China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Tris(hydroxymethyl)aminomethane is a synthetic biological buffer for skin care and cosmetics. It can be used as a pH buffer regulator and fragrance ingredient. Tris(hydroxymethyl)aminomethane, also known as tris, is an organic amine proton acceptor. It is commonly used as a component of buffer solutions in biochemistry and molecular biology. Basically, the role of tris(hydroxymethyl)aminomethane is to improve stability and efficacy by adjusting the pH or acidity of the product. Tris is also used in the synthesis of surfactants and drugs, as well as mineral oil and paraffin emulsions, leather dressings, and special textile products as polish cleaners and emulsifiers. Advantages: Tris can improve stability and efficacy by adjusting the pH or acidity of the product. Tris is considered safe for its intended use and is not irritating or allergic to the skin.   What is the effect of tris(hydroxymethyl)aminomethane? Why does it appear in cosmetics and skin care products? The FDA reported the use of butyl triamine in 488 disposable products and 70 disposable products. It is used in disposable nail polishes and lotions as high as 3.7%. Other products include eye makeup up to 2%, fragrance formulations up to 0.2% and formulations up to 3.1%. Tris(hydroxymethyl)aminomethane can be used to establish and maintain the pH of the product. pH stands for "potential hydrogen" and it refers to the level of acidity or alkalinity in a given solution. The pH value ranges from 0 to 14. A pH of 7 is neutral. A pH of less than 7 is acidic. A pH greater than 7 is alkaline. The effective buffer range of tris(hydroxymethyl)aminomethane is 7 to 9. The normal pH of the skin is weakly acidic, usually between 4.7 and 5.75. Sebaceous glands maintain sweat glands and normal skin flora. The acid film provides a layer of amino/lactic acid and oil film, which can effectively protect the skin from environmental factors (such as bacteria, pollutants and water loss), which can cause premature aging and irritation. The pH of cosmetics and skin care products is important to maintain the normal pH of the skin as much as possible. If the product is too sour, it may irritate the skin or cause a tingling sensation. Products that are too alkaline are harmful because they deplete important natural fats or lipids in the skin. The cracked acid skin layer will also not allow the product to be absorbed into the skin. This is why pH adjusters (such as tris(hydroxymethyl)aminomethane) can be used in cosmetic formulations. Desheng Biochemical Technology Co., Ltd. specializes in the production and research of tris and carbomer. These are the main components of daily chemical products, which are basically used in the daily chemical industry. At the same time, it is also chemical as a PH regulator in organic synthesis. Common preparations for reactions. The company has a R&D team with 2 doctoral students and 8 masters, providing Tris and Carbomer R&D services for related industries.

Enzymes are highly specialized and complex proteins that contribute to the chemical changes in various parts of the body and exist in every organ and cell in the body. Such as L-homocysteine ​​methyltransferase, S-adenosine homocysteine ​​hydrolase (SAHH), S-adenosylmethionine synthetase, N-acetylneuraminic acid aldolase, fructose amino acid Oxidase, neuraminidase/sialidase, and NRH are all frequently heard words in clinical diagnosis. At the same time enzymes are necessary for your body to function properly.     (1) Enzymes in disease diagnosis Enzyme diagnosis is a method of diagnosing diseases by measuring the content and changes of certain substances in the body, or by the changes in the activity of the original enzymes in the body. Enzyme diagnostic methods are based on two aspects: changes in the original enzyme activity in the body and changes in certain substances in body fluids. These changes can be detected by enzymes. Phosphatases are hydrolases that catalyze the hydrolysis of phosphate monoesters into inorganic phosphate esters under acidic conditions. Patients with prostate cancer or hyperparathyroidism have elevated serum acid phosphatase activity. Another example: glucose oxidase can be used to detect glucose content in the diagnosis of diabetes; urease can be used to measure urea content to diagnose liver and kidney disease; cholesterol oxidase can be used to measure blood cholesterol content to quickly diagnose hyperlipidemia; Glutaminase can be used to measure the glutamine content in cerebrospinal fluid. DNA polymerase can be used to detect whether the gene is normal or whether there are any oncogenes in the body. It has unique enzymatic characteristics such as high catalytic efficiency and mild action conditions, and enzymatic diagnosis has become a reliable and rapid diagnostic method. (2) Enzymes in disease prevention and treatment You may not know that enzymes can be used as drugs to treat many diseases. Common trypsin can be used not only to accelerate wound healing and dissolve blood clots, but also to remove necrotic tissue and inhibit the proliferation of contaminating microorganisms. R-asparaginase can treat cancer by depriving cancer cells of nutrients needed for growth, and proteases (such as multi-enzyme tablets) can be used to treat indigestion and have anti-inflammatory effects. R-asparaginase, soybean meal fibrinolytic enzyme, thrombin, etc. can be used to treat diseases. Many enzymes are used as diagnostic reagents in medical treatments. Because of its remarkable curative effect and insignificant side effects, medicinal enzymes have become more and more widely used in the health field. Desheng Biochemical has been engaged in the production of enzyme substrate enzyme preparations for many years. Some products are for reference, adenosine deaminase, malate dehydrogenase, cystathionine β lyase (CBL), cystathionine β synthase (CBS), Betaine homocysteine ​​S-methyltransferase, creatinase/sarcosinase, creatinase

Today, the environmental ecology around us is deteriorating day by day. Constant smog and dust particles in the air will cause the eyes to dry out continuously. The pharmacy has a variety of facial care products, and its main ingredient contains one called carbomer. Before using carbomer, you need to read the carbomer instructions carefully and understand its meaning.   In the picture, the experimenter uses NaOH to convert the white powder into a transparent gel. 1) Pharmacological properties Carbomer can interact with mucin on the stratum corneum. The product is produced in the form of a colorless powder. The drug penetrates into the stratum corneum epithelium, and hydrogen bonds are generated due to carboxylic acid residues, and there is a viscous protein in it. The main advantage of this product is its adhesion in the stratum corneum. A protective layer is formed, which wets the stratum corneum and strengthens the mucin layer. Carbomers are macromolecules that contain compounds or monomers. The main advantage is the absorption and retention of water, their volume will change and reach a large size. 2) Carbomer in external skin products Carbomer can be seen in skin care products, such as foot care; toothpaste and sunscreen cosmetics. Carbomer also has the following advantages: forming a moisturizing film; sticky and non-toxic. It is not mutagenic and teratogenic, which has been confirmed by long-term testing. Carbomer does not accumulate and does not penetrate into the eyeballs and blood. Before using the thickener, you first need to neutralize it. It is possible to obtain a sticky consistency. Neutralization forms a molecular network that retains moisture. When diluted with liquid, the powder turns into a gel and becomes transparent. Use NaOH or potassium hydroxide to convert the powder into a gel. 3) Carbomer in cosmetics Carbomer is used as a thickening regulator in cosmetology. It is most commonly added to pastes, creams, gels, and bath products. It is widely used to make decorative cosmetics for the eyes. Carbomer is present in the following formulations: the raw material carbomer is used in powder form. After being diluted with liquid, it becomes a viscous emulsion and is used as a thickening agent. During the dilution, the substance will not lose its properties and useful qualities. The main advantage of this cosmetic is moisturizing. Carbomer-based creams refresh and soothe the skin without creating an oily film. 4) Where to buy Desheng Biochemical Technology Co., Ltd. is a professional carbomer manufacturer, which has been supplying mainstream carbomer 940/980 series for many years, and supports customized formulations to produce carbomer raw materials for different application environments. Collaborate to develop a carbomer suitable for specific scenarios.

Carbomer English name: Carbomer, white powder in appearance, good water solubility, mostly used in the daily chemical industry. Carbomer is a high molecular polymer of acrylic acid bonded allyl sucrose or pentaerythritol allyl ether. It is an acrylic cross-linked resin obtained by cross-linking pentaerythritol and acrylic acid, and belongs to a particularly important rheology modifier. Carbomer after neutralization is a good gel matrix, which has important purposes of thickening and suspending. Because of its simple operation and good stability, it is widely used in daily chemical products. Carbomer 940 and Carbomer 941 are more commonly used in daily chemical products. What is the difference between these two carbomers? 1. Carbomer 940: short rheology, high viscosity, high clarity, low ion resistance and shear resistance, mostly used in gels and creams. 2. Carbomer 941: Long rheology, low viscosity, high clarity, medium ion resistance and shear resistance, mostly used in gels and emulsions.   Carbomer 940 and Carbomer 941 are different in rheology, viscosity and ion resistance, and these factors affect the shape and viscosity of the finished product after processing. There is not much difference in overall usage, so many people will confuse them. And in the current market, there are still some merchants using Carbomer 940 or Carbomer 941 for mixed sales, which may affect customers' products. Here we recommend a manufacturer specializing in the production of carbomer: Hubei Xindesheng Material Co., Ltd., which has been producing chemical raw materials for decades. Desheng company has a professional team responsible for the R&D and production of Carbomer, and has imported equipment and raw materials for production and processing. The products produced by Desheng Company are of high quality, fast production efficiency, and sufficient stock in the warehouse. If you are interested in Desheng's products, you can enter the company's official website to contact customer service for details.

There are so many types of biological buffers, I believe I don’t need to say more, people know it whether they understand or don’t. Therefore, there are many uncertainties in our choice. I don't know which is what you need and which is suitable. In response to this problem, Desheng, as a manufacturer of biological buffers, teaches you how to choose.   1. Buffer range Each buffer has the highest buffering capacity in the pH range. This capacity is usually determined by the pKa of the buffer. You must choose a buffer with a pKa value close to the middle value of the desired range (usually, it is recommended to use a buffer with a pKa value at least within one pH unit of the target pH value). 2. pH changes during the experiment It is important to consider whether the pH may increase or decrease during the experiment. If you expect the pH to increase, you must choose a buffer with a pKa slightly higher than the optimal value at the beginning of the experiment. The opposite is also true: if you expect a decrease in pH, choose a buffer with a lower pKa.   3. Buffer concentration The concentration of the buffer must be adjusted to have sufficient capacity for the experimental system. In short, if the concentration of the buffer is too low, it will not be able to stabilize the pH of the solution. Conversely, if the concentration is too high, the buffer is likely to affect the experiment. Generally, it is recommended to use a concentration higher than 25 mM. For systems that do not actively exchange hydrogen protons, the recommended concentration is usually 25-100 mM. For systems where proton exchange may occur, it is recommended to use a buffer concentration 20 times higher than the molar concentration of exchanged protons. When preparing the buffer solution, remember to adjust the concentration of the solution to the intended use concentration. 4. Temperature changes The pH of the buffer solution must be set according to the temperature at which the experiment will be performed. Temperature directly affects the pKa of the buffer, which in turn affects the pH value and buffering capacity of the system. This situation may be very critical in biological systems. In biological systems, accurate hydrogen ion concentration is required to make the reaction system run at maximum efficiency. The pKa of some buffers (such as PIPES) is less sensitive to temperature changes, but other options (such as TRIS, TAPS, TES, or Tricine) are more affected by these changes.

Fingerprints play an important role in forensic identification and personal identification. At the same time, they are widely used in security inspection, access control, personal authentication and other fields in daily life. Although there are many ways to show fingerprints, a simple, fast and easy-to-operate method is still needed to show fingerprints. In this paper, based on the selective spatial control of the electrochemiluminescence behavior of luminol on the electrode surface, a potential fingerprint inverse imaging technology is constructed. The effects of the type of co-reactant, applied potential and luminophore concentration on the effect of fingerprint display were investigated. The imaging method has the advantages of simplicity, speed, and no need to process the substrate or sample, and provides a new method and new idea for fingerprint visualization and electrochemical imaging technology.   In the past ten years, chemists have continuously applied various new chemical analysis techniques to fingerprint display technology. Using mass spectrometry to form chemical imaging of latent fingerprints, although the imaging technology of image separation, infrared/Raman imaging, local current imaging and immunolabel imaging, etc., the fingerprint detection has been extended from a single morphological analysis to a lower finger resolution, but Its ability to recognize foreign substances is strong, and it can detect trace amounts of drugs of abuse contaminated on fingerprints, which has very important detection value and safety significance.   At present, although there are many kinds of fingerprint visualization methods, there is still a need for a simple, fast, and easy-to-operate method for fingerprint visualization. Luminol, also known as "luminol", can react with the iron ions in the heme and hematoporphyrin ring under alkaline and oxidizing conditions to emit a light blue light. Although luminol has a specific luminescence reaction with blood, the current formula of luminol can not overcome the technical problems of foaming, line blur, weak luminous intensity and short duration, so it is still used in practice for handprint visualization. There are certain limitations. In addition to chemiluminescence, luminol is also an important electrochemiluminescence reagent. Electrochemiluminescence is the product of the combination of electrochemical reaction and chemiluminescence. It is realized by converting electrical energy into radiant energy. It has the advantages of high sensitivity, controllable reaction, controllable time and space, and no background interference. In this study, the presence of potential fingerprints will cause the ECL activity of luminol on the electrode surface to be different. Using luminol as the ECL reaction system, by selectively controlling the generation of ECL on the electrode surface, the fingerprints carried on the electrode can be detected. Perform manifestation. It can clearly show the overall morphology and secondary structure of fingerprints, and can be used to show fingerprints on stainless steel objects. It should be noted that the concentration of luminol has a very large impact on the image imaging of ECL, and the intensity of ECL directly depends on the concentration of the luminophore. The research surface shows that when the concentration of luminol is lower than 5mmol/L, the ECL is too weak to effectively show fingerprint lines. When the concentration of luminol reaches 5mmol/L, the fingerprint morphology begins to appear, but when the concentration is higher, the ECL is too strong and it is not conducive to the appearance of fingerprints. Therefore, the most suitable luminol concentration is 5mmol/L.

There are many types of blood anticoagulants, and two of them are used more frequently. They are "heparin" and "EDTA". These two products each have a variety of models and specifications. Although the corresponding functions are not very different, there are also certain differences, and we will understand the differences between them together below.   The anticoagulation tube is to avoid blood clotting, and blood clotting is mainly formed by three reasons: 1. The formation of prothrombin activator; 2. The prothrombin activator turns prothrombin into active thrombin with the participation of calcium ions; 3. Soluble fibrinogen is transformed into insoluble fibrin under the action of thrombin. Fibrin is shaped like filaments, criss-crossed, and collects a large number of blood cells to form a jelly-like blood clot. This is how coagulation occurs. Anticoagulant mechanism of heparin: The heparin anticoagulation tube has a green cap, and heparin is added to the blood collection tube. Heparin has the physical and chemical properties of strong negative charge, which can interfere with many links of the blood coagulation process, and has anticoagulant effects both in vivo and in vitro. Its mechanism of action is relatively complicated, mainly through the combination with antithrombin III (AT-III), which enhances the latter's inhibitory effect on activated coagulation factors IIa, IXa, XIa, XIa and XIa. The consequences involve preventing platelet aggregation and destruction, preventing the formation of thrombin-activating enzyme; preventing prothrombin from turning into thrombin; inhibiting thrombin, thereby preventing fibrinogen from turning into fibrin. Neutralize tissue thromboplastin (factor III). Inhibit the aggregation and release of platelets. It is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, not suitable for blood coagulation test. Excessive heparin can cause the accumulation of white blood cells and cannot be used for white blood cell counting. It is not suitable for white blood cell classification because it can stain the blood slice with a light blue background.   The anticoagulant mechanism of EDTA: EDTA anticoagulation tube purple cap, ethylenediaminetetraacetic acid (EDTA, molecular weight 292) and its salt are an amino polycarboxylic acid, which can effectively chelate calcium ions in blood samples, chelate calcium or react calcium Removal will block and terminate the endogenous or exogenous coagulation process, thereby preventing the blood specimen from clotting. Suitable for general hematology tests, not suitable for coagulation tests and platelet function tests, and not suitable for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase , Suitable for PCR test. Therefore, the anticoagulant reactions of these two anticoagulants are different. For example, EDTA is a calcium chelator that reversibly binds to the Ca ions in the plasma to prevent blood clotting, while heparin acts with a kind of thrombin to prevent the coagulation pathway. Hubei Xindesheng Materials Co., Ltd. has been specialized in the production and research of blood collection tube additives for decades. The company's heparin and EDTA are the company's core products, and it has a professional R&D team management. In addition to blood collection tube additives, our company also produces other reagent products, such as: biological buffers, chemiluminescence reagents, chromogen substrates, enzyme preparations, carbomers, virus preservation solutions and other products. If you are interested in understanding our products, you can enter our official website and contact customer service for details. Hubei Xindesheng welcomes your visit.

In the field of in vitro diagnostics, both the chemiluminescence immunoassay CLIA and the immunofluorescence analysis IFA are commonly used detection methods, and ultimately both are detected by a photometer, but the principles of the two are essentially different. Compared with radioimmunoassay, fluorescence immunoassay, and enzyme-linked immunoassay, chemiluminescence immunoassay has more advantages. It has the characteristics of high sensitivity, strong specificity, wide linear range, simple operation, and does not require very expensive equipment. Moreover, it is radiation-free, the marker has a long validity period and can be fully automated.   Chemiluminescence reagent acridinium ester The difference between chemiluminescence immunity and fluorescence immunity: Although both are luminescence reactions, the most intuitive difference is that chemiluminescence is the reagent's own luminescence, while fluorescence is irradiated with a light source (usually ultraviolet) and then emits light. The light-emitting principle of the two is different, so the detection results will also be different.   Chemiluminescence is the use of energy generated by a chemical reaction to induce energy level transitions to emit light, such as luminol detecting bloodstains; fluorescence is a photoluminescence phenomenon, and a light source must be provided to excite molecules to produce energy level transitions, and then emit light. Chemiluminescence is less interference than fluorescence immunity: When these two methods are used for immunoassay, the difference is obvious. Chemiluminescence does not require an external light source, and the background interference is small; while fluorescence requires an external light source. When detecting in the direction of the vertical light source, the protein, amino acid and other molecules in the biological sample will also be detected. Background fluorescence is generated, and the background is slightly higher. It is necessary to select appropriate fluorescent reagents and sample processing methods to reduce the influence of non-specific adsorption of proteins. In the direct method, the antibody of each antigen must be fluorescently labeled, and the fluorescently labeled may affect the antibody titer or even become invalid. The indirect method only needs to make the corresponding antibody to the corresponding antigen, and then use the labeled secondary antibody to bind to it. It does not need to be fluorescently labeled for each antibody, and it has little effect on the titer of the primary antibody. The chemiluminescence interference is very small and the specificity is very high. The use of the whole method is restricted by the non-specificity of the chemical analysis itself. The development of magnetic bead materials has made the development of chemiluminescence technology more and more mature. In the development of in vitro diagnostic reagents, Desheng has achieved success in both enzyme-linked immunoassay reagents and chemiluminescence reagents. Among them, six acridinium esters, luminol and isoluminol are all necessary reagents in chemiluminescence immunoassays. .

Heparin lithium heparin sodium is a common heparin blood anticoagulant additive for heparin tubes. This year, customers who purchased lithium heparin have reported some questions to our company. For example, the doctor was using heparin tube to manipulate blood for electrolyte analysis and found that blood potassium and blood sodium were higher than the normal reference value. Why? After receiving the customer's feedback, we first performed repeated tests on the same batch of lithium heparin produced by our company, and determined that the various indicators are normal. We plan to explore the answer from two aspects. One is to find the conclusions of experts on the Internet, and to explore the answer from the doctor's professional point of view.     10g each bottle of lithium heparin packaging According to the examination of lithium heparin medical and clinical literature, the problem was found. By comparing the results of electrolyte measurement with lithium heparin anticoagulated plasma and serum without heparin substances, the mapping of lithium heparin to electrolyte measurement is analyzed. The method used is to draw 2 specimens of the same sample with different additives at the same time, a total of 40 specimens for comparison. The results of the experiment show that the anticoagulant effect of lithium heparin has a significant effect on the determination of blood potassium, and there is no statistically significant trend in the difference in the determination of blood sodium and blood chlorine. It is recommended that the electrolyte determination is better to absorb non-anticoagulated specimens. Analyzing the results of the study, Lunan found that compared with the serum electrolyte without heparin, heparin has no effect on the determination of blood sodium and blood chloride, but has an effect on blood potassium. Lithium heparin anticoagulant has a significant interference effect on potassium levels in electrolyte determination. From the analysis of the reason, heparin substance and potassium produce potassium heparin, which interferes with the determination of potassium by the ion-selective electrode, resulting in lower potassium ion content in the blood. At the same time, for serum samples, due to the destruction of platelets during the process of blood aggregation, potassium ions in the platelets (the concentration of which is much greater than that of plasma potassium) are released from human blood, making the serum concentration higher than that of plasma potassium ions, and the degree of increase is related to the number of platelets. In a positive relationship. In the practice of evacuation of serum, the true level of potassium in the body cannot be accurately reflected due to the fragmentation of platelets. Plasma potassium is used to replace the determination of serum potassium. According to common clinical experience, although there is interference from platelet destruction, serum potassium is still in view of the determination of plasma potassium, because blood serum potassium has no heparin influence, and the old domestic biochemical analysis and analysis widely accept serum as a specimen. From the perspective of professional doctors' clinical practice experience, this conclusion is also created. Heparin lithium heparin sodium is the heparin product represented by the main reaction reagent. After blood sampling, the anticoagulant reagent has a significant effect. Complete blood count and blood cell examination are often designed. Desheng Biochemical is deeply engaged in the field of blood collection tube preparations, and its products cover serum separation gel, heparin sodium, lithium heparin, dipotassium EDTA, tripotassium EDTA, coagulant, siliconizing agent, sodium citrate, potassium oxalate. 22 self-developed patented technology blessings. Ensure that the products that customers get are better than the average products of the peers, and enjoy high-quality after-sales technical services.

Heparin is named after it was found earlier in the liver. As a natural anticoagulant substance, heparin exists in many organs of mammals, among which the content of lung and intestinal mucosa is relatively high. Heparin is a mucopolysaccharide sulfate composed of glucosamine, L-iduronic acid, N-acetylglucosamine and D-glucuronic acid alternately, with an average molecular weight of 15KD and strong acidity. At present, after years of development, heparin has developed low molecular weight heparin. Both unfractionated heparin and low molecular weight heparin are commonly used non-oral anticoagulant drugs in clinical practice, and are mainly used to prevent and treat thrombosis or embolic diseases. And in clinical practice, there are many people who are vague about unfractionated heparin and low molecular weight heparin, and we will understand the difference between them in this article.   1. The difference in source Unfractionated heparin: Heparin, also known as unfractionated heparin, is a kind of dextran sulfate extracted from pig intestinal mucosa or bovine lung. Unfractionated heparin is a mixture with a molecular weight range of 3000-30000KD and an average molecular weight of about 15000KD. Low-molecular-weight heparin: Low-molecular-weight heparin is a general term for a type of heparin with low molecular weight prepared by depolymerization of unfractionated heparin. Its pharmacodynamics and pharmacokinetic properties are different from those of unfractionated heparin. The average molecular weight range is 3000-8000KD. 2. The difference in function Low-molecular-weight heparin: Low-molecular-weight heparin can be combined with antithrombin Ⅲ, resulting in structural changes of antithrombin Ⅲ, thereby accelerating the inhibitory effect on factor Xa, producing a strong anticoagulant effect, and helping to relieve and reduce coronary arteries Lumen obstruction improves myocardial ischemia. It is also difficult to eliminate in the body and has a long action time. It rarely affects platelet function, does not reduce its number, and has a strong effect of inactivating platelet surface coagulation factor Xa. Unfractionated heparin: As an anticoagulant, heparin is a polymer formed by alternately connecting two kinds of polysaccharides. It has anticoagulant effects both in vivo and in vitro. Clinically, it is mainly used for thromboembolic diseases, myocardial infarction, cardiovascular surgery, cardiac catheterization, extracorporeal circulation, hemodialysis, etc. With the advancement of pharmacology and clinical medicine, the application of heparin continues to expand. 3. The difference in application Unfractionated heparin: Thrombosis or embolic disease; Disseminated intravascular coagulation caused by various reasons; It is also used for hemodialysis, extracorporeal circulation, catheterization, microvascular surgery and other operations and the anticoagulation treatment of certain blood samples or instruments. Low molecular weight heparin: Prevent venous thromboembolic diseases, especially deep vein thrombosis after orthopedic or general surgery; Treatment of deep vein thrombosis that has formed; For the treatment of non-ST-segment elevation myocardial infarction in the acute phase of unstable angina; Prevents the formation of blood clots in the dialyzer during hemodialysis. Although low molecular weight heparin is a derivative of unfractionated heparin, it cannot replace unfractionated heparin in some aspects. And in terms of price, the price of low-molecular heparin is much higher than that of unfractionated heparin, so unfractionated heparin has more priority in other fields, such as blood collection tube additives. Our company is a manufacturer specializing in the production of heparin sodium/lithium, an additive for blood collection tubes. Hubei Xindesheng Material Co., Ltd. is a professional manufacturer of blood collection tube additives. The company has been established for decades. Among them, the heparin series is our company's core product, which is used in the development and production of professional teams. If you are interested in understanding heparin, you can enter our official website to contact customer service for consultation. Hubei Xindesheng welcomes your visit.

Acridine ester is a chemical substance that can be used as a chemiluminescent label. Acridine salts and related compounds have been widely proven to be very useful chemiluminescent labels. Its stability, labeling specificity and detection sensitivity surpass that of radioisotopes. . Under alkaline conditions, NHS is substituted as a leaving group, and the protein forms a stable amide bond with the acridine ester. However, acridinium ester is only a general term for acridinium salt. If subdivided, Desheng can provide 7 different acridinium esters (see the table below) for you to choose from, so what are these 7 acridinium esters? What are the similarities and differences? Let us continue to look down.   Magnetic particle chemiluminescence method using acridinium ester 1. Desheng acridine ester types: 1.1 Acridinium Ester No. 0-Acridinium Ester (AE-NHS) 【CAS】None 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】N/A 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.2 Acridinium Ester No. 1-Acridinium Ester (DMAE-NHS) 【English name】2',6'-dimethyl-4'-(N-succinimidyloxycarbonyl)phenyl-10-methyl-acridinium-9-carboxylate methosulfate [Chinese name] 9-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,6-dimethylphenoxy]carbonyl]-10-methyl Acridine Methyl Sulfate 【CAS】115853-74-2 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】594.13, C29H26N2O10S 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours.   1.3 Acridinium Ester No. 2-Acridinium Ester (Me-DMAE-NHS) 【English name】2',6'-Dimethylcarbonylphenyl 10-Methyl-9-acridinecarboxylate 4'-NHS Ester Triflate [Chinese name] 2',6'-Dimethylcarbonylphenyl 10-methyl-9-acridine carboxylate 4'-NHS ester trifluoromethanesulfonate 【CAS】None (115853-74-2, DMAE-NHS analogue) 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】632.56, C29H23F3N2O9S 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours.   1.4 Acridinium Ester No. 3-Acridinium Ester (NSP-DMAE-NHS) 【English name】2',6'-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'- NHS Ester [Chinese name] 9-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,6-dimethylphenoxy]carbonyl]-10-( 3- Sulfopropyl)-acridine inner salt 【CAS】194357-64-7 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】590.6, C30H26N2O9S 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours.   1.5 Acridine ester No. 4-acridine salt (NSP-SA) [English name] 3-[9-(((3-(carboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonateinner salt [Chinese name] Acridine salt (NSP-SA) 【CAS】211106-69-0 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】584.66, C28H28N2O8S2 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours.   1.6 Acridine ester No. 5-acridine salt (NSP-SA-NHS) [English name] 3-[9-(((3-(N-succinimidyloxycarboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonateinner salt [Chinese name] Acridine salt (NSP-SA-NHS) 【CAS】199293-83-9 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular Weight】681.73, C32H31N3O10S2 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours.   1.7 Acridine ester No. 6-acridinium hydrazide (NSP-SA-ADH) 【CAS】None 【Appearance】Yellow solid or powder 【Purity】≥98% 【Molecular weight】740.85, C34H40N6O9S2 【Storage conditions】-20℃, avoid light [Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. two. The structural difference of acridine esters Six kinds of acridine esters, of which Nos. 1 to 3 are acridine esters; Nos. 4 to 6 are acridine sulfonamide lactates; Nos. 1 to 4 are NHS active esters; No. 5 is acridine carboxylic acid with carboxyl group; 6 The number is acridine hydrazide, which contains free amino groups.   three. Hydrolysis resistance and stability Traditional acridinium ester No. 0 (AE-NHS) and No. 1 to 3 have been modified in their structure to increase steric hindrance and enhance hydrolysis resistance. No. 0 is only stable in acidic solutions. When the pH value is greater than 6.3, it is easy to hydrolyze, while No. 1 to 3 are not. For example, at room temperature, it is very good in PB buffer with pH 7.0. It is stable. After 16 days, the luminescence activity is only reduced by 3.6%. No. 4 to No. 6 is the introduction of sulfonamide groups, and its stability is higher than that of acridine esters (No. 0 to No. 3). The reason is that the C-N bond and C-O bond are more stable. The bond level is different, the C-N bond is larger than the C-O bond. Acridine amide compounds (No. 4～6) have stronger resistance to hydrolysis than acridine esters (No. 0～3). Things. No. 4 to No. 6 are stable in acidic solution (pH

Tris, Hepes, Mops and other buffers are almost all made up of buffer pairs composed of "weak acid and its conjugate base salt" or "weak base and its conjugate acid salt". You can add a certain amount of other buffers. A substance that slows down changes in PH. These two substances can be buffered toward acid or base at the same time because of the reaction. For example, a mixed solution of acetic acid and sodium acetate is a buffer solution. If hydrochloric acid is added, the pH will not drop too fast, because hydrochloric acid will react with sodium acetate to produce acetic acid. On the contrary, if sodium hydroxide is added, the pH will not increase too fast, because sodium hydroxide will react with acetic acid to form sodium acetate. In many chemical reactions, buffer solutions are used to keep the pH of the solution constant. Buffer solutions are of great significance to the production and evolution of life, because most organisms can only grow within a certain pH range. There are many types of biological buffers, and here will introduce several commonly used biological buffers. 1. Tris buffer: Chinese name Tris(hydroxymethyl)aminomethane, CAS number 77-86-1, white crystalline powder, odorless and tasteless, good water solubility. It is a widely used buffer with a pKa of 8.1 at 25°C and a pH buffer range of 7.0-9.2. It is mostly used in chemical experiments, new coatings, cosmetics and other fields. 2. Hepes buffer: Chinese name 4-hydroxyethylpiperazine ethanesulfonic acid, CAS number 7365-45-9, white crystalline powder in appearance. It belongs to the "Good" buffer and is also one of the most suitable buffers for biological research. It is a zwitterionic organic buffer with a pH buffer range of 6.8-8.2. It is mostly used in cell culture, electrophoresis experiments, cosmetics and other fields. 3. Mops buffer: Chinese name 3-morpholine propane sulfonic acid, CAS number 1132-61-2, white powder in appearance. It is a zwitterionic buffer commonly used in biochemistry and molecular biology. It belongs to the "Good" buffer like Hepes. The pH buffer range of Mops is 6.5-7.9, and the pKa is 7.2 at 25°C. It is mostly used as physiologically related buffers, DNA/RNA extraction kits, PCR diagnostic kits, etc. Nowadays, these buffers are often used in biochemical experiments, so the market demand for such buffers is very large. Therefore, buffer merchants on the market are mixed, making the procurement work very difficult. Recommend a manufacturer here: Hubei Xindesheng Material Co., Ltd. Desheng company has been producing chemical raw materials for decades. Desheng company has a professional team responsible for the development and production of biological buffers, and has imported equipment and raw materials for production and processing. The products produced by Desheng Company are of high quality, fast production efficiency, and sufficient stock in the warehouse. If you are interested in Desheng's products, you can enter the company's official website to contact customer service for details.