China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Introduction: Carbomer 940, CAS 9003-01-4, also known as carbomer 940, is a kind of high molecular polymer cross-linked by acrylic acid and acryloyl sucrose or acryloyl pentaerythritol. It is a white loose powder with characteristic slight odor and strong hygroscopicity. Its average water content is 8%. Carbomer resin exists in water in the form of acid. It is easy to swell in water and polar organic solvents (such as ethanol, glycerin, etc.) Characteristics of colloidal solution. Because of 56% - 68% carboxylic acid group in the molecule, these resins are weakly acidic. Although they are weaker than acetic acid, they can easily react with inorganic bases and organic bases to form salts.   Because of its swelling and weak acidity, it is a very important rheologic regulator. The neutralized Kabo resin is an excellent gel matrix with thickening, suspension and other important properties. It has high transparency, high viscosity, strong suspension ability, very short rheology and thixotropy, low ionic shear resistance, simple process and good stability. It is widely used in shampoo, shower gel and bath. Cream, cream and other cream products are especially suitable for transparent products. The most important point is that carbomer 940 is mild and not irritant to skin. Carbomer 940 powder matters needing attention: 1. PH value: the best pH value range of Carbopol 940 is 4-10, higher or lower than this range will lead to changes in the viscosity of the system.   2. The components in the formula that are not easy to be compatible: protein, PVP resin, polyethylene glycol (PEG), polyethoxylated surfactant will interact with the non neutralized Carbopol 940, and it is necessary to partially neutralize Carbopol 940 before adding.   3. The presence of electrolyte will reduce the thickening efficiency of Carbopol resin: Carbopol 940 is sensitive to salt and cation, when the concentration of soluble ion in the component is greater than 0.1%, the dosage of the component should be reduced appropriately.   Deionized water can be used as the matrix of the main material, and salt can be added after neutralization to master the influence of salt on the system. High valence ions (CA, Mg, Fe, AI) will cause serious damage to the system and should be removed. The Fe and Cu converted in the production process will reduce the viscosity of the system and lead to the instability of the system. Stainless steel or non-metallic equipment can be used to prepare products to avoid such effects. In addition, adding EDTA to chelate metal ions can also achieve good results. In addition to the above methods, the appropriate model of Carbopol 940 (such as Carbopol 940l342ge resin) can also be selected, which has low sensitivity to soluble salts.   4, insoluble matter: when there are insoluble components, carbo 940 system is difficult to present clear and transparent gel, and solubilization technology can reduce or eliminate this effect.   5. High shear agitation and pump delivery: carbo 940 is thickened by forming a gel skeleton. After Carbopol neutralization, high shear stirring, long-term stirring, grinding or pump chipping will damage its skeleton and cause viscosity loss, so pipeline homogenizer should be used. Use low shear pumping, such as reciprocating diaphragm pump or gear pump.   6. Long term UV irradiation can reduce the viscosity of carbomer resin.   7. Packing: it can be sealed with moisture-proof and airtight plastic bags, and then packed in cardboard boxes. Carbomer 940 has strong hygroscopicity. During storage, attention must be paid to sealing, and care must be taken to avoid moisture absorption and caking in the exposed air. If there is slight caking, it can become loose powder by gently patting and pressing, which will not affect the thickening effect. After use, it is better to put it in a sealed container with desiccant.   8. Transportation: carbomer is polyelectrolyte, sometimes static when rubbing or extruding resin particles. In case of splashing, remove the dry powder with a broom first. Do not rinse with water. Otherwise, it will form very slippery gel on the ground or equipment.   Hubei xindesheng company specializes in providing carbomer 940, 980 products and after-sales technical support. If you have any questions, please call for details.

1. As an inducer and self-oxidant Luminol is a light inducer and self-oxidant. It can be used as a free radical detection reagent as well as a photosensitizer. In PCL analysis, auto-oxidation is inhibited by a single or a group of antioxidant compounds in the nanomolar range. The antioxidant potential of the sample can be measured by studying the lag phase at different concentrations.   2. Detection of blood luminescence reagent The blood absorbs ultraviolet rays and turns black. After chemical treatment, the blood will also fluoresce. Luminol is commonly used to produce this chemiluminescence, which is a reagent that reacts with catalase in hemoglobin in the blood.   3. Time interval used to identify skeletal remains As the femoral PMI increases, chemiluminescence decreases. In femurs with a PMI of 1 month to 3 years, strong chemiluminescence is observed after a few seconds. Patients with a PMI of 10-15 years have clear chemiluminescence, and 80% of the samples are visible to the naked eye; femurs with a PMI of 25-35 years have weak chemiluminescence in 33% of the samples. Femurs with a PMI of 50-60 years have only a weak response observed in a single femur. No chemiluminescence was observed in femurs whose PMI was over 80 years old.   4. Detect the influence of serum prostate specific antigen The prostate-specific antigen in serum can be detected by luminol chemiluminescence enzyme immunoassay, which has high sensitivity, simple and quick operation, and strong specificity. When tPSA is greater than 10ng/ml, prostate cancer is highly suspected. When tPSA is between 4-10ng/ml, it is called gray value, and the diagnosis of prostate cancer should be combined with the ratio of fPSA/tPSA.   5. Bioluminescence imaging for inflammation Luminol can achieve highly sensitive bioluminescence imaging of inflammation through the luminescence reaction with myeloperoxidase MPO produced in the inflammatory area, which is important for the diagnosis of neurodegenerative diseases, atherosclerosis, cancer and other diseases significance.

Enzymes are the soul that catalyzes various reactions in nature. Many originally difficult chemical reactions can be easily handled by enzymes that are invisible to the naked eye, playing the role of superheroes in daily life. The greater the ability, the greater the responsibility; then what kind of enzyme preparation can be a superhero? The time to test them has arrived.   The first level: higher specific vitality The ratio of enzyme activity to enzyme weight is called enzyme specific activity. This is a bit like a weightlifting competition. Two players with the same weight, whoever can lift a heavier weight wins. Why should we examine the specific vitality? Because the higher the specific activity, the less enzymes are needed to accomplish the same job. Enzyme itself is also a kind of protein, which can be a nutrient for microorganisms. The more enzymes also represent the more nutrients, the greater the possibility of breeding microorganisms. Therefore, the higher the specific activity of the enzyme, the lower the possibility of microbial contamination, which is vital to the stability of the catalytic reaction system. The second pass: good thermal stability Like food, enzyme preparations have a shelf life. This is because the specific activity of the enzyme preparation will slowly decrease over time, and when it drops to a certain level, the enzyme cannot continue to perform its expected function. In a high temperature environment, the enzyme activity will decrease faster. Thermal stability is an important reference for judging enzymes. Enzyme preparations with good thermal stability can withstand the toss of environmental temperature changes and have more obvious advantages in terms of validity and transportation.   The third pass: a wide range of pH stability   pH is a parameter describing the degree of acidity and alkalinity of the aqueous solution. Enzymes have different catalytic activities in different pH environments; enzyme preparations can maintain stable activity within a certain pH range, and over-acid or over-alkali will reduce the stability of the enzyme. If an enzyme can maintain high activity in a wide pH range, it means that the enzyme has good compatibility with catalytic reaction systems of different pH and is the first choice for consideration. Fourth hurdle: specific substrate specificity One must be dedicated to being a human being, and dedicated to doing enzymes. If an enzyme preparation is not selective to the substrate, the products of the catalytic reaction will be diverse and uncontrolled. A specific enzyme preparation will only convert substances with the same structure into products. Even if it is surrounded by other substances, it will only "love" the only substrate, and there will be no stories that shouldn't happen.   The fifth level: strong anti-interference ability In the catalytic reaction system, in addition to enzymes, other substances will be added as needed; the raw materials participating in the reaction will also carry certain impurities. The appearance of these substances will affect the activity of enzyme preparations. Even with the appearance of interfering substances, excellent enzyme preparations will continue to show good vitality and continue to play a core role in inducing reactions.   Enzyme preparations that can pass these five tests have the basic qualities to become a superhero. However, the distance allows these superheroes to exert their powerful catalytic ability, and there is still a long way to go. In the future, Desheng Technology will continue to introduce other knowledge about enzymes for you.

Biological buffers are an important part of electrophoresis technology and are generally added to the solution to stabilize the pH when current is passed through the sample. In addition, the buffer can also provide the ions needed for electrophoretic migration.   The ideal buffer for electrophoresis depends on the isoelectric point of the sample being analyzed.   Although there are many pre-made buffers for electrophoresis on the market, it is recommended to use the biological buffers prepared by yourself to make your own solutions. Our company is a professional manufacturer of biological buffers. For this reason, we have created a list of biological buffers frequently used in electrophoresis, so that it is convenient to see which biological buffer is more suitable for you.   1. Tris buffer Used for: gel electrophoresis Useful pH range: 7.5-9.0 pKa (25°C): 7.8-8.2 Molecular weight: 121.14   2. MOPS buffer Used for: gel electrophoresis Useful pH range: 6.5-7.9 pKa (25°C): 7.0-7.4 Molecular weight: 209.3   3. Bicine buffer Used for: gel electrophoresis Useful pH range: 7.6-9.0 pKa (25°C): 8.1-8.5 Molecular weight: 163.2   4. CAPS buffer Used for: Capillary Electrophoresis Useful pH range: 9.7-11.1 pKa (25°C): 10.2-10.6 Molecular weight: 221.32   5. CAPSO buffer Used for: gel electrophoresis Useful pH range: 8.9-10.3 pKa (25°C): 9.4-9.8 Molecular weight: 237.32   It can be seen from the above that there are many biological buffers used in electrophoresis, and there are some differences between them, and they can be used in different electrophoresis. In the current market, there are many vendors of biological buffers, and product specifications and quality are uneven. Our company is a manufacturer specializing in the production of biological buffers. It has been established for more than ten years and has very rich R&D and production experience and product knowledge. It can provide customers with a large amount of technical support and after-sales guarantee.   Hubei Xindesheng Materials Co., Ltd. can also provide customized packaging and other services according to customer needs, and our products can be delivered to most areas at home and abroad. In addition, our company also produces blood collection tube additives, chemiluminescence reagents, chromogen substrates, carbomers and other products. If you are interested in our products, you can enter our official website to contact customer service for details.

3 - (cyclohexylamine) - 1-propanesulfonic acid (CAPS) is a kind of sulfamate, cas1135-40-6. Its molecular formula is c9h19no3s, and its molecular weight is 221.32. It is white crystalline powder. Amphoteric, the most widely used is in water-based polyisocyanate coatings and buffer used in enzyme chemistry and HPLC separation of basic drugs. Desheng caps products   In biological buffer: buffer for enzyme chemistry and HPLC separation of basic drugs.   Product application: in biological experiments, it is an important pH stabilizing reagent, usually choose the appropriate weak acid and its conjugated base to get the appropriate pH value. Most of the biological reactions take place under neutral conditions, generally pH is between 6-8, and the effective buffer range of buffer is also between 6-8. In addition, the acid-base form of buffer should not chelate with some metal ions, such as in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit.   In the new coatings, 3 - (cyclohexylamino) - 1-propane sulfonic acid (CAPS) reacts with aliphatic polyisocyanate (the former is zwitterionic sulfamate) under mild conditions and in the presence of tertiary amine neutralizer, and the sulfonylurea derivative is an excellent emulsifier. Without considering the salt group, CAPS modified polyisocyanate has good storage stability and is not cloudy. Even if it contains less sulfonate base group, it can also get very good emulsion in water. A series of ionized polyisocyanates can be obtained, which can be used in various environment-friendly high-quality waterborne two-component polyurethane coatings. These coatings can be compared with general solvent based coatings in terms of dryness, curing and chemical resistance.   In addition to the above applications, it is also a raw material for manufacturing welding materials, air conditioning equipment and lithium metal. It is also used for making pyrotechnics, analytical reagent, heat exchange carrier, pharmaceutical industry, air conditioning, pyrotechnics, dry battery and lithium metal, as well as flux and desiccant. From these applications, I can see that 3 - (cyclohexylamine) - 1-propanesulfonic acid plays an extraordinary role in our daily life. We Desheng company is to find these advantages, it has been 15 years of R & D and production, hoping to meet the needs of customers.

Desheng is a material technology company engaged in R & D, production and sales. We will make a series of comparisons for each series of products, so as to help customers better distinguish the differences between them and better find the products they need. Let's briefly describe the differences and similarities between good's buffer pipe and HEPES.   PIPES The pH buffer range of pipes is 6.1-7.5, insoluble in water and soluble in NaOH solution. Different from buffers containing bis (2-hydroxyethyl) amino groups (such as bis Tris, bicine), pipes can not form stable complexes with most metal ions, so it is suitable for buffers containing metal ions. According to previous studies, PIPES can be used to purify tubulin by using cellulose phosphate chromatography, and to purify recombinant GTP binding protein ARF1 and ARF2 by gel filtration as a buffer to crystallize ketoenzyme from E. coli. In addition, due to the formation of free radicals, pipe is not suitable for redox system. In cation exchange chromatography, low concentration of pipes buffer should be used because of its relatively high ionic strength and concentration dependent pKa value.   HEPES The pH buffer range of HEPES is 6.8-8.2, which is soluble in water and does not form stable complexes with metal ions. In most cases, it does not interfere with biochemical processes. HEPES is commonly used in cell culture media of various types of organisms; in protein research, it is commonly used as component and eluent of binding buffer in cation exchange chromatography; in DNA research, it is used as calcium phosphate and DN A, the buffer solution of precipitate formation system, AFM and electroporation experiment. In addition, HEPES interferes with the reaction between DNA and restriction enzyme and is not suitable for Lowry's method.   Desheng good's buffer   In conclusion, both pipes and HepS belong to good's buffer, and they can not form stable complexes with metal ions, so they are suitable for solution containing metal ions. However, there are some differences between them. In terms of solubility, pipe is insoluble in water, while HEPES has good water solubility. In terms of buffer range, pipe is acidic to neutral, and HEPES is neutral to alkaline. This is mainly due to the structural differences between them. Pipe has two sulfonic groups, and HEPES contains one sulfonic group and hydroxyl group. In addition, pipes and HEPES have some limitations in the application of some systems. Therefore, when we choose the above buffers, we need to consider the suitability of the experimental system and the difference of their properties.   When you purchase good's products, it's not the buffer you need. Desheng will also give you an accurate positioning.

I suspect that many display skills to the full play of the classic criminal investigation forensic TV series, such as forensic pioneer, legal life, and many other Hong Kong dramas. People usually suspect that they will clean up their blood after the crime and try to get more opportunities to get rid of their crimes. At this time, Lumino is the time to show his talents. The iron in blood immediately catalyzes the chemiluminescence reaction of luminol and makes it produce blue light. Because of its high efficiency and convenient use, luminol has been widely used in some criminal investigation cases.   Luminol, also known as luminol. In forensic medicine, the luminol reaction can identify blood stains that have been scrubbed for a long time. In biology, luminol is used to detect the presence of copper, iron and cyanide in cells. At room temperature is a blue crystal or pale yellow powder, is a relatively stable synthetic organic compounds. The chemical formula is c8h7n3o2. At the same time, luminol is a kind of strong acid, which can stimulate eyes, skin and respiratory tract. Hemoglobin contains iron, which can catalyze the decomposition of hydrogen peroxide, turning hydrogen peroxide into water and monooxygen, which then oxidizes luminol to make it glow. Therefore, luminol is widely used in criminal investigation, bioengineering, chemical tracing and other fields.   Although the dosage of luminol is relatively small and it is not difficult to detect, we should pay attention to some problems when using luminol. Here are some matters needing attention when using luminol for formal investigation.   1. Luminol fluoresces in the presence of iron, ferroalloys, horseradish or some bleaching agents. So if the crime scene is completely bleached, luminol's fluorescence will strongly mask the presence of any blood stains.   2. Luminol may interfere with other tests, but luminol does not interfere with DNA extraction.   3. The use of luminol needs to be in a dark environment, which makes it easier to make a more accurate judgment with the naked eye.   4. Luminol luminescent time is limited, we should seize the time to take photos and record.   5. The illumination lasts about 30 seconds, which can be seen from long exposure photos. The surrounding environment should not be too bright.   Currently, the blood test reagent luminol produced and developed by Desheng is very mature, with the advantages of high sensitivity and high luminous efficiency. It plays an important role in criminal investigation cases, and also meets the needs of many criminal investigation enthusiasts to carry out chemiluminescence experiments by themselves. Luminol can not only be used as blood test in criminal investigation, but also can be used for chemiluminescence immunoassay.

Daos, Chinese name n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3, 5-dimethoxyaniline sodium salt, the finished product is white or light blue powder, CAS number is 83777-30-4, molecular formula is c13h20nnao6s, molecular weight is 341.36, Daos is a new kind of Trinder's reagent, with high water solubility, stable reaction, wide pH range, as an excellent color reagent is widely used in diagnostic detection and biochemical experiments.   The detection principle is: in the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent reacts with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolylsulfonylhydrazone (MBTH) to form a very stable purple or blue dye, and then the substrate concentration is determined by spectrophotometry. Daos chromogenic reagent   1、 The advantages of Daos in blood glucose measurement experiment are as follows The determination of glucose in blood is an important test item in clinical medicine. At present, the commonly used detection method is glucose oxidase coupled with peroxidase method. This method uses God to catalyze the oxidation of glucose to gluconic acid and produce H2O2. H2O2 oxidizes colorless reduced chromogen under the catalysis of pod to produce colored oxidized pigment, and then calculates the content of glucose in the sample according to the color of oxidized chromogen.   The initial reaction of this method is specific, but the indicator reaction is nonspecific. The detection performance of this method changes with the different reduction chromogens in the indicator reaction. The common reduction chromogens are linaniline (which is rarely used because it is suspected to be carcinogenic), and the improved Trinder's reagent Daos is coupled with 4-aminoantipyrine (AAP) as the reducing chromogen The maximum absorption wavelength of the oxidized pigment formed by the oxidation and condensation of Daos and AAP is 592nm. Under this wavelength, bilirubin and other substances in blood do not interfere with the absorption, which can reduce the absorption interference of bilirubin and other interfering substances on blood glucose detection.   Therefore, this method does not need to separate blood into serum, which is simpler and more accurate than the method widely used in clinical enzymatic analysis with phenol as the reduced chromogen. The linear range of glucose is 1 ~ 20mmol / L, and the recovery is 96.3% ~ 97.7%.   2、 Instruments and reagents: UV VIS spectrophotometer, glucose oxidase, peroxidase, Daos, AAP, anhydrous glucose, citric acid and trisodium citrate dihydrate, glycerin, bovine serum albumin, blood glucose detection kit, God and pod were prepared with pH = 6 buffer solution, and other reagents were prepared with distilled water.   3、 Methods: the experiment was carried out In 1cm cuvette, add 0.5ml (25.6u) of God, 0.5ml (11.5u) of pod, 0.1ml (1.7mmol / L) of Daos, 0.1ml (2.9mmol / L) of AAP and 0.8ml of distilled water in turn, then add 40ul (8.3mmol / L) of glucose solution, stir well and use distilled water as reference to determine the absorption spectrum.   Desheng biochemical focuses on the development and production of in vitro diagnostic reagent raw materials, mainly including blood collection additive, chemiluminescent reagent, color reagent, enzyme substrate, antigen antibody and other products. The products are widely sold in domestic and foreign markets. Desheng's goal is not to be big, but to be a strong person in the precise field of the industry and win the market trust with its own professional.

Tris, CAS: 77-86-1. It is a white crystal or powder, soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, corrosive to copper and aluminum. Tris has high buffer capacity, high solubility in water and is inert to many enzyme reactions, which makes Tris a very satisfactory buffer for many biochemical purposes. It is used to stabilize the pH of the reaction system and has a strong buffer capacity between pH 7.5 and 9.0.   Desheng Tris packaging   Tris in glyphic acid buffer system was used to stabilize pH value in the electrophoretic buffer solution. The Tris-HCl buffer system was used to stabilize pH value in the gel. It was widely used as a solvent for nucleic acids and proteins. The low ionic strength of Tris buffer could also be applied to the formation of intermediate fibers of nematodes. EDTA buffer was added into Tris hydrochloric buffer to make TE buffer, which could be used for the stabilization and storage of DNA. The "Tae buffer" can be obtained by replacing the acid solution with acetic acid, and tbe buffer can be obtained by replacing it with boric acid. These two buffers are often used in nucleic acid electrophoresis experiments.   Tris is used in both TAE and tbe buffer (for nucleic acid dissolution) in biochemical experiments. It contains amino groups and can react with aldehydes. Tris is a weak base and its PKA is 8.1 at 25 ℃. The effective buffer range of Tris buffer is between pH 7.0 and 9.2.   The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain the buffer solution with this pH value. At the same time, we should pay attention to the effect of temperature on pKa of Tris. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in such a solution to improve its solubility. People often add EDTA into Tris hydrochloric acid buffer to make "Te buffer", which is used for DNA stabilization and storage. If the acid solution of adjusting pH value is replaced by acetic acid, the "Tae buffer" (Tris / acetate / EDTA) is obtained, and the "tbe buffer" (Tris / borate / EDTA) is obtained by replacing it with boric acid. These two buffers are usually used in nucleic acid electrophoresis experiments.   TAE and tbe made from Tris are commonly used in DNA electrophoresis. Te (ph8.0) is mainly used to dissolve DNA. (TE is Tris plus EDTA.) 1mtris-hcl6.8 and 1.5mtris-hcl8.8 are commonly used in SDS-PAGE. Tris buffer has been used more and more in biochemical research, with the trend of more than phosphate buffer. Tris buffer has been used in SDS- polyacrylamide gel electrophoresis, and phosphate is rarely used. The common effective pH range of Tris buffer is in the "neutral" range.   In Tris medium, a certain microorganism can produce certain metabolites after growing in the medium, which can react with special chemicals in the medium and produce obvious characteristic changes. According to this characteristic change, this kind of microorganism can be distinguished from other microorganisms   In Tris HC, an amino group in it is a coordination group, which may replace the ligand in the original complex, thus changing the complex and affecting the absorbance. If you want to know whether there will be such an effect, you need to check their coordination constants and compare them.   Desheng has 15 years of experience in R & D and sales of biological buffers, and has generally received high praise from customers. There is still a long way to go in the future, and we will make persistent efforts to forge ahead!

HEPES is 4-hydroxyethyl piperazine ethanesulfonic acid in Chinese, CAS number is 7365-45-9, pH buffer range is 6.8-8.2, pKa value is 7.5 at 25 ℃. HEPES Na is n - (2-hydroxyethyl) piperazine-n '- (2-ethanesulfonic acid) sodium salt, its CAS number is 75277-39-3. HEPES and HEPES Na are very important biological buffers, which are widely used in biopharmaceutical, medical diagnosis and other fields.   Packaging of biological buffer series products HEPES is a kind of amphoteric buffer, which is used in cell culture medium and protein research for various types of organisms. It can also be used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit. Because of its non-toxic effect on cells and the ability to control the constant pH range for a long time, HEPES is often used as cosmetic additive, active agent, peeling agent and promoter Through the role of the agent. Difference between HEPES and HEPES Na: HEPES Na, also known as organic HEPES base, is a conjugated acid-base relationship with HEPES. They are essentially the same, but the pH of the two substances after dissolution is different.   The preparation methods of HEPES were as follows About the preparation of HEPES buffer solution, according to the use of preparation, one is pure HEPES + NaOH, the other is HEPES + salt. Various preparation methods are summarized as follows:   1、 Preparation of 500 ml 1 m HEPES, pH = 7.0, stock solution Dissolve 119.15 g HEPES in 400ml distilled water, add 0.5 ~ 1m NaOH aqueous solution to adjust at least the required pH (the effective pH range of HEPES is 6.8 ~ 8.2), then fix the volume to 500ml with distilled water and store at 4 ℃.   2、 HEPES buffer formula with small amount of salt (500ml) HEPES 6.5g, NaCl 8.0g, na2hpo4.7h2o 0.198g, adjust the pH value with 0.5m NaOH solution, and finally fix the volume.   3、 Preparation of 2 × HEPES buffer salt solution Dissolve 1.6g NaCl, 0.074g KCl, 0.027g na2hpo4.2h2o, 0.2g dextran and 1g HEPES in 90ml distilled water, adjust to the required pH value with 0.5m NaOH, and then fix the volume to 100ml with distilled water.   The preparation method of HEPES Na was as follows HEPES Na can be synthesized by 4-hydroxyethyl piperazine and sodium vinyl sulfonate, or by high pressure synthesis of hydroxyethyl sulfonic acid, sodium hydroxide and 4-hydroxyethyl piperazine. At present, the most widely used preparation method that meets the requirements of biological buffers is the reaction of HEPES with NaOH to produce HEPES Na. The specific preparation steps are as follows:   Under the protection of nitrogen, HEPES with purity between 90-99% and NaOH solid or solution were reacted for 0.2-1 hour at 20-100 ℃. After the reaction, the obtained product was decolorized, filtered, concentrated, dehydrated, crystallized, washed and dried to obtain HEPES Na.   This method is simple and easy to operate, and the yield and purity of HEPES Na product are high, which can meet the purity requirements of biological buffer.   Desheng has 15 years of experience in developing and producing series products of biological buffers (Tris, HEPES, caps, mops, pipes). Desheng has deep research on blood vessel additives (heparin lithium, heparin sodium, dipotassium, Tripotassium), chromogenic reagents (toos, Maos, tops, Alps), chemiluminescence reagents (luminol, isoluminol, acridine ester). Desheng has checked the production and packaging of selected materials layer by layer, and insisted on improving the quality of products for customers Provide high quality raw materials for products.

Serum separation gel, a kind of raw material commonly used in hospital laboratory, is indispensable for many biochemical analysis. It is an inert polymer, insoluble in water, and has the characteristics of high temperature resistance, low temperature resistance, oxidation resistance and high stability. Some manufacturers such as Desheng also have the characteristics of anti irradiation. Packing and delivery of serum separation gel The main purpose of serum separation gel is to form an isolation layer between blood cell components and serum, which can effectively prevent the material exchange between blood cells and serum, ensure the stability of serum components within a certain period of time, and make the test results closer to the biochemical level. The separation gel collecting vessel with coagulant can shorten the blood coagulation time, quickly get the serum and the processed blood The blood sample can withstand the shaking and bumping of long-distance transportation. The isolation layer of separation glue can adhere to the test tube tightly after centrifugation. The serum can be directly drawn from the automatic analyzer in the original state or stored in cold storage. The long-distance transportation does not affect the test results, and also avoids the influence of fibrinogen and hemolysis.   In addition, a series of processes such as blood sample injection into blood collection vessel, serum separation, analyzer direct serum collection, blood sample preservation and waste disposal are carried out in the same branch pipe, which can avoid the contamination of blood samples, prevent the infection of virus in blood, and ensure the biological safety of the test process.   With the improvement of people's health awareness, blood testing has become more common. Serum separation glue is favored by various medical institutions, and there are many manufacturers of separation glue. Due to the different requirements of medical institutions for separation glue, the components of separation glue produced by each manufacturer are different, no matter from the transparency, color, viscosity, specific gravity and other aspects of performance. High quality serum separation gel can shorten the time of serum separation, and the separation gel is between serum and blood cells, which can effectively protect the serum components, so as to realize the original tube on the machine, save the serum in the original tube for future reference, and reduce the possibility of error caused by re tube transfer.   However, the influence of inferior separation gel on the test items can not be ignored. The use of inferior separation gel can make the triglyceride (TG) in the test results increase abnormally. Therefore, the comparative test must be done before the use of serum separation gel. Triglyceride can be detected by the following method, which is fast, simple and easy to operate   1. Instruments and reagents: automatic biochemical analyzer, triglyceride (trig) reagent and calibration solution, 5ml disposable sterile syringe, Desheng serum separation gel blood vessel, inferior serum separation gel blood vessel of a certain brand, traditional common blood vessel, 0.9% sodium chloride injection.   2. Methods: traditional common blood collecting vessels were used as control group A, Desheng serum separated blood collecting vessels were used as control group B, and inferior serum separated blood collecting vessels of a certain brand were used as experimental group C. each group randomly selected 10 tubes of blood collecting vessels, each tube added 5ml 9% sodium chloride injection, placed in 37 ℃ water bath for 15min, centrifuged at 3000r / min for 10min, the above tubes were measured on the automatic biochemical analyzer, and the test results were recorded. Compared with the results, TG could not be detected in the blood collection vessels of group A and group B, but different concentrations of TG could be detected in each tube of group C. through this method, the contrast test can be done quickly and accurately The quality of separating glue in blood collection vessel was evaluated.

Blood testing has become an indispensable means to detect diseases. At this time, patients often say, "I took several tubes of blood today, yellow, green, purple and blue. I just checked blood. Why do I take so many tubes?"   Actually, it's about the items you need to check. For general physical examination, such as blood routine, blood glucose, blood lipid, liver function, renal function, various tumor markers, etc., all need to be analyzed through blood detection, and different colors of blood collection vessels represent different kinds of additives and test purposes. Therefore, the items that cannot be detected together should be divided into multiple blood collection vessels. What do the colorful blood vessels represent? Here is to introduce the significance of various colors of blood vessels.   1. Yellow head cap tube (coagulation promoting tube): mainly used for serum biochemical (liver function, renal function, blood glucose, blood lipid, myocardial enzyme, electrolyte, amylase, etc.), thyroid function, drug detection, tumor markers, PCR, serum immunology detection, etc.   2. Purple head cap tube (EDTA-K2 anticoagulant tube): used for general Hematology (blood routine) examination, partial PCR items and glycosylated hemoglobin detection.   3. Green head cap tube (heparin anticoagulant tube): heparin tube is generally used for emergency biochemical and hemorheological tests.   4. Blue head cap tube (sodium citrate hydrochloride 1:9 anticoagulant tube): it is mainly used for the examination of coagulation items (prothrombin time, thrombin time, activated partial thrombin time, fibrinogen, etc.).   5. Black head cap tube (sodium citrate 1:4 anticoagulant tube): generally used for ESR detection.   6. Red cap tube (without additives): used very rarely, similar to yellow cap tube, mainly used for serum biochemical drug detection, serum immunology detection, etc.   Desheng biochemical specializes in the R & D, production and sales of vascular additives, in vitro diagnostic reagents, biological buffers and luminescent substrates. In the aspect of blood vessel additive, it has formed independent intellectual property rights and professional production and research capacity. To provide products and raw material solutions for more than 100 domestic and foreign manufacturers. The anticoagulant series products of blood samples include heparin sodium, heparin lithium, trisodium citrate, EDTA dipotassium, EDTA Tripotassium, potassium oxalate, etc.; the anticoagulant series products of blood samples include blood coagulant powder, blood coagulant, etc.; the materials used in the pretreatment of blood samples include separation gel, silicide, etc., and the anticoagulant tubes can also be provided for customers.