood, making the serum higher than plasma potassium, The increase was positively correlated with the number of platelets.   Serum potassium heparin lithium can not accurately reflect the real level of potassium in the body due to the fragmentation of platelets in the real process of evacuating serum after blood coagulation, so it is initiated to use plasma potassium instead of serum potassium. According to the same common clinical experience, although there is the effect of platelet destruction, the determination of serum potassium is still due to the determination of plasma potassium. The tension is that serum potassium has no effect of heparin, and serum is widely accepted as a sample in the old biochemical examination and analysis in China. From the perspective of the experience of professional doctors, this conclusion is also created. Heparin products represented by heparin lithium and heparin sodium have significant anticoagulant performance after blood collection. Whole blood cell count and blood cell examination are often used. Desheng biochemical is deeply engaged in the field of vascular preparations, and has more than 20 independently developed patented technologies. Ensure that the products obtained by customers are better than the average level of peers and enjoy high-quality after-sales technical service.

The chemiluminescence labeling method represented by acridine ester and luminol is the preferred scheme for the quantitative detection of ligand conjugates. These assay markets have a high demand for technology, which requires detection technology to be highly sensitive, less susceptible to interference and easy to use. The chemiluminescence process has sufficient sensitivity, so it is very suitable for these applications. Old fashioned assays require the separation of bound markers from unbound markers. Although the assay is easier to develop, it is somewhat complex to implement. On the contrary, the chemiluminescence labeling method represented by acridine ester adopts homogeneous determination, which affects the luminescence reaction in some way through the combination of ligand and its receptor, which is easy to observe and the dose required for the reaction is small. Chemiluminescence is a series of radioisotope based analytical procedures. Its feasibility is proved to be applicable to various analytes, including high molecular weight and low molecular weight substances. The stability and analytical performance of reagents are of great significance to improve and maintain high-quality analytical performance. Most importantly, the development of ICMA technology based on labeled antibodies has the potential to increase the sensitivity of determination. This technique may prove valuable in testing and monitoring infectious agents and tumor markers. We have done experiments in which the natural luminol reaction product is incorporated into globulin, and the luminescence of its derivatives is 100 times that of diazonium salt. This also confirms the potential of Lumino.   Chemiluminescence of luminol and acridine ester occurs under alkaline conditions, although in the case of luminol, there are catalyst requirements. The catalyst can be a simple transition metal cation, such as Mn + + or Ni, but it can also be complex macromolecules, such as horseradish peroxidase and cytochrome. The most popular catalyst is micro peroxidase because of this efficient reaction. Under relatively mild conditions. This requirement for catalysts makes the reaction vulnerable to the interference of substances in biological samples and powerful.   Desheng bio produces chemiluminescent reagents, such as luminol, isoluminol, acridine ester, etc. In addition, it also independently develops and produces dipotassium and Tripotassium EDTA, with high purity, content of more than 99.5%, good anticoagulant effect and no impurities. After detection by SGS inspection organization, the content of trace elements is one order of magnitude lower than the national and industrial standards (10 times), with good clarity and high solubility. 80 grams of dipotassium and Tripotassium can be quickly dissolved per 100ml of water at 25 ℃, It can maintain the original properties of blood to the greatest extent and has no significant impact on the blood clinical test results. It has been favored and affirmed by well-known enterprises at home and abroad, including Yangpu!

Tris industrial grade, analytical grade, pharmaceutical grade, conventional packaging 25kg/barrel, white crystal powder, purity>99%, small packaging can reach 500g/bottle, trial packaging 50-100g/bottle, purity difference 99% -99.99% can be customized. Uses of Tris(hydroxymethyl)aminomethane: commonly used in biological buffer preparation, biochemistry and molecular biology experiments, etc., surfactants, PH regulators in cosmetics, vulcanization accelerators and intermediates of fosfomycin, etc. The range of use can be It is said to be one of the more extensive biological buffers. Natural demand is also relatively high. There are not many tris manufacturers in the country, because tris is very dangerous in the production process. The step of hydrogenation has made many enterprises and manufacturers daunting, and manufacturers producing tris products have encountered safety accidents one after another. , It also indicates to the chemical industry that the product is not easy to come from, so since 2018, the price of tris has been rising. Desheng Biochemical is a manufacturer specializing in R&D and production of tris. Now it has supplied more than 100 companies to form stable sales and exported to many countries. With a monthly output of 20T, stable supply of goods, superior quality, perfect after-sales service, and professional technical guidance, all of these allow Desheng Tris BASE to enter the market faster. Whether you are using it for your own use or doing trade export, Desheng Biochemical can meet your needs very well. Free shipping nationwide, covering 34 provinces and cities across the country, and provide full service. Another advantage of the manufacturer’s direct supply of tris is that the product price will be lower, and Desheng recently launched a year-end discount event, the order price is lower than that on the market, business friends who need this product should not miss it!

In an environment where the new crown virus is raging around the world, it is self-evident that how to quickly check and isolate positive infections can play a role in controlling the epidemic. In the current popular specimen collection and processing methods, EDTA, Tris, and Virus Transport Medias play an important role in different stages. Here is a brief review.   Nasopharyngeal swab: The sampler gently holds the person's head with one hand, inserts the swab into the other, inserts the swab through the nostril to enter, and slowly becomes deeper along the bottom of the lower bridge of the nose. Because the nasal tube is curved, do not use excessive force to avoid traumatic bleeding. When does the tip of the swab reach the back wall of the nasopharyngeal cavity, gently rotate it once (if a reflex cough occurs, please wait a while), then slowly take out the swab and dip the tip of the swab into the 2-3ml virus Test tube of preservation solution (or isotonic saline solution, tissue culture solution).   Stool specimen: Take 1ml of sample treatment solution and take a sample of approximately one size. The stool specimen treatment solution can be prepared in the laboratory: 1.211g tris, 8.5g sodium chloride, 1.1g anhydrous calcium chloride or 1.47g water containing crystals of calcium chloride, dissolved in 800ml deionized water. Centrifuge at 8,000 rpm for 5 minutes, absorb the supernatant and save it for use.   Anal swab: Gently insert a sterile cotton swab into the anus to a depth of 3-5 cm, then gently rotate and pull it out, immediately put the swab into a 15ml screw cap sampling tube containing 3-5ml Virus Transport Media, discard the tail and Tighten the tube cap.   Blood sample: It is recommended to use a vacuum blood vessel containing EDTA anticoagulant to collect 5ml blood sample. Nucleic acid extraction should be carried out in whole blood or plasma according to the type of nucleic acid extraction reagent. For plasma separation, the whole blood should be centrifuged at 1,500 to 2,000 rpm for 10 minutes, and then the supernatant should be collected in a sterile plastic tube with a screw cap.   Serum specimen: Collect 5 ml blood specimen collection tube with vacuum negative pressure blood. Place the specimen at room temperature for 30 minutes, centrifuge at 1,500-2,000 rpm for 10 minutes, and collect the serum in a sterile plastic tube with a screw cap. Other materials: Collect in a standardized way according to design requirements.

Under normal conditions, serum separation gel is used in extracorporeal blood testing, but many people do not know whether serum separation gel is harmful to the human body. In fact, it can be judged from several aspects to determine whether it is harmful to the human body.    First of all, we can first look at the components of the serum separation gel. The components of the serum separation gel are polyolefin, polyester or propylene, silica powder, thixotropic agents and stabilizers. After these materials are in contact with blood, the product's hydrolysis resistance is not harmful, but the ability to resist hydrolysis will have a certain impact on the contact materials. However, nowadays, serum separation gels with better hydrolysis resistance are generally used. Another aspect is to look at the use status of the serum separation gel. Everyone knows that the earliest serum separation gel is generally used in vacuum blood collection tubes to separate serum and blood clots. With time development, the current serum separation gel can also be used in vacuum blood collection tubes. Plastic surgery and medical treatment in some departments are targeted, but this is by extracting substances from the human body, and then using the extracted substances to backfill the human body. Used in blood collection tubes, it still has no effect because it will not touch the human body. But will the use for cosmetic surgery affect the human body? Because this method of use is different from the blood collection tube, it is separated by the serum separation gel, so that the platelets and serum proteins in the serum and blood clots can be better extracted, but the serum separation gel will come into contact with the extract during this process. Yes, and these substances have to be filled back into the human body at the end, so does this part of the substances have an impact on the human body? This type of separation gel has a professional name called PRP gel. It is a serum separation gel that is specially used to extract platelets and other components. The quality specifications are very high. Only when the quality requirements and biocompatibility meet the requirements will it not be used. Have a negative impact on the human body.   It can be seen from the above that the serum separation gel basically has no effect on the human body. However, if you find that there are foreign bodies, abnormal odors, abnormal colors, etc. in the serum separation gel, or the effective use period has passed, try not to use it, because there is no guarantee whether it will affect the human body. Moreover, there are some differences in the process and raw materials between the manufacturers of serum separation gel. If there are multiple brands of serum separation gel, please do not mix them. This will cause the residue of the serum separation gel and affect the test results of the blood collection tube. Phenomena such as reaction of the contact material.

Today we will introduce TRIS, HEPES, TAPS, the team products in the Good’s buffer produced by Desheng.   TRIS (Trishydroxymethylaminomethane): Weakly alkaline, with a pKa of 8.1 at 25°C, and the effective buffer range of its buffer is between pH 7.0 and 9.2. It is often used as a buffer in electrophoresis and gel experiments, and is widely used as a solvent for nucleic acids and proteins. It can also be used for the formation of nematode intermediate fibers. The most common role in cosmetics is to adjust the PH value (pH). It can also be used as a neutralizer to neutralize a thickener (carbomer) to reduce stickiness, improve skin cell respiration efficiency, and prevent pore clogging . In addition, TRIS can be used as an odor regulator in cosmetics containing amine salts to adjust the amine odor generated by friction when applying cosmetics, so as to avoid any residual or sustained odor release. HEPES (4-hydroxyethylpiperazine ethanesulfonic acid): It is a zwitterionic biological buffer, belonging to Good’s buffer, with an effective buffer range of 6.8~8.2. It can be widely used in a variety of biochemical reactions and used as a buffer reagent in certain cell culture media. In addition, due to its non-cytotoxicity, HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. TAPS (Trimethylol methylamino propane sulfonic acid): It is also a zwitterionic buffer. In biochemical experiment projects, it can be used as an important pH stabilizing reagent. It is generally mixed with weak acid and its conjugate base to obtain an appropriate pH value. TAPS reacts with most organisms under neutral conditions. The pH value is required to be selected in the range of 6 to 8. At the same time, the effective buffer range of the buffer should also be specified in the range of 6-8. Moreover, it is necessary to ensure that the pH form of the buffer reagent cannot chelate with certain metal chemical ions. In the field of clinical diagnosis, TAPS is also commonly used as a biological buffer. Usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA/RNA extraction kits and other kits.

In our nucleic acid examination, we will use our company's product: Virus Transport Media. In fact, the Virus Transport Media is also divided into multiple models, and there is also a twin brother: cell preservation solution.   Among them, the Virus Transport Media is roughly divided into two types: inactivated type and non-inactivated type. It can be understood from the literal meaning that there are differences in the preservation principles of the two types of Virus Transport Medias. Here is a brief introduction. The first non-inactivated type can protect the protein and nucleic acid of the virus, and the other is the inactivated type, which usually contains the lysis salt of the inactivated virus, which cleavages the protein to protect the nucleic acid. Among them, if the non-inactivated Virus Transport Media cannot be tested in time after sampling, it must be stored below 4 degrees Celsius and the preservation time should not exceed 48 hours. If it cannot be delivered to the test site within time, it needs to be Store in ultra-low temperature below minus 70 degrees Celsius. Otherwise, it will affect the virus test results, so you need to select the type of Virus Transport Media corresponding to the situation when selecting the Virus Transport Media. Many people confuse the Virus Transport Media with the cell preservation solution. In fact, the cell preservation solution is a general-purpose cell cryopreservation solution that can be used to preserve animal and human cell lines. Cell preservation is a cytology experiment. Important technical means. In cell line establishment and line establishment, it is very important to freeze the original cells in time.   At present, cell preservation mostly uses glycerol or dimethyl sulfoxide protective agent, which can improve the permeability of the cell membrane to water, and then slow freezing can make the water in the cell seep out of the cell, reduce the formation of ice crystals in the cell, thereby reducing Cell damage caused by ice crystals. The cell preservation solution has a diluting effect, which can isolate a large number of effective cells embedded in the mucus, allowing more valuable cells to be preserved, providing a sufficient number of cells, and providing a guarantee for the accuracy of the test results.   Therefore, although the purpose of the Virus Transport Media and the cell preservation solution are similar, there are some differences in principle. Virus Transport Media is preserved by protecting nucleic acid, while cell preservation solution is preserved by improving cell capacity and protection. These two preservation solutions should be able to distinguish between them after the above understanding.